Labshake search
Citations for GE Life Sciences :
4001 - 4050 of 4203 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Concentrated protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL (GE healthcare, GE28-9909-44) in SEC buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
-
bioRxiv - Biochemistry 2023Quote: ... The purified protein was concentrated and subjected to gel-filtration chromatography using a Superdex 200 increase 10/300 column (GE Healthcare, USA), pre-equilibrated with gel-filtration buffer containing 25 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Immuno-reactive protein bands were detected by the ECL system and visualized by imaging analyzer (ImageQuant LAS 500, GE Healthcare Life Sciences).
-
bioRxiv - Biophysics 2023Quote: ... The processed proteins were purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL). Purified ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein extracts were resolved on SDS-PAGE (10% acrylamide) and transferred to a nitrocellulose Hybond ECL membrane (GE Healthcare, Pittsburgh, PA, USA) for probing with high-affinity rat monoclonal antibodies anti-HA (Roche ...
-
bioRxiv - Biochemistry 2024Quote: ... The purification of Ubi-SSFc and His6-SSFc was performed using HisTrap resin and AKTA protein purification system (Ge Healthcare Life Sciences). Firstly ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS– PAGE) and proteins were transferred into nitrocellulose membranes (Amersham Protran Premium 0.45μm nitrocellulose blotting membranes, GE Healthcare Life Sciences, USA). For immunostaining ...
-
bioRxiv - Cell Biology 2024Quote: ... the protein signal was developed by Super Signal West Femto (Thermo 34095) and visualized with Amercham Hyperfilm ECL system (GE Healthcare 28906839) film.
-
bioRxiv - Microbiology 2020Quote: ... The lysates were centrifuged at 13,000 RPM for 5 minutes at 4°C and the supernatant was transferred to a new tube and cleared of residual IgG by rotation with Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for 30 minutes at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... The purifications of these proteins from transformed bacterial cell extract were performed according to manufacturer instructions (GST bulk kit, GE Healthcare Life Sciences). The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences) ...
-
bioRxiv - Cell Biology 2020Quote: ... was purified using an ÄKTAprime plus fast protein liquid chromatography (FPLC) system equipped with a 1-mL His-Trap HP column (GE Healthcare-Amersham Biosciences) and the high salt buffer ...
-
bioRxiv - Immunology 2021Quote: ... After washing the membranes with TBST, protein bands were detected with a chemiluminescent substrate (BIOMAX, USA) through a CCD Imager (GE life sciences, USA).
-
bioRxiv - Molecular Biology 2020Quote: ... Elution buffer was exchanged to standard Tris protein buffer (100 mM Tris and 150 mM NaCl at pH 7.5) with NAP-5 columns (GE Healthcare, Chicago, IL, USA) and samples were concentrated as before.
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified from the infected cell culture supernatants by binding to a HiTrap ProteinG HP column (GE Healthcare Life Sciences, Piscataway, NJ) and eluted with 0.1 M citrate ...
-
bioRxiv - Biophysics 2022Quote: ... After purification with Ni-NTA agarose the protein was diluted to 375mM NaCl and further purified on an ÄKTA purifier system (GE Healthcare Life Sciences) at 4 °C using 1ml HiTrap™ Heparin HP column (Cytiva) ...
-
bioRxiv - Microbiology 2019Quote: ... YpdAC14A and Tpx-roGFP2 proteins were purified using His Trap™ HP Ni-NTA columns (5 ml; GE Healthcare, Chalfont St Giles, UK) and the ÄKTA purifier liquid chromatography system (GE Healthcare ...
-
bioRxiv - Plant Biology 2020Quote: Protein extracts (150 mg per sample) were incubated with 300 µl of IgG-Sepharose 6 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h at 4°C with gentle rotation (1000 rpm) ...
-
bioRxiv - Developmental Biology 2019Quote: ... 30 μg of proteins from lysates were subjected to migration on 8–12 % acrylamide gels and transferred on to polyvinylidene difluoride membranes (GE Healthcare Europe GmbH) in borate buffer (50 mM Tris-HCl and 50 mM borate ...
-
bioRxiv - Biophysics 2019Quote: ... for 20 min at 4 0C and histidine tagged protein was recovered from the lysate using a HiTrap TALON Crude Co2+ column (GE Healthcare, Piscataway, NJ) as described by the manufacturer ...
-
bioRxiv - Neuroscience 2019Quote: ... P2 fractions were pre-cleared in NP-40 buffer for 4 hours and then incubated o/n at 4 °C with protein A-Sepharose beads (GE Healthcare, Milan, Italy) conjugated to 10 μg/ml of anti-Pin1 antibody (Santa Cruz Biotechnology ...
-
bioRxiv - Systems Biology 2019Quote: ... Lysates (10-40 μg/lane) were separated by SDS-PAGE and protein was transferred onto a polyvinylidene fluoride (PVDF) membrane (Hybond-P, GE Healthcare, Uppsala, Sweden). Specific primary antibodies (see Antibodies subsection ...
-
bioRxiv - Immunology 2021Quote: Total IgG was initially purified from 4 mL of the multigravidae plasma pool using Protein G Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... The proteins were further purified according to their size on a HiLoad™ 16/60 Superdex™ 200 prep grade prepacked column (GE Healthcare) using an ÄKTAprime chromatography system at 4°C with a SEC buffer containing 50 mM Tris/HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... Selected fractions containing the N protein were concentrated and loaded on two connected Superdex 200 10/300 GL size exclusion columns (GE Healthcare Life Sciences) pre-equilibrated with 20 mM HEPES ...
-
bioRxiv - Biochemistry 2020Quote: ... a single 0.5 ml aliquot of the final protein was thawed and analyzed by analytical size-exclusion chromatography using a 10/300 Superdex200 analytical column (GE Healthcare, Chicago, IL), with a flow rate of 0.5 ml/min.
-
Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We pooled fractions containing the purified protein, desalted the pooled fractions using PD-10 desalting columns packed with Sephadex G-25 resin (17085101, Cytiva (formerly GE Healthcare Life Sciences), Marlborough ...
-
bioRxiv - Biophysics 2020Quote: ... SHP-1 was further purified by size-exclusion chromatography on an AKTA fast protein liquid chromatography system equipped with a Superdex S200 10/300 GL column (both from GE Healthcare Life Sciences) equilibrated with 10 mM Hepes (pH 7.4) ...
-
bioRxiv - Biochemistry 2021Quote: Extracted proteins of three-day-old sexual fungal tissues were separated by PAGE and transferred onto a nitrocellulose membrane (GE Healthcare, Wisconsin, USA) as described (Schinke et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... and 25μg of whole protein lysate were resolved on SDS-PAGE gels prior to transfer to PVDF membranes (GE Healthcare Life Sciences, #10600023) using a tank blotting apparatus (BioRad).
-
bioRxiv - Neuroscience 2020Quote: ... The remaining elution buffer from the protein purification process was substituted by PBS using sephadex-based PD MidiTrap G-25 columns (GE Healthcare, Chicago, USA). Afterwards ...
-
bioRxiv - Neuroscience 2019Quote: ... and 140 mM NaCl) was added and samples were pre-cleared for 1 hour using protein G sepharose (GE Healthcare, 17-0618-01). Immunoprecipitation was carried out overnight at 4 °C using anti-HA agarose beads (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... Culture supernatants from both approaches were filtered and mAbs were purified from culture supernatants using HiTrap protein G columns (GE Healthcare Life Sciences) according to the manufacturer’s protocol.
-
C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: Gel filtration was performed using fast protein liquid chromatography (AKTA-FPLC system) on a Superose 6 10/300 GL column (GE Healthcare Life Sciences) as described previously (Hořejší et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... They were then incubated with a horseradish peroxidase-conjugated secondary antibody and proteins were detected using enhanced chemiluminescence (GE Healthcare Life Sciences, RPN2209). Densitometry analysis of bands was performed using Image J software.
-
bioRxiv - Biophysics 2022Quote: ... Purification of resulting protein was performed by immobilized metal affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare, Frei-burg, Germany) containing HisTrap chelating stationary phase (Amersham Biosciences ...
-
bioRxiv - Biophysics 2022Quote: ... was titrated into an A1 protein solution (22 μM except 50 μM with A1+OGly+N+C) in a MicroCal iTC200 (GE Healthcare Life Sciences). A priming injection of 0.4 μL (not included in data analysis ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size-exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 20 mM HEPES pH 7.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size- exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 50 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
-
bioRxiv - Microbiology 2022Quote: ... the membrane was incubated with a self-made developing solution and protein levels were detected using an ImageQuant LAS 500 (GE Healthcare Life Sciences). Images were processed and band intensities quantified using ImageJ (37).
-
bioRxiv - Biochemistry 2022Quote: ... 1 mg of each of the remaining lysates was diluted four-fold with IP buffer plus 0.1% v/v Triton X-100 and incubated for 2 hr at 4 °C with protein G-Sepharose (GE Healthcare catalog no. 17061801), pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...
-
Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Bacterial lysates were cleared by centrifugation at 10,000 g for 20 min at 4°C and applied to a HisTrap HP column using an AKTA Prime protein purification system (GE Healthcare, Life Sciences). Bound protein was eluted with a linear imidazole gradient ...
-
bioRxiv - Cancer Biology 2022Quote: ... cell lysates were immunoprecipitated with the indicated antibodies for 3 h at 4 °C and then incubated with protein G (GE Healthcare Life Sciences) for 2 hrs at 4 °C ...
-
bioRxiv - Immunology 2020Quote: ... Cell pellets were resuspended in 750 µL PBS and mixed thoroughly with 50 µL Protein A/G Sera-Mag SpeedBeads (GE Life Sciences, 17152104010350) (6.25 % the beads’ stock concentration) ...
-
bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using 0.45 micrometer membranes (PES, ClearLine) and the protein purification was carried out by IMAC using 1 mL His-Trap FF columns (GE Healthcare Life Sciences) and a NGC chromatography system (Bio-Rad) ...
-
bioRxiv - Immunology 2019Quote: Protein samples were subjected to 10% SDS-PAGE and the proteins were then electrophoretically transferred to Hybond-ECL nitrocellulose membranes (GE Healthcare, Amersham, UK). After blocking with 1% bovine serum albumin (BSA ...
-
bioRxiv - Biochemistry 2020Quote: ... These affinity-purified protein concentrates were then applied to a previously equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare Life Sciences) packed with dextran covalently bound to highly cross-linked agarose ...