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Citations for Bioline :
251 - 300 of 419 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Quantitative PCR were performed on 20ng cDNA using and SensiFAST Probe Hi-ROX kits (BioLine) on a Quant Studio III (Applied BioSystem) ...
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bioRxiv - Biochemistry 2022Quote: Two versions of arsB were PCR amplified using VELOCITY DNA Polymerase (Bioline; catalog #BIO-21098) following manufactures recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed using the SensiFAST SYBR Lo-ROX Kit (BIO-94020, Bioline). Primers are shown in Supplementary Table 1 ...
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bioRxiv - Genetics 2024Quote: ... All genotyping PCRs were carried out using MyTaq™ Red Mix (Bioline, Cat# BIO-25043) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... All three PCR reactions contain 10 µL of MyTaq HS Red mix (BIO-25048, Bioline), 2-3 µL of the crude extract and 1 µL of each primer (10 µM ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Real-time quantitative PCR was performed using the SensiFAST™ SYBR® No-ROX Kit (Bioline) combined with a LightCycler® 480 Real-Time PCR System (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... each PCR assay was set-up with nuclease-free water as the negative control (Bioline, UK), and positive controls were not included due to financial constraints ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR was performed using C1000 Touch Thermal Cycler (BioRed) and SYBR mix (Bioline, GmbH, Germany), using validated primer sets (Herber et al ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was carried out using 5x PyroTaq qPCR mix Plus EvaGreen (CMB Cultek Molecular Bioline) in a QuantStudio5 machine (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The tagmented DNA was added to a PCR reaction with 2x MyTaq mix (BIO-25041; Bioline) with primers that add library specific indexes and amplified for 10 cycles ...
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bioRxiv - Microbiology 2021Quote: ... Four oligonucleotides were designed [40] for PCR using MyTaq Red Mix 1X (Bioline, Cat#BIO-25043), according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2022Quote: ... TE-genome junction validation PCRs were performed using MyTaq HS DNA Polymerase (Bioline, Cat#: BIO-2111). Reaction mixes contained 5μL 5× MyTaq Reaction Buffer ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR reactions were carried out using SYBR Green (SensiMix SYBR Low-ROX Kit) (BIOLINE) on 7500 MicroAmp Fast Optical 96-Well Reaction Plate (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative-RT PCR was then performed using the SensiFAST SYBR Green Hi-ROX master mix (Bioline). Primers were designed (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed using SensiMix SYBR Low-ROX Kit (Bioline; annealing temperature – 60°C) in a Lightcycler 480 384-well plate (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... Marker-exchange plasmids were generated by ligation of the PCR products in α-select cells (Bioline) using the SLIC cloning method (41) ...
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bioRxiv - Molecular Biology 2020Quote: ... target regions were amplified by PCR using 50 ng genomic DNA in MyTaq Red mix (Bioline) according to manufacture instructions (primers are shown in Table S3) ...
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bioRxiv - Microbiology 2021Quote: qRT-PCR was performed using the SensiFAST Probe Lo-ROX One-Step kit (Bioline Meridian, Australia) with a reaction volume of 20µl ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA (100 ng) was analysed by quantitative PCR using SensiFASTTM SYBR Lo-ROX Kit (Bioline) in the 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... Expression levels of target genes were determined by RT-PCR using SensiMix SYBR No-Rox (Bioline) with the primers shown in Supplementary Table 1 ...
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bioRxiv - Pathology 2023Quote: ... All real-time PCR reactions were performed in 20 μL volumes containing 10 μL Immomix (Bioline) and 5 μL of DNA template ...
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bioRxiv - Plant Biology 2023Quote: The subsequent amplification step of the PCR was performed using MangoTaq™DNA Polymerase (Bioline, Belgium) and the primers LCO1490 and HCO2198 designed by Folmer et al. ...
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Local adaptation of Arabidopsis thaliana in a small geographic region with mild environmental clinesbioRxiv - Plant Biology 2023Quote: ... Reverse transcriptase quantitative PCR (RT-qPCR) reactions were performed with the SensiFast SYBR Green Mastermix (Bioline) on a Bio-RAD cfx96 machine ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was carried out using 5x PyroTaq qPCR mix Plus EvaGreen (CMB Cultek Molecular Bioline) in a QuantStudio5 machine (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1x MyTaq™ reaction buffer and 1.0 U of MyTaq™ DNA polymerase (Bioline Meridian Bioscience) adjusted to a total volume of 20µL ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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bioRxiv - Genomics 2019Quote: ... 8 µl of DpnII-digested products was amplified by PCR with MyTaq Red Mix (Bioline #BIO-25044) and 1.25 µM primers Adr-PCR-Rand1 in a total volume of 40 µl ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative Real-Time PCR was performed using a SensiFAST™ SYBR® No-ROX Kit (Bioline; www.bioline.com). Fluorescence was monitored with a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Real-time RT PCR was performed using the SensiFAST™ Probe Lo-ROX one-step kit (Bioline). In each reaction the primers final concentration was 600nM and the probe concentration was 300nM ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR of protonema samples was conducted using the SensiFastTM SYBR No-ROX Kit (Bioline, Luckenwalde, Germany) in a LightCycler 480 (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... Table 1) were used in a pre-optimised PCR master mix (BioMix™, Bioline, Meridian Bioscience; Australia) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Real-time RT-PCR was conducted with SensiFAST™ Probe Lo-ROX One-Step Kit (Bioline, 78005) and analyzed with the 7500 Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: ... For conventional PCR we used a commercial master mix 2x My Taq HS Mix (Bioline Bio-25046). PCR amplifications were carried out in the T-100 Thermal Cycler (Biorad) ...
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bioRxiv - Cancer Biology 2022Quote: PCR reactions were performed in 25 μl reactions using the MyTaq Red Mix (Bioline, Meridian Life Science). The RT-PCR reaction conditions were optimised for each primer pair and are designated as Condition A to D ...
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bioRxiv - Microbiology 2022Quote: ... mutants were selected by PCR screening of colonies grown on LBN + 5% sucrose using Taq polymerase (Bioline) and the primers RSTX_For (5’-GTCACGGGTTGATTGATTCGCAT-3’ ...
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were diluted and 5 μl was used for genotyping PCR using MyTaq DNA polymerase (Bioline) with indicated DNA primers ...
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bioRxiv - Genetics 2022Quote: Genotyping was performed using standard PCR protocols for MyTaq HotStart Red Mix (Bioline BIO- 25048, Memphis, TN) with primers listed in Supplementary Table 7.
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bioRxiv - Molecular Biology 2020Quote: ... Both PCR reactions were carried out with 25 µl MyTaq Red mix (Bioline cat. no. BIO-25044), 0.5 µM of each primer and 50 µl final volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 8 μl of DpnII-digested products was amplified by PCR with MyTaq Red Mix (Bioline #BIO-25044) and 1.25 μM primers Adr-PCR-Rand1 in a total volume of 40 μl ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was carried out using the Bioline SensiFASTTM Probe Lo-ROX Kit (Bioline, Catalogue no.-84005) and Stratagene Mx3005P (Agilent Technologies) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT–PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with 1 µl of complementary DNA (cDNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were generated by PCR amplification of sequences of interest with Velocity DNA Polymerase (Bioline, #BIO-21099) using primers synthesized by IDT or restriction enzyme digestion with NEB High-Fidelity enzymes ...
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bioRxiv - Microbiology 2022Quote: ... RT-PCR reaction was setup using SensiFast Probe No-ROX One- Step Kit (Bioline, Cat. BIO-76005) using the following primers/probes ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplex PCR amplification was performed in a 10 μL reaction containing 1X MyTaq HS Red Mix (Bioline), 10 ng DNA template ...
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bioRxiv - Plant Biology 2023Quote: ... and real time PCR reactions were completed in duplicate for each sample using a SensiMix SYBR (Bioline); 95◻ for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Real- time PCR was performed using 2x SensiMix SYBR and Fluorescein Kit (Bioline, QT615-05, Luckenwalde, Germany), 20ng cDNA and pre-tested gene-specific primer sets (listed in Supplementary table 3) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA using Sensifast Hi-Rox Sybr kit (Bioline). Two qRT-PCR reactions (technical replicates ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR was performed with the Bio-Rad CFX96 cycler using the SensiFAST™ SYBR® (Bioline). Gene expression for occludin (Ocln) ...
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bioRxiv - Microbiology 2023Quote: ... both samples were subjected to PCR with human GAPDH-specific primers41 and MyTaq polymerase (Bioline, Taunton, MA). Resulting PCR amplicons were visualized by agarose gel electrophoresis ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... Cells were then lysed in hypotonic lysis buffer containing 0.16 U µl−1 Ribosafe RNase inhibitors (Bioline), 2 mM PMSF and SIGMAFAST Protease Inhibitor Cocktail tablets ...