Labshake search
Citations for GE Life Sciences :
351 - 400 of 763 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... The column was washed with 10 column volumes of Buffer A (10 mM HEPES, pH 7.3, 0.15 M NaCl, 5 mM imidazole) on an Aktapurifier (GE Healthcare) while collecting fractions ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and buffer exchanged via a hollow fiber ultrafiltration apparatus (30 kDa Molecular Weight Cut-Off, GE healthcare, Piscataway, NJ) into a buffer containing 10 mM sodium carbonate/bicarbonate (pH 10 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant was loaded onto a column containing sonication buffer pre-equilibrated glutathione-Sepharose 4B beads resin (GE Healthcare). Afterwards ...
-
bioRxiv - Molecular Biology 2023Quote: ... Free dye was removed with buffer exchange to carbonate-bicarbonate buffer (0.2 M NaHCO3 – Na2CO3, pH 8.5) using size-exclusion chromatography (PD-10 Desalting columns, GE Healthcare). Next ...
-
bioRxiv - Bioengineering 2023Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
-
bioRxiv - Biophysics 2023Quote: ... Buffer conductivity was measured on the built-in conductivity monitor of the chromatographic system AKTA Pure 150M (GE Healthcare). The sample was kept at 4 °C no more than 7 d ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 55 cm Hg for 1.5 hrs with RNA transfer buffer (3 M NaCl, 0.01 N NaOH) using a VacuGene XL (GE Healthcare). The membrane was rinsed with 2x SSC for 10 min ...
-
bioRxiv - Biochemistry 2023Quote: ... 30 mM NaCl (buffer A) the supernatant was loaded onto a Mono S cation exchange chromatography column (GE Healthcare) equilibrated with buffer A using a ÄKTA Pure system (GE Healthcare) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed with 3X with TBST buffer and incubated with appropriate HRP-conjugated secondary antibodies (GE Healthcare) for 1 hour at RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.5 N NaOH) and 2 h with transfer buffer (1.5 M NaCl, 0.25 N NaOH) using a VacuGene XL (GE Healthcare). The DNA was fixed to the membrane by soaking it in freshly prepared 0.4 N NaOH for 10 min ...
-
bioRxiv - Biochemistry 2023Quote: ... The buffer was exchanged in assay buffer (10 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.1 % Tween-20) using PD10 gravity columns (GE Healthcare). The protein was concentrated using Amicon Ultra-0.5 10K Centrifugal Filter Devices (Merck Millipore) ...
-
bioRxiv - Biophysics 2023Quote: ... The TSP was then circulated through a pre-equilibrated (buffer A) Ni-NTA column (HisTrap, 5 ml, GE HealthCare) for 4 h at 4 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... 5 mM 2-mercaptoethanol and desalted into the same buffer using a 5 mL HiTrap desalting column (GE Healthcare) using an AKTA FPLC system (Cytiva ...
-
bioRxiv - Genetics 2020Quote: ... Radiolabeled probes were prepared by random priming of PCR products covering the regions of interest with Megaprime kit (GE Healthcare) in the presence of α-32P dCTP (3000 Ci/mmol) ...
-
bioRxiv - Biochemistry 2019Quote: ... The template strand was purified by binding the biotinylated PCR product to Streptavidin Sepharose High Performance affinity resin (GE Healthcare), purification of the beads from the reaction mixture by repeated washing with Tris buffer (10 mM Tris HCl pH 7.5 ...
-
bioRxiv - Genetics 2019Quote: ... 20 μg of PCR products was immobilized to 5 μl Streptavidin Sepharose High Performance Bead (GE Healthcare, Piscataway, NJ, USA), and subsequently annealed to 1 μl sequencing primer (5 μM ...
-
bioRxiv - Genomics 2020Quote: ... 20 µl PCR products were subsequently immobilized to 5 µl Streptavidin Sepharose High Performance Bead (GE Healthcare, Piscataway, NJ, USA), and then were finally annealed to 1 µl sequencing primer (5 μM ...
-
bioRxiv - Microbiology 2021Quote: ... The ligated DNA templates were cleaned by an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, U.S.A). Finally ...
-
bioRxiv - Plant Biology 2020Quote: ... The coding region of PUB25 was PCR-amplified from previously described pET28a:PUB25 vectors (49) and cloned into bacterial expression vector pMAL-c2x (GE Healthcare) by digestion and ligation cloning using XbaI and PstI-HF (NEB ...
-
bioRxiv - Microbiology 2021Quote: ... Digestion products were purified with the illustra GFX PCR DNA and gel band purification kit (GE Healthcare, Little Chalfont, UK) and were directionally cloned in the yeast expression vector pYES2 (Invitrogen ...
-
bioRxiv - Genetics 2019Quote: ... PCR amplicons from each species presenting positive signals for Micropia were separated by 1.5% agarose gel electrophoresis and purified using Illustra GFXTM PCR DNA and Gel Band Purification kit (GE Healthcare) according to the supplier’s specifications ...
-
bioRxiv - Plant Biology 2023Quote: ... first-strand cDNA was synthesized from 2 µg of total RNA using Ready-to-Go RT-PCR beads (GE healthcare) and an oligo(dT ...
-
bioRxiv - Biochemistry 2024Quote: ... The amplified PCR product was digested with BamHI and XhoI and ligated into the vector PGEx-6p-1 (GE Healthcare) digested with the same restriction enzymes ...
-
bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
-
bioRxiv - Biophysics 2021Quote: ... followed by buffer exchange in (50 mM HEPES, 150mM NaCl, pH 7.5) using a Superdex peptide size-exclusion column (GE Healthcare) to give the fluorescence labeled degron and Ub-degron.
-
bioRxiv - Biophysics 2021Quote: ... The volume 100 μl of 200-500 μM protein samples in PBS buffer (pH 7.4) after Ni purification was injected into a Superdex 75 Increase 10/300 GL column (GE Healthcare) or a Shodex KW-802.5 (Showa Denko K.K. ...
-
bioRxiv - Cell Biology 2020Quote: Fresh embryo protein extracts (4 mg in 2.5 ml RIPA buffer) was passed through PD-10 desalting column (GE Healthcare) to remove excess free biotin using the gravity protocol according to manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2020Quote: ... the buffer of the DNA samples was exchanged to SDAD cross-linking buffer (100 mM NaHCO3 pH 8.3) using MicroSpin S400 columns (GE Healthcare). 1 μg of dsDNA was incubated with 2 mM SDAD cross-linker in 25 μl of SDAD cross-linking buffer at 25 °C overnight ...
-
bioRxiv - Cell Biology 2019Quote: ... elutes of KLP10A were subjected to buffer exchange to 1xMRB80 with 75 mM KCl and 0.1 mM ATP using a PD-25 column (GE Healthcare). AtMIDD1 was purified following (Oda and Fukuda ...
-
bioRxiv - Cell Biology 2019Quote: ... Buffer was then exchanged to 1xSAB supplemented with 1 mM ATP to remove imidazole using PD-25 column (GE Healthcare).
-
bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... was exchanged into labelling buffer (20 mM Tris-HCl pH 7.5, 100 mM NaCl) using size exclusion NAP10 columns (GE Healthcare). Protein-containing fractions were identified by protein electrophoresis and pooled ...
-
bioRxiv - Microbiology 2019Quote: ... Purified protein was immediately exchanged into TEV cleavage buffer (50 mM Tris-HCl pH 8.0, 10% glycerol) using a 50 ml desalting column (GE Healthcare). For His-tag cleavage ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein was then loaded onto a transport buffer equilibrated Superdex 75 gel filtration column (GE Healthcare, #28-9893-33) and fractions containing the protein of interest were pooled ...
-
bioRxiv - Molecular Biology 2020Quote: ... was pre-equilibrated for 1.5 hrs at 4°C with Sup6-150 buffer without inhibitors or DTT on an AKTA Purifier system (GE Healthcare). 500 μL lysate (2 mg protein ...
-
bioRxiv - Biophysics 2022Quote: ... Eluted Sox2 was refolded by changing to a zero-urea buffer using a desalting column (GE healthcare #17-1408-01). Further purification was performed by gel filtration on a Superdex 200 10/300 GL column (GE Healthcare) ...
-
bioRxiv - Biophysics 2022Quote: ... 25 μL of this mixture was then chromatographed at room temperature and a flow rate of 0.04 mL/min on a Superdex-200 3.2/300 size exclusion column equilibrated in assembly buffer (GE Healthcare Ettan). A 50 μL fraction containing the largest molecular weight complex was assessed by SDS-PAGE (stained with SYPRO Red [Thermo Fisher] ...
-
bioRxiv - Biochemistry 2019Quote: ... and buffer exchanged to 10 mM phosphate buffer pH 7.4 containing 10 mM MgCl2 using 10 kD Vivaspin 20 ml columns (GE Healthcare). Samples were then loaded onto a CHT ceramic hydroxyapatite column (Bio-Rad Laboratories Inc.) ...
-
bioRxiv - Biochemistry 2020Quote: ... and the buffer was exchanged to 50 mM HEPES pH 7.0 containing 150 mM NaCl and 10 mM CaCl2 (Reaction Buffer) using a PD-10 desalting column (GE Healthcare). Aliquots containing 240 µg total protein were incubated at 37 C in the presence of 750 nM MMP2 or 9 or the Reaction Buffer alone for 2 hours ...
-
bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... The solution was dialyzed against Buffer C overnight at 4 °C and applied on a Resource Q column (GE Healthcare) that was equilibrated with Buffer C ...
-
bioRxiv - Biochemistry 2021Quote: ... The periplasmic extract was adjusted to 50 mM Tris/HCl pH 9.0 (loading buffer) and loaded onto a 5 mL Q Sepharose anion exchange column (GE Healthcare). Proteins were eluted using a linear salt gradient (0-500 mM NaCl ...
-
bioRxiv - Biochemistry 2020Quote: ... The eluted protein sample was dialyzed against 20 mM Tris-HCl (pH 8.0) and 2 mM CaCl2 (buffer B) and applied to a Mono Q 10/100 GL column (GE Healthcare) equilibrated with buffer B ...
-
bioRxiv - Biochemistry 2020Quote: The sample was desalted into Buffer A using a HiPrep 53 ml 26/10 desalting column (GE Healthcare, Chicago, IL) with 14 ml injections at 9 ml/min for all steps ...
-
bioRxiv - Molecular Biology 2020Quote: ... the purified proteins were dialyzed against TN200 buffer (20 mM Tris-HCl pH 7.5, 200 mM NaCl) using a HiTrap Dessalting column (GE Healthcare), and the protein was further purified by gel filtration chromatography on a HiLoad 16/600 Superdex 75 or HiLoad 16/600 Superdex 200 column (GE Healthcare ...
-
bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
-
bioRxiv - Biochemistry 2021Quote: ... Purified Ycf1p NBD1 and NBD1-R were exchanged into the fluorescence buffer (20 mM Na3PO4, 2% glycerol, 150 mM NaCl, pH 7.3) by size exclusion chromatography (Superdex75, GE Healthcare). MgATP-bound and apo NBD1 and NBD1-R were generated by adding 2 mM MgATP or 2 mM EDTA ...
-
bioRxiv - Cell Biology 2021Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
-
bioRxiv - Plant Biology 2021Quote: ... in running buffer (20 mM Tris, pH 8, 150 mM NaCl, 1 mM DTT) supplemented with 0.05% of P20 surfactant (GE Healthcare). Anti-MBP murin monoclonal antibody (Biolabs ...
-
bioRxiv - Microbiology 2020Quote: ... and eluted in superdex buffer (20 mM Tris [pH 8.0], 300 mM NaCl, 1 mM TCEP) using the AKTA Explorer FPLC system (GE Healthcare). The protein was concentrated to 7.5 mg/ml.