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Citations for GE Life Sciences :
4701 - 4750 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... After incubation with secondary HRP-conjugated antibody (R&D) blots were developed using ECL reagent (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... samples were incubated with anti-LptD antibodies and nProtein A Sepharose™ 4 Fast Flow (GE healthcare) at 4°C over-night with slow rotation ...
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bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... Membranes were washed in TBS-Tween and incubated with secondary HRP-conjugated antibody (GE Healthcare, Chicago, Illinois) at RT for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... The labelled antibodies were purified by passing through Disposable PD 10 Desalting Columns (GE Healthcare Life Sciences), concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... The blots were further incubated with anti-rabbit or anti-mouse HRP conjugated secondary antibodies (GE Healthcare) and visualized using ECL (GE Healthcare) ...
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bioRxiv - Plant Biology 2019Quote: ... Immunodetection was carried out using specific antibodies and an ECL Plus Western Blotting Detection System (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... These were detected with appropriate secondary antibodies conjugated to HRP and visualized by ECL-Plus (GE Healthcare).
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then incubated for 75 min with the secondary antibody coupled to horseradish peroxidase (GE Healthcare). After four washing steps with TBST (5 min each) ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblots were washed with TBS-T and incubated with HRP-conjugated secondary antibodies (GE Healthcare, 1:5,000) for one hour in room temperature before development in films ...
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bioRxiv - Microbiology 2020Quote: ... and revealed with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:3,000; GE Healthcare, USA). Blots were developed using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... washed and incubated in secondary antibody before enhanced chemiluminescence detection using an ImageQuant LAS4000 imager (GE Healthcare). The primary antibodies used for WBs prepared using murine liver lysates were rabbit anti-LDLR (1:5,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with anti-Rabbit HRP-conjugated secondary antibody (1:5000 of NA934, GE Healthcare) for 1 hour at room temperature and subsequently washed with PBST thrice ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated for two hours with horseradish peroxidase (HRP) conjugated secondary antibodies (GE Healthcare, Helsinki, Finland) at 1:6000.
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bioRxiv - Plant Biology 2021Quote: ... and secondary horseradish peroxidase-conjugated antibodies 1:10 000 in PBS 0.1% Tween-20 (Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Plant Biology 2021Quote: ... and secondary horseradish peroxidase-conjugated antibodies 1:10 000 in PBS 0.1% Tween-20 (Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Cell Biology 2020Quote: ... and secondary antibodies HRP-coupled anti-mouse or anti-rabbit (GE Healthcare and Jackson Laboratories, 1:5000). The cell cycle analysis was performed using HMLE cells that were fixed in 70% ethanol for 1 h on ice ...
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bioRxiv - Physiology 2021Quote: ... or anti-goat IgG antibodies and Amersham ECLTM Prime Western Blotting Detection Reagent (GE Healthcare, Buckinghamshire, UK). Band quantification was carried out using an Image Station 2000R (Kodak ...
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bioRxiv - Biochemistry 2020Quote: The antibodies binding properties were analyzed at 25 °C on a Biacore ™ 8K instrument (GE Healthcare) using 10mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... while the secondary antibody (1/10,000) was a commercial Amersham ECL-HRP linked rabbit IgG (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The membrane was then incubated with an HRP-conjugated secondary antibody (ECL anti-rabbit IgG, GE Healthcare) and PstS bands were visualized with the ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: Secondary antibodies for western blot analysis were ECL Plex goat anti-rabbit IgG-Cy5 (PA45012, GE Healthcare), ECL Plex goat anti-mouse IgG-Cy3 (PA43009 ...
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bioRxiv - Immunology 2021Quote: Purified antibodies targeting S1 were quantified with SPR assay using the BIAcore 8000 system (GE Healthcare, USA) carried out at 25°C in single-cycle mode ...
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bioRxiv - Immunology 2020Quote: ... and immunodetected using appropriate primary and peroxidase-coupled secondary antibodies (GE Healthcare Life Sciences, Pittsburg, PA, USA). Proteins were visualized by West Pico and West Dura chemiluminescence Substrate (Thermo Fisher ...
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bioRxiv - Genetics 2020Quote: ... These primary antibodies were detected with HRP-conjugated anti-mouse or anti-rabbit (1:5000; GE HealthCare), using the SuperSignal™ West Pico chemiluminescent substrate (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... Secondary antibody treatment utilized goat -anti-rabbit HRP conjugate or goat-anti-mouse-HRP conjugate (GE Healthcare) at 1:10,000 and 1:5000 dilutions ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes incubated with an anti-mouse HRP-linked secondary antibody (1:5000, GE Healthcare, NXA931, RRID:AB_772209). Either Pierce ECL Western Blotting Substrate (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified on Fy-4 immobilized on a HiTrap NHS- activated HP column (GE Healthcare). Antibodies were eluted using Pierce Gentle Ag/Ab Elution Buffer (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Purified GST-Slp1(456-488aa) was detected with either goat anti-GST HRP-conjugated antibodies (GE Healthcare) or rabbit polyclonal anti-pThr480 antibodies.
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bioRxiv - Immunology 2024Quote: ... The bispecific antibody was further polished by cation exchange chromatography using Capto MMC (GE Healthcare, Princeton, NJ) and ion exchange chromatography using Q HP (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... primary antibodies were used together with horseradish peroxidase-conjugated α-mouse immunoglobulin G (1:5000, GE Healthcare) as secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... antibodies were then incubated for 1 hour and revealed with the enhanced ECL detection system (GE Healthcare). The primary antibody against P2X7 (#APR-004 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were detected using either HRP-linked donkey anti–rabbit IgG (GE Healthcare, #NA934V; 1:10,000) or HRP-linked sheep anti– mouse IgG (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: HRP-conjugated secondary antibodies raised against the appropriate species were used: anti- mouse IgG (GE Healthcare, #NA931), anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Monomeric antibodies were purified by size exclusion chromatography on a Superdex 200 26/600 column (GE Healthcare) in 25 mM Citrate ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated with anti-rabbit or anti-mouse secondary antibodies (GE Healthcare Life Sciences, Pittsburgh, PA) for 1h at room temperature and rinsed in 3 washes of TBST for 10 min each ...
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bioRxiv - Biochemistry 2022Quote: ... followed by incubation with anti-rabbit or anti-mouse secondary antibodies (GE Healthcare Life Sciences, Pittsbugh, PA). Detection was performed with SupersignalTM Chemiluminescent substrate (Thermo Fisher ...
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bioRxiv - Genetics 2024Quote: ... Incubation with secondary antibodies for 1.5 hours was followed by chemiluminescence development system (GE Healthcare, Pittsburgh, USA). Antibodies details are listed in Supplemental Table 2-3.
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bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
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bioRxiv - Immunology 2019Quote: ... Cells were harvested and lysed in PBS supplemented with 1 mM PMSF and soluble protein was purified using nickel-affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare, Piscataway, New Jersey, USA). After SDS-PAGE analysis ...
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bioRxiv - Systems Biology 2021Quote: ... Samples were centrifuged at 16,000 x g for 20 minutes and the supernatant used for affinity purification of biotinylated proteins using 25 µl of washed streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301) for 16 hours with rotation at 4℃ ...
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bioRxiv - Systems Biology 2021Quote: ... The lysate was boiled in Laemmli SDS-PAGE sample buffer for five minutes and 50 µg of protein per lane were separated onto 10% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science, Cat# 10600001). Following Ponceau S staining ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were centrifuged at 15,000 × g for 15 minutes and the supernatant was used for biotinylated protein capture using 15 μl of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... A tenth of the lysate was taken as TCE control and the rest was added to washed Protein G-coupled Sepharose beads (Sigma-Aldrich GE Healthcare 17-0618-01) for preclearing (30 min) ...
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Bioengineering 2021Quote: ... A second purification step was performed for IDO-Gal3 using size exclusion chromatography in an ÄKTA Pure Protein Purification System (GE Life Sciences, Marlborough, MA, USA). Molecular weight and purity were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Neuroscience 2021Quote: ... Control human blood samples were processed to remove the most abundant proteins using albumin and IgG depletion SpinTrap columns (GE Healthcare Cat 28-9480-20) following the manufacturers instructions ...
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bioRxiv - Pathology 2021Quote: ... 250 μg of total protein was loaded on a 13 cm Immobiline dry-strip (pH 4-7, M/s. GE Healthcare Life Sciences, Amersham, UK), on a rehydration tray ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were first purified with affinity chromatography using AKTA pure 25 system and HiTrap MabSelect SuRe protein A column (GE healthcare, Cat# 11-0034-94) for huACE2-IgHu-pVax ...