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Citations for GE Life Sciences :
4151 - 4200 of 4203 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (30 μg) were resolved by electrophoresis in a 10% or 12% gel and transferred to nitrocellulose membranes (GE Healthcare, Westborough, MA, #88018). The membranes were then incubated with specific antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were subjected to SDS–PAGE and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before(60) ...
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bioRxiv - Neuroscience 2023Quote: ... A small volume of the bead-coupled GST-fusion proteins was loaded into a ProbeQuant G-50 Micro Column (GE Healthcare, cat no. 28903408) and washed once in ice cold lysis buffer containing 1 % (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were subjected to SDS-PAGE (15 μg per lane) and blotted onto Protran Nitrocellulose Mem-brane-Whatman (GE Healthcare Life Science, USA). Membranes were blocked with Blocking Solution (5% powdered milk in TBST ...
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bioRxiv - Microbiology 2023Quote: ... aMCFCA was then purified from the supernatant using the AKTA protein purification system through a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02) with loading buffer (10 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and eluted with a linear gradient of imidazole (0–1,000 mM) in His-tag-binding buffer using the ÄKTA FPLC™ Fast Protein Liquid Chromatography system (GE Healthcare, Princeton, NJ, USA). The eluted protein peak was collected and dialyzed against buffer D containing 50 mM Tris–HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoreactive bands were detected by a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after incubation of the membranes with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analyses of the immunoreactive bands were performed by the FluorChem FC3 Analysis Software.
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was diluted to 100mM NaCl and clarified by centrifugation before application to a 5mL Heparin HiTrap HP (GE Healthcare 17-0407-01) column equilibrated with Buffer B (25mM HEPES pH 7.5 ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... DTX3L and Flag epitope for 1 h at 4°C before Protein G Sepharose beads (30 μL per reaction mixture; GE Healthcare #GE17-0618-01) were added for 12 h ...
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bioRxiv - Physiology 2023Quote: ... Immunoreactive bands were detected using a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after the membranes were incubated with ECL Prime Western Blotting Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analysis of the immunoreactive bands was performed using the FluorChem FC3 Analysis Software.
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were subjected to SDS–PAGE and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before[62] ...
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bioRxiv - Biophysics 2024Quote: ... Dyes were incubated with G-actin at a 2-fold molar excess for 2 hours at room temperature before being separated from the labeled protein by applying the labeling reaction to a spin column packed with Sephadex G-50 Fine DNA Grade (GE Healthcare GE17-0573-01) hydrated with A buffer (5 mM Tris-HCL (pH 8) ...
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bioRxiv - Biochemistry 2024Quote: ... and the flow-through sample containing the ASK1 TBD protein was purified by size-exclusion chromatography on a HiLoad 26/600 Superdex 75 pg column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM HEPES (pH 7.0) ...
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bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
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bioRxiv - Immunology 2019Quote: ... Cells were harvested and lysed in PBS supplemented with 1 mM PMSF and soluble protein was purified using nickel-affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare, Piscataway, New Jersey, USA). After SDS-PAGE analysis ...
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bioRxiv - Immunology 2019Quote: ... IgG and IgM antibodies were purified from plasma samples by affinity chromatography using prepacked HiTrap™ Protein G HP columns (GE Healthcare, Wauwatosa, WI, USA) and HiTrap™ IgM Purification HP columns (GE Healthcare ...
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bioRxiv - Systems Biology 2021Quote: ... Samples were centrifuged at 16,000 x g for 20 minutes and the supernatant used for affinity purification of biotinylated proteins using 25 µl of washed streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301) for 16 hours with rotation at 4℃ ...
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bioRxiv - Systems Biology 2021Quote: ... The lysate was boiled in Laemmli SDS-PAGE sample buffer for five minutes and 50 µg of protein per lane were separated onto 10% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science, Cat# 10600001). Following Ponceau S staining ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were centrifuged at 15,000 × g for 15 minutes and the supernatant was used for biotinylated protein capture using 15 μl of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... A tenth of the lysate was taken as TCE control and the rest was added to washed Protein G-coupled Sepharose beads (Sigma-Aldrich GE Healthcare 17-0618-01) for preclearing (30 min) ...
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Physiology 2020Quote: ... Immunoblotting was conducted with specific antibodies and protein bands were visualized using the enhanced chemiluminescence (ECL) Western Blotting Detection System (GE Healthcare, Chalfont St Giles, UK) or the Odyssey infrared detection system (LICOR).
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bioRxiv - Bioengineering 2021Quote: ... A second purification step was performed for IDO-Gal3 using size exclusion chromatography in an ÄKTA Pure Protein Purification System (GE Life Sciences, Marlborough, MA, USA). Molecular weight and purity were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Neuroscience 2021Quote: ... Control human blood samples were processed to remove the most abundant proteins using albumin and IgG depletion SpinTrap columns (GE Healthcare Cat 28-9480-20) following the manufacturers instructions ...
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bioRxiv - Pathology 2021Quote: ... 250 μg of total protein was loaded on a 13 cm Immobiline dry-strip (pH 4-7, M/s. GE Healthcare Life Sciences, Amersham, UK), on a rehydration tray ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were first purified with affinity chromatography using AKTA pure 25 system and HiTrap MabSelect SuRe protein A column (GE healthcare, Cat# 11-0034-94) for huACE2-IgHu-pVax ...
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bioRxiv - Biochemistry 2022Quote: Verification of the monomeric or dimeric state of those constructs was obtained by SEC-MALS experiments with the injection of 5 mg/mL of each protein into a Superdex 200 10/30 Increase column (GE Life Sciences, Velizy-Villacoublay, France) mounted on a HPLC Ultimate 3000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with developing solution kit (Amersham™ ECL Select™ Western Blotting Detection Reagent) and protein levels were detected using an ImageQuant LAS 500 (GE Healthcare Life Sciences). Images were processed and band intensities quantified using ImageJ ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were injected and purified on SEC (size exclusion chromatography) column Superdex 200 10/300 GL (GE Healthcare Life Sciences, Piscataway, NJ, USA) against a buffer containing 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: 1 mg lysate protein aliquots were used for immunoprecipitation with optimized amounts of the indicated antibodies and 20 μL of Protein A Sepharose beads (GE Healthcare, Chalfont St. Giles, UK) followed by SDS-Polyacrylamide (Biorad ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was further purified by size exclusion chromatography using HiLoad® 16/600 Superdex® 75 pg column (GE Life Sciences, 28-9893-33) equilibrated with 1x Tris buffer (50 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... The protein samples were then applied to size exclusion chromatography (Superdex 200 prep grad, HiLoad 26/60 or 16/60, GE Healthcare Life Sciences, Freiburg, Germany) using 50 mM HEPES-KOH (pH 7.2) ...
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bioRxiv - Plant Biology 2024Quote: ... His-tagged recombinant proteins were overexpressed in Escherichia coli strain Rosetta™ (DE3) and purified via an Äkta chromatography system using HisTrap columns (GE Healthcare, Waukesha, WI, USA). Washing (20 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... the oligomers were separated from the monomeric and fibrillar forms of the protein by size exclusion chromatography on a Superose 6 HR10/300 column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA) equilibrated in PBS pH 7.4 at a flow rate of 0.5 mL/min ...
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bioRxiv - Bioengineering 2020Quote: Experimental “affinity” between β-CD monomers and protein-conjugates were measured experimentally through surface plasmon resonance (SPR) with a Biacore X100 system (GE Healthcare Bio-Sciences, Pittsburgh, PA) according to previous protocols 31,32 ...
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bioRxiv - Biochemistry 2021Quote: Size exclusion chromatography (SEC) of the concentrated protein solution was conducted using a HiLoad™ 26/600 Superdex™ 200 pg column (GE Healthcare, Mu-nich, Germany) coupled to the aforementioned chromatography system ...
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bioRxiv - Cancer Biology 2019Quote: ... The immune complexes were mixed with 20 μl of Protein A or G sepharose 4 Fast flow beads (50% v/v, GE Healthcare Life Sciences, Marlborough, MA, USA). Whole cell lysates and immunoprecipitates were detected by Western blotting ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked and incubated with the antibody to detect neural Wiskott-Aldrich Syndrome protein (N-WASp; MilliporeSigma, Burlington, MA, USA) using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology) ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the purest form of the proteins were loaded on a size exclusion chromatography HiLoad® 16/60 Superdex® 200 pg column (GE Healthcare Life Sciences / Cytiva), pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA ...
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bioRxiv - Neuroscience 2023Quote: ... Separation of aSYN monomers and PFFs was performed with fast protein liquid chromatography using a Superose 6 10/300GL column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA). PFFs were briefly sonicated ...
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bioRxiv - Biophysics 2020Quote: Proteins were finally separated from free fluorophores by size exclusion chromatography with a PD MiniTrap G-25 prepacked columns (GE Healthcare Life Science, Chalfont St Giles, UK). Finally ...
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bioRxiv - Molecular Biology 2021Quote: ... The EGFP/YFP tags on the proteins was detected by applying SuperSignal® West Dura Extended Duration Chemiluminescent Substrate and imaging with an Amersham Imager 600RGB (GE Healthcare Life Sciences, Little Chalfont, UK).
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bioRxiv - Cell Biology 2024Quote: Cell or collagen lysates were separated by denaturing SDS-PAGE (6% gels for collagens, 10% for SMADs) and proteins blotted onto a nitrocellulose membrane (Amersham™, GE Healthcare, Chalfont St Giles, United Kingdom). Membranes were cut horizontally to detect proteins of different sizes ...
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bioRxiv - Biophysics 2020Quote: ... In the first step the ENTH-domain was isolated from the soluble cell lysate as GST fusion protein by using GSTrapFF™ 5 ml columns together with the ÄKTAPrime Plus system (GE Healthcare Life Science, Chalfont St Giles, UK). The cell lysate was injected onto the column (0.4 ml/min ...