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Citations for GE Life Sciences :
3301 - 3350 of 4276 citations for Recombinant Human Pro NGF Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... we performed size exclusion chromatography on the concentrated proteins in a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an Akta Ettan fast protein liquid chromatography (FPLC ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were separated using standard SDS-PAGE electrophoresis techniques and transferred onto 0.45 μm nitrocellulose membranes (GE Healthcare, Amersham, U.K.) where non-specific binding was blocked by 5% (w:v ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by size exclusion chromatography on HiLoad 26/600 Superdex 200 prep grade column (GE Healthcare) equilibrated with the working buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Microbiology 2022Quote: ... and passed through a 0.45-μm filter prior to protein purification via affinity chromatography using an AKTA Start FPLC system (GE Healthcare). The lysate was loaded into a HisTrap HP 5 mL column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... TEV protease was added during dialysis to remove the N-terminal His6-tag.The protein was retrieved from dialysis and loaded onto a 1ml HiTrap SP HP IEX column (GE Healthcare) and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biophysics 2022Quote: ... Protein eluted from the affinity columns was further purified by size-exclusion chromatography using a Superdex 75 column (GE healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2022Quote: ... Rac1 protein went through a nickel affinity column followed by a Resource Q column and finally Superdex 75 (GE Healthcare) before concentration to 25mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Antibody supernatants were harvested seven days following transfection and purified using protein G affinity chromatography following the manufacturer’s protocol (GE healthcare). In order to validate the functional relevance of Arg100 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins (500 µl) were loaded on Superdex 75 10/300 GL or Superdex 200 10/300 GL columns (GE Healthcare) and eluted with buffer 20 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... CM were collected after 48h of transfection and filtered through 0.45 μm syringe filters and used for protein purification via ÄKTA pure 25 FPLC (GE Healthcare) equipped with 1-ml HisTrap affinity column (HisTrap HP ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare) in elution buffer (40 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... After gentle stirring for one hour aggregates were removed by centrifugation at 75,600g and the solubilised membrane proteins were applied to a StrepTrap column (GE healthcare) which was then washed in TBS supplemented with 0.01% LMNG ...
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bioRxiv - Immunology 2020Quote: ... IgGs were purified from ∼1-3 ml of serum using equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted off the resin with 0.1 M glycine pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Neuroscience 2020Quote: ... labelled protein samples were subjected to size exclusion chromatography at 4°C (Superdex 200 Increase 10/300 GL, GE Healthcare) in 50 mM phosphate (pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cooled down on ice and ultracentrifugated at 160,000xg for 1hr at 4°C and loaded onto a 5 mL Protein A (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... protein samples were concentrated and further purified by preparative SEC using a HiLoad 26/600 Superdex 200 pg (GE Healthcare) column ...
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bioRxiv - Immunology 2021Quote: ... the protein bands on SDS-PAGE gels were scanned and quantified by laser densitometry (Personal Densitometer SI; GE Healthcare, IL). The densities of the Hoc ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Resuspended protein was filtered using a 0.1 um pore size centrifugal filter and loaded onto a Sephadex S200 column (GE Healthcare) for size exclusion chromatography (SEC) ...
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OxoScan-MS: Oxonium ion scanning mass spectrometry facilitates plasma glycoproteomics in large scalebioRxiv - Systems Biology 2022Quote: ... IgG was isolated from 5 μl of serum using 30 μl of Protein A Sepharose (GE Healthcare, Eindhoven, The Netherlands). Sample mixtures were incubated under agitation at 650 rpm for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Polyclonal IgG from subject 391370 was isolated from heat-inactivated plasma using Protein G Sepharose 4 Fast Flow (GE Healthcare). Purified IgG was buffer exchanged into Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Cell Biology 2022Quote: ... Bio-Rad (4–15%) gradient gels were used for gel electrophoresis Proteins were blotted onto 0.2 µm nitrocellulose membranes (Amersham Protran, GE Healthcare) using the Thermo Scientific Pierce G2 Fast Blotter ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a Superdex 200 10/300 GL column using an ÄKTA chromatography system (GE Healthcare Life Sciences). 0.5 ml or 1 ml fractions were collected and analyzed by Western blotting using antibodies against HOIP and V5.
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein solution is again filtered before being loaded onto an equilibrated Superdex 200 size exclusion column (GE Healthcare) to separate the FMRP constructs from the Ulp protease and the His-SUMO fusion tag ...
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bioRxiv - Physiology 2022Quote: ... Proteins were visualized by chemiluminescence (#RPN2232 ECL and #RPN2235 ECL select Western blotting detection reagent – GE Healthcare, Little Chalfont, U.K.) and quantified using ImageLab software v ...
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bioRxiv - Developmental Biology 2022Quote: ... About 500-1,000 resonance units of each of the biotinylated proteins were immobilized on a SA sensor chip (GE Healthcare), using a Biacore S200 machine (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... the protein sample was concentrated and exchanged with phosphate buffer saline (PBS, pH 7.5) using a PD10 column (GE Healthcare) and stored at -80°C ...
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bioRxiv - Biochemistry 2020Quote: The purified rhGFAT2-his protein was subjected to a size exclusion chromatography using a Superdex 200 column (GE Healthcare, USA). The column was equilibrated with 1 CV of 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Elution was performed with the lysis buffer containing 250 mM imidazol pH 8.0 and eluted proteins were directly attached on a 10 ml Streptavidin Sepharose (GE Healthcare). Proteins were extensively washed with Strep buffer (20 mM Hepes ...
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bioRxiv - Cell Biology 2019Quote: A375 nuclear extracts and FLAG-USP7-containing protein complexes were applied to a Superpose 6 size exclusion column (GE Healthcare) that had been equilibrated with dithiothreitol-containing buffer and calibrated with protein standards (Amersham Biosciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted protein was dialyzed against storage buffer and applied to a HiTrap Q HP anion exchange column (GE Life sciences) pre-equilibrated in buffer A (50 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... and the eluted protein was further purified by size-exclusion chromatography on HiLoad Superdex 200 16/60 column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Genetics 2019Quote: ... Lysate was clarified by centrifugation at 25,000xg and soluble protein was purified using Co2+ affinity chromatography (TALON column, GE Healthcare) followed by anion-exchange chromatography (Q-FF ...
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bioRxiv - Cell Biology 2019Quote: ... The protein was purified via a two-step protocol of Ni-affinity using a 1ml His-Trap column (GE Healthcare) in Ni-affinity buffer [50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... the dialyzed protein was filtered and loaded on a hydrophobic HiTrap Butyl HP 1 ml column (GE healthcare life sciences), pre-equilibrated with 5 column volumes of buffer AButyl (500 mM ammonium sulfate ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins into rehydration buffer were incubated with an 18 cm immobilized pH 3–11 nonlinear gradient dry strip (GE Healthcare) for 16 hours at 20°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Eluted proteins were further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Life Sciences). Purified proteins were concentrated by ultrafiltration prior to flash freezing with liquid nitrogen.
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bioRxiv - Cell Biology 2019Quote: ... and for additional 4 hours with 40 µl of pre-washed Protein G Sepharose 4 Fast Flow beads (GE Healthcare) in a rotating wheel ...
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bioRxiv - Molecular Biology 2019Quote: ... and then the eluate was incubated overnight on a rotator at 4°C.The protein was resolved on a HiLoad 16/600 Superdex 200 Column (GE Healthcare) pre-equilibrated with storage buffer (20 mM HEPES-KOH ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was incubated with either Glutathione Sepharose® 4B (for GST-tagged proteins, GE Healthcare, #17-0756-01) or Protino Ni-TED resin (for SUMO-tagged proteins ...
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bioRxiv - Microbiology 2020Quote: ... all Fabs were expressed in ExpiCHO cells and purified using a HiTrap Protein G HP column (GE Healthcare, Chicago, IL) followed by size exclusion chromatography as used for the IgG1 but in Tris Buffered Saline (TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... MnCl2 or CaCl2was titrated into a sample cell containing 0.1 mM catalytic domain of the g12777 protein using an iTC200 calorimeter (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... the proteins were first purified from bacterial cell lysates using His-Trap HP (Ni-NTA) columns (GE Healthcare Life Sciences), as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein then was purified by ion-exchange chromatography through a HiPrep FF Q-column (20 mL, GE Healthcare; FPLC). After the column was equilibrated and washed with Buffer C ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation for 1 hour at room temperature proteins were purified using PD MiniTrap™ G-25 columns (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: Analytical size exclusion chromatography was performed with 20μl protein samples loaded on a Superdex 200 Increase 10/300 GL column (GE Healthcare) mounted on an Agilent 1260 HPLC ...
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bioRxiv - Physiology 2020Quote: ... YFP-tagged proteins were visualized by scanning the wet PAGE gels with a fluorescence scanner (Typhoon, GE Healthcare, München, Germany). Individual bands were quantified with ImageJ (53).
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bioRxiv - Biochemistry 2020Quote: ... The proteins were concentrated ,applied to gel-filtration using a Superdex 75 prep grade HiLoad 16/60 column (GE Healthcare) equilibrated in buffer K (10 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...