Labshake search
Citations for GE Life Sciences :
251 - 300 of 572 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... MRI images were acquired with the aid of a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI) and an 8-channel head coil ...
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bioRxiv - Microbiology 2023Quote: ... The relevant oligonucleotide of SV40 large T antigen NLS (PPKKKRKVED) was ligated into the pGEX2T vector (GE Healthcare) carrying the GFP gene at the C-terminus of the multi-cloning site to produce GST-SV40NLS-GFP protein (referred to as SV40 NLS) ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were then washed 3 times in TBS-T and bands visualized with Amersham ECL chemiluminescence reagent (GE Healthcare). Blots were stripped (62.5 mM Tris-HCl pH 6.5 ...
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bioRxiv - Microbiology 2019Quote: Direct binding of His6-SidI to SusF was assessed by SPR using a Biacore T-200 instrument (GE Healthcare) at 25 °C ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were washed with TBS-T and incubated with HRP secondary antibodies (GE Healthcare Life Sciences, 1:2000), and immunobands were detected with a Pierce ECL Western Blotting Substrate (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed using 0.1% TBS-T and proteins were detected using ECL or ECL Select (GE Healthcare, USA) on ChemiDoc XRS+ System (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary CD8+ and CD4+ T cells were isolated from buffy coats form healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then blocked for 1 h with TBS-T 1% ECL Prime blocking reagent (GE Healthcare cat # RPN418) at RT on an orbital shaker and blotted with primary antibody for 1 h at RT with gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... Primary CD8+ and CD4+ T cells were isolated from buffy coats form healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was washed by PBS-T and then developed by Amersham ECL Western Blotting Detection Reagent (GE Healthcare) and imaged with AI600 Chemiluminescent Imager system (GE Life Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... three-dimensional T1-weighted anatomical MRI scans were acquired in a 1.5 T MRI system (GE Healthcare, Chicago, Illinois) using a high-resolution antenna and a homogenization PURE filter (fast spoiled gradient echo sequence ...
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bioRxiv - Neuroscience 2023Quote: Both structural and functional MRI data were obtained using a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI). High-resolution T1-weighted structural images were captured using a three-dimensional brain-volume sequence (TE = 3.18 ms ...
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bioRxiv - Genetics 2023Quote: ... Blots were washed 3 times with 1x TBS-T and incubated with anti-mouse HRP (GE Healthcare Cat # NXA931) or anti-rabbit HRP secondary antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... The radiolabeled primer was separated from the free nucleotides using the Microspin G-25 Columns (GE Healthcare) according to the manufacturer’s descriptions ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM EGTA) supplemented with 1 mM ATP to remove imidazole using PD-25 column (GE Healthcare). Drosophila KLP10A (plasmid pGG885 ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the purified protein were pooled and desalted with PD midi Trap G-25 (GE Healthcare) using the spin protocol ...
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bioRxiv - Neuroscience 2019Quote: ... The coupling reaction was filtered through a PD MiniTrap G-25 column (GE Healthcare #28-9180-07) to remove the excess of BG ...
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bioRxiv - Microbiology 2021Quote: ... and purified from unincorporated nucleotides by gel filtration on Illustra MicroSpin G-25 Columns (GE Healthcare, USA). Hybridisation was performed overnight at 50 °C in ExpressHyb Hybridisation Solution (Clontech ...
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bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
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bioRxiv - Microbiology 2021Quote: ... Unincorporated nucleotides were then removed by gel filtration on Illustra MicroSpin G-25 Columns (GE Healthcare, USA) and hybridisation was performed overnight at 40 °C in ExpressHyb Hybridisation Solution (Clontech ...
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bioRxiv - Cancer Biology 2020Quote: ... Fifty frames were acquired with a 25 μl bolus infusion of Gadolinium (Gd) solution (Omniscan GE HEALTHCARE) administered using a syringe pump (PHD2000 ...
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bioRxiv - Immunology 2022Quote: ... followed by Ni-NTA purification and desalting by PD MiniTrap G-25 columns (GE Healthcare Life Sciences).
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bioRxiv - Biophysics 2022Quote: ITC experiments were performed at 25°C by using a MicroCal VP titration calorimeter (MicroCal-GE Healthcare) under the same conditions as described previously (16) ...
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bioRxiv - Molecular Biology 2019Quote: ... After removal of unbound γ-ATP by MicroSpin G-25 column (GE Healthcare, Thermo Fisher Scientific Inc.) purification ...
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bioRxiv - Molecular Biology 2019Quote: ... All liquid chromatography steps were performed using ÄKTA Avant 25 system and chromatographic columns from GE Healthcare were used ...
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bioRxiv - Microbiology 2019Quote: ... Dual-labeled capsid proteins were separated using a PD Mini-Trap G-25 desalting column (GE healthcare) with imaging buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: All the surface preparation experiments were performed at 25°C using a Biacore 3000 instrument (GE Healthcare). Purified Rubisco was immobilized on CM5 sensor chips using a Biacore Amine Coupling Kit according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... each parasite culture was layered onto a 75/25% percoll (GE Healthcare Life Sciences, Pittsburgh, PA, USA) gradient ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The fluorescent polymersomes were purified using MidiTrap G-25 columns (GE Healthcare Europe, Freiburg i. Br., Germany) to remove the free dye and exchange the external phase with an isotonic sodium chloride-containing phosphate buffer (ammonia sensing ...
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bioRxiv - Microbiology 2019Quote: ... excess label was removed by size-exclusion chromatography using Sephadex G-25 columns (GE life sciences, USA). Labeled virus was used for visualization of internalized virus using confocal microscopy and correlative light and electron microscopy.
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bioRxiv - Biochemistry 2020Quote: The antibodies binding properties were analyzed at 25 °C on a Biacore ™ 8K instrument (GE Healthcare) using 10mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2021Quote: ... The binding experiments were performed at 25°C on a Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) in HBS-EP+ buffer (10 mM HEPES ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl) and captured by HiTrap MabSelectSure (Cytiva) 1mL column using ÄKTA Avant 25 (GE Healthcare). The column was then washed with 7.5 CV Buffer A ...
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bioRxiv - Microbiology 2022Quote: ... Imidazole was removed by passing the eluted protein fraction over a desalting NAPTM-25 column (GE Healthcare) equilibrated with buffer A and eluted with 2.5 ml of the same buffer ...
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bioRxiv - Plant Biology 2022Quote: ... crude extracts (4 ml) were passed through 0.5 ml DEAE-Sephadex A-25 columns (GE Healthcare, USA). The bound GSL was digested on the column for 18 h at room temperature with ∼0.8U myrosinase/thioglucosidase ...
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bioRxiv - Bioengineering 2023Quote: ... Three consecutives’ batches were performed in 5 L bioreactor using ReadyToProcess WAVE 25 system (GE Healthcare, USA). A single-use 10 L Cell bag™ bioreactor (working volume ...
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bioRxiv - Immunology 2022Quote: ... in 25 mM Tris pH 7.5 + 150 mM NaCl using an AKTA Pure FPLC instrument (GE Healthcare) to isolate the trimeric protein (and remove S2 pre-fusion protein for the Wuhan spike) ...
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bioRxiv - Biochemistry 2024Quote: ... size exclusion chromatography was followed by glycerol removal using a PD MiniTrap G-25 column (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 100-250 mM NaCl and 2 mM DTT using the ÄKTA™ Pure 25 system (GE Healthcare).
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bioRxiv - Plant Biology 2024Quote: ... Seeds were dried on sterile Whatman paper (ref: GE Healthcare Life Science, ME 25/31 ST, Whatman). 5-cm round plates were filled with 8 mL minimal medium (MES hydrate [M8250 ...
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bioRxiv - Cell Biology 2020Quote: ... diluted with 4 mL 0.5% Triton X-100 in PBS (PBS-T) and incubated with prepared glutathione sepharose beads (GE Healthcare) in a gravity column for 3 h at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
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bioRxiv - Neuroscience 2020Quote: MRI scanning was conducted on a 3.0 T GE MR750 whole-body 60cm bore MRI scanner (GE Healthcare, Waukesha, WI). Participants were sedated to reduce motion artifacts in the MRI ...
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bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Neuroscience 2022Quote: ... a computed tomography (CT) and a T1-weighted anatomical MRI scan on a 1.5-T Signa Excite scanner (GE Healthcare) were acquired ...
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bioRxiv - Bioengineering 2023Quote: ... 13 fetuses at 23w to 37w GA scanned on a 1.5 T GE scanner (Signa HDxt, GE Healthcare, United States) were used for the following analysis ...
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bioRxiv - Biochemistry 2024Quote: The binding affinity of b96.11 to apo- and holo- GAD65 was measured using surface plasmon resonance (BIAcore T-100, GE Healthcare). Purified monoclonal b96.11 IgG antibody was captured on a CM5 sensor chip with immobilised Protein G ...
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bioRxiv - Biophysics 2021Quote: ... The sample was then buffer exchanged using a Sephadex G-25 column (GE Healthcare Bio-Sciences, Pittsburgh, PA) to CD buffer (20 mM sodium phosphate ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples were further purified by gel filtration chromatography on an ÄKTA Pure 25 FPLC (GE Healthcare) using a Superdex75 or Superdex75 increase 10/300 GL column (GE Healthcare) ...