Labshake search
Citations for GE Life Sciences :
301 - 350 of 4767 citations for 26S Proteasome Non ATPase Regulatory Subunit 10 PSMD10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... Eluate fractions were pooled and loaded onto a size exclusion column (HiLoad Superdex 26/600 200 prep grade, GE Healthcare) pre-equilibrated with Tris buffer (50 mM Tris ...
-
bioRxiv - Microbiology 2023Quote: Size exclusion chromatography (SEC) was performed using an ÄKTAprime Plus with HiPrep 26/60 Sephacryl 400 HR column (GE Healthcare) at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... BepA protein was concentrated to ~60 mg/ml by ultra-filtration and then further purified on a HiLoad Superdex 200 26/600 column (GE Healthcare) equilibrated in buffer D ...
-
bioRxiv - Cell Biology 2020Quote: Images were analyzed automatically by the IN Cell Developer Toolbox software (cat. 25-8098-26, GE Healthcare Bio-Sciences, Marlborough, MA). Nuclei of living and dead cells were identified by using the corresponding fluorescent signal of Hoescht33342 ...
-
bioRxiv - Microbiology 2021Quote: ... The digested proteins were then passed over a 5mL HisTrap-FF column and the flow-through was collected for further purification by gel filtration chromatography using a HiLoad Superdex 75 26/600 pg column (GE Healthcare). The monodisperse peak corresponding to BBK32-C was pooled following analysis of chromatography fractions by SDS-PAGE and exchanged into HBS (10mM HEPES (pH 7.3) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Size exclusion chromatography was performed to further purify proteins using AKTA FPLC using an S200 26/600 filtration column (GE Healthcare), following Khandokar et al ...
-
bioRxiv - Molecular Biology 2020Quote: ... The fractions containing the protein were pooled and applied onto a size-exclusion chromatography column (HiLoad 26/600 Superdex 200 pg, GE Healthcare) pre-equilibrated with lysis buffer ...
-
bioRxiv - Microbiology 2022Quote: ... The eluted fractions were concentrated to 5 ml and loaded onto a HiLoad 26/600 Superdex 200 prep grade (GE Healthcare) equilibrated with the SEC buffer (50 mM Tris-HCl pH 8.5 ...
-
bioRxiv - Microbiology 2019Quote: ... BepA protein was concentrated to ~60 mg/ml by ultra-filtration and then further purified on a HiLoad Superdex 200 26/600 column (GE Healthcare) equilibrated in buffer D ...
-
bioRxiv - Microbiology 2020Quote: ... The final purification step for all variants encompassed a size exclusion chromatography run (HiLoad 16/60 or 26/60 Superdex 75 or 200 prepgrade, GE Healthcare) using a buffer with 25 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2019Quote: ... Fractions containing the eluted protein were concentrated with a 30 kDa molecular mass cutoff centrifuge concentrator (Amicon) and loaded onto a HiLoad 26/60 Superdex-200 size-exclusion column (GE Healthcare) pre-equilibrated with 150 mM NaCl ...
-
bioRxiv - Biochemistry 2021Quote: ... and subsequently purified further by size-exclusion chromatography (SEC) using either a HiLoad 16/600 or HiLoad 26/600 Superdex 75 pg column (GE Healthcare) equilibrated with a buffer containing 10 mM HEPES pH 8.0 and 150 mM NaCl ...
-
bioRxiv - Molecular Biology 2020Quote: ... The eluted proteins were then treated overnight with recombinant HRV 3C protease to remove the N-terminal His-tag and further purified via size exclusion chromatography on a HiLoad 26/600 Superdex 75 column (GE Healthcare) operating with 20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... LaGH65 was produced and purified as previously described (20) and further purified using size-exclusion chromatography on a HiLoad 26/60 Superdex 75 column (GE Healthcare). The LaGH13_31B Y295A variant was generated using primers in Table S3 and the QuickChange II Site-Directed Mutagenesis kit (Agilent ...
-
bioRxiv - Biophysics 2021Quote: ... Pooled protein fractions were deglycosylated with endoglycosidase F1 overnight at 4C and then concentrated for further purification with a size exclusion column (HiLoad 16/60 or 26/60 superdex 75 prep grade columns, GE Healthcare) in a buffer containing 20 mM Hepes (pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: ... The full length hTop2α was first purified by a tandem affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE healthcare) bound to Ni2+ ions followed by a StrepTrap HP column (GE healthcare) ...
-
bioRxiv - Biochemistry 2020Quote: ... The peak was concentrated and further purified by size-exclusion chromatography using a HiLoad Superdex 200 PG 26/600 column (GE Healthcare) equilibrated with GF buffer (10 mM HEPES ...
-
bioRxiv - Microbiology 2020Quote: ... EDTA was added to 1 mM and the PykA was then loaded onto a HiLoad 26/60 Superdex 200 Prep Grade gel filtration column (GE Healthcare) equilibrated in buffer C (500 mM NaCl ...
-
bioRxiv - Biophysics 2021Quote: ... The three encapsulins used in this study were purified via size exclusion chromatography (SEC) using a HiPrep 26/60 Sephacryl S-500 HR column (GE Healthcare) equilibrated with 50 mM HEPES pH 7.4 ...
-
bioRxiv - Cell Biology 2021Quote: ... Size exclusion chromatography was performed to separate FLAG-Ddi1 from the cleaved SUMO protein or the full-length His-SUMO-FLAG-Ddi1 using a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) in a buffer of 20 mM Tris HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2022Quote: ... The GST-tag was removed by a second affinity chromatography step with glutathione-agarose and the proteins in the flow-through were further purified by size exclusion chromatography using an Äkta Purifier System equipped with a HiLoad 26/600 Superdex 200 column (GE Healthcare). Protein-containing fractions were pooled and dialyzed against storage buffer (30 mM HEPES ...
-
bioRxiv - Immunology 2021Quote: ... Affinity-purified proteins were then purified by size exclusion chromatography using a HiLoad 26/600 Superdex 200 pg column (GE Healthcare).
-
bioRxiv - Immunology 2020Quote: ... Proteins were concentrated and subjected to size-exclusion chromatography on a HiLoad 26/600 Superdex 200 pg (GE Healthcare; Cat# 28989336). Trimer-containing fractions were pooled ...
-
bioRxiv - Molecular Biology 2022Quote: ... size-exclusion chromatography of all proteins was carried out on either HiLoad 26/60 or 16/600 Superdex 75 prep grade column (GE Healthcare) in 20 mM Tris pH 8.0 ...
-
bioRxiv - Molecular Biology 2022Quote: ... CtNaa5082-289 was subsequently purified by SEC (size-exclusion chromatography) using a Superdex 75 26/60 gel filtration column (GE Healthcare) in buffer G500 (20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2019Quote: ... The GyrA protein was purified by nickel-affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE Healthcare) bound to Ni2+ ions ...
-
bioRxiv - Microbiology 2019Quote: ... the His6-tag was removed using TEV protease and the protein was subjected to size exclusion chromatography (HiPrep 26/60 Sephacryl S-200; GE Healthcare) using a buffer consisting of 40 mM HEPES pH 7.4 and 150 mM NaCl ...
-
bioRxiv - Cell Biology 2020Quote: ... The resulting SNX9 and His6x-β2-hinge+appendage proteins were separately applied to a HiLoad 26/600 Superdex 200 pg column (GE Healthcare). Peak fractions of target proteins were collected and concentrated using Amicon Ultra-15 10K Centrifugal filters (Sigma-Aldrich).
-
bioRxiv - Plant Biology 2020Quote: ... and protease by IMAC and purified further by size-exclusion chromatography using either a HiLoad 16/600 Superdex 75 PG column or HiLoad 26/600 Superdex 75 PG column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5 and 150 mM NaCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 mM EDTA (pH 6.8) prior to purification by size exclusion chromatography using a gel filtration column (HiLoad 26/60 Superdex 75 column, GE Healthcare). The sample was concentrated using a 10 kDa molecular weight cut-off centrifugal filter (Merck Millipore) ...
-
bioRxiv - Microbiology 2020Quote: ... to a volume of 2 ml and applied to size exclusion chromatography (SEC; HiLoad 26/600 Superdex 200 pg, GE Healthcare), After SEC ...
-
bioRxiv - Biochemistry 2020Quote: ... the 6x His tag was cut off by the TEV protease and the protein was further purified by size-exclusion chromatography on a Superdex 75 HiLoad 26/60 column (GE Healthcare) in SEC buffer (20 mM Na2HPO4 ...
-
bioRxiv - Biochemistry 2021Quote: ... the TTPN protein was concentrated and loaded on a size-exclusion chromatography (SEC) column (HiLoad 26/60 Superdex 75 pg, GE Healthcare). Peak fractions from the first SEC step ...
-
bioRxiv - Biochemistry 2021Quote: ... eMAT was further purified by size exclusion chromatography (SEC) using a HiLoad® 26/600 Superdex 200 pg column (GE Healthcare) in SEC Buffer A (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... Untagged Elp proteins were then further purified by size exclusion chromatography using a HiLoad 26/600 Superdex 75 PG gel filtration column (GE Healthcare) in HBS (10 mM HEPES (pH 7.3) ...
-
bioRxiv - Biophysics 2022Quote: ... Fractions containing Aβ40/Aβ42 were lyophilised and further purified using a Superdex 75 HR 26/60 column (GE Healthcare, Chicago, IL), and eluates were analyzed using SDS−polyacrylamide gel electrophoresis for the presence of the desired protein product ...
-
bioRxiv - Cell Biology 2022Quote: ... His8-tag containing recombinant proteins were purified using Ni2+-NTA agarose affinity column followed by size exclusion chromatography (Superdex 200 26/60 column from GE healthcare) in a final buffer containing 100 mM NaCl ...
-
bioRxiv - Neuroscience 2023Quote: ... Final polishing of the target proteins was done through size exclusion on a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare) eluting in PBS with 1 mM DTT ...
-
bioRxiv - Microbiology 2022Quote: ... The sample was incubated at 4°C for 30 minutes and further purified using a HiLoad 26/600 Superdex 200 (GE healthcare).
-
bioRxiv - Microbiology 2022Quote: ... The supernatant was loaded onto a nickel column (TBS 0.2% LDAO 25mM imidazole) and the eluted sample (250 mM imidazole) further purified by size exclusion chromatography (HiLoad 26/600 Superdex 200 GE healthcare) using 10 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... CpxA31-163 was passed over a HiLoad Superdex 75 26/60 size-exclusion column equilibrated with resuspension buffer on an ÄKTA purifier (GE Healthcare). The CpxA31-163 elution peak fractions were pooled and concentrated using a 3,000 MWCO spin concentrator.
-
bioRxiv - Biochemistry 2023Quote: ... The protein was further purified by size exclusion chromatography (SEC) with a HiLoad 26/60 Superdex 75 pg column (GE Healthcare) in SEC buffer (20 mM Sodium-phosphate buffer pH 6.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Eluates containing myrArf1•GDP were further purified by size exclusion chromatography (SEC) using a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare) pre-equilibrated and then run in an isocratic manner using fresh TNMD buffer at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Concentrated FhES (10 mg total protein) was fractionated (2 mL/fraction) by SEC on a Sephacryl S-200 HR 26/60 column (GE Healthcare) connected to an AKTA Start automated liquid chromatography system (GE Healthcare) ...
-
bioRxiv - Biochemistry 2023Quote: ... monomeric actin was separated from oligomeric species by passing it through a HiPrep 26/60 Sephacryl S-200 HR size-exclusion column (GE Healthcare) equilibrated in G-buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... The refolded WT- and E111V-GCAP1 variants were purified by size exclusion chromatography (SEC, HiPrep 26/60 Sephacryl S-200 HR, GE Healthcare) followed by anion exchange chromatography (AEC ...
-
bioRxiv - Biophysics 2024Quote: ... the remaining 10 ml of concentrated 6-His-AdhE in buffer A were loaded onto a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) equilibrated with buffer A ...
-
bioRxiv - Biophysics 2024Quote: ... We then further purified the supernatant by size exclusion chromatography on a HiLoad 26/600 Superdex 75 pg column (GE Healthcare) pre-equilibrated with the same buffer ...
-
bioRxiv - Microbiology 2024Quote: ... protein was further purified by size exclusion chromatography on a HiPrepTM 26/60 SephacrylTM S200 HR gel filtration column (GE Healthcare). Protein was eluted isocratically at a flow of 1 ml/min ...
-
bioRxiv - Cell Biology 2021Quote: ... a non-targeting scramble siRNA (#D-001210-01-05, GE Healthcare Dharmacon) was used in the experiments shown in figure 3 and supplementary figure 6C while a pool of four scrambled siRNAs (#D-001206-13-05 ...