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Citations for GE Life Sciences :
201 - 250 of 994 citations for Histone H4 mono methyl K16 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The sample was diluted 3-fold with dilution buffer (50 mM Tris pH 7.5, 10 mM β-mercaptoethanol) before loading onto a MonoS 5/50 GL column (GE Healthcare) with buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then diluted 10 times with mono Q binding buffer (50 mM HEPES, pH 8.0) and applied to a MonoQ 5/50 GL column (GE Life Sciences) connected to an AKTA Pure system (GE Life Sciences). ...
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bioRxiv - Biophysics 2020Quote: ... Cleaved protein was eluted from the beads and further purified on a cation exchange column (Mono S 10/100, GE Healthcare) in MES pH 6.5 ...
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bioRxiv - Immunology 2020Quote: ... FP3 or FP4 from the Mono S column were pooled and further purified by SEC performed on a 24 mL Superdex 200 increase column (GE Healthcare) at 4 °C with a flow rate of 0.25 mL/min in a buffer containing 20 mM HEPES ...
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bioRxiv - Immunology 2020Quote: ... Samples containing activity were fractionated by anion exchange chromatography on a Mono Q 5/200 GL column (GE Healthcare, Piscataway NJ) using a gradient from 50mM Tris-HCl (pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved MBP tag and TEV protease were removed by anion exchange chromatography using a Mono Q 5/50GL (GE Healthcare) column pre-equilibrated with buffer-B ...
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bioRxiv - Molecular Biology 2022Quote: ... The purified MUS81-EME1 was subjected to cation-exchange chromatography (CEC) with a Mono S 4.6/100 PE column (GE Life Sciences) on ÄKTA explorer FPLC system ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was clarified by centrifugation at 13000 rpm for 10 min at 4°C by a table-top centrifuge and further purified by chromatography on a Mono Q HR 5/5 column (GE Healthcare) with a linear gradient of 0.1-1.0 M sodium chloride in chromatography buffer (20 mM HEPES–KOH pH 7.6 ...
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bioRxiv - Biochemistry 2023Quote: ... and ion exchange of the excised fragment by HiTrap DEAE (147-bp ‘601’, 185-bp ‘601’, and MMTV; Cytiva) or mono-Q (NLE- trimer ‘601’; GE Healthcare) according to established protocols(Dyer et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The fractions collected at the elution peak were pooled together and loaded into a MonoS 5/50 GL column (GE Healthcare) previously equilibrated with IEX Equilibration buffer (50 mM HEPES pH7.8 ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Microbiology 2021Quote: ... and SARS2-38 mAb proteins were produced in CHOEXPI or EXPI293F cells and affinity purified using HiTrap Protein A columns (GE Healthcare, HiTrap mAb select Xtra #28-4082-61). Purified mAbs were suspended into 20 mM histidine ...
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bioRxiv - Immunology 2020Quote: ... The FITC labelled mAb were purified using a desalting column (GE Healthcare). Cetuximab was a kind gift from Thomas Valerius and Campath-1 was a kind gift from Geoff Hale.
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bioRxiv - Biochemistry 2021Quote: ... The fractions near the maximum height of the peak were combined and further purified by a Mono Q 10/100 GL column (GE Healthcare, USA). Buffer exchange to buffer E ...
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bioRxiv - Molecular Biology 2021Quote: ... Histones were then purified further by passing over a HiTrap Q column (GE Healthcare) before binding to and NaCl gradient elution from HiTrap SP cation exchange chromatography columns (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting histone H2A was further purified by HiTrap SP column chromatography (GE Healthcare), flash-frozen with LN2 ...
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bioRxiv - Biochemistry 2020Quote: ... Excess histones and enzymes were removed using a S-400 spin column (GE Healthcare). ATPase reactions were initiated by adding an ATP/MgCl2 Mix (final concentration 2.5 mM each) ...
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bioRxiv - Cell Biology 2020Quote: ... Each oligonucleotide probe contained 4 primary amines that were conjugated to Cy3 using the Mono-Reactive Dye Pack (PA23001, GE Healthcare Life Sciences). To this end ...
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bioRxiv - Cell Biology 2020Quote: ... Each oligonucleotide probe contained 4 primary amines that were conjugated to Cy3 using the Mono-Reactive Dye Pack (PA23001, GE Healthcare Life Sciences). To this end ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was purified away from SenP1 and the N-His6-SUMO-tag by FPLC on a Mono Q 10/100 GL anion exchange column (GE Healthcare Life Sciences) using buffers containing 50 mM EPPS (pH 8.5) ...
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bioRxiv - Immunology 2020Quote: ... Fractions that yielded proliferation of TN T cells were pooled and subjected to cation exchange chromatography on a Mono S 5/200 GL column (GE Healthcare, Piscataway NJ). The active fractions were again pooled and subjected to buffer exchange (Amicon ultra 3k cutoff ...
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bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
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bioRxiv - Neuroscience 2022Quote: ... The stabilized Q-SNARE complex was purified by Ni2+-NTA affinity chromatography followed by ion-exchange chromatography on a Mono Q column (GE Healthcare, Piscataway, NJ) in the presence of 50 mM n-octyl-β-D-glucoside (OG)10 ...
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bioRxiv - Immunology 2023Quote: ... The products were filtered by 10 kDa-cutoff Amicon Ultra centrifugal filters to remove DNA and proteins and then analyzed by a Mono Q 5/50 GL column (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2023Quote: ... The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences). Finally ...
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bioRxiv - Biophysics 2020Quote: ... Trastuzumab and deglycosylated trastuzumab mAb were immobilized on a CM5 chip (GE Healthcare) by amine coupling ...
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bioRxiv - Bioengineering 2021Quote: ... The eluted mAbs were further purified using equilibrated Capto Q columns (GE Healthcare) and flow-through fractions ...
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bioRxiv - Cell Biology 2020Quote: ... The radiolabelled histone H1 was then detected using a BAS Storage Phosphor screen (GE Healthcare) and read out using a Phosphorimager Typhoon 8600 (Molecular Devices).
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bioRxiv - Molecular Biology 2020Quote: ... 4.5 µL of Cy5 Mono-Reactive NHS Ester Fluorescent Dye (ChIP samples) or Cy3 Mono-Reactive NHS Ester Fluorescent Dye (control samples Input DNA or no-tag ChIP) (GE Healthcare, PA25001 and PA23001) were added ...
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bioRxiv - Plant Biology 2023Quote: ... Active fractions were combined and exchanged into buffer B (50 mM Tris-HCl, pH 7.5) before being applied to a Mono Q™ HR 5/5 column (GE Healthcare, Piscataway, NJ, USA) equilibrated with the same buffer ...
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bioRxiv - Biophysics 2023Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM βME) and eluted with 0-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... Two mg of proteins in buffer were mixed with 50ul 7-Methyl-GTP-Sepharose 4B (GE Healthcare) previously equilibrated with extraction buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mAbs were purified using HiTrap Protein A HP column (GE Healthcare, Chicago, IL), followed by size exclusion chromatography (Superdex 200 16/90 ...
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bioRxiv - Immunology 2019Quote: ... MAbs were purified over Protein G Sepharose 4 Fast Flow resin (GE Healthcare; 17061802). The antibodies were buffer exchanged into PBS pH 7.4 prior to use in any cell based assays.
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bioRxiv - Microbiology 2022Quote: ... The mAb was purified using five milliliter HiTrap Protein G HP columns (GE Healthcare) equilibrated in 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Histone octamers were purified by size exclusion chromatography (HILoad 16/600 Superdex 200 column, GE Healthcare) and stored at −20 °C in 50 % glycerol.
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bioRxiv - Biophysics 2020Quote: ... The histone octamers were purified by size-exclusion chromatography on a Superdex 200 column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Histone octamers were purified by size exclusion chromatography (HILoad 16/600 Superdex 200 column, GE Healthcare) and stored at −20 °C in 50 % glycerol.
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bioRxiv - Biophysics 2022Quote: The N-terminal 6x His tags were removed from the histones by thrombin protease (GE Healthcare) treatments using 1 unit per 1 mg of protein for 3-5h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... histones were purified on a gel filtration column HiLoad Superdex 75 pg (GE Healthcare Life Sciences) pre-equilibrated with histone gel-filtration buffer (HGFB ...
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bioRxiv - Molecular Biology 2023Quote: ... Histone octamer was purified by Superdex 200 Increase 10/300 GL column-based SEC (GE Healthcare) that was pre-equilibrated with refolding buffer ...
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bioRxiv - Biophysics 2020Quote: ... thrombin cleavage of the His6-tag overnight and final ion exchange chromatography with a MonoQ or MonoS column (Äkta purifying system, GE Healthcare, Little Chalfont, United Kingdom). Synaptobrevin 2 49-96 S79C was labeled with Atto488 maleimide ...
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bioRxiv - Biochemistry 2021Quote: ... DrrA-OCRL or DrrA-INPP5E were then loaded onto a 1 mL MonoS (5/50 GL) cation exchange column (GE Healthcare, Cat# 17-5168-01) equilibrated in 20 mM HEPES pH 7 ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant proteins were purified using a HIS-Trap (GE Healthcare) or Amylose resin (New England Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... 12 μl of pre-blocked m7G affinity beads (50 % slurry, 7-Methyl-GTP Sepharose® 4B, GE Healthcare) and 200 μl of BB-250 (250 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... the mAb was purified using a 1 mL HiTrap Protein A HP column (GE Healthcare). The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... mAb followed by horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2,000, NXA931, GE Healthcare). Periplasmic fractions ...
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bioRxiv - Cancer Biology 2019Quote: ... or anti-phospho hTERT mouse mAb (TpMab-1) and anti-mouse IgG HRP (GE Healthcare) were used for IB to detect phosphorylated hTERT proteins.
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified from serum-free culture supernatants using mAb Select SuRe resin (GE Healthcare) followed by rapid desalting using a HiPrep 26/10 Desalting column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... Purification from serum-free culture supernatants was done using mAb Select SuRe resin (GE Healthcare) followed by rapid desalting using a HiPrep 26/10 Desalting column (GE Healthcare) ...