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Citations for GE Life Sciences :
501 - 550 of 6604 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: The binding affinity of b96.11 to apo- and holo- GAD65 was measured using surface plasmon resonance (BIAcore T-100, GE Healthcare). Purified monoclonal b96.11 IgG antibody was captured on a CM5 sensor chip with immobilised Protein G ...
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bioRxiv - Biochemistry 2023Quote: ... Batch binding was performed overnight with Glutathione resin (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat. no. 17-5132-02) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The protein bands were detected by incubating the membrane with primary antibodies (see below) overnight at +4°C and horse radish peroxidase-conjugated secondary antibodies (1:5000, NA934 or NA931, GE Healthcare) for 1 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare) for immunoblotting ...
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bioRxiv - Bioengineering 2020Quote: ... Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare) affinity columns.
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bioRxiv - Cell Biology 2021Quote: ... and proteins were collected on protein G-Sepharose beads (GE Healthcare) for an additional 4 h at 4°C with gentle shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... a mixture of protein A and protein G sepharose (GE Healthcare life sciences ...
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bioRxiv - Neuroscience 2021Quote: ... The reaction was quenched and free biotins were separated using a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: PBMCs from Donors A and B were isolated by Ficoll-Paque (GE Healthcare, Pittsburgh, PA) gradient centrifugation and cryopreserved ...
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bioRxiv - Molecular Biology 2021Quote: ... and the respective feed media ActiCHO Feed a and ActiCHO Feed B (both GE Healthcare). Inoculation cultures were obtained by expansion of ConM and ConS lead clones from MCBs in production medium (ActiCHO P medium supplemented with 8 mM L-glutamine ...
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bioRxiv - Plant Biology 2022Quote: Method B) Genomic DNA was extracted using the Nucleon Phytopure DNA extraction kit (GE Healthcare Gio-Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over Nap10 (GE Healthcare, 17-0854-01) and PD10 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over NAP- 10 (GE Healthcare, 17-0854-01) and PD-10 (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Analysis was done by western blotting using the primary antibodies directed against H3K36me3 (ab9050, 1:2000) or H3K36me2 (ab9049, 1:2500) and as secondary antibody the anti-rabbit HRP (Na934v, GE Healthcare, 1:5000). The signal was detected by chemiluminescence after the addition of Pierce™ ECL Western Blotting substrate.
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bioRxiv - Immunology 2022Quote: IgG was purified from cell culture media or plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Immunology 2022Quote: ... Human IgG and IgA antibodies were purified from donors’ sera by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) and peptide M-coupled agarose beads (Invivogen) ...
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bioRxiv - Immunology 2019Quote: Total IgG antibodies were captured from 2 µL of plasma using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Developmental Biology 2019Quote: ... Antibodies used for immunoblotting are described in Table S3 HRP-conjugated anti-rabbit secondary antibody was used (Table S3) and protein detection was performed with ECL Select Western Blotting Detection Reagent (GE Healthcare, RPN2235) on an ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... horseradish peroxidase-linked secondary antibody (donkey monoclonal, 1:2000, GE Healthcare, Bucks, UK) for 45 min ...
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bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated anti-rabbit antibody (sheep polyclonal, used at 1:3000, GE Healthcare) and HRP-conjugated anti-chicken antibody (donkey polyclonal ...
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bioRxiv - Microbiology 2019Quote: ... Horseradish peroxidase-conjugated secondary antibodies (1:5000) and enhanced chemiluminescence reagents (GE Healthcare) were used to detect the bands on a Bio-Rad ChemiDoc MP system.
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bioRxiv - Cancer Biology 2020Quote: ... Horseradish peroxidase-conjugated α-mouse antibody (1:10,000; NA931V, GE Healthcare, IL, USA) was used as a secondary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... and HRP-conjugated anti-rabbit antibody (NA934V, GE Healthcare Life Sciences, 1:10,000). Antigen/antibody complexes were visualized by Enhanced Chemiluminescence (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... or HRP-conjugated anti-rabbit antibody (NA934V, GE Healthcare Life Sciences, 1:10,000) for one hour at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... and goat anti-rabbit IgG-horseradish peroxidase secondary antibody (1:5,000, GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies anti-IgG (anti-rabbit or anti-mouse, GE Healthcare, 1:5,000) conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2020Quote: ... After incubation with 1:10,000 anti-mouse horseradish peroxidase conjugated antibody (GE Healthcare), proteins were detected as described above.
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies were donkey anti-rabbit horseradish peroxidase (GE Healthcare NA934, 1:2,000) and goat anti-rat horseradish peroxidase (GE Healthcare NA935 ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibodies were mouse IgG HRP-linked (NA931, GE Healthcare; 1:5000) or rabbit IgG HRP-linked (NA934 ...
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bioRxiv - Microbiology 2022Quote: ... followed by HRP-linked secondary antibodies (1:5,000) and enhanced chemiluminescence (GE healthcare). α-His antibody (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... a fluorescent Cy5-conjugated goat anti rabbit secondary antibody (GE Healthcare; 1:5,000) was used and the fluorescent signal acquired with a Typhoon fluorescent scanner (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... along with corresponding species IgG secondary antibodies (1:5000, GE Healthcare, Fisher Scientific) were utilised ...
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bioRxiv - Cancer Biology 2021Quote: ... and visualized using HRP-conjugated secondary antibodies (donkey anti-rabbit, 1:15,000, and sheep anti-mouse, 1:15,000, GE Healthcare) and ECL substrate (SuperSignal West Femto Maximum Sensitivity Substrate and Atto Ultimate Sensitivity Substrate ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 1 h at room temperature with HRP-conjugated rabbit or mouse secondary antibodies (1:50,000, GE Healthcare). Enhanced chemiluminescence (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The sonicated proteins were pre-cleared with a 25μl protein A sepharose and protein G sepharose beads mixture (GE Healthcare) and the appropriate antibodies were added to the cleared extract and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin was precleared for 1 hour at 4 °C with blocked protein A Sepharose beads (GE Healthcare, Eindhoven ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... The plexin protein in the supernatant was captured using a 1 mL HisTrap FF column (GE Healthcare) and eluted by Buffer B containing 10 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was loaded onto a 1 ml HiTrap Heparin HP column (GE Healthcare, 17-0406-01) and eluted with a linear gradient from buffer A (20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: The heterologous proteins containing His-tags were purified via 1 ml Ni-NTA HisTrap columns (GE Healthcare). The cells were lysed in 50 ml lysis buffer containing 50 mM Na-phosphate buffer pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... 0.03 % Triton-X 100) and proteins were eluted directly into a 1 mL HisTrap column (GE Healthcare) using purification buffer supplemented with Desthiobiotin 2.5 mM (IBA Solutions For Life Science™) ...
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bioRxiv - Biochemistry 2022Quote: ... including a reference channel for online background subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface (Biacore T100 Control Software; GE Healthcare). All measurements were performed with 2-fold serial dilutions using 60 s association followed by a dissociation time of more than 240 s at 25 °C with a flow rate of at 30 μl min−1 ...
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bioRxiv - Immunology 2021Quote: ... PPACK-thrombin or fibrinogen in acetate buffer pH4.5 was immobilized to a chip surface (1000 or 10000 RU) using NHS/EDC-amine coupling and residual binding sites blocked by 1M ethanolamine (GE Healthcare). FH (0.5-62nM ...
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bioRxiv - Microbiology 2021Quote: ... assay was performed to determine the binding affinities of aCPSF1 to poly-U-tract containing RNA on a BIAcore 8K instrument (GE Healthcare) as described previously (Zhang et al. ...
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bioRxiv - Biophysics 2022Quote: Binding kinetics of ACE2 and SARS-CoV-2 RBDs were determined by surface plasmon resonance using a Biacore S200 (GE Healthcare). All experiments were performed in a running buffer composed of 10 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then diluted 10 times with mono Q binding buffer (50 mM HEPES, pH 8.0) and applied to a MonoQ 5/50 GL column (GE Life Sciences) connected to an AKTA Pure system (GE Life Sciences). ...
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bioRxiv - Biochemistry 2020Quote: ... Fluorescence was detected where binding occurred between SKI and PSMAD3–SMAD4 complex and measured with a Typhoon FLA 9500 biomolecular imager (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... assay was performed to quantify the binding avidity of the recombinant mos-tri-RBD to the receptor hACE2 using BIAcore 8K (GE Healthcare) with NTA chips ...