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Citations for GE Life Sciences :
301 - 350 of 6415 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Unincorporated biotin and aggregated viruses were removed by NAP-5 gel filtration columns (GE Healthcare) and 0.2 μm pore size filters ...
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bioRxiv - Microbiology 2021Quote: ... Biotin excess was removed by SEC on a Superdex 200 10/300 column (GE Healthcare). In the SEC profile ...
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bioRxiv - Plant Biology 2022Quote: Supernatants were collected and dialyzed to remove free biotin with PD-10 columns (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... and incubated overnight with primary antibodies (aFLAG 1:2,500, aHSP90 1:50,000, GE Healthcare) containing 0.01% Sodium azide (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
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bioRxiv - Microbiology 2020Quote: ... The protein was then loaded into a 1-mL HisTrap column (GE Healthcare) that had been equilibrated with buffer A [100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... The protein was subsequently applied to a 1 mL StrepTactin column (GE Healthcare) yielding pure αMβ2 headpiece ...
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bioRxiv - Microbiology 2021Quote: ... The protein was then loaded into a 1-mL HisTrap column (GE Healthcare) that had been pre-equilibrated with buffer A [100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were loaded on a HiTrap Heparin HP column (1 mL, GE healthcare) equilibrated with buffer C ...
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bioRxiv - Immunology 2022Quote: ... through a 1 ml HiTrap Protein G HP column (GE Healthcare Life Sciences) previously equilibrated with Loading Buffer (0.02 M NaH2PO4 pH 7) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each protein was purified using a 1 mL HisTrap HP column (GE Healthcare), washing with 10 mM Hepes-NaOH ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were purified using a 1 ml HisTrap HP column (GE Healthcare) or 5 ml GSTrap HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Msh5 ChIP was performed using polyclonal Msh5 antibody (generated in rabbit) and Protein A Sepharose beads (GE Healthcare) on synchronized meiotic cultures as described in Krishnaprasad et al ...
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bioRxiv - Microbiology 2019Quote: ... Anti-CFP polyclonal antibodies were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare) according to manufacturer instructions.
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bioRxiv - Immunology 2022Quote: IgG was purified by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences, 17040203) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Immunology 2021Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatant using a HiTrap protein A column (Cytiva, GE Healthcare). IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated with mouse monoclonal anti-MYC-antibody (Covance) and protein G-agarose beads (GE Healthcare). The precipitates were then washed with a lysis buffer ...
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bioRxiv - Bioengineering 2020Quote: ... Transmembrane stem cell factor (tmSCF) was then isolated using cobalt chelating column (Chelating column; GE Healthcare), and the buffer was exchanged in 1X PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Free iodine and iodinated growth factor were separated on Sephadex G-25 columns (PD10; GE Healthcare). For column equilibrium and elution ...
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bioRxiv - Biochemistry 2023Quote: ... Antigen B was further purified from Fc using rProteinA Sepharose (GE Healthcare), followed by deglycosylation with endoH (New England Biolabs) ...
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bioRxiv - Cell Biology 2019Quote: ... and horseradish peroxidase-conjugated secondary antibodies (1:10000, GE Healthcare). Protein bands were visualised using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermofisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies: anti-rabbit (1:10,000, GE Healthcare, Cat. #NA934V); anti-mouse (1:10,000 ...
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bioRxiv - Cell Biology 2022Quote: Goat GST antibody (27457701V, GE Healthcare, used at 1:1,000), NeutrAvidin-DyLight 800 (22853 ...
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bioRxiv - Genetics 2020Quote: ... and 1:10,000 anti-rabbit-HRP antibody (GE Healthcare, NA934V). RFP corresponds to the lin-48p::mRFP co-injection marker present in all transgenes ...
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bioRxiv - Plant Biology 2023Quote: ... anti-rabbit secondary antibody from GE Healthcare (1:10,000 dilution), bands were detected by chemiluminescence using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and incubated overnight with aFLAG antibody 1:2,500 (GE Healthcare) on a gently rocking platform at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Both kinetic and equilibrium binding data were analysed in Biacore 8K evaluation software version 1.1 (GE Healthcare). Equilibrium binding measurements were taken once equilibrium had been reached using reference-subtracted sensorgrams ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
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bioRxiv - Developmental Biology 2019Quote: The single cycle kinetics method was used for sFLT1 binding assay by Biacore (GE Healthcare Bio-Science). A CM5 series S sensor chip (GE Healthcare Bio-Science ...
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bioRxiv - Biophysics 2020Quote: Calorimetric binding experiments of Tim23IMS and TIM chaperones were performed using a MicroCal ITC200 instrument (GE Healthcare). Sixteen successive 2.5 µl aliquots of 1.15 mM Tim23IMS were injected into a sample cell containing 55 µM TIM9·10 or TIM8·13 ...
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bioRxiv - Immunology 2021Quote: ... The binding experiments were performed at 25°C on a Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) in HBS-EP+ buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: The binding kinetics of FNBP4 and FMN1 was determined by SPR BIAcore T200 (GE Healthcare Life sciences). The surface of the CM5 sensor chip (Series S ...
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bioRxiv - Neuroscience 2023Quote: The binding of FUS to (G4C2)4 RNA was analyzed using a Biacore T200 instrument (GE Healthcare). (G4C2)4 RNAs biotinylated at the 5′ end in 10 mM HEPES pH 6.8 and 500 mM MCl (M = K ...
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bioRxiv - Immunology 2021Quote: ... Biotin excess was removed by SEC on either a Superdex 200 10/300 column (GE Healthcare) for the RBD probe or a HiLoad Superose 6 16/600 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and unincorporated biotin-17-dCTP was removed by using S-200 HR Microspin columns (GE Healthcare). The DNA was ethanol precipitated and resuspended in TE buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Biotin-labelled PCR amplicons were captured by mixing PCR products with streptavidin-sepharose beads (GE Healthcare). Vacuum Prep Tool with filter probes was used to capture sepharose beads with biotin labelled PCR amplicons ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-labelled HCV 3’UTR RNA was immobilized on streptavidin-coated sensor chips (GE Healthcare Lifescience) to a final concentration of 300 Resonance Units (RU)/flow cell ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM DTT at pH 7.0) while incubating with PreScission protease (protease to protein ratio 1:2000) (GE Healthcare) overnight ...
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bioRxiv - Microbiology 2022Quote: ... antibodies diluted 1:500 in 1 U/mL DNAse I in Tris Buffered Saline containing 1% BSA (GE Healthcare). Three 5-minute washes with block (1% BSA with 0.1% Tween-20 ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged mammalian ATG8 fusion proteins were cloned into pGEX-4T-1 (GE Healthcare) and expressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced by adding (isopropyl β-D-1-thiogalactopyranoside, IPTG; GE Healthcare) to a final concentration of 0.3 mM ...
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bioRxiv - Microbiology 2021Quote: ... the protein was concentrated to 1 mg/ml using a vivaspin 20 (GE Healthcare) and store at −80 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 ml column (GE Healthcare), equilibrated with buffer A (50□mM NaH2PO4 (pH 8) ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified over 1-2 ml His-Trap FF columns (GE Healthcare) with elution buffer containing 250 mM Imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... coli or insect cells as fusion proteins subcloned into pGEX-6P-1(GE Healthcare) or te pFastBac-1 vector (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 mL column (GE Healthcare), equilibrated with buffer A (50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Microbiology 2020Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with corresponding antibody and chemiluminescence was visualized according to the manufacturer’s instructions (ECL Advanced; GE Healthcare).