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Citations for GE Life Sciences :
1 - 50 of 763 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... PCR reactions were performed with RTG PCR beads (GE Healthcare) in 25 μL final volume ...
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bioRxiv - Molecular Biology 2021Quote: ... and the assay buffer was HBS-EP+ buffer (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... that had been equilibrated in NiNTA A buffer followed by buffer exchange into SEC buffer using a 5 mL HiTrap® Desalting Column (GE Healthcare). Fractions containing protein of interest were pooled ...
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bioRxiv - Biophysics 2021Quote: ... followed by buffer exchange into lysis buffer using PD-10 desalting columns (GE Healthcare), His6-tag cleavage by TEV protease and subtractive Ni2+-NTA purification ...
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bioRxiv - Neuroscience 2022Quote: ... 1%IPG buffer (GE Healthcare)] ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using GFXTM PCR DNA and Gel Band Purification Kit (GE Healthcare). Restriction endonucleases and T4 DNA ligase were purchased from Thermo Fisher Scientific and PrimeSTAR DNA polymerase from Takara Bio ...
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bioRxiv - Biophysics 2019Quote: ... The other was buffer exchanged into SEC buffer using a PD-10 column (GE Healthcare The protein was then treated with 0.4 units of bovine kidney α-L-fucosidase (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... and the buffer was exchanged into final buffer by PD-10 desalting column (GE Healthcare). The final protein concentration was determined by UV-Vis absorption at 274 nm using an extinction coefficient of 2.8 cm-1mM-1 for tau187 and 5.8 cm-1mM-1 for 2N4R ...
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bioRxiv - Immunology 2022Quote: The antibodies were buffer exchanged into carbonate buffer using Nap5 columns (Illustra, GE Healthcare, 17085302) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Biotin-labelled PCR amplicons were captured by mixing PCR products with streptavidin-sepharose beads (GE Healthcare). Vacuum Prep Tool with filter probes was used to capture sepharose beads with biotin labelled PCR amplicons ...
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bioRxiv - Microbiology 2021Quote: ... The DNA of purified PCR products (GFX PCR DNA and Gel Band Purification Kit GE Healthcare) was sequenced using primer Map Tn5 B fwd.
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bioRxiv - Cell Biology 2021Quote: ... and the buffer was then exchanged to imidazole-free buffer using PD-10 Columns (GE Healthcare). Proteins were concentrated using 30K Amicon Ultra Centrifugal Filters (Merck ...
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bioRxiv - Biochemistry 2021Quote: ... using buffer B (GE Healthcare Biosciences) and a flow rate of 1 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting PCR products were purified with illustra GFX PCR DNA & Gel Band Purification Kit (GE Healthcare) followed by 2-propanol precipitation ...
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bioRxiv - Cancer Biology 2021Quote: PCR products were purified using an ExoSAP-IT PCR Purification Kit (US78200, GE Healthcare, Piscataway, NJ, USA) and sequencing reactions were performed with the Big Dye Terminator V1.1 Kit (4336805 ...
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bioRxiv - Cancer Biology 2023Quote: PCR products were purified using an ExoSAP-IT PCR Purification Kit (US78200, GE Healthcare, Piscataway, NJ, USA) and sequencing reactions were performed with the Big Dye Terminator V1.1 Kit (4336805 ...
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bioRxiv - Biochemistry 2020Quote: ... the proteins were buffer exchanged into the appropriate buffer using an Illustra NAP-5 column (GE Healthcare).
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bioRxiv - Evolutionary Biology 2022Quote: ... Polymerase chain reactions (PCRs) were prepared using PuReTaq Ready-To- Go PCR Beads (GE Healthcare, Chicago, Illinois, USA). Each 25-μl reaction volume contained 19 μl distilled water (dH2O) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified for sequencing by Illustra GFX PCR DNA and Gel Band Purification kit (GE Healthcare). Primers and purified DNA were sent to GATC biotech (Lightrun method ...
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bioRxiv - Immunology 2020Quote: ... the PCR product was purified with the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, UK) and eluted in dH2O ...
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bioRxiv - Immunology 2020Quote: ... Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit, GE Healthcare, USA). In brief ...
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bioRxiv - Biochemistry 2022Quote: RIP2K1-317 buffer exchange in buffer F was done by using a PD-10 desalting column (GE healthcare). 5mM MgCL2 and 2 mM ATP were added to the kinase sample ...
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bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
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bioRxiv - Biochemistry 2023Quote: ... Aurora A protein was thawed and buffer exchanged in the experimental buffer using NAP-5 Columns (GE Healthcare). Experiments typically involved titrating 25 µM of protein in the sample cell with 300 µM of compound in the syringe ...
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bioRxiv - Immunology 2021Quote: ... buffer-exchanged with DPBS buffer (pH 7) and purified over Superdex 200 Increase 10/300 GL column (GE Healthcare) in DPBS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer, 0.150 M NaCl) using PD-10 desalting columns (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer, 0.150 M NaCl) using PD-10 desalting columns (GE Healthcare). Purity of the proteins were evaluated using SDS-PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Microbiology 2019Quote: ... PCR products were visualized on agarose gel then purified using PCR DNA and Gel Band Purification kit (GE Healthcare, UK).
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bioRxiv - Microbiology 2019Quote: ... then immediately rebuffered in 3.5 mL assay buffer (sodium citrate buffer 50 mM, pH 6) in a PD-10 gel filtration column (GE Healthcare) and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius) ...
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bioRxiv - Biophysics 2020Quote: ... α-Syn fibrils were formed by buffer exchange of purified monomers into fibril forming buffer (20mM Sodium phosphate, pH7.5) using a PD-10 column (GE Healthcare). The resulting α-Syn solution was passed through a 0.2 µm syringe filter ...
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bioRxiv - Biophysics 2022Quote: ... The excess Cy3-NHS was removed and the buffer was exchanged with a 20 mM MES buffer at pH 5.5 using a PD-10 column (GE Healthcare). The typical labeling efficiency ...
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bioRxiv - Synthetic Biology 2019Quote: ... and the buffer was replaced with storage buffer (100 mM phosphate buffer, 10% glycerol, pH 7.6) via the PD-10 column (GE Healthcare). Protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 ml of 20 µM purified Sup35NM were buffer exchanged into Fibril Forming Buffer (FFB - 20 mM sodium phosphate buffer pH 7.4, 50 mM NaCl) using a PD-10 column (GE Healthcare) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... nanobody was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5 ml of base buffer was then applied to PD10 columns to elute the nanobody protein in 0 imidazole buffer ...
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bioRxiv - Biochemistry 2022Quote: ... protein was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5ml of base buffer was then applied to PD10 columns to elute the RECQL5 protein in base buffer ...
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bioRxiv - Cell Biology 2022Quote: ... beads were washed once in lysis buffer and rotated at 4ºC overnight in wash buffer containing 8 U/ml thrombin (GE healthcare) to remove the GST tag ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were eluted with 500 mM imidazole followed by buffer exchange to buffer (50mM Tris, 300 mM NaCl, pH 8.0) with HiTrap 26/10 Desalting column (GE Healthcare). To obtain protein without sumo tag ...
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bioRxiv - Biochemistry 2023Quote: ... in SEC buffer (20 mM Sodium-phosphate buffer pH 6.5, 50 mM NaCl) using an ÄKTA pure chromatography system (GE Healthcare). Eluted fractions were concentrated using Amicon® Ultra to 1 mM final concentration.
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bioRxiv - Plant Biology 2019Quote: ... using HBS-EP buffer (GE Healthcare Life Sciences) and a Biacore T200 ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were cleaned with an Illustra GFX PCR DNA and gel band purification kit (GE Healthcare, Chicago, IL, USA) and sequenced by using Sanger technology in an ABI 3730XL sequencer (STAB-VIDA ...
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bioRxiv - Plant Biology 2021Quote: ... nested PCR was performed using primers listed in Supporting Information Table S1 and Ready-ToGo beads (illustra PuReTaq PCR Beads, GE Healthcare). In the first round ...
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bioRxiv - Microbiology 2022Quote: ... DNA from PCRs and agarose gels was purified using the Illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). Polymerases ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The PCR product was gel purified by Illustra GFX PCR DNA and Gel Band Purification Kits (GE Healthcare, Chicago, Illinois, USA), cloned into pJET 1.2/blunt vector (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... cysteine variants of α-synuclein were buffer exchanged into PBS or sodium phosphate buffer by use of P10 desalting columns packed with Sephadex G25 matrix (GE Healthcare). The protein was then incubated with an excess of Alexa-488 or Alexa-546 dyes with maleimide moieties (Thermofisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions containing target protein was subjected to buffer exchange with buffer A using a HiPrep 26/10 desalting column (GE Healthcare). Next ...
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bioRxiv - Neuroscience 2019Quote: ... The mixture was then purified via reverse Ni-NTA and the buffer was changed to NMR buffer (see above) using either a NAP-5 desalting column (GE Healthcare) or by S200a size exclusion chromatography.
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bioRxiv - Bioengineering 2021Quote: ... pre-equilibrated with five volumes of imidazole binding buffer (PBS, 10 mM imidazole buffer, pH 7.4) using a fast protein liquid chromatography (FPLC) system (AKTA Pure; GE Healthcare systems). The column was washed with twenty bed volumes of binding buffer (PBS ...
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bioRxiv - Microbiology 2021Quote: ... Bound protein was eluted with elution buffer (lysis buffer containing 250 mM imidazole) and loaded onto glutathione Sepharose resin (GE Healthcare) to separate bound NEC from excess His6-SUMO-UL31 ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM BME) with elution buffer B (buffer A with 500mM Imidazole) on a 5mL His-Trap FF Crude Nickel Column (GE Healthcare). Fractions containing Pol θ were pooled and were separated further on a 5mL HiTrap Heparin HP (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions were pooled and buffer-exchanged to sodium carbonate/hydrogen carbonate buffer pH 10 using a Sephadex G-25 column (GE Healthcare, UK). Recombinant protein was stored at −20 °C until required ...