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Citations for GE Life Sciences :
4801 - 4850 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare). Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... detection of antibodies was detected using the Amersham ECL Western Blotting Prime Blocking and Blotting Reagents (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... followed by anti-rabbit IgG horseradish peroxidase-linked secondary antibodies (GE Healthcare Life Sciences, UK Ltd., 1:10,000) and Pierce ECL Plus Western Blotting Substrate (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were then probed with horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, GE Healthcare, NA934, RRID: AB_772206) for 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... were used in combination with secondary anti-mouse antibodies conjugated to horseradish peroxidase (GE Healthcare NXA931, 1:5000). For visualization ...
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bioRxiv - Immunology 2023Quote: ... Antibody purification from cell supernatants was done in two steps using the ÄKTA Avant purification system (GE Healthcare). The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe ...
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bioRxiv - Molecular Biology 2024Quote: ... The following secondary antibodies were used: anti-mouse IgG (NA0310V) and anti-rabbit IgG (NA9340V) from GE Healthcare for immunoblotting ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-rabbit HRP antibody (50 μL/well of a 1:1000 dilution, GE Healthcare, 1h at RT), with washing steps as above ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed in 1 x TBST and incubated in the appropriate secondary antibodies (anti-Rabbit, GE Healthcare, NA934V ...
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bioRxiv - Neuroscience 2022Quote: ... the oligomers were separated from the monomeric and fibrillar forms of the protein by size exclusion chromatography on a Superose 6 HR10/300 column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA) equilibrated in PBS pH 7.4 at a flow rate of 0.5 mL/min ...
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bioRxiv - Bioengineering 2020Quote: Experimental “affinity” between β-CD monomers and protein-conjugates were measured experimentally through surface plasmon resonance (SPR) with a Biacore X100 system (GE Healthcare Bio-Sciences, Pittsburgh, PA) according to previous protocols 31,32 ...
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bioRxiv - Biochemistry 2021Quote: Size exclusion chromatography (SEC) of the concentrated protein solution was conducted using a HiLoad™ 26/600 Superdex™ 200 pg column (GE Healthcare, Mu-nich, Germany) coupled to the aforementioned chromatography system ...
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bioRxiv - Cancer Biology 2019Quote: ... The immune complexes were mixed with 20 μl of Protein A or G sepharose 4 Fast flow beads (50% v/v, GE Healthcare Life Sciences, Marlborough, MA, USA). Whole cell lysates and immunoprecipitates were detected by Western blotting ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the purest form of the proteins were loaded on a size exclusion chromatography HiLoad® 16/60 Superdex® 200 pg column (GE Healthcare Life Sciences / Cytiva), pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA ...
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bioRxiv - Neuroscience 2023Quote: ... Separation of aSYN monomers and PFFs was performed with fast protein liquid chromatography using a Superose 6 10/300GL column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA). PFFs were briefly sonicated ...
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bioRxiv - Microbiology 2020Quote: ... Anti-rabbit IgG HRP-linked secondary antibody and 0.45-μm polyvinylidene difluoride (PVDF) membrane were obtained from GE healthcare. Specific-Pathogen-Free (SPF ...
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bioRxiv - Genetics 2021Quote: ... The following antibodies were used: anti-GFP (7.1/13.1, mouse monoclonal IgG, secondary antibody peroxidase conjugated sheep anti-mouse IgG, NA931V, GE healthcare) or anti-Flag (M5 ...
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bioRxiv - Developmental Biology 2021Quote: ... For monoclonal antibody production, the DNA encoding mouse KCTD19 (residue 654-793 aa, NP_808459.1) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Immunology 2021Quote: ... or Amersham ECL anti-mouse IgG horseradish peroxidase-linked species-specific whole antibody (NA931, GE Healthcare UK Limited, UK) in the blocking buffer for 3 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse immunoglobulin (Ig)-G horseradish peroxidase (HRP)-linked secondary antibody (Amersham catalogue number NA931, GE Healthcare, Buckinghamshire UK) diluted 1:4000 was incubated at RT for 2 hours before final washes and 3,3’,5,5’-Tetramethylbenzidine (TMB ...
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bioRxiv - Genetics 2020Quote: ... Three washes were performed with TBST followed by 1 h incubation with secondary antibody (ECL, HRP linked, GE Healthcare) and another series of washes ...
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bioRxiv - Microbiology 2019Quote: ... membranes were incubated for 1 hour with secondary α-rabbit antibodies conjugated with horseradish peroxidase (HRP) (GE healthcare, NA934) diluted by 1:10,000 in 5% skim milk in TBST ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were washed with TBS-T and incubated with HRP secondary antibodies (GE Healthcare Life Sciences, 1:2000), and immunobands were detected with a Pierce ECL Western Blotting Substrate (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... before they were incubated with HRP-conjugated secondary antibodies (1:10000, NXA931 and NA934, GE Healthcare Europe, Freiburg, DE) also diluted in blocking solution for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactivity was detected using species-appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000; GE Healthcare, Cleveland, OH, USA). Western blots were imaged via HRP chemiluminescent detection (Azure Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... and visualized using HRP-conjugated secondary antibodies (donkey anti-rabbit, 1:15,000, and sheep anti-mouse, 1:15,000, GE Healthcare) and ECL substrate (SuperSignal West Femto Maximum Sensitivity Substrate and Atto Ultimate Sensitivity Substrate ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibody conjugated with horseradish peroxidase was detected with ECL plus chemiluminescence kit (Amersham Biosciences/GE Healthcare, Piscataway NJ). The images were acquired with an HP Scanjet and densitometry determined using NIH ImageJ ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... An enhanced chemiluminescence (ECL)-horse radish peroxidase (HRP) conjugated secondary antibody (NA934V, 1:1000 dilution, GE Healthcare, Uppsala, Sweden) and SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL, GE Healthcare, Chicago, IL). Polyclonal affinity purified NS3 and NS5 proteins were produced as previously described (20) ...
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bioRxiv - Microbiology 2020Quote: ... the filters were incubated with HRP-conjugated secondary antibodies in the respective blocking agent (NA931V and NA934V, GE Healthcare) for 1-2 h at RT or overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were detected against mouse or rabbit IgGs and visualized with ECL Western blot detection solution (GE Healthcare) or Odyssey infrared imaging system (LI-COR ...
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bioRxiv - Plant Biology 2022Quote: ... Anti-rabbit (for PIF3, RGL2, ABI5 and UGPase) or antimouse (for PHYB) IgG HRP-linked whole antibody (GE healthcare) in a 1:10,000 dilution was used as secondary antibody for 2 hours at RT ...
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bioRxiv - Microbiology 2020Quote: ... Anti-rabbit IgG HRP-linked secondary antibody and 0.45-μm polyvinylidene difluoride (PVDF) membrane were obtained from GE healthcare. Specific-Pathogen-Free (SPF ...
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bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with horseradish peroxidase-conjugated sheep anti-mouse IgG polyclonal antibody (GE Healthcare, Chicago, IL, USA). Antigen-antibody interactions were detected using o-phenylenediamine solution as the substrate (Nacalai Tesque) ...
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bioRxiv - Biochemistry 2021Quote: ... HRP-conjugated secondary antibodies were detected with WesternBright ECL chemiluminescent HRP substrate using a ImageQuant imaging unit (GE Healthcare). Band intensities for reference protein standards and neuropil extracts were calculated in ImageJ ...
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bioRxiv - Cell Biology 2020Quote: ... Signals from HRP-conjugated secondary antibodies were visualized by chemiluminescence (Amersham ECL start Western Blotting Detection Reagent, GE Healthcare) and recorded using the ImageQuant LAS 4000 Imaging System (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... PVDF membranes were washed and revealed using horseradish peroxidase (HRP)-conjugated secondary antibodies and ECL detection reagent (GE Healthcare). The blots were imaged on a Geliance gel imaging systems (PerkinElmer ...
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bioRxiv - Microbiology 2022Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
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bioRxiv - Biophysics 2022Quote: ... Secondary antibody staining using either sheep α-mouse or donkey α-rabbit (NA931V, NA934V, GE HealthCare, Chicago, IL, USA) was performed for 1 hour on a tube roller at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... For all primary mouse antibodies an anti-mouse-HRP in a dilution of 1:2000 was used (GE Healthcare). All blots were blocked with TBS-T 5% Milk and washed 3x for 15 minutes with TBS-T after each antibody incubation ...
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bioRxiv - Microbiology 2022Quote: ... The antibodies were purified on an AKTA FPLC using GE Superdex 200 increase 10/300 GL column (GE HealthCare) in 1× PBS.
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bioRxiv - Microbiology 2022Quote: ... antibodies diluted 1:500 in 1 U/mL DNAse I in Tris Buffered Saline containing 1% BSA (GE Healthcare). Three 5-minute washes with block (1% BSA with 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2022Quote: ... HRP-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies were purchased from GE Healthcare (Chicago, IL, USA). Doxycycline ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was incubated with secondary antibodies and visualized with the ECL Detection System (GE Healthcare, Little Chalfont, UK).
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... a CM5 sensor chip was covalently modified by an anti-GST polyclonal goat antibody (GST capture Kit, GE Healthcare). The surface of flow cell 1 and 2 was activated for 60 s with 50 mM NaOH (10 μL/min) ...
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bioRxiv - Cell Biology 2024Quote: ... Visualization of the antibody binding sites was achieved using Amersham ECL Prime Western Blotting (GE Healthcare UK Ltd, RPN2232) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2024Quote: ... at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences, Marlborough MA) at a 1:10,000 dilution ...