Labshake search
Citations for GE Life Sciences :
51 - 100 of 4253 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... Then the cells were disrupted in French press and the lysate was extracted and purified using Ni2+-NTA affinity column of [KTA Pure (GE healthcare). We used 20 mM imidazole containing buffer as binding buffer and 250 mM imidazole containing sodium phosphate buffer as elution buffer ...
-
bioRxiv - Cell Biology 2020Quote: GST-tagged proteins (Retromer, SNX3, WASHC2C-5 and WASHC2C-21) were purified by incubating clarified lysate with glutathione (GSH) Sepharose 4B (GE Healthcare) beads ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared via ultracentrifugation and his-tagged protein purified on the AKTÄ Pure on the HisTrap HP column (GE Healthcare). Fractions of interest were second round purified via size exclusion chromatography on the Superdex 200 column (GE Healthcare) ...
-
bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
-
bioRxiv - Pathology 2020Quote: ... the vector was purified from the cell lysate using a iodixanol density gradient followed by anion exchange chromatography (HiTrap Q-FF column, GE Healthcare). The scAAV9-CB-SMN vector was finally resuspended and concentrated in DPBS on a centrifugal filter unit (Amicon® Ultra-15 ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was first purified by Immobilized Metal Affinity Chromatography (IMAC) by loading the clarified lysate onto a 5 mL Nickel column (GE Healthcare) pre-equilibrated in buffer A ...
-
bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on two 5 mL-HiTrap heparin columns (GE Healthcare) at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on a 5 mL-HiTrap heparin column (GE Healthcare) at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysates were cleared by centrifugation and the soluble protein fraction was affinity-purified on a HisTrap Excel column (GE Healthcare). After elution in a 0–300 mM imidazole gradient ...
-
bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
-
bioRxiv - Cell Biology 2023Quote: ... rPF4 was purified from soluble fractions of cell lysates by affinity chromatography using a 5-ml HiTrap heparin-agarose column (GE Healthcare). Endotoxin-free rPF4 was prepared as previously described 3 ...
-
bioRxiv - Cell Biology 2023Quote: GST fusion protein was similarly purified using affinity chromatography by incubating bacterial cell lysate with glutathione-Sepharose 4B beads (#17075601; GE Healthcare) according to the manufacturer’s instructions and eluted from beads with elution buffer (50 mM Tris-HCl ...
-
bioRxiv - Microbiology 2019Quote: ... FtsZ or ΔCTL were then purified from the lysate using anion exchange chromatography column (HiTrap Q HP 5 ml, GE Life Sciences), followed by ammonium sulfate precipitation (at 20% - 30% ammonium sulfate saturation) ...
-
bioRxiv - Biochemistry 2020Quote: ... MALX-E-NirF was purified by loading the cleared cell lysate onto a 5ml MBPtrap HP column (GE Healthcare, Chicago, United States) which was eluted with a gradient to 20 mM maltose ...
-
bioRxiv - Immunology 2020Quote: ... Lysates were centrifuged at 14,000 rpm and proteins purified by affinity chromatography using a Ni2+ column (1 mL GE Healthcare HisTrap HP) and imidazole gradient elution in an AKTA Start protein purification system ...
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
-
bioRxiv - Neuroscience 2022Quote: ... were purified from bacterial lysate by affinity chromatography on a GSTrap FF column (Cat. No. 71-5016-96 AM, GE Healthcare, Chicago, IL) followed by dialysis against multiple changes of phosphate buffered saline (PBS) ...
-
bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... lysates were loaded into a ResourceS (GE Healthcare) cartridge and eluted in a gradient of 0 to 500 mM NaCl in 50 mM ammonium acetate ...
-
bioRxiv - Molecular Biology 2021Quote: ... Lysates were incubated with lgG sepharose (GE Healthcare) at 4 °C for 2 hrs ...
-
bioRxiv - Biochemistry 2019Quote: ... Bacterial lysates were collected as described above and the lysates were incubated with 1 ml Glutathione Sepharose 4B (GE Healthcare, UK) at 4°C for 1 hour ...
-
bioRxiv - Cell Biology 2019Quote: ... Lysates were mixed with glutathione sepharose beads (GE Healthcare) and incubated overnight at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... Cleared lysates were incubated with IgG-Sepharose (GE Healthcare) at 4 °C for 2 h and eluted by TEV protease (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... lysates were incubated with streptavidin sepharose beads (GE Healthcare) overnight while rotating at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated with Protein A Sepharose (GE Healthcare) for 1-2 hr at 4°C and bound proteins were washed extensively with lysis buffer and eluted with SDS sample buffer with boiling.
-
bioRxiv - Biophysics 2019Quote: ... Lysate was loaded on to HisTrap HP (GE Healthcare), His-RanQ69L was eluted in binding buffer with 500mM Imidazole ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lysates were loaded onto HisTrap crude column (GE Healthcare) on FPLC system (AKTA pure) ...
-
bioRxiv - Biochemistry 2021Quote: ... cleared lysate was applied on GST-Trap (GE Healthcare) before extensive washing with ly-sis buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... Cleared lysates were incubated with IgG Sepharose (GE Healthcare), washed with buffer Y and eluted by overnight TEV cleavage.
-
bioRxiv - Cell Biology 2023Quote: ... lysates were incubated with glutathione Sepharose beads (GE Healthcare) coated with the other interaction partner for 30min at 4°C on a rotary wheel ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell lysates were incubated with Streptactin II (GE healthcare) affinity beads for 2 hours at 4°C while rotating ...
-
bioRxiv - Bioengineering 2020Quote: ... Clarified lysate loaded onto HiTrap Q HP column (GE Healthcare) and eluted through salt gradient using low-salt buffer (as described above ...
-
bioRxiv - Molecular Biology 2021Quote: ... The lysates were loaded on HisTrap HP column (GE Healthcare) and after extensive washing the recombinant proteins were eluted by imidazole gradient ...
-
Structural determinants of protocadherin-15 elasticity and function in inner-ear mechanotransductionbioRxiv - Biophysics 2019Quote: ... The cleared lysates were loaded onto Ni-Sepharose (GE Healthcare), eluted with denaturing buffer supplemented with 500 mM imidazole and refolded as indicated in Table S3 using MWCO 2000 membranes (Spectra/Por) ...
-
bioRxiv - Cell Biology 2020Quote: ... Centrifuge-cleared lysate was applied to Ni sepharose (GE healthcare), washed with 50 mM HEPES (pH 7.4) ...
-
bioRxiv - Plant Biology 2021Quote: ... protein lysates were incubated with glutathione Sepharose beads (GE Healthcare) in E buffer for 2 h ...
-
bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were incubated with prewashed glutathione sepharose (GE Healthcare) and gently mixed with rotation for 1 h ...
-
bioRxiv - Cell Biology 2019Quote: ... Lysates were mixed with protein G sepharose beads (GE Healthcare) bound to α-PP2A-Aα or control IgG ...
-
bioRxiv - Molecular Biology 2021Quote: ... the cleared lysate was applied to Streptactin beads (GE Healthcare) in batch and incubated for 1 h at 4°C with rotation ...
-
bioRxiv - Cell Biology 2019Quote: ... Lysates were incubated with IgG sepharose 6 beads (GE Healthcare) in XB buffer (100 mM KCl ...
-
bioRxiv - Biophysics 2019Quote: ... Cleared lysate was loaded onto a glutathione-sepharose (GE Healthcare) column (GST-column ...
-
bioRxiv - Microbiology 2021Quote: ... Cleared lysates were incubated with glutathione Sepharose 4B (GE Healthcare), washed with wash buffer (20 mM Tris pH 8.5 [pUL21-GST] or 7.5 [GST-PP1γ] ...
-
bioRxiv - Biochemistry 2021Quote: ... cleared lysate was incubated with glutathione-Sepharose resin (GE Healthcare) for several hours before washing the resin first with PBS buffer containing 1% (v/v ...
-
bioRxiv - Microbiology 2020Quote: ... Cell lysates were mixed with either StrepTactin sepharose (GE Healthcare) for PorEStrep immunoprecipation ...
-
bioRxiv - Microbiology 2019Quote: ... Cleared lysates were then incubated with glutathione sepharose (GE healthcare) for 4 hours at 4°C and ...
-
bioRxiv - Microbiology 2021Quote: ... The lysates were loaded on HisTrap HP column (GE Healthcare) and after extensive washing the recombinant protein was eluted by imidazole gradient ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with pre-equilibrated streptavidin beads (GE Healthcare) for 4 hr ...
-
bioRxiv - Neuroscience 2023Quote: ... The cleared lysates were loaded onto Ni-Sepharose (GE Healthcare) and eluted with denaturing buffer or non-denaturing buffer supplemented with 500 mM imidazole ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were precleared with Gelatin Sepharose 4B beads (GE Healthcare) for 2 h and then incubated with Streptavidin Sepharose High Performance beads (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was incubated with glutathione sepharose (GE Healthcare) and then washed with 50 mM Tris-HCl pH 7.5 ...