Labshake search
Citations for GE Life Sciences :
4101 - 4150 of 4868 citations for Glutathione Colorimetric Detection Kit 200 High Cuvettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... All SpyCEP constructs were subsequently concentrated and purified by size exclusion chromatography on a HiLoad Superdex 200 or 75 prep grade column (GE Healthcare) dependent on molecular weight.
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bioRxiv - Biochemistry 2023Quote: ... non-farnesylated constructs of GBP1 were purified by affinity chromatography (Ni Sepharose HP, Cytiva) and size exclusion chromatography (Superdex 200 16/600, GE Healthcare). Farnesylated GBP1 constructs were co-expressed with recombinant farnesyltransferase (FTase ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated at room temperature for 4 hours and was purified using gel filtration chromatography (ÄKTA with Superdex-200 increase column; GE Healthcare) equilibrated with storage buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated and was further purified by a Superdex increase 200 16/600 size exclusion chromatography column (GE Healthcare) that was pre-equilibrated with SEC buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The following morning unbound G protein and Nb35 were separated from the complex by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with the buffer containing 20 mM HEPES/KOH pH7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Images were acquired with 200 ms exposure time every 6 minutes for at least 6 h at 30°C using Softworx (Applied Precision) software ...
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bioRxiv - Biochemistry 2023Quote: PrgB188-1233 dimer fractions were thawed on ice and loaded on a Superdex 200 10/300 GL gel filtration column (GE Healthcare) equilibrated in 20 mM HEPES/NaOH pH 7.0 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: SUV420H1-H2A.Z nucleosome complex was assembled in a ratio of 3:1 and was purified on HiLoad Superdex 200 10/300 (GE Healthcare) size-exclusion liquid chromatography column (Buffer ...
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bioRxiv - Biochemistry 2022Quote: ... NSs::6xHIS was concentrated using a 10 kDa MWCO spin concentrator (Amicon) and further purified by size exclusion chromatography over a Superdex 200 Increase 10/300 GL column (GE Healthcare) in Elution Buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... Q7 or Q20 at a concentration of ~4-7mg/ml were loaded onto an S200 Increase size-exclusion column (Superdex 200 Increase10/300 GL, GE Healthcare) at a flow rate of 0.5 ml min-1 ...
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bioRxiv - Biochemistry 2022Quote: ... NSs::6xHIS was concentrated using a 10 kDa MWCO spin concentrator (Amicon) and further purified by size exclusion chromatography over a Superdex 200 Increase 10/300 GL column (GE Healthcare) in Elution Buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... was purified using a nickel-NTA agarose affinity column followed by a size-exclusion chromatography (Superdex 200 or Superdex 75, GE Healthcare) with a column buffer containing 50 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... the protein was loaded through a Ni-NTA column before a size-exclusion chromatography using Superdex 200 increase 10/300 GL column (GE Healthcare) in G-buffer ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was concentrated and subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare, 289909944) that was pre-equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... concentrated using a 30 kDa spin concentrator (Amicon) and subjected to size-exclusion chromatography using a Superdex 200 increase 10/300 GL column (GE Healthcare) in a buffer 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... All BtuF constructs were purified directly from whole cell lysate (obtained by sonication) via Ni-NTA chromatography followed by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) in final buffers of 20-50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2022Quote: Primary human AML specimens and normal MPB samples were resuspended at about 100-200 e6 cells per ml in freezing media composed of 50% FBS (GE Healthcare), 10% DMSO (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... followed by removal of the N-terminal His6-Ztag by TEV protease cleavage and size exclusion chromatography using a Superdex 200 16/60 column (GE Healthcare) (Roostalu et al ...
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bioRxiv - Cell Biology 2023Quote: SEC-MALS experiments were carried out on DAWN HELEOS-II (Wyatt Technology Corporation) coupled with a Shimadzu liquid chromatography system equipped with a Superdex 200 10/300 GL (GE Healthcare) gel-filtration column ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were eluted with HBS containing 200 mM imidazole and further purified using size-exclusion chromatography (Superdex 200 Increase 10/300 or Superose 6 Increase 10/300 columns; GE Healthcare) in 10 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: GB1-FKBP-ICD and GB1-FRB-ICD were mixed at equimolar ratio and loaded onto a 24-mL Superdex 200 column (GE healthcare) equilibrated in 100 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... Cleaved GST-tag and bZip construct were separated by gel filtration chromatography using Hi-Load 16/600 Superdex 200 pg (GE Healthcare) on AKTA pure in a 20 mM MES pH 6.0 ...
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bioRxiv - Plant Biology 2023Quote: ... The cleaved AVR proteins were then eluted and further purified by size-exclusion chromatography using a HiLoad 16/600 Superdex 200 pg gel filtration column (GE Healthcare). Purified proteins were concentrated to 10–30 mg/ml by using a 10-kDa Amicon centrifugal filter device (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: ... Recipient mice were allowed to recover for 6 weeks post-transplantation before Cre-LoxP recombination was induced by two intraperitoneal injections of Poly(I)-Poly(C) (300 μg in 200 μl PBS; #27-4732-01 GE Healthcare) with 48 h rest in-between ...
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bioRxiv - Synthetic Biology 2023Quote: Coupled PEG-PhyB and PEG-PIF6 were purified by size exclusion chromatography using a HiLoad Superdex 200 pg (16/600 or 25/600, GE Healthcare) column connected to an ÄKTA Explorer system with PBS as a running buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were eluted with a lysis buffer containing 400 mM imidazole and further purified with size exclusion chromatography (Superdex 200 column, GE Healthcare) with a buffer containing 50□mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... was then centrifuged at 21,000 ×g at 4 °C for 10 min and 100 µL of the supernatant was loaded on to a Superdex 200 column (25 mL, GE Healthcare) equilibrated with the Buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... it was purified using nickel affinity chromatography and finally subjected to size exclusion chromatography on a Superdex 200 10/300 gel filtration column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... Sample was allowed to complex overnight (∼18 hours) at room temperature and were SEC purified on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) using TBS as a running buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The protein elutes in the flow through and is subsequently subjected to size exclusion chromatography using a Superdex 200 (GE Healthcare) in a buffer containing 50 mM HEPES (pH 8.2) ...
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bioRxiv - Biochemistry 2023Quote: ... The untagged sF or sG was further purified with size-exclusion column (HiLoad 16/60 Superdex 200 prep grade XK 16 gel filtration column (GE Healthcare). The buffer PBS was used for sG and the PBS with 0.01% Triton X-100 was used for sF purification.
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bioRxiv - Immunology 2023Quote: ... protein was purified using cell-free culture supernatant by StrepTactin resin (IBA) and by size-exclusion chromatography using Superdex 200 (RBD and NTD) or Superose 6 (S-2P) column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Purification of His-tagged domains was achieved by nickel-affinity using purification buffer supplemented with 1 mM DTT and gel filtration chromatography in 50mM ammonium bicarbonate pH 7 using a Superdex 200 Increase 10/300 column (GE Healthcare) following published protocols67 ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... aVBD was then eluted in PBS and further subjected to purification using size exclusion chromatography using a Superdex 200 10/300 GL (Ge Healthcare). Samples were stored aliquoted at -80°C at concentrations ranging from 1 to 10 mg/ml.
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... 200μl of the protein mixtures were analyzed by size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare) using a GE ÄKTA FPLC™ (Fast Protein Liquid Chromatograph ...
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bioRxiv - Neuroscience 2023Quote: ... 3RCF and 4RCF tau proteins were further purified by FPLC using size exclusion Superdex-75 and Superdex-200 columns (GE Healthcare) in 1 x PNE buffer (25 mM PIPES ...
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bioRxiv - Microbiology 2023Quote: ... The fractions with high purity were concentrated using 10 kDa cutoff Amicon filters and then further purified by FPLC using a HiLoadTM 16/600 SuperdexTM 200 pg column (GE Healthcare) equilibrated with sizing buffer (500 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... with a 10 kDa cut-off and loaded onto a Superdex 200 pg HiLoad 16/600 (GE Healthcare, 1 mL/min). Sample purity was assessed by 12% SDS-PAGE analysis and sample concentration was determined by spectrophotometry using a theoretical molar extinction coefficient at 280 nm of 20 ...
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bioRxiv - Molecular Biology 2023Quote: ... aureus EF-G was purified further by gel filtration on a Hiload 16/60 Superdex-200 column (GE Healthcare, Uppsala, Sweden) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were pooled and further purified by gel filtration with a Superdex 200 increase 10/300 GL column (GE Healthcare). Peak fractions were pooled and concentrated to 11 mg/ml.
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bioRxiv - Biochemistry 2023Quote: ... Cleaved BRCA2 Ex14 was further purified by HiTrapSP HP ion exchange chromatography and size exclusion chromatography (HiLoadTM 16/600 Superdex 200, GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Desired fractions were incubated with 0.5 mM FeCl3 in presence of 200 mM NaHCO3 for 1h at room temperature and further purified SEC on a S200 16/600 column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved DMC1 was further purified by HiTrapQ HP ion exchange chromatography and size exclusion chromatography (HiLoadTM 16/600 Superdex 200, GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... with a 10 kDa MWCO before being subjected to size exclusion chromatography (SEC) on a Superdex 200 16/60 column (GE Healthcare) connected to an Äkta FPLC system (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... LmdC was further purified by size exclusion chromatography (SEC) on a HighLoad 16/60 Superdex 200 pg column (GE Healthcare, USA) equilibrated with SEC buffer (20 mM Tris/HCl pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing the protein of interest were concentrated and further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... All proteins were further purified by gel filtration on preparative Superdex HiLoad 26/600 or analytical (Superdex 200 10/300) columns (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... stored in sample loops on the system and injected into a Gel Filtration column (HiLoad 16/60 Superdex 200 prep grade, GE Healthcare). The protein sample was concentrated using Vivaspin 20 filter concentrators (VivaScience ...
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bioRxiv - Cell Biology 2023Quote: ... Rab35BD and Rac1BD-Rab35BD) (40 µL; 10-25 mg/ml) were loaded onto a Superdex-200 increase 5/150 column (GE Healthcare) or BioSec5 300 (Agilent ...