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Citations for GE Life Sciences :
501 - 550 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Immunology 2021Quote: The scCl5 was purified using a two-steps protocol using first immobilized metal affinity chromatography (IMAC) to enrich for His tagged protein (1 ml HisTrap, GE Healthcare) followed by anion exchange (1 ml HiTrapQ ...
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bioRxiv - Developmental Biology 2022Quote: ... The media was buffer exchanged with PBS and His-tagged proteins were captured with 5 mL HisTrap Excel columns (GE Healthcare), washed with 20 mM imidazole and eluted with 300 mM imidazole containing PBS buffer ...
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bioRxiv - Biophysics 2019Quote: ... The protein was further purified using reverse IMAC with the cleaved protein being loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Biophysics 2021Quote: ... was purified by 1 M NaCl containing Tris buffer using AKTATM Pure protein purification system with Hi-Trap Q-FF anion exchange column (GE healthcare). 0.3% H2O2 was added to the purified proteins ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by Ni-NTA immobilized metal-affinity chromatography (IMAC) using five milliliter His-Trap HP columns (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and used for recombinant protein purification either by using AKTA FPLC or on His-Trap columns (GE Healthcare) as per the manufacturer guidelines or by manually purifying pre-equilibrated Ni-NTA-agarose (Qiagen) ...
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bioRxiv - Biochemistry 2024Quote: ... protein was again passed over Nickel resin to remove His-tagged protein and further purified by size exclusion chromatography (Superdex 75 10/300g, GE healthcare) into 20mM Tris.HCl 7.5 ...
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked in 2% ECL Prime™ Blocking Agent (GE Healthcare, RPN418) in TBST (Tris-Buffered Saline (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... The solubility tag was cleaved with TEV protease and the protein further purified by size exclusion chromatography (Superdex75 GL300/10/300; GE Healthcare) at 4°C in a SEC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were digested with PreScission protease to remove tags and further purified by superose-6 increase column (GE Healthcare) in TBS buffer with 0.1% digitonin or 40 μM GDN ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were isolated from the medium exploiting the affinity of the 6xHis tag for the HisTrap Excel (GE Healthcare, USA) affinity column ...
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bioRxiv - Developmental Biology 2019Quote: Full-length dP75 protein with GST tag was purified from BL21 E.coli by using Glutathione Sepharose 4B GST-tagged protein purification resin (GE healthcare, 17075601) and following the protocol in GE healthcare webpage ...
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bioRxiv - Biophysics 2020Quote: ... was separated from the remaining tag protein and protease by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). The unbound tag-free protein was collected and protein concentration was determined by absorbance (A280 ...
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bioRxiv - Biochemistry 2022Quote: ... TEV and residual affinity tag was removed by passing the protein through a Superdex 200 Increase 10/300 GL (GE Healthcare) in SEC buffer (30 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to the protein solution to a final concentration of 1.3 μM and incubated for 15 minutes at room temperature in order to cleave off His10-SUMO tag and the protein solution was applied on HisTrap 1 HP (GE Healthcare) loaded with Zn2+ and pre-equilibrated with storage buffer ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Uncleaved protein and cleaved hexahistidine tag were removed from the eluted mixture with a HisTrap HP 5 mL column (GE Healthcare) in PBS with 25 mM imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... Separation of cleavage tags and aggregated proteins was performed using size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20□mM citric acid pH 5.0 ...
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bioRxiv - Microbiology 2023Quote: ... the negatively charged N-terminal His- and BAP-tag fused MBP protein was separated from HphA by ion-exchange chromatography with HiTrap Q HP 5ml column (GE Healthcare). The flow-through ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... The tag-free protein was further purified by size-exclusion chromatography on a Hiload 16/60 Superdex 75 column (GE Healthcare). Mutants and ZF truncations were expressed and purified using the same procedure as for the wild-type proteins ...
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bioRxiv - Genetics 2021Quote: ... and proteins were transferred to a nitrocellulose membrane (Amersham Protran 0.45 NC, GE Healthcare).
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bioRxiv - Synthetic Biology 2019Quote: Western blotting was carried out following protein transfer onto a nitrocellulose membrane (GE Healthcare) on a TE 77 PWR Semi-Dry Transfer Unit (45 mA/gel and 30 min with a NOVA Blot kit ...
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bioRxiv - Microbiology 2019Quote: ... proteins were transferred onto a nitrocellulose membrane (Amersham Protran 0.45 µM NC (GE Healthcare)) for one hour at 400mA ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were resolved by SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). StrepII-tagged proteins were detected using Strep-Tactin AP conjugate (IBA ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes (Amersham Protran, 0.45 μm; GE Healthcare Life Sciences) and incubated with primary α-GFP (monoclonal ...
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bioRxiv - Cell Biology 2021Quote: Protein samples were separated by SDS-PAGE and transferred onto nitrocellulose membranes (GE Healthcare) using the Trans-Blot Turbo System (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then blotted on a 0.45-µm pore size nitrocellulose membrane (GE Healthcare) using a wet/tank transfer blotting system (Biorad ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were separated and transferred to PVDF membrane (GE Healthcare Cat No-10600023) using semi-dry transblot apparatus (Hoefer ...
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bioRxiv - Physiology 2022Quote: ... Separated proteins were transferred onto 0.2 µm Nitrocellulose membranes (catalog no 10600015, GE Healthcare) in Tris-Glycine (25 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... proteins were blotted onto a nitrocellulose membrane (Hybond-C Extra, GE Healthcare, Munich, Germany) Ponceau S staining of the blotted membrane was performed to assure equal amounts of proteins ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were then wet-transferred to a 0.2 μm Nitrocellulose membrane (Amersham GE Healthcare) at 150 V for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... and proteins were transferred to a nitrocellulose membrane (Amersham Protran 0.45 nitrocellulose, GE Healthcare) in transfer buffer (50 mM Tris-OH ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were electrophoretically transferred onto nitrocellulose membranes (Amersham Protran 0.45 μm NC; GE Healthcare) at 100 V for 180 min on ice in the transfer buffer without SDS/EDTA ...
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bioRxiv - Plant Biology 2019Quote: ... and the separated protein bands were electroblotted onto a polyvinylidene difluoride membrane (GE Healthcare). Different amount of proteins were used for each western blotting ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were electrophoretically transferred onto nitrocellulose membranes (Amersham Protran 0.45 μm NC; GE Healthcare) at 100 V for 180 min on ice in transfer buffer [48 mM Tris/HCl ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were electrophoretically transferred onto nitrocellulose membranes (Amersham Protran 0.45 μm NC; GE Healthcare) at 90 V for 90 min on ice in transfer buffer [48 mM Tris/HCl ...
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bioRxiv - Pathology 2020Quote: ... Proteins were transferred to the Hybond-PVDF-LFP membrane (Amersham, GE Healthcare Life Sciences) and blocked with 5% skimmed milk in 1X Tris-buffered saline ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were separated and transferred to PVDF membrane (GE Healthcare Cat No-10600023) using semi-dry transblot apparatus (Hoefer ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated by SDS-PAGE and were transferred onto PVDF membranes (GE Healthcare) for immunoblot analysis ...
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bioRxiv - Physiology 2020Quote: ... The separated proteins were electro-transferred to PVDF membranes (GE healthcare, Cat no. 10600021). These were subsequently blocked with 5% milk in Tris-buffered saline with 0.1% Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2019Quote: ... Then proteins were transferred onto an Amersham Protran western blotting nitrocellulose membrane (GE Healthcare) by semi-dry transfer ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was transferred to a polyvinylidene difluoride (PVDF) membrane (Amersham, GE Healthcare Life Sciences) by using a semi-dry transfer on Bio-Rad Transblot turbo transfer system (Bio-Rad ...