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Citations for GE Life Sciences :
4551 - 4600 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: The affinity of the sE protein for the pr peptide was measured by SPR using a Biacore T200 system (GE Healthcare Life Sciences) equilibrated at 25ºC ...
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bioRxiv - Biochemistry 2023Quote: ... The 5 mL sample concentrated from the fractions involving the target proteins with Amicon Ultra-15 10 kDa was loaded on Resource Q (GE Healthcare Life Science) anion-exchange column equilibrated with buffer A using the FPLC systems ...
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Rhodococcus equi Escapes Macrophage Autophagy: VapA Inhibits ATP6V0d1-Mediated Lysosomal DegradationbioRxiv - Immunology 2023Quote: ... second antibody (1:10000) for 1 h at room temperature and then proteins were visualized with enhance chemiluminescence (ECL) kit purchased from GE Healthcare (Chicago, USA). And detection reagent and analyzed using GE Amersham Imager 600.
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bioRxiv - Neuroscience 2023Quote: ... Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham, GE Healthcare Life Sciences). The membrane was blocked in 5% skimmed milk (HIMEDIA ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 μg of the whole protein extracts was separated by SDS gel electrophoresis and transferred to a nitrocellulose membrane (Whatman, GE Healthcare, Freiburg, Germany). Rabbit polyclonal anti-SDHC (1:200 ...
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bioRxiv - Immunology 2023Quote: ... The culture medium containing the secreted LALA-B3B9 recombinant immunoglobulin G (rIgG) was purified using the protein A affinity column (GE Healthcare, Chicago, IL). The eluted fractions from purification were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the purity of the purified antibody ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and anti-mouse-ICAM-1 mAb (clone YN1/1.7.4) were produced by culturing hybridoma cells and purified using protein G sepharose chromatography (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Biophysics 2023Quote: ... His6-tagged fusion proteins were isolated from the resulting supernatant by affinity chromatography using Ni2+ Sepharose High-Performance beads (GE Healthcare, Pittsburgh, PA). Bound fusion proteins were washed with 200-300 mL of column Buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... Antibodies were purified from filtered culture supernatants by fast protein liquid chromatography (FPLC) on an ÄKTA Pure instrument using a 1 mL HiTrap MabSelect PrismA column (Cytiva, formerly GE Healthcare Life Sciences). Purified mAbs were concentrated and buffer changed into PBS using Amicon® Ultra-4 50KDa Centrifugal Filter Units (Millipore Sigma) ...
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bioRxiv - Biophysics 2023Quote: Both mFE- and tFE-labeled proteins were further purified by size exclusion chromatography (SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) in a buffer containing 20 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... The products were filtered by 10 kDa-cutoff Amicon Ultra centrifugal filters to remove DNA and proteins and then analyzed by a Mono Q 5/50 GL column (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Biophysics 2023Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labeled protein on a Superose 6 gel-filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Free biotin (or dye) was removed using size-exclusion chromatography by loading the labelled protein on a Superose 6 gel filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... and immuno-reactive protein bands were detected by the ECL system and visualized by imaging analyzer (ImageQuant LAS 500, GE Healthcare Life Sciences).
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bioRxiv - Biophysics 2024Quote: ... Germany) and gel filtration fast protein liquid chromatography (FPLC) was performed on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). The sample was dialyzed against phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Cellular lysates were cleared by centrifugation (14,000 rpm, 20 min) and then incubated (30 min) with Protein G-Sepharose beads (GE Healthcare, Mississauga, ON, Canada) followed by subsequent centrifugation (14,000 rpm ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The dye-labeled protein was purified from unreacted free dye using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50,000 rpm for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... University of Pennsylvania School of Medicine) [61] and HuR (19F12, Thermo) 400 μL of 10% (v/v) protein A Sepharose CL-4B beads (GE Healthcare Life Sciences) in IP-2 buffer [50 Mm Tris pH 7.9 ...
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Evolutionary Adaptation of an HP1-protein Chromodomain Integrates Chromatin and DNA Sequence SignalsbioRxiv - Genetics 2024Quote: ... The protein was further purified using tandem ion exchange chromatography with HiTrap Q HP and HiTrap SP HP columns (Cytiva/GE Healthcare Life Sciences). Digested protein was first diluted three-fold with low salt buffer (20 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 h at 4°C on a rocker followed by an incubation with 200 μl of a 20 % slurry of protein A sepharose beads (GE Healthcare, Uppsala, Sweden) for 1 h at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated on SDS-PAGE and fluorescently labelled proteins were detected by in-gel scanning with the Typhoon FLA 9000 (GE Healthcare Life Sciences) using Cy3 settings (532nm excitation and 610PB filter).
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bioRxiv - Biochemistry 2024Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labelled protein on a Superose 6 gel filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies for chemiluminescence detection were donkey anti-rabbit-HRP (GE healthcare, NA 9340V, 1:5000) or sheep anti-mouse-HRP (GE healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... membranes were probed with HRP conjugated anti-IgG secondary antibodies and ECL (GE Healthcare Life Sciences). The antibodies used were ...
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bioRxiv - Immunology 2021Quote: Purification of the recombinant antibodies was conducted on a ÄKTAprime plus system (GE Healthcare Life Sciences) at 4°C using a 1 mL HiTrap® Protein A HP pre-packed Protein A Sepharose® column (Cytiva) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the bound antibodies were visualized by chemiluminescence using the ECL plus reagent (GE Healthcare, Buckinghamshire, UK). The GAPDH content was analyzed as the loading control using a rabbit polyclonal antibody.
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies were detected using mouse IgG HRP linked whole Ab (1:4000; NXA931; GE healthcare).
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bioRxiv - Biochemistry 2020Quote: ... samples were incubated with appropriate antibodies and nProtein A Sepharose™ 4 Fast Flow (GE healthcare) or Ni-NTA Agarose (QIAGEN ...
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated with Horseradish Peroxidase (HRP)-conjugated secondary antibody (GE healthcare, NXA931V or NA934V) in 1% non-fat BSA in PBST at room temperature for 1 hour ...
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bioRxiv - Systems Biology 2020Quote: ... then incubated with anti-rabbit and anti-mouse HRP conjugated secondary antibody (GE Healthcare Life Sciences) at 1:2000 for 2hrs at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Secondary horseradish peroxidase (HRP)-conjugated antibody dilutions were used as follows: goat α-rat (GE Healthcare) 1:2,000 ...
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bioRxiv - Systems Biology 2020Quote: ... The following secondary antibodies were used for immunoblotting: ECL rabbit HRP-linked IgG (GE Healthcare, NA934) and ECL mouse HRP-linked IgG (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Incubation using the secondary antibody α-mouse IgG (H&L)-HRP (1:10,000) (GE healthcare #NXA931) diluted in blocking buffer was performed for two hours ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase-conjugate Anti-Mouse (NA931) and Anti-Rabbit (NA934) antibodies are obtained from GE healthcare. For Western blot standard enhanced chemiluminescence reagents (WBLUF0100 ...
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bioRxiv - Neuroscience 2022Quote: ... Bound antibodies were detected by applying biotinylated goat anti-rabbit IgG (GE Healthcare, Velizy Villacoublay, France) for 2h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated with HRP conjugated secondary antibody (IgG; mouse or rabbit) (GE Healthcare UK) at 1:5000 dilution in blocking buffer and incubated at RT with shaking for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were probed with an ECL anti-rabbit IgG secondary antibody (1:10,000) from GE Healthcare Life Science and imaged using a ChemiDoc imaging system (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were washed and immunoreactive bands were developed for five minutes with ECF (GE Healthcare) and revealed in a Chemidoc Imaging System (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... either a 1:10,000 dilution of α-mouse-HRP conjugated antibody (GE Healthcare Life Sciences NA931) or a 1:2,000 dilution of α-mouse-HRP conjugated antibody (used for western blot of immunoprecipitations ...
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bioRxiv - Neuroscience 2022Quote: ... and reincubated with the secondary HRP-conjugated antibody (1:2000 dilution for anti-rabbit, GE Healthcare, NA934 ...
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bioRxiv - Cell Biology 2022Quote: ... antibody-antigen complexes were detected by FUSION SOLO S (VILBER) using an ECL system (GE Healthcare). Quantification was performed via densitometry using Fusion Capt software (VILBER) ...
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bioRxiv - Cell Biology 2022Quote: ... and Donkey anti-rabbit IgG(H+L) antibody conjugated with HRP (GE Healthcare, NA934V, 1:10,000). Antibody signals were detected using SuperSignal West Pico Plus Chemiluminescent Substrate (Thermofisher ...
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bioRxiv - Molecular Biology 2020Quote: ... and then incubated for 1 h with ECL mouse IgG HRP-conjugated whole antibody (GE Healthcare) or rabbit IgG HRP-conjugated whole antibody (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... Horseradish peroxidase-coupled secondary antibodies (anti-mouse and anti-rabbit, GE Healthcare; anti-rat, Jackson Immunoresearch) in combination with Clarity Western ECL Substrate (Biorad ...
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bioRxiv - Neuroscience 2022Quote: ... The binding of HRP-conjugated secondary antibodies was revealed by ECL Plus (GE Healthcare Life Sciences) and chemiluminescence was measured using a ChemiDoc XRS apparatus (BioRad) ...
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bioRxiv - Molecular Biology 2019Quote: ... sheep anti-mouse horseradish peroxidase-linked antibody (1:3000, NA931V, GE Healthcare Life Sciences, Uppsala, Sweden), donkey anti-rabbit horseradish peroxidase-linked antibody (1:4000 ...
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bioRxiv - Molecular Biology 2019Quote: ... donkey anti-rabbit horseradish peroxidase-linked antibody (1:4000, NA934V, GE Healthcare Life Sciences, Uppsala, Sweden).
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bioRxiv - Genetics 2019Quote: ... and the secondary antibodies were visualized by ECL Prime Western Blotting Detection Reagents (GE Healthcare, UK).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBST before incubation with secondary HRP-conjugated antibodies (GE Healthcare). Membranes were further washed three times with TBST before ECL exposure.