Labshake search
Citations for GE Life Sciences :
51 - 100 of 4767 citations for 26S Proteasome Non ATPase Regulatory Subunit 10 PSMD10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... except containing 80 mM NaCl and no imidazole) using a HiPrep 26/10 desalting column (GE Healthcare). Next ...
-
bioRxiv - Microbiology 2022Quote: ... The fractions containing CapRelSJ46 were pooled and applied on a HiPrep 10/26 desalting column (GE Healthcare) pre-equilibrated with storage buffer (buffer D ...
-
bioRxiv - Plant Biology 2022Quote: ... HyTh-FDX: Fractions were pooled and loaded onto a HiPrep 26/10 desalting column (GE Life Sciences) into 50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2023Quote: ... Ammonium sulfate was eliminated by a desalting column step (HiPrep 26/10 desalting, GE Healthcare; ÄKTA Avant). The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg ...
-
bioRxiv - Cell Biology 2022Quote: ... Non-targeting siRNA ON-TARGETplus (D-001810-10-05) (Dharmacon, GE Healthcare) served as the siRNA control (“siRNA scramble”) ...
-
CHD7 interacts with the nucleosome acidic patch for its efficient activity via its N-terminal regionbioRxiv - Biochemistry 2020Quote: ... and Superdex 200 26 600 (GE healthcare) in a buffer containing 150 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... column (XK 26/20, GE Healthcare/Cytiva) equilibrated with 20 mM KPi pH 7 at 5 ml/min ...
-
bioRxiv - Immunology 2023Quote: ... Monomeric antibodies were purified by size exclusion chromatography on a Superdex 200 26/600 column (GE Healthcare) in 25 mM Citrate ...
-
bioRxiv - Biochemistry 2023Quote: ... Ammonium sulfate was removed by injection on a desalting column (HiPrep 26/10 desalting, GE Healthcare; ÄKTA Avant). The sample was concentrated before separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg ...
-
bioRxiv - Cancer Biology 2023Quote: ... SEC-eluted samples were subject to buffer exchange on Fast-Desalt FPLC column HiPrep 26/10 (GE Healthcare) in 50mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
-
bioRxiv - Microbiology 2022Quote: SEC analysis of single proteins or combinations of the subunits was conducted using AKTA basic 10 FPLC system (GE healthcare) and a Superdex 200 Increase 10/300 SEC column ...
-
bioRxiv - Biochemistry 2021Quote: Fractions containing the dIG8-CC protein were then buffer-exchanged to buffer B (20mM Tris·HCl, 150 mM sodium chloride, pH 7.5) in a HiPrep 26/10 desalting column (GE Healthcare), and incubated overnight at 4°C with inhouse-produced His6-tagged TEV peptidase at a peptidase:substrate ratio of 1:20 (w/w ...
-
bioRxiv - Biochemistry 2020Quote: ... Fractions containing TrxA-UbiTPa were pooled and detergent was removed using a Hiprep 26/10 Desalting column (GE Healthcare) preequilibrated with buffer D ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... the active fractions were combined and desalted with a desalting column (HiPrep 26/10 Desalting, GE Healthcare, München, Germany), using MilliQ water as elution buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... and the ones containing both His-SKA1/2+GFP-His-SKA2 subunits were pooled and further purified using size exclusion chromatography over a Superose 6 Increase 10/300 GL (GE Healthcare) column equilibrated with gel filtration buffer (20 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: ... The assembled capsids are subsequently purified from un-assembled dimer subunits using a Superose 6 10/300 GL size exclusion column (GE Healthcare). The small molecule allosteric modulators were purchased (Chem-Div ...
-
bioRxiv - Molecular Biology 2020Quote: ... HiLoad 26/60 Superdex 200 pg (GE Healthcare), was equilibrated with 240 ml of PBST (Sigma ...
-
bioRxiv - Plant Biology 2019Quote: ... or HiLoad 26/600 pg column (GE Healthcare), equilibrated in 20 mM sodium citrate pH 5.0 ...
-
bioRxiv - Biochemistry 2021Quote: ... and Superdex S200 26/60 column (GE Healthcare). The purified protein was concentrated and stored at -80 °C.
-
bioRxiv - Microbiology 2019Quote: ... Samples were further purified by gel filtration chromatography using either a Superdex 75 (26/60) or Superdex 200 (26/60) column attached to an AKTA-format FPLC (GE Healthcare) and PBS as a running buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by a final polishing step of the proteins by size-exclusion chromatography on an Äkta Purifier System using either HiLoad 26/600 Superdex 200 or HiLoad 26/75 Superdex columns (GE Healthcare). Purified proteins were dialyzed against immunization buffer (150 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... The proteins were concentrated and further purified by size exclusion chromatography (Hiload Superdex 26/60 200 pg or Hiload Superdex 26/60 75 pg, GE Healthcare). The fractions containing pure protein were pooled ...
-
bioRxiv - Microbiology 2024Quote: ... and then subjected to size-exclusion chromatography using either an S75/26–60 column or an S200/26–60 (GE Healthcare) in the same buffer as before but without imidazole ...
-
bioRxiv - Cell Biology 2020Quote: ... Peak TopBP1b6-8 fractions (eluted with 50mM and 75mM imidazole) were desalted (HiTrap™ or HiPrep 26/10, GE Healthcare) in physiological buffer (10mM Na2H/NaH2 PO4 pH 7.6 ...
-
bioRxiv - Biochemistry 2020Quote: The sample was desalted into Buffer A using a HiPrep 53 ml 26/10 desalting column (GE Healthcare, Chicago, IL) with 14 ml injections at 9 ml/min for all steps ...
-
bioRxiv - Biochemistry 2022Quote: ... Samples collected from IMAC were exchanged into size exclusion chromatography (SEC) buffer (20 mM Na2HPO4, 150 mM NaCl, pH 7.4) using either a HiPrep 26/10 desalting column (GE Healthcare) or through multiple rounds of concentration and dilution using an Amicon Ultra-15 filter unit with a 10 kDa molecular weight cut-off ...
-
bioRxiv - Biochemistry 2019Quote: ... Fractions containing tagged-SbtB7001 were pooled and transferred into binding buffer using a HiPrep 26/10 Desalting column (GE Healthcare). The His6–Ub tags were removed using a ubiquitin carboxyl-terminal hydrolase 2 catalytic core domain (USP2cc):SbtB7001 molar ratio of 1:25 at 4 °C overnight as previously described [14] ...
-
bioRxiv - Microbiology 2019Quote: ... Eluates were buffer exchanged into gel filtration buffer (25 mM Tris-Cl, 150 mM NaCl, pH 7.5) via HiPrep 26/10 desalting column (GE Healthcare), followed by overnight incubation with Ulp-1 to cleave off the His6-tagged SUMO moiety ...
-
bioRxiv - Immunology 2022Quote: ... The purified protein buffer was exchanged to PBS containing 0.5 mM TCEP using a HiPrep 26/10 desalting column (GE Healthcare).
-
bioRxiv - Biochemistry 2023Quote: ... proteins were eluted with 500 mM imidazole followed by buffer exchange to buffer (50mM Tris, 300 mM NaCl, pH 8.0) with HiTrap 26/10 Desalting column (GE Healthcare). To obtain protein without sumo tag ...
-
bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
-
bioRxiv - Biochemistry 2020Quote: ... or Superdex 200 PG 26/600 column (GE Healthcare). It was pre-equilibrated in Buffer B ...
-
bioRxiv - Plant Biology 2020Quote: ... or a HiLoad 26/600 pg column (GE Healthcare), equilibrated in 20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... using a HiLoad Superdex 200 26/60 (GE Healthcare) equilibrated with 20 mM HEPES buffer (pH 7.2 ...
-
bioRxiv - Biochemistry 2023Quote: ... HiLoad 26/60 Superdex 75 pg column (GE Healthcare) was utilised in the size exclusion chromatography of the desired fragments in 20 mM Sodium-phosphate buffer pH 6.5 ...
-
bioRxiv - Biochemistry 2024Quote: ... and loaded onto a Superdex 26/600 (GE Healthcare) pre-equilibrated with 20 mM Tris pH 7.5 at 20 °C ...
-
bioRxiv - Biochemistry 2019Quote: ... The labeling reaction was quenched after 1 hr with fresh DTT at 100 mM final concentration and loaded onto a HiPrep 26/10 desalting column (GE Healthcare). The final labeled proteins were concentrated to at least 80 µM and flash-frozen in Protein Buffer (50 mM Tris-HCl [pH 7.2] ...
-
bioRxiv - Biochemistry 2021Quote: ... Pooled fractions containing target protein was subjected to buffer exchange with buffer A using a HiPrep 26/10 desalting column (GE Healthcare). Next ...
-
bioRxiv - Bioengineering 2021Quote: ... The biotinylation mixture was incubated at room temperature for 1 h and then loaded onto a HiPrep 26/10 desalting column (GE Healthcare) pre-equilibrated with a storage buffer (1×PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 mM βME was added to reduce cysteine residues followed by buffer exchange using a HiTrap 26/10 Desalting column (GE Healthcare) in 25 mM HEPES (pH 7.5 ...
-
bioRxiv - Biophysics 2019Quote: ... The DTT in the buffer for cysteine mutant of K48-diUb was removed by desalting (HiPrep 26/10 Desalting column, GE Healthcare), and the protein was reacted with the pre-mixed excess Alexa488 and Cy5 dyes ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fractions containing target protein were pooled and exchanged back to 100% buffer A using a Sephadex desalting column (HiPrep 26/10 column, GE Healthcare). TEV cleavage was performed overnight at 23°C with 1 mM DTT ...
-
bioRxiv - Biophysics 2020Quote: Proteins were further purified by size-exclusion chromatography (Superdex 200 HiLoad 26/60 or Superdex S200 Increase 10/300 GL; GE Healthcare) on an AKTA pure fast protein liquid chromatography system (GE Healthcare) ...
-
bioRxiv - Biophysics 2022Quote: ... proteins were concentrated by ultrafiltration at 4 °C (MWCO: 10 kDa) and passed over a Superdex 75 26/60 gel filtration column (GE Healthcare) equilibrated in 20 mM sodium phosphate pH 7.4 ...
-
bioRxiv - Biochemistry 2022Quote: ... Eluted fractions were then exchanged into native binding buffer using a HiPrep Desalting 26/10 column on an ÄKTA pure FPLC (GE Healthcare). N-terminal tags were removed from Elp proteins using His-tagged tobacco etch virus (His-TEV ...
-
bioRxiv - Biochemistry 2022Quote: ... The fractions with an absorbance at 512 nm were combined and loaded as described above onto a pre-equilibrated desalting column (HiPrep 26/10 Desalting, GE Healthcare) with a desalt buffer (200 mM sodium phosphate buffer at pH 7.5) ...
-
bioRxiv - Cell Biology 2023Quote: ... All proteins were further purified by gel filtration on preparative Superdex HiLoad 26/600 or analytical (Superdex 200 10/300) columns (GE Healthcare).
-
bioRxiv - Biochemistry 2023Quote: ... The flow through was then concentrated in an Amicon Ultra-15 50,000 MWCO spin concentrator to ∼10-20 mg/mL and loaded onto a HiPrep 26/60 S200 Size Exclusion Column (GE Healthcare), equilibrated in Buffer D ...
-
bioRxiv - Biochemistry 2023Quote: ... the protein solution was concentrated to ∼6 mL using a centricon (MW cutoff = 10 kDa) and was purified via size exclusion chromatography with a HiLoad 26/600 Superdex 75 pg column (GE Healthcare). The purified protein solution was diluted to 135 μM (∼2.0 mg/mL ...