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Citations for GE Life Sciences :
1 - 50 of 1133 citations for CTLA 4 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... GST-AtEPL1A and GST-AtEPL1B were purified on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission, GE Healthcare). Fusion protease was removed using glutathione resin and the cleaved β2HA was collected in the flow-through ...
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bioRxiv - Molecular Biology 2023Quote: ... CpxA31-163-GST was purified at 4°C using a protocol modified from the GST Gene Fusion System Handbook (GE Healthcare). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded onto a 4-mL GST column (GE Healthcare) equilibrated in PBS containing 1 mM EDTA ...
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bioRxiv - Cell Biology 2020Quote: Polyclonal anti-GST antibodies (GST Capture Kit, GE Healthcare) were amine coupled on to a Series S CM5 sensor chip (GE Healthcare ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare), washed in lysis buffer and kept on beads in lysis buffer with 1 mM DTT at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified from lysates with glutathione-Sepharose 4 Beads (GE Healthcare). His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated overnight at 4 °C with anti-GST antibody (GE Healthcare) at a 1/1000 dilution ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and cleaved from GST resin overnight at 4°C with PreScission Protease (GE Healthcare). GST was affinity purified as described above and eluted using 15 mM reduced L-gluathione ...
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bioRxiv - Cell Biology 2019Quote: ... GST (GE Healthcare), LC3-II (Cell Signaling) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1μg of GST or GST-Aurora B or GST-ERK was added to GSH beads (GE Healthcare, USA) in binding buffer (50 mM Tris-cl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... His-GST-ARS2 was applied onto a GST resin (GE Healthcare) and His-MBP-ZFC3H1 onto an Amylose resin (NEB) ...
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bioRxiv - Plant Biology 2023Quote: ... GST-OsCOP1 and GST-OsCK2ɑ3 onto GSH- Sepharose (GE Healthcare, USA) by mixing at 4°C for 30 min followed by centrifugation and washing with PBS buffer (Kepinski ...
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bioRxiv - Cell Biology 2022Quote: ... GST-TEAD4-YBD was purified using GST agarose resin (GE Healthcare). GST tag was removed with 3C protease overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: Recombinant GST-NF2 was bound to a GST resin (GE Healthcare), and MBP-NF2 was bound to an MBP resin (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 8.5 μg GST-GST or 8.5 μg GST-BARD1 or 8.5 μg GST-BARD1 + 8.5 μg GST-GST was immobilised on 15 μL Glutathione Sepharose 4B beads (GE Healthcare) in 100 μL pulldown buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... GST-FlgN and GST-FliJ were purified by GST affinity chromatography with a glutathione Sepharose 4B column (GE Healthcare) from the soluble fractions from the Salmonella SJW1368 strain transformed with a pGEX-6p-1-based plasmid as described previously29 ...
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bioRxiv - Biochemistry 2023Quote: ... GST-ICD or ICD-GST (and alanine-scan mutants) and GST-mEGFP were purified through Glutathione Sepharose beads (GE Healthcare), followed by cation exchange chromatography using a Source 15S column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... anti-GST (GE Healthcare), anti-GAPDH (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... GST-FliJ(Δ13–24) and GST-FlgN were purified by GST affinity chromatography using a glutathione Sepharose 4B column (GE Healthcare) as described previously28 ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The GST-MIK1CD is loaded onto GST agarose (Glutathione Sepharose 4B, GE Healthcare) and eluted using GST elution buffer containing 50 mM Tris–HCl ...
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bioRxiv - Plant Biology 2023Quote: ... GST and GST-CTU2 were coupled to Glutathione beads (GE Healthcare Life Sciences) and then were incubated with ROL5-His in the binding buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: Binding affinities were measured using purified and immobilized GST-SH3Boi1 or GST-SH3Boi2 as ligands on an anti-GST chip on a Biacore X100 device (GE Healthcare, Freiburg, Germany). HBSEP buffer was used as running buffer in all experiments ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01). 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega) ...
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bioRxiv - Biophysics 2020Quote: ... The GST was cleaved with GST-tagged HRV 3C protease (PreScission Protease, GE Healthcare). The GST and protease was removed with glutathione sepharose resin ...
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bioRxiv - Biochemistry 2021Quote: ... GST-SUMO2 and GST-diSUMO2 were purified using glutathione sepharose 4B beads (GE Healthcare) according to the manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2023Quote: ... GST or GST-importins were allowed to bind to glutathione-Sepharose 4B (GE Healthcare). In a typical experiment 100 μl beads were pre-equilibrated in IP-buffer (20 mM Hepes pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... and then the GST-YTHDF1 and GST were purified with glutathione beads (GE Healthcare). The resulting purified GST-YTHDF1 or GST was incubated with Myc-STUB1 purified from HEK293T cells ...
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bioRxiv - Biochemistry 2023Quote: ... GST fusion proteins or GST alone were bound on glutathione-Sepharose resin (GE Healthcare) and incubated with 5 μg of purified Snu71 or BBP protein in pull-down buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was incubated for 4 hours at 4°C using a rotating wheel with 3 mL of a GST bead slurry (GE Healthcare Life Sciences) pre-washed 3x with wash buffer (WB ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... goat anti-GST (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2019Quote: ... GST (GE Healthcare, IL, USA), total tau (total human tau ...
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bioRxiv - Cell Biology 2019Quote: ... goat GST (GE healthcare 27457701), rabbit His (Abcam ab9108) ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-GST (GE Healthcare, #27457701V) and anti-goat-HRP (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-GST antibody (GE Healthcare) was used for the detection of GST-tagged bromodomains.
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bioRxiv - Plant Biology 2022Quote: ... The bait protein GST-WL1 was incubated with GST beads (GE Healthcare, Chicago, IL, USA) in non-EDTA buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... GST-SRK2E and GST-SRK2E K50N proteins were expressed using pGEX6p-2 (GE healthcare, IL) and purified using Glutathione Sepharose 4B resin (GE healthcare ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1500 µg protein extract containing GST-Gal1p was loaded on a GST SpinTrap column (GE Healthcare) and incubated for 1.5 hour at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... GST-TRAF6RING and GST-RNF38RING (residues 387-465) were bound to GSTrap HP columns (GE Healthcare) and were eluted with 10 mM reduced glutathione ...
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bioRxiv - Microbiology 2020Quote: ... GST-E1 and GST-PknG were removed from the reactions with glutathione-Sepharose 4B (GE Healthcare). Next ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged expressed proteins were purified using GST beads (Glutathione Sepharose 4B; GE Healthcare Life Science). On the following day ...
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bioRxiv - Cell Biology 2024Quote: ... GST or the GST fusion protein was purified using a glutathione Sepharose 4B column (GE Healthcare). GFP::RAB-5 protein from wild-type or usp-50(xd413 ...
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bioRxiv - Cell Biology 2020Quote: ... GST kit (GE Healthcare, Chicago, IL) was used for purifying GST-Rheb following manufactures instructions and eluted with reduced glutathione ...
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bioRxiv - Developmental Biology 2020Quote: ... or GST Sepharose beads (GE Healthcare). GST pull-down was performed as described previously (Wei et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 25 μL GST beads (GE Healthcare) were added to the system for 2 h on a rotor at 4 °C.