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Citations for GE Life Sciences :
151 - 200 of 994 citations for Histone H4 mono methyl K16 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the secondary mAB anti-mouse (sheep, GE Healthcare, LNA931V) and developed using Clarity Western ECL Substrate (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... the immobilized proteins were eluted and further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were assessed by SDS–PAGE followed by Coomassie blue staining ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted out and further purified by a mono Q 5/5 column (GE Healthcare) and a Superose S6 Increase 5/150 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP and Input samples were labeled with mono-reactive NHS ester fluorescent Cy5 and Cy3 dyes (GE Healthcare) respectively ...
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bioRxiv - Bioengineering 2022Quote: ... Further purification was performed by ion-exchange chromatography using mono Q 5/50 GL (GE HealthCare, Uppsala, Sweden) for each fusion protein in 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The combined eluate was then subjected to cation- exchange chromatography using a MonoS 10/100 column (GE healthcare) and eluted with a linear gradient of NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... mTLR9-ECD was further purified by ion-exchange chromatography with a monoS 4.6/100 PE column (GE Healthcare) in 10 mM MES pH 6.0 ...
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bioRxiv - Microbiology 2020Quote: ... cleavage products from Ulp protease cleavage were separated by additional ion-exchange column (GE healthcare, MonoS G5/50) using a KCl gradient of 0.1M to 1M in MonoS-Sucrose buffer (20% sucrose ...
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bioRxiv - Genetics 2020Quote: ... H31-24Ub2 and H31-24K9me3Ub2 were achieved through cation-exchange chromatography on a mono S column (GE Healthcare).
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bioRxiv - Neuroscience 2024Quote: ... diluted twice to lower salt concentration to 50 mM and bound to a MonoS Sepharose column (GE Healthcare). Finally ...
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bioRxiv - Biophysics 2022Quote: ... each histone was subjected to Resource S column chromatography (GE Healthcare). The column was washed with buffer D (20 mM sodium acetate (pH 5.2) ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified on an ion exchange column MonoS and gel filtration column Superose 6 (GE Healthcare). Purified protein was concentrated to ∼20 mg/ml in BRB20 buffer (20mM PIPES ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was additionally purified by ion-exchange chromatography on a “Mono S 5/50 GL” column (GE healthcare) at 0-100% B gradient over 15 column volumes at 0.5 ml/min flow rate using 20 mM sodium phosphate buffer (pH 6.0 at 25 °C ...
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bioRxiv - Microbiology 2019Quote: LecB was labeled with fluorescent dyes bearing NHS esters as reactive groups (Cy3 mono-reactive NHS ester (GE Healthcare), Cy5 mono-reactive NHS ester (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... Samples were injected onto a Mono-Q column on AKTA fast performance liquid chromatography instrument (GE Healthcare Life Sciences) and eluted in 20 mM Tris⋅HCl (pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... pH 4.5) and K29-linked and K48-linked chains were separated by ion exchange (MonoS cation column, GE Healthcare) with buffer A containing 50 mM NaOAc ...
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bioRxiv - Biochemistry 2023Quote: ... 30 mM NaCl (buffer A) the supernatant was loaded onto a Mono S cation exchange chromatography column (GE Healthcare) equilibrated with buffer A using a ÄKTA Pure system (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting sNASPc–H3–H4–ASF1b heterotetramer was first purified by Ni Sepharose 6 Fast Flow beads (GE Healthcare). After removal of the His6-SUMO tag by home-made Ulp1 (SUMO protease) ...
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bioRxiv - Biophysics 2019Quote: ... The mutant histone was purified and labeled with Cy3 maleimide (GE Healthcare) under denaturing conditions (Harada et al. ...
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bioRxiv - Biophysics 2022Quote: ... The RNA samples were first purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare, Chicago, IL) at pH 7.0 then annealed in buffers containing 40 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by on-column digestion and the eluate was further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
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bioRxiv - Synthetic Biology 2020Quote: ... the pH adjusted to 5.0 with 1 M HCl and loaded on a Mono S column HR10/30 (GE Healthcare), equilibrated with 25 mM acetate buffer ...
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bioRxiv - Immunology 2021Quote: ... TCR and pMHC proteins were dialyzed and further purified via anion exchange chromatography (HiTrap Q HP; Mono Q; GE Healthcare) and size-exclusion (Superdex 200 ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein sample was dialyzed against 20 mM Tris-HCl (pH 8.0) and 2 mM CaCl2 (buffer B) and applied to a Mono Q 10/100 GL column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Cell Biology 2019Quote: ... the coating was done with a 2:1 ratio of non-coupled:Cy3-coupled fibronectin prepared with Cy3 Mono-Reactive Dye Pack (GE Healthcare) as recommended by the manufacturer.
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bioRxiv - Biophysics 2019Quote: ... diluted such that the NaCl concentration was < 50 mM and applied to a Mono Q 10/100 GL (GE Healthcare) equilibrated in 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.5) for subsequent anion exchange chromatography on a 5 ml Mono-Q column (GE Healthcare Europe GmbH, Freiburg, Germany). Following an elution gradient with high-salt buffer (20mM TRIS ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM EDTA and 1 mM DTT) and loaded onto a Mono Q 10/100 GL column (GE Healthcare, Inc.) equilibrated in buffer E and eluted with a 160 mL linear gradient of 0.3–0.5 M NaCl in buffer E ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved protein was diluted further to 0.2M NaCl concentration and applied to cation exchange 10/10 Mono S column (GE Healthcare). Small terminase was eluted in two peaks with 0.2- 1M NaCl gradient ...
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... the sample was filtered and applied to an equilibrated ion-exchange chromatography column (Mono S 10/100 GL, GE Healthcare), and weakly bound proteins were washed out with buffer A ...
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bioRxiv - Biophysics 2023Quote: ... the sample was filtered and applied to an equilibrated ion-exchange chromatography column (Mono S 10/100 GL, GE Healthcare), and weakly bound proteins were washed out with buffer A ...
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bioRxiv - Cancer Biology 2023Quote: ... WCLs were incubated with 50 μl 7-methyl-GTP Sepharose 4B beads (GE Healthcare) for 2 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Secreted mAbs were purified using a Protein G column (GE Healthcare) and concentrated for use in downstream assays.
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bioRxiv - Immunology 2022Quote: ... Secreted mAbs were purified using a Protein G column (GE Healthcare) and concentrated for use in downstream assays.
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bioRxiv - Microbiology 2021Quote: ... Cy3-labelled goat-anti-mouse mAb (PA43009, GE Healthcare, 1:3,000) was subsequently used as a secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... mAbs were purified using protein G sepharose resin (GE Life Sciences) and buffer-exchanged into D-PBS using Zeba 96-well desalting plates (Thermo-Fisher Scientific).
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bioRxiv - Biophysics 2021Quote: ... thrombin was removed and the proteins were concentrated by ion exchange chromatography on MonoQ or MonoS columns (Äkta purifying system, GE Healthcare). The Δ49-complex was assembled by first mixing syx-1 and the soluble syb 2 fragment (1:2 ...
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bioRxiv - Biochemistry 2020Quote: ... and 5% (v/v) glycerol for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 50 mM to 1 M NaCl ...
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bioRxiv - Biochemistry 2019Quote: The rate of alarmone synthesis was determined by quantifying AMP levels at set time-points using anion exchange chromatography (on a 1 ml Mono Q 5/50 GL column (GE Healthcare), as described above) ...
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bioRxiv - Biochemistry 2020Quote: ... The eluate was diluted two-fold in buffer E + 1 mM DTT + 150 mM NaCl and applied to a MonoS column (GE Healthcare) pre-equilibrated in buffer E + 150 mM NaCl + 1 mM DTT + 0.5 mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... It was first purified by a glutathione-affinity step and then subsequent to tag removal following incubation with 3C protease further purified on a MonoS ion-exchange (GE Healthcare) column equilibrated into 25 mM MES pH 6.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleaved KIX was purified using a linear gradient of 50 mM to 1 M NaCl over ten column volumes by cation exchange chromatography using MonoS 5/50 GL column at a flow rate of 1 mL per minute (GE Healthcare). Fractions containing purified KIX were confirmed by SDS-PAGE and dialyzed against 1 L of 50 mM potassium phosphate pH 5.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was next loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was then loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... HSP40 fractions containing purified protein were pooled and further purified via anion exchange using a Mono Q anion exchange chromatography column (GE Healthcare) using an AKTAExplorer 100 FPLC (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto either a 1-ml Mono Q (ParAF proteins and ParBF1-42-mCherry-EcoRIE111Q) or Mono S (other ParBF proteins) 5/50 GL ion exchange column (GE Healthcare) pre-equilibrated with Mono Q/S-buffer containing a NaCl concentration to match the conductivity of the sample ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescently labeling of Pol β followed the same procedure using an Amersham Cy5 mono-reactive NHS ester dye pack (GE healthcare). Labeling efficiency was determined by calculating the molar ratio of dye-to-protein within the solution by measuring absorbance at 280 nm and 530 nm for APE1-Cy3 or 649 nm for Pol β-Cy5 ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was diluted 5-fold with buffer A (50 mM Tris pH 7.5, 10 mM β-mercaptoethanol) before loading onto a MonoS 5/50 GL column (GE Healthcare) with buffer A supplemented with 100 mM NaCl ...