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Citations for GE Life Sciences :
3501 - 3550 of 4276 citations for Recombinant Human Pro NGF Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Then the proteins were concentrated with Amicon Ultra 10K filter (viMillipore) and applied to HiLoad Superdex 200 16/60 column (GE Healthcare). Fractions containing either AsCas12a or SpCas9 were collected ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the heparin-purified protein was concentrated by ultrafiltration and applied to a size exclusion column (Superdex 200 16/600 column, GE Healthcare). The protein was eluted with Buffer C (20 mM HEPES ...
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bioRxiv - Plant Biology 2019Quote: ... The N-terminal 6xHis tag was cleaved with TEV protease and the protein further purified using a heparin (LUXMYB) or Superdex 200 (LUXFL) column (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... analytical gel size-exclusion chromatography was performed with 1-2 mg protein in buffer A on a Superdex 75 10/300 column (GE Healthcare), to which a static light-scattering (SLS ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing EftMHM4 or EftMPAO1 were pooled and the protein further purified by gel filtration chromatography on a Superdex200 10/300 column (GE Healthcare) equilibrated with gel filtration buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... Labeled proteins were separated from the free fluorophore using a NAP-5 Sephadex G-25 desalting columns in labeling buffer (GE Healthcare). The labeling efficiency was determined using an extinction coefficient of 5400 M-1cm-1 at 335 nm for IAEDANS and the protein concentration was determined by Bradford assay using BSA as a standard ...
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bioRxiv - Biochemistry 2019Quote: ... no spectral changes were observed for WT protein anymore and the sample was applied to a size exclusion column (HiLoad 10/300 Superdex 75 pg column, GE Healthcare) and the dimeric fraction of about 50% of the total protein was pooled ...
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bioRxiv - Biophysics 2020Quote: ... The protein samples were centrifuged at 12,000g for 10 min to remove any protein precipitation and then loaded into a gel filtration column (Superdex 200 Increase 10/300GL, GE Healthcare) coupled with an 18 angle light scattering detector (DAWN HELEOS II ...
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bioRxiv - Plant Biology 2019Quote: ... Purification tags were removed by incubation with 3C protease (10 μg/mg fusion protein) followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/60 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... Rab8-His6 proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Unspecifically bound proteins were removed by a step wash with 5% buffer B (50 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... The remaining lysate was then incubated overnight at 4°C with anti-GFP (Rudner et al., 1999) or anti-SMC (Lindow et al., 2002) antibodies and subsequently incubated by Protein A-Sepharose resin (GE HealthCare) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Supernatant was filtered and N-glycosylated proteins isolated by affinity chromatography using Concavaline A columns (HiTrap Con A 4B, GE Healthcare) in AKTA FPLC (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... The column was calibrated with protein standards of known molecular weight and stokes’ radius (Low and High Molecular Weight Kit, GE Healthcare) according to manufacturer’s protocol.
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bioRxiv - Biochemistry 2019Quote: ... of total cellular protein was separated by SDS-PAGE using 4-12.5 % Bis-Tris gels and transferred to nitrocellulose membrane (GE Healthcare # 10600006). SeeBlue pre-stained protein standard (Invitrogen # LC5925 ...
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bioRxiv - Immunology 2019Quote: ... All affinity-purified proteins were size-exclusion-purified using a Superdex 200 Increase 10/300 GL column (GE Healthcare, catalog 28990944) in HEPES buffered saline (50 mM HEPES-NaOH ...
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bioRxiv - Physiology 2019Quote: We purified individual isoHbs by means of anion-exchange fast-protein liquid chromatography (FPLC) using an Äkta Pure system (GE Healthcare). Stripped hemolysate was loaded on the prepacked HiTrap Q-HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the desired protein were pooled and gel filtration was performed on Superdex 75 or 200 10/300 GL columns (GE Healthcare) mounted into an ÄKTA P100 chromatography system (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... One column contained total E.coli proteins covalently coupled to cyanogen bromide beads and the second column contained purified GST protein coupled to cyanogen bromide beads (GE Healthcare). Serum was cleaned by sequential incubations of 3 – 5 hours at room temperature with each column after which it was analyzed on an SDS page gel for lack of cross reactivity with GST and E.coli proteins from a total cell extract ...
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bioRxiv - Bioengineering 2019Quote: Product quality assays were performed on samples purified by Protein A chromatography using MabSelect SuRe LX resin (GE Healthcare, Amersham, UK). The column was equilibrated in a citrate/sodium chloride buffer at pH 7 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was further purified using reverse affinity chromatography on Ni-Sepharose followed by gel filtration (Superdex 200 16/60, GE Healthcare). Protein in gel filtration buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated protein was further purified using size-exclusion chromatography with a Superdex 75 10/300 GL column (GE Life Sciences) equilibrated in Buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... A 50-μl protein sample (1-2 mg/ml) was analyzed on a Superdex S-200 10/300 GL column (GE Healthcare) pre-equilibrated with a buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2019Quote: ... The solubility tag was cleaved with TEV protease and the protein further purified by size exclusion chromatography (Superdex75 GL300/10/300; GE Healthcare) at 4°C in a SEC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were digested with PreScission protease to remove tags and further purified by superose-6 increase column (GE Healthcare) in TBS buffer with 0.1% digitonin or 40 μM GDN ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant containing the soluble protein fraction was loaded onto a diethylaminoethyl ion-exchange column (DEAE-Sepharose Fast-Flow, GE Healthcare), followed by a Nickel-ion affinity column (Chelating-Sepharose Fast-Flow ...
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bioRxiv - Biochemistry 2019Quote: ... All constructs used for protein expression and purification in this study were GST fusions expressed from pGEX-4T1 (GE Life Sciences). The native proteins without signal peptides were expressed in BL21(DE3 ...
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bioRxiv - Biochemistry 2019Quote: ... The pooled protein was further purified by size-exclusion chromatography on a Superose 6 10/300 column (GE Healthcare Life Sciences). MPEG-1 fractions were monitored using SDS-PAGE ...
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bioRxiv - Microbiology 2019Quote: ... and the protein was further purified by size exclusion chromatography using a Superdex 75 16 600 column (GE Healthcare, Freiburg, Germany) and a 20 mM Tris-HCl pH 7.5 buffer.
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bioRxiv - Molecular Biology 2019Quote: ... The protein was then concentrated and subjected to size-exclusion chromatography on a Superose 6 5/150 column (GE Healthcare, Germany) in plunging buffer (20 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... the labeled protein (M1-Alexa488) was separated from the free dye using size-exclusion chromatography (PD-10, GE Healthcare, Munich, Germany). The labeled protein was eluted with diluted PBS (pH 7.4) ...
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bioRxiv - Immunology 2019Quote: The binding and kinetic rates measurement of gp120 proteins against RV305 mAbs were obtained by surface plasmon resonance (SPR) using the Biacore 3000 instrument (GE Healthcare). SPR measurements were performed using a CM5 sensor chip with anti-human IgG Fc antibody directly immobilized to a level of 9000-11000RU (response unit) ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were isolated from the medium exploiting the affinity of the 6xHis tag for the HisTrap Excel (GE Healthcare, USA) affinity column ...
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bioRxiv - Biochemistry 2019Quote: ... and cleaved protein was concentrated and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated with 20 mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was eluted with IMAC buffer containing 330 mM imidazole and loaded onto a HiTrap SP HP column (GE Healthcare). The column was washed with ion exchange buffer (IEC ...
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bioRxiv - Microbiology 2019Quote: Samples were electrophoresed on a 15% SDS polyacrylamide gel and proteins were then blotted onto a nitrocellulose membrane (GE Healthcare, Amersham). Nitrocellulose was blocked with 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Physiology 2019Quote: ... which was purified via sequential protein-A affinity and size exclusion chromatography (SEC) techniques using MabSelect SuRe and Superdex 200 purification media respectively (GE Healthcare), as described elsewhere (Suriben et al ...
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bioRxiv - Physiology 2019Quote: ... and purified from conditioned media by sequential protein-A affinity and size-exclusion chromatographic (SEC) methods using MabSelect SuRe and Superdex 200 purification media respectively (GE Healthcare). All purified antibody material was verified endotoxin-free and formulated in PBS for in vitro and in vivo studies ...
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bioRxiv - Developmental Biology 2019Quote: Full-length dP75 protein with GST tag was purified from BL21 E.coli by using Glutathione Sepharose 4B GST-tagged protein purification resin (GE healthcare, 17075601) and following the protocol in GE healthcare webpage ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through that contained the untagged protein of interest was applied to an ion exchange chromatography HiTrap Q column (GE Healthcare). The unbound protein sample ...
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions containing target protein was subjected to buffer exchange with buffer A using a HiPrep 26/10 desalting column (GE Healthcare). Next ...
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bioRxiv - Genetics 2020Quote: ... The cap-enriched mRNA was prepared from 100 μg of total RNA with GST-eIF4EK119A protein and glutathione sepharose 4B (GE Healthcare), as described previously (Bilska et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... proteins were further purified by size exclusion chromatography (SEC) using the HiLoad Superdex 16/600 200 prep grade column (GE Healthcare) run in 20 mM Tris-HCl pH 8.0 and 150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... The GST and GST fusion protein extracts were mixed with glutathione Sepharose 4B beads (71024800-GE; GE Healthcare, Chicago, IL, USA) overnight at 4 °C and the mixtures were then washed three times with ice-cold PBS ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated to 2 mL and further purified by size-exclusion chromatography using HiLoad 16/600 Superdex 200 (Ge healthcare).
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bioRxiv - Biochemistry 2020Quote: ... BSA was used for calibration and proteins were separated on a Superdex 200 Increase 10/300 analytical SEC column (GE Healthcare) with a flow rate of 0.4 ml/min ...
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bioRxiv - Biochemistry 2020Quote: Elution peaks corresponding to a molar mass of a dodecameric or dimeric protein of PrgL32-208 were further analysed by SEC-MALS with the use of an ÄKTApure system (GE Healthcare) coupled to a miniDAWN TREOS II detector and an OptiLab T-rEX online refractive index detector (Wyatt Technology) ...
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bioRxiv - Biochemistry 2021Quote: ... or 100 to 300 µg samples of individual proteins were injected onto a Superdex 75 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM TrisHCl ...
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bioRxiv - Biophysics 2021Quote: ... Total protein was collected and incubated with anti-p75NTR (Alomone, ANT-007, 1ug) overnight at 4 °C and then incubated with Sepharose protein-G beads (GE Healthcare). Samples were then prepared for immunoblotting as described below.
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bioRxiv - Bioengineering 2020Quote: ... The antibody expression vectors were co-transfected into 293F cells and the cell culture supernatants containing secreted antibody IgG were purified by columns packed in-house containing Protein A Sepharose beads (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The target protein was eluted with 10 mM maltose and further purified via gel filtration on a Superdex S75 (GE Healthcare) column.