Labshake search
Citations for GE Life Sciences :
401 - 450 of 572 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The dye-labeled protein was purified using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50000 rpm for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... for 30 minutes with or without cofactor GMPPNP (30 μM) then applying 0.5 mL to a PD-10 Sephadex G-25 gel-filtration column (GE Healthcare). Eluted fractions (0.5 mL ...
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bioRxiv - Biochemistry 2021Quote: ... The excess Cu(II) was eliminated by passing the protein through a PD MidiTrap G-25 desalting column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 and 150 mM NaCl.
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bioRxiv - Biophysics 2022Quote: ... The sample was exchanged into the D2O buffer using a NAP-5 Sephadex G-25 column (GE Healthcare, Chicago, IL). For dUMP and TMP (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... CM were collected after 48h of transfection and filtered through 0.45 μm syringe filters and used for protein purification via ÄKTA pure 25 FPLC (GE Healthcare) equipped with 1-ml HisTrap affinity column (HisTrap HP ...
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bioRxiv - Microbiology 2019Quote: ... Eluates were buffer exchanged into gel filtration buffer (25 mM Tris-Cl, 150 mM NaCl, pH 7.5) via HiPrep 26/10 desalting column (GE Healthcare), followed by overnight incubation with Ulp-1 to cleave off the His6-tagged SUMO moiety ...
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bioRxiv - Immunology 2019Quote: ... RPMI 1640 with L-glutamine and 25 mM HEPES (cat. # SH30255.01) and ECL reagent (cat. # RPN2106) were from GE Healthcare Life Sciences (Logan ...
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bioRxiv - Biochemistry 2019Quote: ... The most concentrated IR-ECD-containing fraction was either desalted using disposable 8.3 ml Sephadex G-25 PD-10 desalting columns (GE Healthcare) equilibrated with HBS by centrifugation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The unreacted DBCO molecules were eliminated by a Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences). To make fluorescently labeled DBCO modified FBS proteins or individual proteins (BSA or APO H) ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation for 1 hour at room temperature proteins were purified using PD MiniTrap™ G-25 columns (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... The Illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit (Cat. No. 25-6601-96, GE Healthcare Life Sciences, UK) was used for viral genome amplification (expected avg ...
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bioRxiv - Biochemistry 2021Quote: ... with purification of labelled protein away from free label using a desalting Superdex G-25 (PD-10) column (GE Healthcare). FRET was examined using 495 excitation for FITC-HU-A ...
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bioRxiv - Microbiology 2021Quote: ... DTT was removed via two consecutive passes through a PD-10 Sephadex G-25 M buffer exchange column (GE Healthcare) as per the manufacturer’s instructions into labelling buffer (50 mM HEPES pH 7.1 ...
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bioRxiv - Immunology 2020Quote: ... The fractions containing the antibody were pooled and elution buffer was exchanged for PBS using NAP-25 columns (GE Healthcare). Purified VHH72hFc1X7 in PBS was quantified by absorbance at 280 nm using a Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... The excess DBCO-NHS was also removed by using Sephadex G-25 desalting columns (GE Healthcare Japan Co., Tokyo, Japan). The resulting DBCO-labeled lectin was then incubated at 4 °C with ten-times the molar amount of 5’-azide-modified DNA oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2022Quote: Titrations were performed at 25°C with a stirring speed of 750 rpm using the MicroCalTM iTC200 System (GE Healthcare) as previously described [41] ...
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bioRxiv - Biochemistry 2022Quote: ... 25 μl of pre-equilibrated protein L beads (Capto™ L resin, GE Healthcare Cat no. 17547802 Cat. no. 17547803) was added and reaction was incubated for 90 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Further purification was performed in column buffer (25 mM Tris HCl pH 8.0 and 150 mM NaCl) using HiLoad Superdex 200 gel-filtration chromatography (GE Healthcare). The peak fractions were collected ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Germany) and exchanged to the desired cell culture medium using Sephadex G-25 PD10 desalting columns (GE Healthcare Bio-Science). The final concentration and integrity of eGFP-WNT-3A in the conditioned medium were determined using both ELISA (GFP ELISA® kit ...
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bioRxiv - Neuroscience 2022Quote: ... The unbound fluorophores were removed by gel filtration on a NAP-5 column (Sephadex-25 medium, GE Healthcare, Munich, Germany). The final antibody concentration and degree of labelling was analyzed on a spectrophotometer (NanoDrop 2000 UV-Vis ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were dialyzed against 25 mM Tris (pH 8.0) and loaded onto a Q column (HiTrap Q Fast Flow, GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The BRCA1RING/BARD1RING and BRCA1RING/BARD1193 constructs were concentrated and further purified using a 120 mL Superdex 75 column on an AKTA FPLC system in SEC buffer (25 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 1 mM DTT; GE Healthcare). For the BRCA1RING/BARD1216 ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from plated cells or snap-frozen kidneys using the RNAspin Mini kit (GE Healthcare, #25-0500-72) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... gel filtration with a flow rate of 0.8 mL/min using FPLC systems (AKTA pure 25, GE Healthcare Life Science). The 5 mL sample concentrated from the fractions involving the target proteins with Amicon Ultra-15 10 kDa was loaded on Resource Q (GE Healthcare Life Science ...
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer and 0.150 M NaCl) using PD-10 desalting columns or Midi-Trap G-25 (GE Healthcare). The purity of the proteins was evaluated using SDS–PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Immunology 2024Quote: ... Liver infiltrating cells were obtained after 25 min centrifugation (600 g without brake) in a 35% and 67.5% bilayer Percoll (GE Healthcare) gradient ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were washed extensively with TBS-T before chemiluminescent detection using the ECL western Blotting Detection Reagents (GE Healthcare Life Sciences, RPN2106) and X-ray film or VersaDoc system (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... cleared lysates from ARL15A86L expressing Flp-In™ T-REx™ HeLa cells were incubated with streptavidin beads (17-5113-01, 5 ml, GE Healthcare). Trypsin digestion on beads was performed and peptides were collected in water ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were subsequently washed with 1 x TBS/T for 30 min in 10 min intervals prior to incubation in ECLTM (GE Healthcare, cat# RPN2106) and exposure by film (FUJIFILM Super RX ...
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bioRxiv - Molecular Biology 2019Quote: ... characterization of an N-terminal Aβ peptide DAE-EG (DAEFRHDSGYSGKQKSRNEGKGGC) binding to anti-mouse captured antibodies from hybridoma supernatants was performed using a BIAcore T-200 instrument (GE Healthcare, Marlborough, MA) [36] ...
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bioRxiv - Immunology 2020Quote: ... Fractions that yielded proliferation of TN T cells were pooled and subjected to cation exchange chromatography on a Mono S 5/200 GL column (GE Healthcare, Piscataway NJ). The active fractions were again pooled and subjected to buffer exchange (Amicon ultra 3k cutoff ...
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bioRxiv - Microbiology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated and depleted of CD8+ T cells in a single step by density centrifugation using FicollPaque PLUS (GE Healthcare Life Sciences), SepMate tubes (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... membranes were washed 3x in PBS-T and then incubated with appropriate secondary antibody conjugated with horseradish peroxidase (GE Healthcare, Chicago, IL, USA). Chemiluminescence was accomplished using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3 times 10 minutes each with TBS-T and then incubated with the corresponding IgG secondary antibodies horseradish peroxidase-conjugated (GE Healthcare Life Sciences) at 1:1000 for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... and at the indicated time points (T=4d, 8d, 12d, 16d, 20d) using a 4X objective (InCell 6000 Analyzer, GE Healthcare Life Sciences). Segmentation and counting of GFP- and mCherry-positive cells were performed using an Acapella script (PerkinElmer).
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bioRxiv - Plant Biology 2021Quote: ... 0.5 ml aliquots of xylem sap were filtered through Sephadex columns (PD SpinTrap® G-25, GE Healthcare, Chicago, Il, USA) using Tris-buffer (50 mM Tris HCl (Roth) ...
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bioRxiv - Cancer Biology 2021Quote: ... filtered using a 0.45 µm Puradisc 25 mm polyethersulfone Syringe Filter (6780-2504, GE Healthcare Life Sciences, Whatman™, Chicago, IL) to remove cellular debris ...
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bioRxiv - Cell Biology 2020Quote: Images were analyzed automatically by the IN Cell Developer Toolbox software (cat. 25-8098-26, GE Healthcare Bio-Sciences, Marlborough, MA). Nuclei of living and dead cells were identified by using the corresponding fluorescent signal of Hoescht33342 ...
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were seeded the day before the transfection to reach 60-70% confluence and were then transfected with 25 nM of siRNA using the DharmaFECT 1 reagent (Dharmacon, GE Healthcare) following the Dharmacon siRNA transfection protocol ...
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bioRxiv - Genomics 2020Quote: ... annealed with complementary strands to make double-stranded oligos and purified using G-25 spin columns (GE Healthcare, #27-5325-01). Binding reactions were set up as follows ...
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bioRxiv - Neuroscience 2019Quote: ... Labeled proteins were separated from the free fluorophore using a NAP-5 Sephadex G-25 desalting columns in labeling buffer (GE Healthcare). The labeling efficiency was determined using an extinction coefficient of 5400 M-1cm-1 at 335 nm for IAEDANS and the protein concentration was determined by Bradford assay using BSA as a standard ...
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bioRxiv - Biochemistry 2019Quote: ... dynein constructs were mixed with 20 μM Halo Alexa488 dye and incubated on ice for 10 min and a PD MiniTrap G-25 column (GE Healthcare) equilibrated with dynein gel filtration buffer was used to remove excess dye afterwards.
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was heat-inactivated for 5 min at 95°C and the free nucleotides were removed on a Sephadex G-25 column (GE Healthcare). The oligo was annealed to a 1.5x excess of the unlabelled complementary strand by heating for 5 min to 80°C and slowly cooling to RT in annealing buffer (50 mM Hepes ...
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bioRxiv - Genomics 2019Quote: ZHBTc4 YPet-MD TIR1-V5 mCherry-OCT4-AID cells were plated in a 96-well plate coated for 1 hour at 37°C with 1:25 diluted StemAdhere (Primorigen Biosciences #S2071-500UG) and imaged on an IN Cell Analyzer 2200 (GE Healthcare) using the TexasRed and Brightfield channels ...
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bioRxiv - Biophysics 2020Quote: ... centrifuged at 14000g and 4 °C for 5 min and the excess of ammonium sulfate was removed by running the supernatant through a NAP-25 column (GE Healthcare). The tAv was purified using HiTrap chelating column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 20-fold excess of CV3-25 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested ...
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bioRxiv - Systems Biology 2020Quote: ... the target-specific antibody was added to the cellular lysates together with 25 μl of Protein A or G sepharose (GE Healthcare) depending on the species of target antibody and the mixture was incubated overnight rotating at 4°C ...