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Citations for Bioline :
1 - 50 of 419 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The DamID PCR buffer consisted of 1.36x MyTaq™ Buffer (Bioline) and 1.06 μM of the DamID PCR primer (Adr_PCR ...
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bioRxiv - Microbiology 2021Quote: ... The PCR mix was composed of 5 μL of 5X MyTaq red PCR buffer (Bioline), 1.25 μL of 25 mM MgCl2 ...
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bioRxiv - Neuroscience 2019Quote: A 1.0 mm section of caterpillar horn from second-third instar larvae was harvested and placed in a 96-well plate with 10.0 µl of MyTaq™ Extract-PCR Kit Lysis buffer (2 µl Buffer A; 1 µl Buffer B; 7 µl ddH2O, Modified protocol from Bioline; Meridian Life Sciences; United States). Tissue was homogenized using a wet toothpick to quickly press the horn tissue to the side of the well or by cutting the horn multiple times in the lysis buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... The different PCR polymerases and buffers were supplied by Meridian Bioscience (formerly Bioline). The oligonucleotides were supplied by Merck (formerly Sigma-Aldrich) ...
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bioRxiv - Genetics 2023Quote: ... 10X PCR buffer from BIOTAQ™ DNA Polymerase (Bioline, Cat. No. BIO-21040) was diluted to 1X and supplemented with proteinase K (Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were visualised by electrophoresis in TAE buffer on a 1.5% agarose gel (Bioline, London, UK) stained with 1x SYBR Safe (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR reactions were carried out using 1.5 mM MgCl2 and 1X NH4 buffers for 1U of BioTaq polymerase (Bioline), 0.2 mM dNTP mix ...
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bioRxiv - Cancer Biology 2023Quote: For each reaction 1μL of gDNA solution was suspended in a PCR tube with 2μL of MyTaq red reaction buffer (5x; Bioline), 1µL of pooled primers/oligonucleotides (final concentration of each primer 10μM or 5μM for Ubc-Cre) ...
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bioRxiv - Genetics 2019Quote: ... Ear punches taken to identify animals during routine colony maintenance were placed directly into Buffer A from the MyTaq Extract-PCR kit (Bioline).
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bioRxiv - Microbiology 2023Quote: ... A fragment of each gene was individually amplified by polymerase chain reaction (PCR) from the leaf DNA extracts using the MyTaq reaction buffer and polymerase (Bioline) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... For methylated DNA amplification by PCR each one of these products was mixed with 118 μl of DamID PCR buffer and 2 μl (10 units) of MyTaq™ DNA polymerase (Bioline) and were aliquoted at 40 μl in 4 different 0.2 ml PCR tubes ...
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bioRxiv - Immunology 2021Quote: ... The master mix was prepared with 2.5 mL of 2X buffer containing Taq-polymerase was prepared from the TaqMan RT-PCR kit (Bioline #BIO-78005). From the kit ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR amplifications were performed in 40 µl reactions at standard concentrations (1.5 mM MgCl2, 0.2 mM dNTP, 1x Biolase PCR buffer, 0.5 U Taq (Bioline USA, Inc., Randolph, MA), 0.5 µM each primer ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed colony PCR using BioRed PCR mix (Bioline) to screen for genome modifications ...
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bioRxiv - Genomics 2023Quote: ... PCR products purified with Isolate II PCR and Gel Kit (BIOLINE) and cloned into the entry vector pCR®8/GW/TOPO (#250020 Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... After PCR verification in agarose gel and PCR clean-up using the ISOLATE II PCR and Gel Kit (Bioline), the remaining plasmid template was digested with DpnI at 37°C for 3h ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified using the PCR Isolate II PCR and Gel Kit (Bioline) or by ExoSAP (for primers see Table S4) ...
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bioRxiv - Immunology 2020Quote: ... PCRs were performed with the IMMOLASE™ DNA polymerase PCR kit (Bioline), and the success of amplification was assessed by agarose gel electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... and quantitative real-time-PCR (qRT-PCR) reactions were performed in triplicate (Bioline) and monitored by a Roche Lightcycler 96 ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using the 2× SYBR Green PCR Master Mix (Bioline) with the CFX96 detection system and analyzed with CFX Manager softward (Bio-rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting PCR products were purified on columns (Isolate II PCR Kit (BIO-52059, Bioline)) that are suitable for >50 bp DNA fragments ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified by using the Isolate II PCR and gel kit (Bioline), analyzed by agarose gel electrophoresis and then sequenced (GATC Biotech ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR was performed using MyTaq polymerase (Bioline; PCR program in Supplementary Table 3). If embryos showed the desired deletions ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR was performed using MyTaq polymerase (Bioline; PCR program in Supplementary Table 3). If embryos showed deletions ...
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bioRxiv - Molecular Biology 2024Quote: ... real-time PCR was conducted using the SensiFAST SYBR real-time PCR kit (Bioline). The obtained data were normalized to the human endogenous control (GAPDH ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.2 x Hifi Buffer (Bioline), 0.1 x SYBR Green (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR amplicon band was cut from the gel and isolated by PCR Isolate II PCR and Gel Kit (Bioline, cat. no. BIO-52060) and lastly bead-purified ...
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bioRxiv - Zoology 2019Quote: ... PCR products were purified with an ISOLATE II PCR and Gel Kit (Bioline, BIO-52059) according to the manufacturers guidelines ...
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bioRxiv - Genomics 2023Quote: ... PCR products were purified using the ISOLATE II PCR and Gel Kit (Bioline, BIO-52060) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were purified using the ISOLATE II PCR and Gel Kit (Bioline, BIO-52060) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... PCR products were extracted from the agarose gel with ISOLATE II PCR and Gel Kit (Bioline) and Sanger sequenced.
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bioRxiv - Microbiology 2021Quote: ... the 15 μl PCR reaction mixture contained 1x MyTaq™ Red PCR Mix (Bioline, South Africa), 0.2 μM primers ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR amplification (Bioline, Taunton, MA). The libraries were sequenced by multiplexing 8 libraries per lane on the Illumina HiSeq2500 sequencer ...
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bioRxiv - Microbiology 2020Quote: ... MyTaq Red PCR Mix (Bioline, UK) and 200 nM sense/anti-sense primers and amplification following 35 cycles of 95°C ...
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bioRxiv - Bioengineering 2023Quote: ... My Taq PCR master mix (Bioline) that included buffer ...
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bioRxiv - Plant Biology 2024Quote: ... PCR was performed using MyTaq (Bioline) DNA Polymerase in reactions containing 30ng of DNA template ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1X NH4 Buffer (Bioline, Boston, MA), 3 mM MgCl (Bioline ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5μl of 10 x Buffer (Bioline), 1.25μl of 50mM MgCl2 (Bioline) ...
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bioRxiv - Plant Biology 2019Quote: ... The 15 μl PCR cocktail consisted of 7.5 μl of PCR MyTaq Red Mix (Bioline, GmbH, Germany), 1.0 μl of genomic DNA template (70 ng/μl) ...
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bioRxiv - Microbiology 2020Quote: PCR products were purified using a commercial kit (Isolate II PCR and Gel Kit, Bioline, London, UK) and sent for sequencing (Macrogen Europe ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR or RT-PCR analyses were performed using Brilliant III SYBR green QPCR master mix (Bioline) with gene-specific primers on a Applied Bioscience StepOnePlus qPCR machine (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... The 15 µl PCR reaction mixture contained 1x of MyTaq ™ Red PCR Mix (Bioline, South Africa), primers at 0.2 µM ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Bioline Isolate II PCR and Gel kit (Bioline, Cat. No. BIO-52060). Samples were sequenced using the Illumina Miseq platform ...
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bioRxiv - Pathology 2019Quote: ... PCR amplicons of each sample were pulled together and purified using Isolate II PCR and gel Kit (BIOLINE) and about 100 ng of DNA was used in library preparation ...
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bioRxiv - Neuroscience 2021Quote: ... In short myTaq extract-PCR kit (Bioline) was used to amplify a 300 bp fraction of the ath5 gene containing the lakritz mutation using the following primers ...
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bioRxiv - Physiology 2020Quote: ... using SYBR Green PCR master mix (Bioline). Data is represented as 2^-ΔCt compared to average of PC1/3 in healthy group ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions were conducted using MyTaq (Bioline) DNA polymerase ...
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bioRxiv - Genetics 2020Quote: ... or MyTaq™ Extract-PCR Kit (Bioline). gDNA was prepared from blastocysts in a 20 µL solution of 185.5 mM pH 8.3 Tris-HCl ...
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Psi promotes Drosophila wing growth through transcriptional repression of key developmental networksbioRxiv - Developmental Biology 2020Quote: ... PCR using MyTaq polymerase (Bioline BIO-21113) was performed with 3 long extension cycles followed by 17 short extension cycles as described (Vogel ...
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bioRxiv - Genetics 2020Quote: ... Genotyping was performed by PCR of genomic tail DNA using the Extract PCR kit (Bioline, cat. No. BIO-21127). All mice were kept on C57BL/6J (The Jackson Laboratory ...