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Citations for Bioline :
201 - 250 of 419 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2021Quote: ... PCR amplification was performed using 12.5 µL of MyTaq HS Mix (Bioline, US) hot-start polymerase ...
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bioRxiv - Genomics 2022Quote: ... Dam-methylated DNA was then amplified by PCR with MyTaq polymerase (Bioline®), sonicated to an average size of 300bp and the DamID adaptors were removed by AlwI (NEB ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR was performed using the SensiMix SYBR Low-ROX kit (Bioline) and a LightCycler® 480 (Roche) ...
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bioRxiv - Systems Biology 2022Quote: ... followed by quantitative RT-PCR (RT-qPCR) using the SensiMix SYBR kit (Bioline). Expression analysis of genes of interest was performed on a Rotor-Gene Q (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... The primers were tested with PCR using MyTaq DNA polymerase (Bioline, London, UK), with annealing temperature of 61 °C and an extension time of 30 s ...
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bioRxiv - Microbiology 2023Quote: PCR for cloning purposes was performed using the proofreading Bio-X-ACT (Bioline) or Phusion (NEB ...
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bioRxiv - Immunology 2023Quote: RT-PCR was done using the RedTaq DNA Polymerase (Bioline, Memphis, TN, USA) following the recommended protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR was carried out with a SensiFAST SYBR No-Rox Kit (Bioline) on a CFX96 Real-time PCR Detection System ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative RT-PCR reactions were performed with SensiFAST SYBR Hi-ROX kit (Bioline) and gene-specific primers (Supplemental File S2) ...
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bioRxiv - Plant Biology 2023Quote: ... and further purified with Isolate II Gel and PCR Clean-up Kit (Bioline). Libraries were then generated from fragmented DNA (Covaris ...
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bioRxiv - Plant Biology 2024Quote: ... The qRT-PCR amplification was performed with SensiMixTM SYBR NO-Rox Kit (Bioline) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were separated by gel electrophoresis on 2% DNA grade agarose (Bioline) gels in TAE buffer (40 mM Tris Acetate ...
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bioRxiv - Molecular Biology 2024Quote: ... A volume of GRASPS buffer A1 containing 0.16 U/μl RNase inhibitor (Bioline), 2 mM PMSF and cOmplete™ EDTA-free Protease Inhibitor Cocktail corresponding to approximately three times the volume of the cell pellet (600 μl ...
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bioRxiv - Physiology 2021Quote: ... Quantitative PCR was performed with the SensiFAST™SYBR® No-Rox Kit (Bioline) using 1.25 ng per reaction ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using the SensiFAST SYBR No-ROX One-Step Kit (Bioline) and primers listed in Table S1 ...
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bioRxiv - Neuroscience 2019Quote: ... A nested PCR (Table S2) using MyTaq™ HS Red Mix (Bioline; United States) was necessary for a sufficient amplification of a 920 bp region flanking the gRNA1 target site ...
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bioRxiv - Molecular Biology 2019Quote: ... All reaction products were purified with the Isolate II PCR kit (Bioline USA, Inc.). These libraries underwent nick translation and amplification ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting cDNA was amplified and quantified using SYBR Green PCR Master Mix (Bioline) in QuantStudio 6 Flex ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time quantitative PCR was performed using the Sensimix SYBR low-rox kit (Bioline) and the primers in Supplementary Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... A 25 µl reaction mixture contained 1x MyTaq Red PCR Mix (Bioline, South Africa), four species-specific forward primers and reverse primer IS711 (Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... A 25 µl PCR reaction contained 1x MyTaq ™ Red Mix (Bioline, South Africa), eight species-specific forward and reverse primers at a final concentration of 6.25 µM (Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCR (qPCR) reactions (20 μl) included 1X SensiMix SYBR green master mix (Bioline), 0.5 μM of each primer and 5 μl template cDNA (used at 1:200 dilution in RNase-free water) ...
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bioRxiv - Microbiology 2022Quote: ... PCR used a total volume of 20 µL with 10 µL BioMix (BioLine, UK) 1 µL of each primer (10 µM) ...
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bioRxiv - Pathology 2019Quote: ... and subjected to qRT-PCR using SensiFAST™SYBR Lo-ROX kit (Bioline, USA). Data were analyzed in triplicates ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PCR used a total volume of 20 μL with 10 μL BioMix (BioLine, UK) 1 μL forward primer (10 μM) ...
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bioRxiv - Plant Biology 2020Quote: ... The final PCR products were visualized on a 1% (w/v) agarose gel (Bioline) by electrophoresis at 90 V for 1 h ...
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Isonicotinamide extends yeast chronological lifespan through a mechanism that diminishes nucleotidesbioRxiv - Biochemistry 2021Quote: ... and quantitated by real-time PCR reactions using SensiMix SYBR Hi-ROX Mastermix (Bioline) run on a StepOne Plus thermocycler (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using 10 μl MyTaq Red mix (Bioline, cat. no. BIO-25044), 1 μM of each TAC0017 and TAC0018 primers ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed with the SensiFAST SYBR No-Rox kit (Bioline, BIO-98005) and μM of each primer ...
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bioRxiv - Genomics 2023Quote: ... PCR reactions were prepared using the MyTaq DNA polymerase kit (Bioline, Cat# BIO-21111). Reactions contained a 5× MyTaq Buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantitative RT-PCR analyses were performed using SensiFAST™ SYBR® kit (Bioline) on an Applied Biosystems StepOne Plus instrument ...
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bioRxiv - Immunology 2023Quote: ... 1x MyTaq Red Reaction buffer red and 0.5 μl of MyTaq DNA polymerase (Bioline) on SimpliAmp thermal cycler (Applied Biosystems ...
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bioRxiv - Biophysics 2021Quote: Genotyping of ClC-7 KO U2OS clone was performed using an Extract PCR-Kit (Bioline) with the following primers ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative RT-PCR analyses were performed using Brilliant III SYBR green QPCR master mix (Bioline) with gene-specific primers according to the manufacturer’s protocol and with the Applied Bioscience StepOnePlus qRT-PCR machine (Life Technologies) ...
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bioRxiv - Genetics 2021Quote: ... A total of 14 PCR cycles were performed using MyTaq Red Mix (#BIO-25043; Bioline), yielding ~30 μg barcodes ...
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bioRxiv - Microbiology 2020Quote: ... The PCR reaction volumes are as follows: 12.5 μL MyTaqTM Master Mix (Bioline, United Kingdom), 10 μL molecular-grade water (G-Biosciences ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative PCR (qPCR) was performed using the Sensimix SYBR low-rox kit (Bioline, Memphis, USA) on a 7500 real-time PCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR reaction containing SensiMix™ SYBR Green® No-ROX Kit (QT650-20, Bioline) was run on a 7900 Real time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR assays were performed using PyroTaq EvaGreen mix Plus (ROX) (CulteK Molecular Bioline, Spain) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was carried out using the SensiFAST SYBR Hi-ROX kit (Bioline, BIO92005). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative RT-PCR analyses were performed using Brilliant III SYBR Green QPCR master mix (Bioline) with gene-specific primers according to the manufacturer’s protocol and the Applied Bioscience StepOnePlus qRT-PCR machine (Life Technologies) ...
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bioRxiv - Physiology 2019Quote: ... Quantitative real-time PCR were carried out using SensiFAST™ SYBR No-ROX (2X, Bioline) on a LightCycler 480 System (Roche Diagnostics ...
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bioRxiv - Genomics 2021Quote: ... A DNA contamination PCR was performed using MyTaq™ DNA Polymerase (Bioline cat # BIO-21105) and primers for ACTB to check for the presence of genomic DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were carried out with ∼100 ng genomic DNA in MyTaq Red mix (Bioline) and purified using the PCR Isolate II PCR and Gel Kit (Bioline ...
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bioRxiv - Genomics 2020Quote: ... PCRs were carried out as 25 µl reactions containing 0.03 units of MyTaq™ (Bioline), 1 × buffer and 0.2 μM of forward and reverse primers and 2 μl of template DNA ...
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bioRxiv - Microbiology 2020Quote: ... The amplified product was purified using the ISOLATE II PCR and Gel Kit (Bioline, Australia) and cloned into the BamHI and SacI sites of the E ...
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bioRxiv - Microbiology 2021Quote: ... The final PCR product (sequencing library) was purified with JetSeq Beads (Bioline, Cat # BIO-68031), followed by library quality control check ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed using the SensiFASTTM Probe Lo-ROX One-Step kit (Bioline, UK). Primers and probe sequences ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed using the SensiFASTTM Probe Lo-ROX One-Step kit (Bioline, UK). Primers and probe sequences ...
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bioRxiv - Cancer Biology 2021Quote: ... RT-quantitative PCR (RT-qPCR) was performed using a SensiMix SYBR kit (Bioline, #QT605-05) on an Applied Biosystems ViiATM7 Real-Time PCR machine ...