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Citations for GE Life Sciences :
351 - 400 of 1133 citations for CTLA 4 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... The supernatant was transferred to a fresh tube and added with 500 μl of 50 % (vol/vol) GST tagged glutathione-sepharose slurry (GE Healthcare) in lysis buffer and rotated at 4 °C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... The bacteria lysate containing GST-Avr1d was incubated with 10 μl glutathione sepharose 4B beads (Cat: 45-000-139, GE Healthcare) for 3 hours and purified by washing with 1×PBS for 5 times ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was passed over a GST-affinity column (glutathione sepharose™ 4B beads GE Healthcare, Little Chalfont, Buckinghamshire, UK) three times at least ...
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bioRxiv - Cell Biology 2021Quote: Full-length Pak1 constructs (wild type and catalytically inactive) and full-length Sts5 constructs (wild type and phosphomutant) were cloned into pGEX6P1 (GST tag) vector (GE Healthcare) for expression in BL21 DE3 Escherichia coli ...
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bioRxiv - Molecular Biology 2021Quote: ... and expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Cell Biology 2020Quote: ... N571 and N442 in frame with N-terminal tag glutathione S-transferase (GST) in pGEX-6P-3 expression plasmids (GE healthcare) were expressed in BL21-CodonPlus-RP E ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were then passed through Glutathione Sepharose resin to remove the free GST and concentrated to run through Superdex-75 (GE Healthcare) into 200 mM NaCl ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product was cloned downstream and in frame with the glutathione S-transferase (GST) ORF in pGEX-3X (GE Healthcare). The expression construct was transfected into E ...
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bioRxiv - Biochemistry 2022Quote: ... The former vector encodes a glutathione S-transferase (GST) fusion protein that can be removed proteolytically with Prescission Protease (GE Healthcare) after affinity purification ...
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bioRxiv - Molecular Biology 2022Quote: ... Ube2V2 and Ube1 were expressed with N-terminal GST-tag followed by a TEV protease cleavage site and purified via glutathione sepharose resin (GE Healthcare) equilibrated in 50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... 200 pmol of GST-RAC1 and 200 pmol of WAVE complex were mixed with 20 μL of Glutathione Sepharose beads (GE Healthcare) in 1 mL of binding buffer (10 mM HEPES pH 7 ...
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bioRxiv - Biochemistry 2019Quote: ... with expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Cell Biology 2019Quote: ... Human CFP1 and truncation constructs were subcloned into the pGEX-6P-1 vector encoding an N-terminal glutathione S-transferase (GST) tag (GE Healthcare) prior to expression in Origami cells ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Plant Biology 2019Quote: The construct of full length AtMLKL2 (residues 1-711) with N-terminal GST tag was cloned into the pGEX-6P-1 vector (GE Healthcare), and was expressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Biochemistry 2020Quote: A mixture of 1 µM GST-NDP52 and 100 nM GFP-ULK1 complex was incubated with 10 µL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-SV40-NLS were cloned by in-frame insertion of the corresponding NFAT5 fragments and SV40-NLS into the C-terminus of GST of pGEX-4T-1 (GE Healthcare), respectively ...
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bioRxiv - Genomics 2021Quote: ... and AsNRDE-3 amino acids 45-246 (HQ611973) were fused in frame with GST using the Bam H1 site of pGEX-6P-1 (GE Healthcare), the proteins were expressed in E ...
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bioRxiv - Genomics 2021Quote: ... we used the Ascaris proteins cleaved from GST-fusions bound to Glutathione Sepharose 4B by treatment with PreScission protease (GE Healthcare). AsWAGO-2 peptides (YASRRMFELTKQSRDDYRA and RNGGQETSSSSSGEAGHLDI ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved TPR-CHAT protein was eluted from GST beads and further fractionated by a Resource Q (AKTA Pure, GE Healthcare) in buffer ...
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bioRxiv - Biochemistry 2022Quote: ... soluble His-GST-APLFAD (or His-SUMO-APLFAD) was loaded on a 5 mL HisTrap FF column (GE Healthcare Life Sciences), pre-equilibrated in lysis buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... The PfPHB1-and PfPHB2-GST fusion proteins were purified via affinity chromatography from bacterial extracts using glutathione-sepharose (GE Healthcare, USA) using the soluble fractions of the lysed bacterial cultures ...
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bioRxiv - Biochemistry 2023Quote: ... Bound proteins were eluted by boiling in SDS–PAGE sample loading buffer and analyzed by western blot using an anti-GST antibody (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... Cleaved GST-tag and bZip construct were separated by gel filtration chromatography using Hi-Load 16/600 Superdex 200 pg (GE Healthcare) on AKTA pure in a 20 mM MES pH 6.0 ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusions were purified by incubating the bacterial soluble fraction with pre-equilibrated glutathione Sepharose bead slurry (GE Healthcare, Munich, Germany) for 1 hour at 4°C and eluting with 10 mM reduced glutathione ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Mlph antibody was affinity-purified from immune sera over a column of GST-Mlph-147-428 coupled to cyanogen bromide-activated Sepharose 4B (GE Healthcare) by standard procedure.
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bioRxiv - Biochemistry 2023Quote: ... affinity columns of GST-fused Asct2(NT)-antigen and GST-fused Asct2(CT)-antigen were produced by conjugating the antigens with HiTrap NHS-activated HP (GE Healthcare). To purify anti-Asct2(NT ...
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bioRxiv - Cell Biology 2023Quote: GST and GST-DysSR22-23 were produced in BL21 bacteria using a standard protocol and were purified on Glutathione Sepharose 4B (GE Healthcare) [55] ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant GST-RAPSYN was purified from the supernatant by GST-affinity chromatography (SA010010, Smart-Lifescience) and size-exclusion chromatography (17-0060-01, GE Healthcare). Purified GST-RAPSYN protein was digested with thrombin (T8021 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were obtained 24 hours after treatment and GST was purified on a 1 mL GSTrap HP column (GE Healthcare) with an AKTA purifier 10 FPLC system ...
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
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bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
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bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
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bioRxiv - Immunology 2023Quote: ... covered in 4+4 Whatman papers (GE Healthcare, UK) soaked in transfer buffer (192 mM glycine ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing GFP and OFP fusion proteins were pulled down with GST-tagged anti-GFP and anti-mCherry nanobodies pre-bound to glutathione– Sepharose-4B beads (GE Healthcare #17075601), respectively ...
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bioRxiv - Biophysics 2019Quote: GST-fusion proteins (UBAN domains of OPTN, ABIN, NEMO and Ub) were cloned into pGEX-4T-1 (GE Life Sciences, Freiburg, Germany) and site-directed mutagenesis was performed by PCR to introduce desired amino acid substitutions.
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bioRxiv - Plant Biology 2020Quote: ... coli codon optimized bZip domain of protein Cre06.g310500.t1.2 (amino acid position 141-203) was cloned into the GST-containing vector pGEX-4T-2 (GE Healthcare Life Sciences) by the company GeneScript ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was further purified through negative chromatography by passing through a GST-affinity column (GSTrap FF Columns, GE Healthcare Life Sciences) to remove free GST ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...
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bioRxiv - Biochemistry 2019Quote: ... 0.0027M KCl pH 8) and they were bound to a GST affinity column (GSTrap™ Fast Flow, GE Healthcare: 17-5131-02). The column was washed (0.02M NaPO4 ...
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bioRxiv - Cell Biology 2020Quote: ... coli DH5α strain and purification of GST fusion proteins was achieved by incubation with Gluthatione Sepharose 4B matrix (GE Healthcare, Buckinghamshire, UK). Sumo proteins were kept bound to matrix ...
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bioRxiv - Cell Biology 2024Quote: ... The strips were then washed three times with 5 mL of TBST-BSA and incubated with anti-GST horseradish peroxidase–conjugated antibody (GE Healthcare, RRID:AB_771429) diluted in TBST-BSA ...
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bioRxiv - Cell Biology 2020Quote: ... The purifications of these proteins from transformed bacterial cell extract were performed according to manufacturer instructions (GST bulk kit, GE Healthcare Life Sciences). The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences) ...
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bioRxiv - Developmental Biology 2020Quote: ... of FZD7 containing the epitope for the F7-Ab (Glycine 168 to Serine 209) was cloned into the bacterial GST expression vector pGEX-4T3 (GE Healthcare Life Sciences). The epitope was shortened from either end through sequential cloning to produce 10 different length epitopes ranging from the original 42 aa to 8 aa ...
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bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
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bioRxiv - Microbiology 2021Quote: ... Cleavage of the GST tag to induce cleaved fusion proteins was performed using PreScission protease (10 U/mg of fusion protein, GE Healthcare, Uppsala, Sweden) or thrombin protease (10 U/mg of fusion protein ...