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Citations for GE Life Sciences :
451 - 500 of 572 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The eluted protein was buffered exchanged into endotoxin-free phosphate buffered saline (PBS) using a sterile PD MidiTrap G-25 column (GE Healthcare). Finally ...
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bioRxiv - Microbiology 2020Quote: Surface plasmon resonance studies were performed at an analysis temperature of 25 °C using a Biacore T200 instrument and HBS-EP+ buffer (both from GE Healthcare). Mouse anti-human Fc monoclonal antibody R10Z8E9 was immobilised onto active and reference flow cells of a Series S sensor CM5 chip using an amine capture kit (both from GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer containing 8M urea ...
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bioRxiv - Microbiology 2020Quote: dHepaRG or PHH cells seeded into a 24-well plate were transfected with 25 nM or 10 nM of siRNAusing Dharmafect#1 (GE HealthCare) or Lipofectamine RNAiMax (Life Technologies) ...
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bioRxiv - Biophysics 2020Quote: ... Plexins and GTPases were further purified by either size exclusion chromatography or using a PD-10 Sephadex G-25 desalting column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... After that the labeled protein was purified from the excess of free dye by a PD SpinTrap G-25 column (GE Healthcare), divided into aliquots and stored at -80 °C.
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bioRxiv - Biophysics 2022Quote: ... and the excess of dye was removed by using a PD Minitrap G-25 column for NPM or a Superdex 200 10/300 GL (GE Healthcare) size exclusion chromatography column for SET/TAF-Iβ ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions containing the enzyme were pooled and applied to a Sephadex™ G-25 (medium) (GE Healthcare, Piscataway, NJ, USA) column equilibrated and eluted in 50 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... in presence of 32P end-labeled telomeric primers that has been purified using a micro-spin G-25 column (GE healthcare). PCR reactions were resolved by 9% polyacrylamide gel electrophoresis at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed thrice in PBS and plates were scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 25 μm; GE Healthcare). All staining steps were performed at room temperature for one hour ...
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bioRxiv - Microbiology 2021Quote: ... Protein-DNA complexes were incubated at 25 °C for 15 mins before injection onto a Superdex 200 Increase gel filtration column (GE Healthcare). The peak corresponding to DNA-protein complex was collected and concentrated ...
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bioRxiv - Biophysics 2020Quote: Size-Exclusion Chromatography-Multi Angle Light Scattering (SEC-MALS) experiment was performed at 25°C using a Superdex 200 10/300 GL column (GE HealthCare) connected to a miniDAWN-TREOS light scattering detector and an Optilab T-rEX differential refractive index detector (Wyatt Technology ...
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bioRxiv - Microbiology 2020Quote: ... amino acids 319-537) (Acro Biosystems, Newark, NJ) protein was measured at 25°C using BIAcore T200 surface plasmon resonance (SPR) (GE Healthcare). STI-1499 or STI-2020 antibody was covalently immobilized on a CM5 sensor chip to approximately 500 and 100 resonance units (RU) ...
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bioRxiv - Physiology 2020Quote: ... The labeled protein was subsequently purified using size exclusion chromatography on a Sephadex™ G-25 Medium column (GE Healthcare, UK). To remove excess free dyes from the labeled proteins and also avoid hydrolysis of extra fluorophore ...
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bioRxiv - Biochemistry 2019Quote: ... For the in vitro ATPase activity assay and pull-down assay the buffer of the proteins was exchanged via a PD SpinTrap G-25 spin columns (GE Healthcare) to activity assay buffer (25mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2019Quote: ... and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns, GE Healthcare).
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bioRxiv - Microbiology 2021Quote: Binding of C1 and its sub-components to GST-ErpB and GST-ErpQ was performed at 25°C using a Biacore T200 (GE Healthcare) as previously described (Garcia 2016) ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were separated on 12.5% (v/v) acrylamide gels of 25 cm x 21 cm x 1 mm using an Ettan DALTsix electrophoresis unit (GE Healthcare). The SDS-PAGE was first run at a current of 2 W/gel for 1 h and then at a constant current of 15 W/gel for 5 h ...
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bioRxiv - Synthetic Biology 2020Quote: ... The reaction was quenched by the addition of 20 μl water followed by desalting in a MicroSpin G-25 spin column (GE Healthcare). The ∼53 μl eluate was transferred to a PCR tube and placed under a 385 nm UV lamp (∼3 cm distance from the tops of the tubes ...
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bioRxiv - Pathology 2021Quote: ... After this time excess TCEP was removed by diafiltration into fresh BBS buffer using PD Minitrap G-25 columns (GE Healthcare) and the concentration was corrected to 20 μM ...
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bioRxiv - Molecular Biology 2019Quote: ... equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer containing 8M urea ...
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bioRxiv - Cell Biology 2019Quote: ... the antibodies were separated from unconjugated dye by gravity filtration through Sephadex G-25 DNA grade size exclusion columns (GE Healthcare) by visual detection ...
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bioRxiv - Biochemistry 2019Quote: For conjugation of pG to a SMCC-functionalized ODN an aliquot of pG was buffer exchanged to (100 mM Sodium Phosphate, 25 µM TCEP, pH 7) using a PD10 desalting column (GE Healthcare). Subsequently ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was inactivated at 75 °C for 10 min and the labeled RNA/DNA was purified by gel filtration (Illustra MicroSpin G-25 columns; GE Healthcare). Recombinant ...
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bioRxiv - Biochemistry 2021Quote: ... The click-reaction was performed by incubation for 30 min at 20 °C in the dark with vigorous intermittent shaking and the free biotin was removed from the clicked extracts using PD MiniTrap G-25 column (GE Healthcare) equilibrated with IPD buffer ...
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bioRxiv - Biochemistry 2021Quote: ... A 30 μl aliquot of a given reaction was quenched by the addition of 20 μl water followed by desalting in a MicroSpin G-25 spin column (GE Healthcare). The NPOM (caged ...
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bioRxiv - Cancer Biology 2020Quote: ... the reaction was quenched by addition of the same EDTA solution and the labeled construct was purified using gel-filtration chromatography (Sephadex G-25, PD10 desalting column; GE Healthcare) into 0.9% saline ...
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bioRxiv - Biophysics 2021Quote: ... U-13C-enriched proteins were purified through Ni-NTA affinity column and further by gel filtration chromatography on an ÄKTA Pure 25 FPLC (GE Healthcare) using Superdex 75 Increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: Scale-up and process development for trimer production in fed-batch mode was performed in a ReadyToProcess WAVE 25 bioreactor system using ActiCHO P medium (GE Healthcare) and the respective feed media ActiCHO Feed a and ActiCHO Feed B (both GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... The sample was concentrated using Vivaspin-20 10 kDa and further purified via SEC using ÄKTA Pure 25 (GE Life Sciences) equipped with Superdex 75pg 16-600 column (GE Life Sciences) ...
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bioRxiv - Microbiology 2023Quote: SEC analysis was performed using a Superdex 75 10/300 GL prepacked column connected to ÄKTA pure 25 chromatography system (GE Healthcare). The column was equilibrated with a 50 mM potassium phosphate buffer (pH 7.0) ...
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bioRxiv - Cell Biology 2023Quote: ... Rab35BD and Rac1BD-Rab35BD) (40 µL; 10-25 mg/ml) were loaded onto a Superdex-200 increase 5/150 column (GE Healthcare) or BioSec5 300 (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... and pellets were resuspended in 6 mL 25 % Standard Isotonic Percoll (Percoll: GE Healthcare, 17-5445-02 with 10% 10x PBS) in Medium A ...
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bioRxiv - Biochemistry 2023Quote: ... The elutions were pooled and concentrated to 500 µl before passing over a PD MiniTrap G-25 desalting column (GE Healthcare) equilibrated with 50 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2023Quote: Coupled PEG-PhyB and PEG-PIF6 were purified by size exclusion chromatography using a HiLoad Superdex 200 pg (16/600 or 25/600, GE Healthcare) column connected to an ÄKTA Explorer system with PBS as a running buffer ...
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bioRxiv - Biochemistry 2023Quote: ... was then centrifuged at 21,000 ×g at 4 °C for 10 min and 100 µL of the supernatant was loaded on to a Superdex 200 column (25 mL, GE Healthcare) equilibrated with the Buffer A ...
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bioRxiv - Immunology 2023Quote: ... Liver infiltrating leukocytes were isolated by centrifugation at 600g for 25 minutes using a 35% and 70% bilayer Percoll (GE Healthcare) gradient ...
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bioRxiv - Biochemistry 2024Quote: The quaternary structures were determined by size exclusion chromatography using a 25 mL Superdex 200 10/300 GL Increase column (GE Healthcare) for FDP_E ...
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bioRxiv - Biophysics 2024Quote: All SPR binding experiments were performed on a CM5 chip (Cytiva) at 25 °C using a Biacore T200 Instrument (GE Healthcare). The purified CNBH domains were immobilized on the CM5 chip using a standard amine coupling chemistry in the presence of 10 mM sodium acetate buffer at pH 5.5 as the immobilization buffer (buffer used to directly dissolve ligands) ...
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bioRxiv - Immunology 2024Quote: ... The expressed SOSIPs were purified as described previously (13, 25) using PGT145 immunoaffinity chromatography (Sepharose 4B resin, GE Healthcare Life Sciences) and eluted with 3 M MgCl2 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mm × 1 mm × 1 mm) and brain FDG-PET were acquired simultaneously on a hybrid 3 T SIGNA PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the CUB Hôpital Erasme (Brussels ...
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bioRxiv - Immunology 2021Quote: ... The blots were washed in TBS-T and incubated in secondary antibody in antibody buffer for 2 hours before washing again in TBS-T before visualising with ECL (GE Healthcare, Chicago, IL, USA) and imaging in an ALLIANCE 6.7 Chemiluminescence Imaging System (UVITEC ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were imaged for GFP and mCherry 24 h after initial plating (t=0) and at the indicated timepoints using a 4X objective InCell Analyzer system (GE Healthcare Life Sciences, Marlborough). Segmentation and counting of GFP- and mCherry-positive cells were performed using an Acapella script (PerkinElmer ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with 0.1% PBS-T and were incubated with secondary horseradish peroxidase-coupled antibodies for 1h at RT (GE Healthcare, Jackson ImmunoResearch, 1:10000). ECL Western Blotting Detection Reagent (RPN2106 ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were washed (3x) with TBS-T and probed with the species-matching secondary horseradish peroxidase-conjugated antibody: donkey anti-rabbit (GE Healthcare, dilution 1:10.000) or sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Physiology 2023Quote: ... with TBS-T and incubated (1-hour, room temperature) with HRP-linked anti-rabbit secondary antibody (1:10,000; GE Healthcare Bioscience, Baie d’Urfe, Canada). Chemiluminescence was assessed using the Luminata™ Crescendo Western HRP Substrate (Millipore ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 5 times with PBS-T and incubated with a 1:5000 dilution of anti-mouse IgG-HRP (GE healthcare cat. # NA931-1ml), or with a 1:100000 dilution of anti-ferret IgG-HRP (Abcam cat ...
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bioRxiv - Biochemistry 2020Quote: ... for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences). Method for EMSA was modified from a previously published protocol (30) ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted fraction was neutralized with 2 M Tris pH7.0 and either diluted first 1:1 with 20 mM phosphate pH7.0 buffer (25 mL milk sample) or loaded directly (100 mL milk sample) onto a Q Sepharose FF (GE Healthcare, Uppsala, Sweden) equilibrated with 20 mM phosphate pH7.2 buffer column at 150 cm/h and 90 cm/h ...
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bioRxiv - Molecular Biology 2021Quote: ... Double stranded Oligonucleotides were end-labeled with T4 polynucleotide kinase in the presence of [γ-32P]ATP and purified using G-25 columns (GE Healthcare). To remove secondary structures present in them ...