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Citations for GE Life Sciences :
651 - 700 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... coli knockout collection (Dharmacon, GE Healthcare). Hedamycin was obtained from the National Cancer Institute’s Developmental Therapeutic Program (NCI DTP ...
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bioRxiv - Plant Biology 2023Quote: ... coli vector pGEX-2TK (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2019Quote: ... and anti-His (GE Healthcare, Chicago, IL, USA) primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with argon-flushed 100 mM sodium phosphate pH 7.4 buffer ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with 20 mM sodium phosphate buffer pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... and purified using His-Trap HP (GE Healthcare) followed by gel filtration using HiLoad 16/60 Superdex 75 columns on an ÄKTA Xpress ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a His-Trap column (GE Healthcare), and eluted by applying a linear imidazole gradient from 20 mM to 200 mM over 30 column volumes ...
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bioRxiv - Plant Biology 2024Quote: ... purified on a His-Trap column (GE Healthcare) following the manufacturers’ instructions ...
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bioRxiv - Genetics 2021Quote: ... The gels were transferred for 2 hours onto nitrocellulose membranes (GE Healthcare Biosciences, Pittsburgh, PA). Detection was performed by diluting primary antibodies in TBS supplemented with 0.1% Tween and either 5% BSA or 5% non-fat dry milk ...
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bioRxiv - Plant Biology 2020Quote: ... and 2 mM EDTA at pH 7.4) and blotted onto Hybond-N+ membrane (GE Healthcare) using a Minifold I slot blot system (GE Healthcare) ...
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bioRxiv - Physiology 2019Quote: ... Upon blocking the membrane with 2% (w/v) Amersham ECL Prime Blocking Reagent (GE Healthcare) for an hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... RNA (2 μg) of each group was spotted on a Hybond-N+ membrane (GE Healthcare), followed by UV cross-linking (120 mJ/cm2 ...
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bioRxiv - Biophysics 2023Quote: ... His6-tagged fusion proteins were isolated from the resulting supernatant by affinity chromatography using Ni2+ Sepharose High-Performance beads (GE Healthcare, Pittsburgh, PA). Bound fusion proteins were washed with 200-300 mL of column Buffer A ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were blotted onto a nitrocellulose membrane (Amersham Protran 0.2 NC, GE Healthcare life sciences) for 1-2h at 300 mA ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Molecular Biology 2020Quote: ... protein-RNA complexes were immobilized on Protran nitrocellulose membrane (0.45 μm pore size, Whatman, GE Healthcare) and unbound RNA immobilized on Hybond Nylon membrane (Amersham ...
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bioRxiv - Plant Biology 2020Quote: ... Separated proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, United Kingdom) using a wet tank unit (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare 10600019 or 10600001). Appropriate transfer of proteins was verified by staining of membranes with the Ponceau S solution (Roth 5938.1) ...
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bioRxiv - Biochemistry 2020Quote: ... After electrophoresis the proteins were transferred to Nitrocellulose membranes (Amersham Protran Premium Nitrocellulose, GE Healthcare, #10600048) via wet transfer at a current of 45V for approximately 20 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 μm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine) ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 μm NC) at 90 V for 90 min on ice in transfer buffer (48 mM Tris-HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Cancer Biology 2020Quote: ... The separated proteins were transferred to a 0.2 µm nitrocellulose membrane (Amersham Protran, GE Life Sciences) using wet transfer method for 2 h at 200 mA ...
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bioRxiv - Cancer Biology 2020Quote: ... Membrane-immobilized protein detection used ECL Western Blotting Detection Reagents (GE Healthcare RPN2106; Millipore Sigma GERPN2106).
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were separated by SDS-PAGE and were transferred onto PVDF membranes (GE Healthcare Life sciences) for immunoblot analysis ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were run on a 10 % SDS-PAGE and immobilized on a nitrocellulose membrane (GE Healthcare) using the Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, United Kingdom) in a wet tank unit (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for separation of proteins which were transferred to nitrocellulose blotting membrane (GE Healthcare, #10600002). Following washing with 0.05% Tris buffered saline-Tween (TBST) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto Amersham Hybond 0.2 PVDF blotting membranes (Cat# 10600021, GE Healthcare Life Sciences) in Tris glycine buffer (25 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated on a 10% SDS-PAGE and immobilized on a nitrocellulose membrane (GE Healthcare) using the Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... Proteins were transferred to a nitrocellulose membrane (Amersham Protran 0.45 µm NC, GE Healthcare, Buckinghamshire, UK) using a semi-dry Western Blot system ...
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bioRxiv - Genetics 2021Quote: ... Protein lysates were resolved by 12% SDS-PAGE and transferred to PVDF membrane (GE Healthcare AmershamTM) using a Trans-Blot® SD Semi-Dry Transfer Cell (BioRad) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were blotted on a 0.45 μM pore size nitrocellulose membrane (GE Healthcare, Chicago, IL, USA) using a Transblot SD semi-dry transfer system (Bio-rad ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 8-15% SDS gels and transferred to a nitrocellulose membrane (GE Healthcare) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine) ...
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bioRxiv - Neuroscience 2021Quote: ... Biotinylated membrane proteins were purified using Streptavidin High Performance Spintrap columns (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cell Biology 2020Quote: ... The proteins in the gel were transferred to polyvinylidene fluoride (PVDF) membrane (GE Healthcare Life Sciences). The membranes were blocked with 2% milk (Marvel ...
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bioRxiv - Developmental Biology 2022Quote: ... The proteins were then transferred onto a Protran Nitrocellulose Membranes (0.45 µm pore size, GE Healthcare) via Power Blotter-Semi-dry Transfer System (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Abcam prestained mid-range protein ladder (Ab116027) and 0.45 µM Amersham Hybond PVDF membrane (GE Healthcare) were used ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred (100 V, 1 hr) onto PVDF (polyvinylidene difluoride) membranes (GE Healthcare Life Sciences) using 1X Tris/Glycine buffer (in-house 50X solution containing 250 mM Tris and 1920 mM Glycine) ...
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bioRxiv - Physiology 2022Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred onto nitrocellulose membranes (GE Healthcare). The nitrocellulose membranes were blocked with 5% BSA in TBST (TBS containing 0.1% Tween20 ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 90 V for 90 minutes on ice in transfer buffer (48 mM Tris base and 39 mM glycine supplemented with 20% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Proteins were transferred onto an Amersham™ Protran™ 0.45 μm nitrocellulose membrane (GE Healthcare, UK). Membranes were blocked with BlueBlock PF (Serva Electrophoresis GmbH ...
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bioRxiv - Microbiology 2023Quote: ... The proteins separated by the SDS-PAGE were transferred to a nitrocellulose membrane (GE life Sciences). The membrane was then incubated for 1 h at room temperature or overnight at 4 °C in blocking buffer (5% non-fat milk powder diluted in TBST (20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated using 12 %SDS-PAGE and blotted onto a polyvinylidene difluoride membrane (GE Healthcare). The membrane was blocked in 5% milk/PBS for 1 h followed by overnight incubation with mouse monoclonal anti-TFAM antibody (Abcam ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred overnight onto nitrocellulose membranes (Whatman, GE Healthcare Life Sciences, Buckinghamshire, UK) in a 1 litre tank containing transfer buffer at 30 V.
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (Amersham Hybond ECL; GE Healthcare, RPN303D, Merck, Darmstadt, Germany). After blocking in 5% (w/v ...
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bioRxiv - Developmental Biology 2023Quote: ... The proteins were then transferred from the gel to the PVDF membrane (GE Healthcare Life-Science) in Bio-Rad vertical gel Transfer Apparatus using Transfer buffer (2.5mM Tris base ...
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bioRxiv - Plant Biology 2023Quote: Protein samples were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). After being blocked in 1 × PBST buffer containing 5% skimmed milk ...
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bioRxiv - Microbiology 2024Quote: ... and the proteins were then transferred onto nitrocellulose blotting membranes (NC) (10600001; GE Healthcare Life Science). The membranes were blocked with 5% non-fat dry milk and 0.1% Tween 20 for 30 min at 37°C ...
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bioRxiv - Genetics 2024Quote: ... The proteins were electrophoresed on 10% SDS□PAGE gels and transferred to nitrocellulose membranes (GE Healthcare). The blots were blocked in 5% milk and incubated with primary antibodies overnight at 4 °C ...