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Citations for GE Life Sciences :
101 - 150 of 763 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins in 10 mM acetate buffer (pH 4.5, GE Healthcare) was then injected over the chip surface at a flow rate of 30 μl/min to couple the amino groups of the recombinant proteins to the carboxymethyl dextran matrix ...
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bioRxiv - Genetics 2020Quote: ... Membrane was incubated in Rapid-hyb hybridization buffer (Amersham, GE healthcare) at 37°C for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... NrnA – 6xHis was buffer exchanged using PD-10 columns (GE Healthcare) into storage buffer (40 mM Tris pH 8 ...
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bioRxiv - Bioengineering 2022Quote: ... FPLC-purified biotinylated proteins (ligands) in HBS-P+ Buffer (GE Healthcare) were captured on a Streptavidin (SA ...
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bioRxiv - Neuroscience 2022Quote: ... Monomeric aSyn was buffer exchanged using PD10 midi traps (GE Healthcare) into the same buffers ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS buffer and imaging using Typhoon FLA 9000 (GE Healthcare). Densitometric analysis was performed using FIJI software.
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bioRxiv - Molecular Biology 2023Quote: ... MSN was buffer exchanged (NAP-5 column; GE Healthcare Life Sciences) and diluted to 20 µM in ITC buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 M Na3PO4 (#28-9030-59, Ab Buffer Kit, GE Healthcare). Beads were incubated at 4°C on a rotator ON ...
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bioRxiv - Biochemistry 2023Quote: ... equilibrated with buffer A using a ÄKTA Pure system (GE Healthcare). LF and its mutants were eluted by a linear NaCl gradient using buffer A and 20 mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2024Quote: ... Buffer exchange was performed via PD-10 desalting column (GE Healthcare) to 50mM MES pH 5.0 for experiments and storage ...
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Genomics 2020Quote: ... PCR products were purified using Strepdavidin Sepharose High Performance beads (GE healthcare). The beads bound to DNA were washed in 70% ethanol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR-products were purified using Illustra ExoProStar 1-Step (GE Healthcare) following the instructions from the manufacturer ...
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bioRxiv - Genetics 2019Quote: ... PCR products were cleaned with GFX Illustra DNA Purification Kit (GE Healthcare); standardized aliquots were sent to Macrogen (http://www.macrogen.com ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR-product was inserted into the pGEX-5X-1 (GE Healthcare) according to the protocol above ...
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bioRxiv - Biochemistry 2021Quote: ... PCR products were bound to Streptavidin Sepharose HP (GE Healthcare Life Sciences) for immobilization ...
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bioRxiv - Neuroscience 2022Quote: ... The PCR product was bound to Streptavidin Sepharose High Performance (GE Healthcare), purified ...
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bioRxiv - Microbiology 2019Quote: ... PCR fragments were then labelled using Megaprime DNA Labeling Systems (GE Healthcare). Primers used are listed in Table 1.
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bioRxiv - Microbiology 2022Quote: ... the PCR reactions were loaded on Hi-Trap Streptavidin column (GE Healthcare). By addition of 60 mM NaOH ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was bound to Streptavidin Sepharose High Performance (GE Healthcare), purified ...
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bioRxiv - Immunology 2020Quote: ... Buffer exchange was then performed using a NAP-5 column (GE Healthcare) and protein amount quantified using a MicroBeta TriLux liquid scintillation counter ...
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bioRxiv - Molecular Biology 2021Quote: ... the buffer was exchanged via a PD-10 desalting column (GE Healthcare) into 10 mM HEPES-NaOH pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... in MES buffer and transferred to a PVDF membrane (Osmonics, GE Healthcare) by wet transfer at 100 V for 1 h in transfer buffer [25 mM Tris ...
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bioRxiv - Bioengineering 2019Quote: ... and PBS running buffer were purchased from BIAcore (GE Healthcare, Uppsala, Sweden). All other reagents used in the present work were of analytical grade and were applied without further purification.
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bioRxiv - Biochemistry 2019Quote: ... and the buffer was exchanged by gel filtration (GE Healthcare Life Sciences) into the degassed solution (20 mM HEPES [pH 6.5] ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 mM NaCl pH 7.5 buffer using a MicroCal ITC200 (GE Healthcare). The protein solutions (27 µM ...
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bioRxiv - Microbiology 2021Quote: ... followed by buffer exchange using HiPrep 26/10 desalting column (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... the reaction was buffer exchanged using a NAP-5 column (GE Healthcare) to remove excess nucleotide and salt ...
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bioRxiv - Cell Biology 2022Quote: ... buffer was exchanged to PBS with a PD10 desalting column (GE Healthcare). Proteins were quantified measuring UV absorption at 280nm and subsequently supplemented with 3mM dithiothreitol (DTT) ...
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bioRxiv - Biochemistry 2022Quote: ... After exchange into wash buffer using PD-10 desalting columns (GE Healthcare) His tags were cleaved with thrombin (1 U/mg EmrE3 ...
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bioRxiv - Plant Biology 2021Quote: ... The pellets were resuspended in buffer containing 7 M urea (GE Healthcare), 2 M thiourea (GE Healthcare) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were lysed 48h post-transfection with mammalian lysis buffer (GE Healthcare), then the luciferase substrate (Promega ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and resuspended them in Protein Extraction Buffer-V (GE Healthcare, Uppsala, Sweden) (urea ...
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bioRxiv - Biochemistry 2021Quote: ... and the buffer was exchanged using PD-10 column (GE Healthcare, USA) to Buffer-E (20 mM HEPES (pH 7.4) ...
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bioRxiv - Plant Biology 2020Quote: ... Hybridizations were performed at 65°C in Rapid-Hyb buffer (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The purified protein was buffer exchanged with PD-10 columns (GE Healthcare) into 100 mM potassium phosphate buffer pH 7.6 ...
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bioRxiv - Cell Biology 2023Quote: ... each sample was buffer exchanged a PD-10 desalting column (GE Healthcare) that had been equilibrated with RIPA buffer ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in Blocking Buffer containing 5 % Blocking for ECL (GE healthcare) in Tris buffer for two hours ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR product was purified on a MonoQ 10/100 column (GE Healthcare) with a 0.5–1 M NaCl gradient in 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... purified with mini columns (GFX PCR DNA & gel band purification kit, GE Healthcare) is described in this protocol (dx.doi.org/10.17504/protocols.io.brzsm76e ...
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bioRxiv - Microbiology 2020Quote: ... Purified PCR products were inserted into the pGEX-6P-1 vector (GE Healthcare) using BamHI and EcoRI restriction enzymes (New England Biolabs) ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR product was bound to Streptavidin Sepharose HP (GE Healthcare Life Sciences), after which the immobilized PCR products were purified ...
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bioRxiv - Genomics 2020Quote: ... PCR reactions were cleaned up using 1.5 volumes of SpeedBeads™ (GE Healthcare) in 2.5 M NaCl/20%PEG8000 and libraries size selected on a PAGE/TBE gels to 150–225 bp ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR products were purified either by ExoStar DNA purification reagent (GE Healthcare) or Wizard SV Gel and PCR Clean-Up System (Promega) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the PCR reaction was mixed with streptavidin beads (GE Healthcare #17-5113-01) and binding buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... purified with mini columns (GFX PCR DNA & gel band purification kit, GE Healthcare), and sequenced in ABI3500XL Series automatic sequencer (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR product was purified with ExoStar (GE Healthcare Life Sciences, Pittsburgh, PA, USA), labeled with BigDye Terminator v.3.1 (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... The biotinylated PCR products were immobilized on streptavidin-coated sepharose beads (GE Healthcare). DNA strands were separated using the PyroMark Q24 Vacuum Workstation ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was generated using Illustra RT-PCR ready-to-go beads (GE Healthcare) in a two-step reaction ...
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bioRxiv - Genetics 2023Quote: ... A radiolabeled PCR probe was prepared by random priming (GE Healthcare, Megaprime kit) using α-P32 dCTP and incubated with the membrane in commercial buffer (Ambion ...