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Citations for GE Life Sciences :
401 - 450 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto PVDF 0.45 µm or nitrocellulose membranes (GE Healthcare) in a Tris-glycine transfer buffer containing 20% methanol ...
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bioRxiv - Genomics 2021Quote: ... Proteins were then transferred on Amersham Protran 0.2 NC membranes (GE Healthcare). Membranes were blocked overnight in Tris-buffered-saline supplemented with 0.5% Tween 20 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... The separated proteins were transferred to 0.45 μm Nitrocellulose membrane (GE Healthcare). The membrane was blocked by 5% non-fat milk in TBST buffer (TBS buffer with 0.1% Tween 20 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were separated and transferred onto nitrocellulose membrane (GE Healthcare Life Sciences). The membrane was washed in tris-buffered saline containing 0.1% Tween (Sigma ...
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bioRxiv - Genetics 2021Quote: ... prior to protein transfer to Amersham Protran nitrocellulose blotting membrane (GE Healthcare) using a Mini Trans-Blot Cell (BioRad ...
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bioRxiv - Immunology 2022Quote: ... proteins were transferred onto polyvinylidene difluoride (PVDF) membrane (GE Healthcare, IL, USA). The membrane was blocked with 5% dry milk in PBS containing 0.1% v/v Tween 20 (PBST ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were transferred by western blotting to HyBond ECL membrane (GE Healthcare) and detected using anti-HisTag (MBL Life Science ...
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bioRxiv - Microbiology 2021Quote: ... proteins were transferred to nitrocellulose membrane (GE Healthcare Life Sciences, Pittsburgh, PA) using Trans-Blot SD semi-dry transfer cell (BioRad ...
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bioRxiv - Immunology 2020Quote: ... The separated proteins were transferred onto a PVDF blotting membrane (GE Healthcare) and blocked for 1h with 5% skimmed milk in 1×TBST (Tris-buffered saline containing 0.05% Tween-20 ...
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bioRxiv - Microbiology 2019Quote: ... Separated proteins were transferred onto nitrocellulose membrane (0.45μm pore size, GE Healthcare) at 45V for 2 hours ...
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bioRxiv - Pathology 2021Quote: ... After the protein bands were transferred onto PVDF membranes (GE Healthcare, UK), the blots were probed with an RSV NP specific (1:5000 diluted ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were transferred to PVDF membranes (Amersham Hybond™-P, GE Healthcare) by semi-wet blotting (XCell II™ Blot Module ...
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to 0.45 µm pore size nitrocellulose membranes (GE Healthcare) either by wet blot in transfer buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were electrotransferred onto a PVDF membrane (AmershamTM HybondTM, GE Healthcare) in 25 mM Tris ...
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bioRxiv - Physiology 2021Quote: ... Proteins were visualized after transfer onto a PVDF blotting membrane (GE Healthcare) and incubation with specific primary antibodies using horseradish peroxidase-conjugated secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Transfer of protein bands onto a nitrocellulose membrane (GE Healthcare, Amersham, UK) was performed at 15 V overnight/ 300mA for 3-5 h or semi-dry at 90V for 1.5 h ...
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bioRxiv - Immunology 2022Quote: ... Proteins were then blotted onto polyvinylidene fluoride membranes (GE Healthcare, Buckinghamshire, UK), blocked with skimmed milk and incubated with primary antibodies (Table S1 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred onto 0.45 μm pore size nitrocellulose membranes (GE Healthcare Life Sciences] using NuPAGE™ Transfer Buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were then transferred to nitrocellulose membranes (GE Healthcare, Boston, MA, USA) using the semi-dry system PerfectBlue Electro Blotter (Peqlab ...
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bioRxiv - Physiology 2022Quote: ... Resolved proteins were electrotransferred onto polyvinylidene difluoride (PVDF) membrane (GE Healthcare, USA) at 100V ...
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bioRxiv - Pathology 2023Quote: ... Proteins were blotted onto nitrocellulose membranes (Amersham, GE Healthcare, Chicago, IL, USA). Western blotting was performed with the use of primary antibodies listed in Table 2 ...
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bioRxiv - Cell Biology 2023Quote: ... we transferred the proteins onto nitrocellulose membranes (GE Healthcare, Little Chalfont, UK) using the wet transfer method for 1 hour at 250 mA (4 °C) ...
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bioRxiv - Cancer Biology 2022Quote: ... Separated proteins were transferred to a nitrocellulose membrane (Amersham Protran, GE Healthcare) with of 0.45 μm pore size at 100 V for 1 h on ice in transfer buffer ...
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bioRxiv - Plant Biology 2023Quote: ... followed by the protein transfer to a Protran nitrocellulose membrane (GE Healthcare). To control for equal loading of whole protein ...
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bioRxiv - Molecular Biology 2023Quote: ... The separated proteins were transferred to the nitrocellulose membrane (GE Healthcare, USA). Primary antibodies were used for probing HIF1α (#14179 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were immunoblotted on 0.2 µm nitrocellulose membranes (GE Healthcare Amersham, 10600006) using Pierce Methanol-free Transfer buffer (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... Proteins were visualized after transfer onto a PVDF blotting membrane (GE Healthcare) and incubation with specific primary antibodies using horseradish peroxidase-conjugated secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Separated proteins were transferred to a nitrocellulose membrane (GE Healthcare, Freiburg, Germany) in transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2019Quote: ... All His-tagged proteins were purified from the cell culture medium using HisTrap Excel column (GE Healthcare, catalog 17371206) and eluted using buffer containing 50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... The eluted proteins from Ni-NTA column were loaded onto a Hi-load 16/60 Superdex 200 (GE Healthcare) gel filtration column pre-equilibrated with 25mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by Ni-NTA immobilized metal-affinity chromatography using His-Trap HP columns (GE Healthcare) followed by size exclusion chromatography ...
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bioRxiv - Immunology 2023Quote: ... Protein TLR827–827-PA-His was purified with IgG beads (Cat#17-0969-01, GE Healthcare, Connecticut, CT, USA) and resuspended in 200 μl of NT2 buffer 66 and used for miRNA-protein co-IP ...
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bioRxiv - Biochemistry 2023Quote: ... most of the proteins were loaded onto a Hi-trap Q HP anion exchange column (GE Healthcare, cat. 17115401). The final purification step involved using a size exclusion column ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 93-207 Bcl-2) with a C-terminal 6His tag and purified using Ni2+ IMAC (GE Healthcare, Fastflow). Similarly ...
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bioRxiv - Cell Biology 2021Quote: Tag-free syndapin I and III were generated by expressing both proteins in the pGEX-6P vector (GE Healthcare) and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: Clarified extracts containing hexahistidine fusion proteins were passed through a 5 mL Ni-Sepharose (GE Healthcare #17-5268-01) packed column at 0.5 mL/min using an ÄKTAprime Plus chromatography system ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified extracts containing GST fusion proteins were passed through a 5 mL GSTrap column (GE Healthcare #17-5131-02) at 0.1 mL/min using an ÄKTAprime Plus chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare). Finally ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... GST-hMeCP2 or GST-OGT fusion proteins were immobilized on glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) and washed three times with GST binding buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence microscopy data of intracellular GFP fusion proteins were collected using a DeltaVision microscope system (GE Healthcare Inc., USA) equipped with a pco.edge 4.2 sCMOS camera (PCO ...
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bioRxiv - Cancer Biology 2023Quote: The purified GST-tagged fusion protein WDR522-334 (100 μg) was bound to Glutathione Sepharose 4B (GE Healthcare, 28952360) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells as described previously.13 The protein was purified using glutathione-Sepharose 4B beads (GE Healthcare) concentrated and flash-frozen in GST buffer [25 mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: The purified rhGFAT2-his protein was subjected to a size exclusion chromatography using a Superdex 200 column (GE Healthcare, USA). The column was equilibrated with 1 CV of 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... The protein was purified via a two-step protocol of Ni-affinity using a 1ml His-Trap column (GE Healthcare) in Ni-affinity buffer [50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... the proteins were first purified from bacterial cell lysates using His-Trap HP (Ni-NTA) columns (GE Healthcare Life Sciences), as per the manufacturer’s instructions ...