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Citations for GE Life Sciences :
201 - 250 of 6415 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Polyclonal antibody 2134 supernatent was purified using a Protein A affinity column (GE Healthcare). Serum was neutralized with 1/10 volume 1 M Tris pH 8 ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were immobilized on the Series S Sensor Chip Protein A chip (GE Healthcare), and then SARS-CoV-2-RBD-his protein with a gradient concentration from 1.25 nM to 40 nM was injected into the chip ...
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bioRxiv - Immunology 2020Quote: ... IgG antibodies were purified from rabbit serum using protein A sepharose beads (GE Healthcare), and these IgG antibodies were administered to brain ischemia model mice as a control antibody ...
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bioRxiv - Neuroscience 2022Quote: ... for mouse antibodies or Protein A Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for rabbit antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Protein bands reacting with antibodies were visualized using chemiluminescent detection reagent (GE Healthcare®) on an iBright FL1500 (ThermoFisher Scientific®).
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bioRxiv - Microbiology 2023Quote: ... 20 µg of anti-HA monoclonal antibodies crosslinked to protein G MicroBeads (GE Healthcare) were added to the cleared lysate and incubated overnight at 4 °C ...
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bioRxiv - Genetics 2023Quote: ... Chromatin fragments bound to antibody were captured on Protein A-Sepharose beads (GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...
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bioRxiv - Immunology 2019Quote: Binding to FcγRs was carried out using a Biacore T200 biosensor (GE Healthcare). Recombinantly expressed FcγRS (R&D systems or Sino Biologicals ...
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bioRxiv - Biochemistry 2020Quote: Measurements of heparin binding were performed in a Biacore T200 instrument (GE Healthcare) using a heparin-coated chip (Xantec) ...
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bioRxiv - Biochemistry 2020Quote: ... which allows affinity purification by binding to a StrepTactin Sepharose resin (GE Healthcare). Cells were grown in LB medium at 37°C with agitation to OD600nm = 0.8 ...
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bioRxiv - Cell Biology 2020Quote: MCAK was first affinity purified by binding to His-Trap HP (GE Healthcare) in binding buffer (50mM NaPO4 ...
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bioRxiv - Molecular Biology 2021Quote: ITC binding assays were carried out on a MicroCal ITC200 calorimeter (GE Healthcare) at 25 °C in a buffer containing 20 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: All analytical SEC binding assays were conducted on an ÄKTAmicro system (GE Healthcare) using a Superose 6 Increase 3.2/300 column (Cytiva ...
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... HRP secondary antibodies (1:1000; GE Healthcare) were used depending on the primary antibody ...
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bioRxiv - Molecular Biology 2019Quote: ... or Ni-NTA-Agarose (GE healthcare, His-B-MYB). Lysates of HeLa cells expressing HA-tagged B-MYB constructs were incubated with 5 mg immobilized GST or GST-WW1/2-YAP for 3 hours at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... Sera-Mag Speed Beads A and B (Ge Healthcare) were vortexed until the pellet was dissolved ...
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bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Microbiology 2020Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-BrdU antibodies were conjugated with protein A-sepharose beads overnight at 4C (GE Healthcare). Then the RNAs were bound to the beads ...
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bioRxiv - Molecular Biology 2020Quote: ... The antibody was pre-incubated with 20 μl of prewashed protein G Sepharose (GE healthcare) at 4°C for 1 hr ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected using horseradish peroxidase-coupled secondary antibodies (GE Healthcare Life Sciences, Piscataway, NJ) and ECL Plus or ECL Prime western blotting detection reagents (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected using horseradish peroxidase-coupled secondary antibodies (GE Healthcare Life Sciences, Piscataway, NJ) and ECL Plus or ECL Prime western blotting detection reagents (GE Healthcare Life Sciences) ...
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bioRxiv - Bioengineering 2021Quote: ... The antibody secreted into the culture medium was purified using Protein A resin (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using anti-SERCA2 antibody and protein A-coupled Sepharose beads (GE Healthcare). Beads were washed twice with high salt buffer (50 mM Tris/Cl ...
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bioRxiv - Molecular Biology 2020Quote: ... the antibody was pre-incubated with 20 μl of prewashed protein G sepharose (GE healthcare) at 4°C for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... The mouse monoclonal antibodies were purified from hybridoma supernatant using protein G columns (GE Healthcare). The specificity of CAST monoclonal antibodies was confirmed by immunohistochemistry with CAST KO mice (56) ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins recognised by the antibodies were visualised using ECL prime (GE Healthcare, Chicago, IL, USA) and digital images were acquired with a LAS4000 mini imager (FUJIFILM Wako Pure Chemical ...
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bioRxiv - Microbiology 2021Quote: ... IgG1 antibodies were isolated from cell supernatant using a HiTrap protein A column (GE Healthcare) and Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva, GE Healthcare). Antibodies were dialyzed overnight in PBS and filter-sterilized though 0.22 µm Spin-X filters ...
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bioRxiv - Microbiology 2022Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Immunology 2023Quote: ... the recombinant monoclonal antibodies were purified from the supernatant with protein G beads (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The antigen–antibody complexes were pulled down by 30 μl Protein A-Sepharose (GE Healthcare) and specific proteins were detected by the Western blotting analysis ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified from clarified supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Free azide-PEG3-biotin was removed with MicroSpin G-25 Columns (GE Healthcare). The flow-through fraction was mixed with 300 µl of Dynabeads M280 streptavidin (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... Biotin excess was removed by SEC on a Superdex 200 column (GE Healthcare). In the SEC profile ...
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bioRxiv - Biophysics 2022Quote: ... excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... followed by the removal of biotin by desalting on PD10 columns (GE Healthcare). The desalted lysates were then applied to streptavidin agarose resin (100 µl ...
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bioRxiv - Immunology 2022Quote: ... excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare). The control MHC-I (HLA-A2 ...
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bioRxiv - Biochemistry 2020Quote: Binding studies were performed at 25°C using a MicroCal iTC200 instrument (GE Healthcare). ANT(9 ...
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bioRxiv - Biophysics 2020Quote: SPR binding experiments were collected on a Biacore T200 or S200 Instrument (GE Healthcare). Neutravidin (Pierce ...
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bioRxiv - Synthetic Biology 2020Quote: ... Aggregated protein (on 1 micron magnetic Sera-Mag beads; GE Healthcare) was then digested with proteomics-grade trypsin (1:75 w/w ...
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bioRxiv - Bioengineering 2020Quote: ... for HRP-conjugated antibodies: donkey monoclonal anti-mouse (1:10000) and donkey monoclonal anti-rabbit antibody (1:10000, GE Healthcare) diluted in 2% BSA-TBST ...
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bioRxiv - Cell Biology 2019Quote: ... anti-mouse secondary antibody (1:4000, GE Healthcare) or alpaca anti-chicken (1:4000 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Rabbit secondary antibody (GE healthcare,1:10000). Membranes were developed by the Odyssey Fc Imaging system (LI-COR Bioscience).
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bioRxiv - Plant Biology 2021Quote: ... Purification of the unprocessed form of cardosin B was performed by IMAC using a 1 mL HisTrap column (GE Healthcare, USA) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellet was dissolved in 10 mL of buffer B and loaded onto a 1 mL HiTrap Heparin HP sepharose column (GE Healthcare) equilibrated with buffer B supplemented with 0.1 M NaCl ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...