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Citations for GE Life Sciences :
101 - 150 of 4767 citations for 26S Proteasome Non ATPase Regulatory Subunit 10 PSMD10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... the protein was subjected to ion exchange chromatography and loaded onto a HiLoad 26/10 Q Sepharose high performance column (GE Healthcare), and eluted at ∼350 mM NaCl with a salt gradient from 0 M to 1.5 M NaCl in buffers containing 25 mM Tris-HCl (pH 7.7) ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0, 0.15 M NaCl) using a HiPrep 26/10 column (GE Healthcare Life Sciences). The cleavage reaction was initiated by adding 6×His-tagged SUMO protease to a final concentration of ∼8 μg/mL to the protein solution ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM βME was added to reduce cysteine residues followed by buffer exchange using a HiTrap 26/10 Desalting column (GE Healthcare) in 25 mM HEPES (pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... Small- (Superose 6 10/300 GL) or large-scale (Superose 6 pre-packed XK 26/70) SEC columns (GE Healthcare, Chicago, IL) were run with 500 µL or 15 mL of supernatant ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged in a Beckman 45Ti rotor at 100,000 x g for 30 min at 4 °C and the supernatant applied to a HiLoad 26/10 Q Sepharose column (GE Life Sciences) equilibrated in 90 % buffer A and 10% buffer B (buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... using a Superdex 200 Hiload 26/600 column (GE Healthcare) equilibrated with SEC buffer (10 mM DTT added to buffer where ColE9 contained free cysteine residue) ...
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bioRxiv - Biochemistry 2023Quote: ... and size exclusion chromatography (Superdex 200 26/600, GE Healthcare), farnesylated by enzymatic modification ...
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bioRxiv - Biophysics 2019Quote: ... and subsequently desalted and buffer exchanged (in to 20 mM phosphate buffer) using a HiPrep 26/10 Desalting column (GE Healthcare, Piscataway, NJ). The purity of affinity purified histidine tagged CsgF was investigated using SDS PAGE and the presence of the histidine tag was confirmed by western blot using an Anti-His (C-Term)-HRP antibody (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: Approximately 33 mg of CBL or 10 mg of mSLAP2 were loaded individually onto a HiLoad 26/60 Superdex 75 gel filtration column (GE Healthcare Life Sciences) equilibrated with GF buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The fractions containing the eluted protein were pooled and immediately subjected to buffer exchange using HiPrep™ 26/10 desalting column on an AKTA FPLC purification system (GE Healthcare, USA) into storage buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... pooled and concentrated to 10 ml and applied to a 320 ml size exclusion column (HiLoad ® 26/600 Superdex ® 200, GE Healthcare). The column was equilibrated with 30 mM PIPES pH 7.4 buffer containing 100 mM NaCl + 1 mM TCEP+ 15 μM PLP and eluted with the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... Free Alexa Fluor 555 was then separated from RepA-Titinx-Alexa Fluor 555 using a HiPrep 26/10 desalting column (GE Healthcare, Piscataway, NJ). The labeled protein sample was dialyzed into storage buffer (40 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... size exclusion chromatography (SEC) (Superdex 200 pg 26/600, GE Healthcare) was performed (50 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... size exclusion chromatography on a Superdex 200 26/60 (GE Healthcare) column was performed in SEC buffer (20 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... in which case a Superdex 75 26/60 column (GE Healthcare) operated at a 2.6 ml/min flow rate was used ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... All inhibitors were purified by SEC (S75, 26/60, GE Healthcare) in Buffer D ...
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bioRxiv - Immunology 2022Quote: ... 50 mM imidazole) before eluting the protein (HBS, 250 mM imidazole) following desalting using a HiPrep™ 26/10 Desalting column (GE Healthcare, Diegem, Belgium) to remove imidazole ...
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bioRxiv - Immunology 2022Quote: ... The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare, Diegem, Belgium). The Avi-His6-tag was cleaved by caspase-3 (produced in-house ...
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bioRxiv - Biochemistry 2021Quote: ... reaction mixture was terminated by heating and limit-digested products were recovered by doing centrifugation (10000 × g) for 10 min and then applied to Toyopearl S40 resin containing column (GE Healthcare-XK 26/100) for purification ...
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bioRxiv - Biochemistry 2019Quote: ... and further purified by size exclusion chromatography (S200 26/60; GE Healthcare) in buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and desalted on a Sephadex G25 XK 26/35 column (GE Healthcare). Next ...
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bioRxiv - Synthetic Biology 2020Quote: ... and a HiLoad 26/60 Superdex 75 gel filtration column (GE Healthcare). For Selenomethionine (SelMet)-labeling ...
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bioRxiv - Cancer Biology 2023Quote: ... loaded onto a HiLoad 26/600 Superdex 75 column (GE Healthcare Biosciences) and eluted with storage buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... specifically Superdex 200 Increase 26/600 (GE Healthcare, cat. GE28-9893-34), to ensure thorough cleaning and separation of the proteins ...
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bioRxiv - Systems Biology 2019Quote: ... using a 5-ml HiTrap Chelating HP column charged with Ni2+ ions followed by buffer-exchange chromatography on a HiPrep 26/10 desalting column (both GE Healthcare Life Sciences, Pittsburgh, PA) using an ÄKTAxpress system (GE Healthcare Life Sciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... and the σA subunit were expressed and purified by chromatography on HisTrap HP (GE Healthcare) Ni2+-affinity columns as described (Hu et al. ...
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bioRxiv - Biophysics 2020Quote: ... Selected fractions were subsequently loaded onto a Superdex 75 26/60 (GE Healthcare) at 20 °C and eluted in PBS ...
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bioRxiv - Biochemistry 2019Quote: ... and further purified by Superdex-75 (26/600) size-exclusion chromatography (GE Healthcare). The protein concentration was assessed using the A280 extinction coefficient of 71,740 M-1.
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT on a HiLoad Superdex 75 26/60 column (GE Healthcare). After treatment with Prescission protease overnight ...
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bioRxiv - Neuroscience 2020Quote: ... gel filtration column HiLoad 26/600 Superdex 200 pg (GE healthcare, IL, USA) and anion exchange column HiTrap Q HP (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... followed by Gel Filtration on a HiLoad Superdex 75 26/60 (GE Healthcare) column pre-equilibrated in 20 mM Tris (pH 8) ...
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bioRxiv - Biophysics 2022Quote: ... and passed over a Superdex 200 26/60 gel filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 8.0 at room temperature) ...
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bioRxiv - Biophysics 2022Quote: ... and passed over a Superdex 75 26/60 gel filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 8.0 at room temperature) ...
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bioRxiv - Biochemistry 2019Quote: ... loaded on a HiLoad 26/600 Superdex 200 column (GE Healthcare, Chicago, IL) pre-equilibrated with buffer E (HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... and run over a Superdex 200 26/600 gel-filtration column (GE Healthcare) in buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... and SEC on a Superdex prep grade 200 26/60 column (GE Healthcare) in 20 mM Tris/HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Excessive imidazole was removed using a Sephadex XK 26/60 column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... size-exclusion column (SEC) using HiLoad 26/600 Superdex 200 (GE Healthcare, USA) was performed to separate thrombin and the GST-tag cleaved protein in a SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... and further purified through a SD200HL 26/600 gel filtration column (GE Healthcare) in strep buffer supplemented with 1 mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... equipped with a 25 mL self-packed 20/26 GSTrap column (GE HealthCare). A linear gradient containing 50 mM reduced glutathione in “lysis buffer” was used for elution ...
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bioRxiv - Developmental Biology 2021Quote: ... OTP non-targeting pool (GE Healthcare) was used as control siRNAs in all experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05, GE Healthcare Dharmacon) or with siRNAs targeting PDK2 and PYCR1 (Dharmacon ...
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bioRxiv - Biochemistry 2022Quote: ... ON-TARGETplus SMART Pool Non-targeting (D-001810-10) and CHMP2A (L-020247-01) siRNAs were obtained from GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2020Quote: ... The eluate is then applied onto a sephacryl 500 (GE healthcare, HIPREP 26/60) equilibrated in 20 mM Hepes and 8 M urea ...
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bioRxiv - Biochemistry 2020Quote: ... and applied to a HiLoad 26/60 Superdex 200 size-exclusion column (GE Healthcare) at a flow rate of 0.5 mL min-1 ...
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bioRxiv - Neuroscience 2020Quote: ... the protein was loaded onto a Superdex 200 26/600 HiLoad column (GE Healthcare) with 5 mM MOPS ...
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bioRxiv - Biochemistry 2022Quote: ... samples were run over a Superdex 200 26/60 size-exclusion column (GE Healthcare) equilibrated with HEPES buffer ...
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bioRxiv - Biophysics 2019Quote: ... then loaded onto a 320 mL HiLoad 26/600 Superdex 200 column (GE Healthcare) equilibrated in gel filtration buffer [10 mM Tris-HCl ...
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bioRxiv - Biophysics 2019Quote: ... After further purification by size-exclusion chromatography (HiLoad 26/600 Superdex 75; GE Healthcare) in crystallization buffer (20 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... prior to loading to a HiLoad Superdex 200 pg 26/60 column (GE Healthcare) equilibrated with buffer C (20 mM HEPES ...