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Citations for GE Life Sciences :
151 - 200 of 1133 citations for CTLA 4 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... goat polyclonal anti-GST (GE healthcare, 27-4577-01; 1:2000, IB); rabbit polyclonal anti-Cap1 [1 μg/ml ...
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bioRxiv - Plant Biology 2020Quote: ... GST-tagged proteins were purified using Glutathione Sepharose 4B (GE Healthcare, USA). Protein concentration was determined using Bio-Rad protein assay (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... GST-fused proteins were purified using glutathione 9epharose 4B beads (GE HealthCare) equilibrated in 1x Phosphate Buffered Saline (PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were incubated with 250 μL of GST Sepharose 4B (GE Healthcare) for 3 hours at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... coli GST sequence on the pGEX-2T plasmid (GE Healthcare, Freiburg, Germany) using BamHI and EcoRI restriction sites ...
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bioRxiv - Cell Biology 2020Quote: ... The soluble fraction was bound to the GST Sepharose 4B (GE Healthcare) for 90 to 120 min at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... GST-fusion proteins were purified using glutathione-Sepharose 4B columns (GE Healthcare). Protein-bound glutathione beads were thoroughly washed with cold PBS six times ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was purified on GST Sepharose 4B resin (GE healthcare) and then eluted in the lysis buffer with 25mM glutathione ...
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bioRxiv - Plant Biology 2022Quote: ... The lysate was incubated with GST beads (Glutathione Sepharose 4B, GE Healthcare) for 2 hours at 4° C ...
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bioRxiv - Cell Biology 2022Quote: ... Affinity purification of GST-SseK3 was performed using glutathione sepharose (GE Healthcare), and purification of 6×His-SUMO-hUBE1 ...
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bioRxiv - Developmental Biology 2022Quote: ... GST fusion proteins were affinity purified over Gluthione Sepharose 4B (GE Healthcare). After extensive washing ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells were passaged in HyClone DMEM (GE Healthcare Systems, Marlborough, MA) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cell lysates were incubated with 20 µL IgG Sepharose (GE Healthcare) or as a control Sepharose without IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... freshly purified GST-RILP protein bound to GSH-Sepharose 4B beads (GE Healthcare) was incubated with extracts from transfected HEK293T cells (one 10-cm diameter dish per assay ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins were pull-down with glutathione Sepharose 4B beads (GE Healthcare), pre-washed with ice-cold PBS ...
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bioRxiv - Physiology 2019Quote: ... GST-tagged protein from supernatant were purified using glutathione-Sepharose 4B (GE Healthcare). Sepharose bound proteins were used as the protein columns ...
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bioRxiv - Biochemistry 2020Quote: ... Glutathione sepharose 4B GST beads was purchased from GE Healthcare (Mississauga, ON, Canada). Lipofectamine 2000 and Alexa Fluor 488 goat anti-mouse IgG were purchased from Invitrogen (Burlington ...
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bioRxiv - Cell Biology 2020Quote: ... GST tag was removed by incubating with 2 units PreScission protease (GE Healthcare) per 100 μg protein for 16-18 h at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... GST tagged proteins were purified with Glutathione Sepharose 4B beads (10250335, GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The GST fusion proteins were purified with Glutathione Sepharose ™4B (GE Healthcare) according to the manufacturer’s protocol with Binding Buffer I [50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... GST tags were cleaved with PreScission protease per the manufacturer’s instructions (GE Healthcare). Isolated CFP1 fragments were dialyzed in 25% glycerol ...
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bioRxiv - Plant Biology 2019Quote: ... The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare) was incubated with the Arp2/3 complex in pull-down buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Primary antibodies were directed against GST (GE Healthcare Cat# 27-4577-01, RRID:AB_771432), murine AOC3 (Abcam Cat# ab42885 ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged proteins were immobilized on glutathione sepharose beads (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell lysates were precleared with GST immobilized on glutathione–Sepharose 4B (GE Healthcare) and then incubated with the indicated GST fusion proteins (2–5 μg ...
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bioRxiv - Molecular Biology 2021Quote: ... The GST-proteins were purified using Glutathione Sepharose 4B (GE Healthcare Life Sciences) according to the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The GST-TTD fusion protein was purified using Glutathione sepharose 4B (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was cleaved by the addition of PreScission Protease (GE Healthcare) with rocking incubation overnight at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... GST-β2HA was purified from the soluble fraction using glutathione resin (GE Healthcare). The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission ...
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bioRxiv - Cell Biology 2020Quote: ... the GST-tag was removed by proteolytic cleavage with PreScission Protease (GE Healthcare), followed by a final polishing step of the proteins by size-exclusion chromatography on an Äkta Purifier System using either HiLoad 26/600 Superdex 200 or HiLoad 26/75 Superdex columns (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... GST-fusion proteins were purified by incubation with glutathione-Sepharose beads(GE Healthcare). The precipitates were washed 3X with lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... The recombinant GST fusion proteins were affinity purified on GSH-Sepharose (GE Healthcare) from bacterial cultures incubated with 0.25 mM isopropyl-β-D-thiogalactopyranoside (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... GST fusion proteins were immuno-precipitated using Glutathione Sepharose 4B beads (GE Healthcare). Fusion protein-bound beads were used directly in GST pull-down assays ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... GST-RIG-I protein variants were affinity purified by GSTrap column (GE Healthcare). After sample loading ...
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bioRxiv - Cell Biology 2023Quote: GST-tagged proteins were affinity-purified using glutathione-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s protocols ...
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bioRxiv - Biophysics 2023Quote: ... The clarified supernatants were loaded onto a GST column (GSTrap HP, GE Healthcare) or a nickel affinity chromatography column (HiTrap HP ...
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bioRxiv - Biochemistry 2023Quote: ... All the GST-tagged proteins were bound to GSTrap HP columns (GE Healthcare) and were eluted with 10 mM reduced glutathione ...
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bioRxiv - Plant Biology 2024Quote: ... GST-TZF1 was purified using glutathione-Sepharose 4B resin (GE Healthcare Bio Sciences) as previously described (Qu et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-FBH1[1-250] in the pGEX6P-1 vector (Amersham/GE Healthcare/Cytiva) was introduced into BL21:DE3 Rosetta cells (Novagen) ...
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bioRxiv - Plant Biology 2023Quote: ... GST fusion proteins were purified on Glutathione-Sepharose® 4B beads (GE Healthcare) and eluted using a solution of 50LmM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2021Quote: ... and the GST-fusion protein was purified using Glutathione-Sepharose affinity columns (GE Healthcare). The proteins were finally eluted in PBS containing 20 mM glutathione (pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... GST-OsbZIP86 and MBP-OsSAPK10 were incubated with Glutathione Sepharose 4B (GE Healthcare, USA) in pull-down buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged mammalian ATG8 fusion proteins were cloned into pGEX-4T-1 (GE Healthcare) and expressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Biophysics 2021Quote: ... The GST tagged Gbp2 proteins were purified using glutathione sepharose 4B resin (GE Healthcare) followed by size exclusion chromatography using a Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... and GST-hFMRP KH0-KH1-KH2 were purified using glutathione affinity chromatography (GE Healthcare). All proteins were further purified using Superdex 75 16/60 or Superdex 200 16/60 (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The GST affinity tag was then cleaved using the PreScission Protease (GE Life Sciences) for four hours in cleavage buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: GST-tagged proteins were purified using affinity chromatography with Glutathione Sepharose beads (GE Healthcare) in a cold room ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified over Glutathione (GSH) beads (GE Healthcare Biosciences, Pittsburg, PA) in PBS buffer supplemented with protease inhibitors and eluted with 20mM GSH in PBS pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... The GST-CP was bound to glutathione Sepharose beads (GE Healthcare, 17- 5132-01) for 3 h at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was incubated with 5mL of washed and packed GST resin (GE Healthcare) for 1 hour at 4 °C with gentle rocking ...