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Citations for GE Life Sciences :
3151 - 3200 of 4276 citations for Recombinant Human Pro NGF Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 300 µg of total protein extracts were purified using Streptavidin Sepharose High-Performance beads (GE Healthcare Bio-Sciences AB). For western blot analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare) for 2 hours at 4°C in a rotating wheel ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extracts were separated in SDS-PAGE gels and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Western blotting was performed according to standard protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... was used at 1:10,000 and proteins were visualised by enhanced chemiluminescence using the ECL-Plus system (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: Proteins were further purified by size-exclusion chromatography (SEC) using a Superdex 200 10/300 GL column (GE Healthcare) integrated in the NGC system ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant containing the cleaved protein was removed and applied to a size exclusion (SEC) column (HiLoad_16/60_Superdex75 prep- grade, GE Healthcare) equilibrated with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were then further purified by size-exclusion chromatography using a Superdex 75 300/10 column (GE Healthcare) with running buffer 20 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane (BTP) ...
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bioRxiv - Molecular Biology 2023Quote: The extract (supernatant after high-speed ultracentrifugation) was used for protein purification using the ÄKTA Xpress system (GE Healthcare), employing nickel affinity chromatography on the 5 mL column compatible with ÄKTA ...
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bioRxiv - Microbiology 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 20 mM Hepes pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 50 mM Hepes pH 7 ...
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bioRxiv - Immunology 2022Quote: ... the protein-containing supernatant was purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Healthcare), which consisted of a 1 mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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bioRxiv - Cell Biology 2023Quote: ... soluble proteins were applied to the size exclusion gel filtration column (Superdex 200 Increase 10/300 GL, GE Healthcare), using an Akta P-920 Pump FPLC system (Amersham ...
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bioRxiv - Cancer Biology 2023Quote: ... and IgGs were then purified from the culture supernatants by Protein A-column chromatography (GE Healthcare, 17-1279-01). We obtained 6 clones as follows ...
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bioRxiv - Biophysics 2023Quote: ... The untagged protein was purified using gel filtration chromatography (ÄKTA with Superdex-200 increase 10/300 column; GE Healthcare) and equilibrated with storage buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 200 μL of protein solution were loaded onto a Superdex 200 HR 10/30 gel filtration column (GE Healthcare) with a flow rate of 0.5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence microscopy data of intracellular GFP fusion proteins were collected using a DeltaVision microscope system (GE Healthcare Inc., USA) equipped with a pco.edge 4.2 sCMOS camera (PCO ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated with SDS-PAGE and subsequently transferred to Amersham Hybond-P PVDF Transfer Membrane (RPN303F, GE Healthcare). Membranes were blocked in 5% milk in Tris-buffered saline with 0.01% Tween 20 and incubated with the antibodies listed in Supplemental Table 4 ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed by centrifugation and proteins were purified from the conditioned media using rProtein A Sepharose (GE healthcare). The purified proteins were buffer-exchanged into a formulation buffer (20 mM His-HAc ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining purification steps were carried out at 4 °C using an ÄKTA protein purification system (GE Healthcare/Cytiva) for column chromatography steps ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies (anti-rabbit IgG, anti-mouse IgG or protein-A) coupled with horseradish peroxidase were from GE Healthcare, an anti-goat IgG secondary antibody was from Santa Cruz.
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bioRxiv - Biophysics 2023Quote: ... Proteins were concentrated by centrifugation in Vivaspin 20 ultrafiltration devices with a 100,000 molecular weight cut-off (GE Healthcare). Protein concentrations were quantified by UV spectroscopy with an extinction coefficient of 36380 M-1cm-1 (Drp1 ...
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bioRxiv - Biophysics 2023Quote: ... Protein was isolated from the resulting supernatant by affinity chromatography using Ni-Sepharose 6 Fast Flow beads (GE Healthcare), and eluted with a 100 mL linear gradient of column buffer with 500 mM imidazole ...
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bioRxiv - Immunology 2023Quote: ... Unpurified proteins contained in HEK293F filtered supernatants were captured on PGT145-functionalized CNBr-activated sepharose 4B beads (GE Healthcare) by overnight rolling incubation at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The precipitated proteins were purified based on nickel affinity purification by AKTA Prime Plus automated purification system (GE Healthcare). The sample passed over a 1 mL Nickel-Sepharose (HisTrap HP ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein in the supernatant was diluted to 1% Triton and incubate with Glutathione Sepharose beads (GE Healthcare) in a batch mode ...
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bioRxiv - Biochemistry 2023Quote: Histone proteins were mixed in stoichiometric amounts and eluted through a HiLoad 16/600 Superdex 200pg column (GE Healthcare) to produce H2A-H2B dimers and (H3-H4)2 tetramers separately 40 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were blotted for 80 min (350 mA, room temperature) onto Amersham Protran 0.2 μm nitrocellulose membranes (GE Healthcare). Membranes were blocked in 5% milk in TBS-Tween for at least 2 h and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Protein samples (4mg/mL; 80 µL) were separated on Superdex 200 Increase 10/300 GL Tricoedn column (GE Healthcare) in 20 mM Hepes containing 150 mM KCl ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were further purified by size-exclusion chromatography using a Superdex 200 column (Cytiva, formerly GE Life Sciences) in buffer B ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were further purified via size exclusion chromatography using a Superdex200 Increase 10/300 GL column (GE Healthcare) in running buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein bands were quantified by densitometry with ImageQuant TL software (version 7.0 GE Healthcare Life Sciences, Pittsburgh, PA, USA). PsbS abundance was quantified relative to WT based on a dilution series of WT PsbS protein.
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bioRxiv - Neuroscience 2023Quote: ... the protein solution was concentrated and further purified utilizing size exclusion chromatography with a SuperdexTM 75 column (GE Healthcare). The protein solution was concentrated to 9.2 mg/mL and used for crystallization.
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bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superose 6 Increase 10/300 GL column (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (1-5 mg/ml) were loaded individually on a Superdex 200 Increase 10/300 column (GE Healthcare), and the elution profiles were obtained using a tandem of sequentially connected UV-Vis Prostar 335 (Varian ...
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bioRxiv - Systems Biology 2024Quote: ... Proteins were resolved by SDS-PAGE and transferred onto nitrocellulose membrane (GE Healthcare, Amersham™ Protan™ 0.2µM NC). After detection of phospho-ERK1/2 by immunostaining with mouse anti-P-p44/42 MAPK XP (Cell Signalling ...
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bioRxiv - Systems Biology 2024Quote: ... Proteins were resolved by SDS-PAGE and transferred onto nitrocellulose membrane (GE Healthcare, Amersham™ Protan™ 0.2µM NC). ADCY bands were immunostained with mouse anti-ADCY5/6 (Santa Cruz ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing predominantly ATeam protein were pooled and applied to a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated with 20 mM tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... Protein TLR827–827-PA-His was purified with IgG beads (Cat#17-0969-01, GE Healthcare, Connecticut, CT, USA) and resuspended in 200 μl of NT2 buffer 66 and used for miRNA-protein co-IP ...
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bioRxiv - Biochemistry 2023Quote: ... The protein fractions were concentrated and finally subjected to Superdex 75 10/300 size exclusion chromatography column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... A875 cell extracts were incubated for 16 hr at 4°C on a rotating wheel with 0.8μg of anti-p75NTR antibody (ANT-007, Alomone) and then incubated with Sepharose protein-G beads (GE Healthcare). The beads were collected by brief centrifugation (2 min ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were further purified by gel filtration using a Superose 6 Increase 10/300 GL column (GE Healthcare). Snu71 fragments with residues 1-96 ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was further purified by size exclusion chromatography using a Superose 6 10/300 GL column (GE Healthcare) and eluted in 50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: Interactions between the DMC1 protein and the synthetic BRCA2 peptides were characterized using a VP-ITC Calorimeter (GE Healthcare). The peptides tested were wild type and F2406A variant of the PhePP (F2s6 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE using 4% or 5% polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with mouse monoclonal antibody generated against HA-tag peptide (YPYDVPDYA ...
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bioRxiv - Microbiology 2023Quote: ... The eluant of protein was concentrated and further purified using a Superdex-200 increase 10/300 GL (GE Healthcare) column equilibrated with a buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: The purified GST-tagged fusion protein WDR522-334 (100 μg) was bound to Glutathione Sepharose 4B (GE Healthcare, 28952360) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were resolved in 12% SDS-PAGE gels to detect TAMRA fluorescence using Typhoon FLA 9500 (GE Healthcare) and images were processed and formatted using ImageJ.