Labshake search
Citations for GE Life Sciences :
251 - 300 of 4253 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Filtered lysate was used for protein purification with an Äkta start system (GE Healthcare). Lysis buffer containing 20 mM imidazole and wash buffer with 40 mM imidazole was used ...
-
bioRxiv - Developmental Biology 2019Quote: ... The total lysate was pre-cleared with equilibrated Protein A Sepharose beads (GE Healthcare) for 2 hours at 4 degrees ...
-
bioRxiv - Cell Biology 2020Quote: ... Clarified lysate was loaded onto a 5 ml HisTrap FF Crude column (GE Healthcare), washed in a buffer containing 20 mM HEPES-KOH ...
-
bioRxiv - Biochemistry 2020Quote: ... Cell lysate was loaded onto a Strep-Trap HP column (5 mL, GE Healthcare), equilibrated according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... The lysate was loaded onto a 5 ml HisTrap FF Crude column (GE Healthcare) equilibrated with wash buffer containing 50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: Purification for WT DnaC from clarified lysates was performed using Ni-Sepharose (GE Healthcare) affinity resin ...
-
bioRxiv - Biophysics 2021Quote: ... The cell lysate was loaded to a His-trap column (GE Healthcare, IL, USA), washed with phosphate buffered saline (PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... CRY2-PHR was isolated from the soluble cell lysate by glutathione sepharose (GE Healthcare) using a buffer containing 50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... The clarified lysate was then loaded on HisTrap HP (5 ml) cartridge (GE Healthcare), and eluted through a linear gradient of Buffer D ...
-
bioRxiv - Biochemistry 2020Quote: ... The lysate was loaded on a HiTrap Q FF (5 ml) cartridge (GE Healthcare), pre-equilibrated with the same buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... Clarified lysate was then flowed over a 5-ml Ni-NTA column (GE Healthcare), before being washed with 50 mM Tris pH 8 ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was cleared and incubated with equilibrated glutathione sepharose 4B beads (GE Healthcare) overnight at 4°C ...
-
bioRxiv - Biophysics 2021Quote: ... The lysate was incubated with 5mL of washed and packed GST resin (GE Healthcare) for 1 hour at 4 °C with gentle rocking ...
-
bioRxiv - Biophysics 2022Quote: ... The lysate was clarified by centrifugation and loaded onto a HisTrap column (GE Healthcare). The column was washed with the imidazole buffer (20 mM NaH2PO4 ...
-
bioRxiv - Biophysics 2022Quote: ... The clarified lysate was loaded onto a 5mL His Trap HP column (GE Healthcare) pre-equilibrated in buffer A ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cleared lysate was loaded onto a 5 mL HisTrap sepharose HP column (GE Healthcare) equilibrated with buffer A ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant of cell lysate was loaded onto a Ni column (GE Healthcare, USA) after filtration with a 0.22 µm filter ...
-
bioRxiv - Biochemistry 2021Quote: ... Clarified lysates were incubated with Ni-NTA 6 Fast Flow beads (GE Life Sciences), with gentle rotation ...
-
bioRxiv - Biophysics 2021Quote: ... The cleared lysate was loaded onto a 5 ml Ni-NTA column (GE Healthcare) connected to a FPLC system (GE healthcare ...
-
bioRxiv - Cell Biology 2019Quote: ... Lysates were then incubated with protein G sepharose beads (GE Healthcare, 17-0618-01) for 2–3 h at 4°C and beads were washed 3 times with diluted lysis buffer (dilution of 1:3 in dH2O ...
-
bioRxiv - Cell Biology 2019Quote: ... cell lysates of these complexes were firstly treated with GST agarose beads (GE Healthcare). And then the protein-bound beads were transferred into gravity columns and treated with 3C protease overnight at 4 °C ...
-
bioRxiv - Biochemistry 2019Quote: ... The clear lysate was firstly passed through a 5 mL TALON column (GE Healthcare). After extensive washing ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The resulting filtered lysate was applied to a 5 mL HisTrap column (GE Healthcare) on an ÄKTA FPLC system (GE Healthcare ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Clarified cell lysate was loaded onto a GSTrap FF column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 10 column volumes of binding buffer (1x PBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... Clarified lysates were loaded onto an affinity column (HisTrap HP 5 ml; GE Healthcare). Eluted protein was further purified using an anion exchange column (HiTrap Q HP 5 ml ...
-
bioRxiv - Plant Biology 2020Quote: ... The clarified lysate was incubated with Ni-sepharose 6 fast flow beads (GE Healthcare) equilibrated in buffer A ...
-
bioRxiv - Biochemistry 2019Quote: ... The soluble lysate was bound in batch-mode to Glutathione Sepharose 4B (GE Healthcare), then washed and eluted with 50 mM HEPES buffer pH 7.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... The cleared lysate was loaded onto a pre-equilibrated HisTrap Crude FF (GE Healthcare) column ...
-
bioRxiv - Biochemistry 2021Quote: ... 650μg-1mg of the lysate was added to Protein A-Sepharose beads (GE Healthcare) pre-incubated for 3-7 h with rabbit anti-FLAG antibody (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2020Quote: ... Cell lysates were passed through a 5 mL chelating sepharose FF column (GE Healthcare) charged with nickel and equilibrated in binding buffer ...
-
bioRxiv - Developmental Biology 2021Quote: ... the lysates were applied to Sephacryl S400 HR spin columns (GE Healthcare Life Sciences), and ribosome-protected fragments were purified using the Zymo RNA Clean & Concentrator-25 kit (Zymo Research) ...
-
bioRxiv - Biochemistry 2020Quote: ... Lysates were loaded on to a Ni-sepharose IMAC gravity flow column (GE healthcare), washed with 2 column volumes of wash buffer (buffer A supplemented with 45 mM imidazole) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was applied to a 5 mL HisTrap HP column (GE Healthcare) and washed with Ni wash buffer containing 25 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2020Quote: ... the lysate of cells was loaded onto a HiTrap Chelating HP column (GE Healthcare) equilibrated with a buffer containing 20 mM imidazole ...
-
bioRxiv - Molecular Biology 2020Quote: ... chromatin lysates were incubated for 3 hours with protein-G sepharose beads (GE Healthcare). Beads were washed 3× with low-salt buffer (150 mM NaCl ...
-
bioRxiv - Microbiology 2021Quote: ... The clarified cell lysate was first passed over Ni-NTA Sepharose resin (GE Healthcare). The resin was washed with wash buffer (lysis buffer containing 20-40 mM imidazole) ...
-
bioRxiv - Cell Biology 2021Quote: ... The clarified lysate was added to 20 μL Glutathione Sepharose 4B resin (GE Healthcare) that had been washed three times in TUBE lysis buffer ...
-
bioRxiv - Cancer Biology 2022Quote: ... Sonicated cell lysate was loaded into a 5 ml HisTrap HP column (GE Healthcare) and purified by nickel affinity chromatography ...
-
bioRxiv - Cell Biology 2022Quote: ... Clarified bacterial lysates were incubated with glutathione sepharose beads 4B (GE Healthcare Life Sciences). Beads with immobilized GST-SH3 domains were extensively washed in LB1 buffer and then added to lysates from HeLa cells ...
-
bioRxiv - Plant Biology 2022Quote: ... the lysate was loaded onto amylose column MBPTrap HP (GE Healthcare, #28-9187-78) prewashed with 5 ml Column Buffer (CB) ...
-
bioRxiv - Microbiology 2023Quote: ... The cell lysate was loaded on a Ni-sepharose FF HiLoad column (GE Healthcare), equilibrated in 20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biophysics 2023Quote: ... Extracted protein lysates were mixed with Streptavidin Sepharose High-Performance beads (GE Healthcare, USA) at 4°C overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a HiTrap DEAE_FF 5 ml column (GE Healthcare) pre-equilibrated with 0.1 mM ZnCl2/20 mM HEPES ...
-
bioRxiv - Microbiology 2023Quote: ... Clarified lysates were then incubated with A28 antisera-conjugated protein A beads (GE Healthcare) at 4 °C for 4 h with rotation at 15 rpm ...
-
bioRxiv - Biochemistry 2023Quote: ... The clarified lysates were loaded onto a 5 mL NiNTA HisTrap column (GE Healthcare). The column was then washed with 25 mL of wash buffer (4 M guanidine hydrochloride ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysate was circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-040901) charged with 100 mM CoCl2 for 1 hour ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell lysate was then loaded onto glutathione SepharoseTM 4 Fast Flow resin (GE Healthcare), and washed with Tris buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... the cell lysates in buffer D was incubated with Ni-NTA resin (GE Healthcare), washed using buffer D supplemented with 20 mM imidazole ...
-
bioRxiv - Biophysics 2023Quote: ... Clarified lysates were loaded onto a Superose 6 10/300 GL column (GE Healthcare), attached to an analytical high-performance liquid chromatography system with in-line fluorescence detection (Shimadzu) ...
-
bioRxiv - Cell Biology 2023Quote: ... clarified lysates were applied to a 5 ml HisTrap™ HP column (GE Healthcare) and washed with lysis buffer followed by a buffer containing 20 mM Tris-HCl pH 8.0 at 4°C ...