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Citations for GE Life Sciences :
3801 - 3850 of 4203 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Protein purification was conducted using a combination of affinity and size-exclusion chromatography (SEC) performed using Äkta Purification systems (GE Healthcare). Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: Further protein purification was achieved by size exclusion chromatography (SEC) through a Superdex™ 200 10/300 GL column (GE Healthcare) using SEC buffer containing 0.5 M NaCl and 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Tev protease and other contaminants were removed with Nickel NTA resin and the unbound protein purified further using a HiLoad 16/60 superdex 75 size exclusion column (GE Healthcare). His-GST-DIDATRX ...
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bioRxiv - Biochemistry 2022Quote: ... To insure the monodispersity of samples all proteins were subjected to SEC directly before collecting of scattering data on a Superdex 200 10/300 GL (GE Healthcare) equilibrated with 20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... and the resulting compounds were purified from cell culture supernatants using an kta Express chromatography system using affinity purification using Mabselect Sure Protein A resin followed by a gel filtration column based on the Superdex200 resin (GE Healthcare). The MabSelect Sure Protein A column was washed with 0.1 M Hepes ...
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bioRxiv - Biophysics 2022Quote: ... Size exclusion chromatography was performed on the concentrated protein using a Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) equilibrated in gel filtration buffer (10 mM Na2HPO4.7H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Biophysics 2023Quote: ... lysed with a high-pressure homogenizer and Fab was purified with a 5-ml HiTrap Protein G HP column (GE Healthcare). Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0 ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated using SDS-PAGE in 10 % Bis-Tris acrylamide/ bisacrylamide gels and transferred to a nitrocellulose membrane (GE Healthcare). The membranes were blocked for 30 minutes with PBS 5 % milk and incubated with the first antibody for 1 hour at room temperature or at 4°C over-night with gentle shaking ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was subjected to size-exclusion chromatography using HiLoad Superdex 200 prep-grade 16/600 column (GE Healthcare Biosciences) pre-equilibrated with buffer F (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Proteins separated based on molecular weight onto the gel were transferred onto a 0.2 μm western blot membrane (GE life sciences) using a thermo scientific transfer cassette for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 35 μg protein from each sample was resolved by SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). The following antibodies were used at the indicated dilutions ...
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bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...
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bioRxiv - Bioengineering 2023Quote: ... Equal Volume of total soluble proteins were separated on a 15% (w/v) SDS polyacrylamide gel and transferred onto a Hybond-P membrane (RPN303F, GE Healthcare). Nonspecific binding was blocked with 3% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... pH 7.4 400 mM Imidazole) The labeled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex-75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove other contaminants and soluble aggregates ...
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bioRxiv - Biophysics 2023Quote: ... the protein was diluted 10 times with PBS buffer and immobilized on a Nickel Sepharose 6 Fast Flow resin (GE Healthcare). The resin was washed extensively with milliQ water followed by PBS buffer pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated and was further purified by a Superdex increase 200 16/600 size exclusion chromatography column (GE Healthcare) that was pre-equilibrated with SEC buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The following morning unbound G protein and Nb35 were separated from the complex by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with the buffer containing 20 mM HEPES/KOH pH7.5 ...
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bioRxiv - Immunology 2023Quote: ... The membrane was then washed several times in 0.05% TBS-T and proteins were detected using enhanced chemiluminescence (ECL) reagent (GE Healthcare; RPN2106). Antibodies used include ICAM-1 (Cell Signaling Technologies ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Biophysics 2023Quote: ... The cleaved protein of interest was then purified by size exclusion chromatography using a Superdex 75 prep grade column (GE Healthcare). Where necessary ...
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bioRxiv - Biochemistry 2023Quote: ... the His-MBP tag was cleaved off by TEV protease and the protein was further purified by a passage through a Ni2+-Chelating Sepharose (GE Healthcare). IHO1 was then purified by a gel filtration on Superdex 200 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... and the soluble His6-M protein was purified on a Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Biophysics 2023Quote: Ion exchange chromatography (IEC) was performed using an ÄKTA Purifier protein purification system controlled by the software Unicorn 3.10 (GE Healthcare, Sweden). CIMmultus QA 1 mL column (BIA Separations ...
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bioRxiv - Biophysics 2023Quote: ... The protein eluate was concentrated and further purified by size-exclusion chromatography on a Superose6 10/300 GL column (GE Healthcare). Peak fractions were collected for functional assay.
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bioRxiv - Immunology 2022Quote: ... Samples were resolved on 12–15% SDS–PAGE gels and then proteins were wet transferred to Protran nitrocellulose membranes (GE Healthcare) at 400 mA at 4 °C for 1.5 h in 1x Transfer buffer (25 mM Tris base ...
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bioRxiv - Cell Biology 2022Quote: Cellular lysates were separated on a Superose 6 HR 10/30 size-exclusion column and an AKTA Purifier protein purification system (GE Healthcare), essentially as described previously(61) ...
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bioRxiv - Cell Biology 2022Quote: ... The tag-free protein was further purified by size-exclusion chromatography on a Hiload 16/60 Superdex 75 column (GE Healthcare). Mutants and ZF truncations were expressed and purified using the same procedure as for the wild-type proteins ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was concentrated and subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare, 289909944) that was pre-equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The biobeads were removed by filtration and the protein-amphipol mixture was concentrated before purification by size exclusion chromatography using a Superdex S200 column (GE Healthcare) pre-equilibrated with buffer SEC2 (200 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then washed in PBS-T and incubated with 1 mL of Enhanced ChemiLuminescence (ECL) (Amersham) reagent for 5 minutes to allow protein visualization on an x-ray Amersham Hyperfilm ECL (GE Healthcare). Fiji-Image J Software was used for quantification of the difference in protein expression comparing with GAPDH or HSP70 expression.
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bioRxiv - Biophysics 2023Quote: ... The concentrated protein sample was further purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL (GE Healthcare) column pre-equilibrated with the buffer E (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: GST fusion protein was similarly purified using affinity chromatography by incubating bacterial cell lysate with glutathione-Sepharose 4B beads (#17075601; GE Healthcare) according to the manufacturer’s instructions and eluted from beads with elution buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... TCA extracts of total yeast or 2 μg of mitochondrial proteins were loaded on a 12 % SDS-PAGE and transferred onto a nitrocellulose membrane (GE Healthcare). UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD) ...
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bioRxiv - Immunology 2023Quote: ... Protein-containing fractions were combined and purified further via SEC on a Superdex 75 10/300 GL column (GE Healthcare/Cytiva) using 20 mM sodium phosphate buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... was used as the secondary antibody. Protein was detected using the chemiluminescent substrate ECL (Cat. # 32209, Pierce) in an Amersham Imager 680 (GE Healthcare) detector.
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were then incubated over night at 4 °C with appropriate antibodies (1ug/ml) and protein A Sepharose beads (GE Healthcare). After incubation samples were washed five times with lysis buffer and resuspended in 1X Laemmli sample buffer (Bio-Rad cat ...
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bioRxiv - Biophysics 2023Quote: ... the protein was subjected to ion exchange chromatography and loaded onto a HiLoad 26/10 Q Sepharose high performance column (GE Healthcare), and eluted at ∼350 mM NaCl with a salt gradient from 0 M to 1.5 M NaCl in buffers containing 25 mM Tris-HCl (pH 7.7) ...
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bioRxiv - Biochemistry 2023Quote: ... After His-tag purification protein was subsequently purified by size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare). The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by gel filtration using fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superdex 200 Increase 10/300 column (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was again washed three times with TBST and the protein of interest was detected using a chemiluminescence reagent (ECL Prime, GE Healthcare). Chemiluminescence was detected using the ImageQuant LAS500 imager (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... insoluble material was removed by centrifugation at 10,000 × g and the cell lysate was pre- cleared by addition of heat inactivated goat serum and protein G sepharose beads (GE Healthcare). Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1 ...
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bioRxiv - Cell Biology 2023Quote: ... Reduced immunoprecipitated proteins were separated on a 10 or 12% SDS-polyacrylamide gel and transferred to a PVDF membrane (GE Healthcare). Details on the blocking of the membranes and detection of the proteins can be found in Table S1 ...
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bioRxiv - Immunology 2022Quote: ... Proteins in gels were either stained with Coomassie blue dye in gel or used for transfer onto nitrocellulose membranes (GE Healthcare). The membranes were blocked with 5% milk in PBST (PBS and 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2023Quote: ... The protein elutes in the flow through and is subsequently subjected to size exclusion chromatography using a Superdex 200 (GE Healthcare) in a buffer containing 50 mM HEPES (pH 8.2) ...
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bioRxiv - Microbiology 2023Quote: ... and the protein was eluted with an imidazole gradient (300 mM-500 mM) using the AKTA prime chromatography system (GE Healthcare). Fractions containing pure ParR protein (26.5 kDa ...
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bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...