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Citations for GE Life Sciences :
3051 - 3100 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The protein was loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare, USA) and purified using Assay Buffer or Assay Buffer without EDTA (for use in DNA cleavage assays) ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was further purified using a Superdex Increase 10/300 GL column (GE Healthcare, Chicago, IL) equilibrated with buffer containing 350 mM NaBr ...
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bioRxiv - Cancer Biology 2021Quote: ... We subjected equal amounts of proteins to SDS-PAGE and transferred them to nitrocellulose membranes (GE Healthcare #10401197). Non-specific binding was blocked by incubation with TBST plus 5% non-fat milk ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was loaded on a HiPrep Q FF Anion Exchange Column (GE Healthcare Life Sciences, Marlborough, MA) and eluted by buffer with 300 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was loaded onto a His-Trap column using an ÄKTA protein purification system (GE Healthcare Life Sciences). The chromatography was run with a two-step gradient started with 100% starting buffer (500 mM sodium chloride ...
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bioRxiv - Biophysics 2021Quote: ... Cell pellets were sonicated and His-tagged proteins were purified using affinity chromatography (HisGraviTrap column from GE Healthcare).
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Conformational dynamics at microsecond timescale in the RNA-binding regions of dsRNA-binding domainsbioRxiv - Biophysics 2019Quote: SEC-MALS analysis of the purified proteins was performed on Superdex 75 10/300 GL column (GE Healthcare) using an Agilent HPLC system equipped with the 18-angle light scattering detector (Wyatt Dawn HELIOS II ...
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bioRxiv - Microbiology 2021Quote: ... The protein complex was then purified by SEC using a Superdex 200 Increase 10/300 GL (GE Healthcare) column ...
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bioRxiv - Cell Biology 2019Quote: ... for the detection of ubiquitinated protein aggregates or a 0.2 μm pore size cellulose acetate membrane (GE Healthcare) for the detection of huntingtin aggregates using a 96 well format Dot-Blot apparatus (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... All mAbs were purified from cell culture supernatant using a HiTrap protein G HP affinity column (GE Healthcare) and stored at −80 °C ...
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bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
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bioRxiv - Immunology 2020Quote: ... we used fritted deep well plates containing 25 μL of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Microbiology 2021Quote: Proteins resolved by SDS PAGE were transferred to a nitrocellulose transfer membrane (GE Healthcare Amersham™ Protran™) at 80 V for 75 minutes or 15 V overnight at 4°C using a Bio-Rad wet transfer apparatus ...
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bioRxiv - Immunology 2021Quote: ... The protein was further purified on a Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare). SDS-PAGE was performed to determine the purity and size of the protein ...
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bioRxiv - Cell Biology 2021Quote: Approximately 5 μg of GST or GST-tagged protein fragments were incubated in Glutathione-Sepharose beads (GE Healthcare) in GST-lysis buffer at 4°C overnight ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... The fractions that contained the protein were pooled and subjected to Hi-trap ion exchange chromatography (GE healthcare) for further purification.
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bioRxiv - Plant Biology 2020Quote: Thylakoid proteins were separated by SDS-PAGE and then transferred to a polyvinylidene membrane (Hybond P, GE Healthcare) using a tank blot system (Perfect Blue Web M ...
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bioRxiv - Cell Biology 2021Quote: ... protein extracts were subject to SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL). Primary antibodies used were anti-P2Y1 (Santa Cruz ...
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bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Salt concentration is adjusted and protein is loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES- KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... All target proteins were subjected to size exclusion chromatography using the HiLoad 16/600 Superdex 75 (GE Healthcare) equilibrated in 25 mM Tris pH8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... After protein fractionation by SDS-page samples were transferred to a nitrocellulose membrane (cat#10600002 from GE healthcare) and incubated with primary antibody solutions overnight at 4 °C (p21 ...
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bioRxiv - Immunology 2022Quote: The total IgG was purified from murine serum using Protein G Sepharose 4 Fast Flow columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein was loaded and separated in SDS-page gels and then transferred to PVDF membrane (GE Healthcare). For blocking ...
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bioRxiv - Plant Biology 2022Quote: ... Protein was concentrated using a spin concentrator (Amicon, 10K MWCO) and injected onto Superdex 200 column (GE Healthcare) equilibrated in 25 mM HEPES (pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... protein samples were run on 12-15% polyacrylamide gels and transferred to nitrocellulose Hybond™-C (GE Healthcare) membranes via Bio-Rad Trans-blot Turbo transfer machine ...
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bioRxiv - Microbiology 2022Quote: ... the protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare). The peak corresponding to the protein was concentrated to about 12 mg/ml and flash frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Molecular Biology 2022Quote: ... The protein solutions were further diluted with buffer B and eIF4A1 fractions subjected to a ResourceQ (GE Healthcare) anion exchange column ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were concentrated and applied onto a Superdex 75 HiLoad 26/60 size-exclusion column (GE Healthcare) pre-equilibrated with gel-filtration buffer (10 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then visualized by chemiluminescence reagent (Cyanagen(XLS3,0100),Thermo Fisher(34580)) using Amersham Imager 600 (GE Healthcare) and band densitometry was measured using FIJI software ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were visualized using the Amersham ECL Prime Western Blotting Detection System and Amersham Hyperfilm ECL (GE Healthcare). The following primary antibodies were used ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was then incubated with 30 µl 50% protein A-Sepharose slurry (GE Healthcare, 17-0780-01) for 1 hr at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The recombinant protein in the supernatant of the cell lysate was mixed with Glutathione Sepharose resin (GE Healthcare) and the mixture was washed extensively in 10mM HEPES pH 7.3 ...
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bioRxiv - Developmental Biology 2022Quote: ... Molecular weights were determined using pre-stained protein ladders (Thermo Fisher Scientific, catalog #26616; GE Healthcare, catalog #RPN800E). Protein abundance was detected on membranes using the SuperSignal West Femto enhanced chemiluminescence kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Immunoprecipitation was performed by incubating sheared chromatin with Protein G Sepharose™ 4 Fast Flow beads (GE Healthcare) and antibodies ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was diluted to a total protein concentration of 100 μg/ml in binding buffer (GE Healthcare). Following purification ...
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bioRxiv - Biophysics 2023Quote: ... the protein was subjected to size-exclusion chromatography using sephacryl S-100 HR 16/60 column (GE HealthCare). The final purified protein was concentrated to 1 mM using Amicon (3 kDa cutoff ...
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bioRxiv - Neuroscience 2024Quote: ... The immuno-complexes were then incubated with 30 μl protein G sepharose beads (GE healthcare, 17-0618-01), which were pre-equilibrated with Lysis Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 μl of a protein A/G sepharose mixture (GE Healthcare; #17-0780-01 and #17-0618-01), pre-equilibrated in ChIP dilution buffer was added to the lysates and incubation continued for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... fritted deep-well plates were used containing 25 μl of settled Protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins were separated by 8-10% SDS-PAGE and transferred to a Hybond ECL nitrocellulose membrane (GE Healthcare) or Amersham Protran 0.45 μm NC nitrocellulose membranes (Carl Roth ...
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bioRxiv - Evolutionary Biology 2024Quote: ... all proteins were further purified by gel filtration on a HiLoad 16/60 Superdex 75 column (GE Healthcare) in 40 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... and fermentation supernatants were purified by Protein-A affinity chromatography using MabSelect™ column (GE Healthcare, Princeton, NJ). The bispecific antibody was further polished by cation exchange chromatography using Capto MMC (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: Analytical size-exclusion chromatography was performed using an AKTA fast protein liquid chromatography (FPLC) system (Amersham GE Healthcare). The concentrated samples were loaded into a Superose12 HR 10/30 FPLC column (GE ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then incubated with washed and pre-blocked protein G Sepharose beads (50 µl, GE Healthcare) for 45 mins at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The protein constructs were further purified by size exclusion chromatography using an ÄKTA Explorer FPLC system (GE Healthcare) loaded with two Superdex75 200 mL columns ...
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bioRxiv - Microbiology 2022Quote: ... Elution fractions containing Strep-tagged proteins were loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer 1 ...
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bioRxiv - Biochemistry 2023Quote: The protein was further purified by size exclusion chromatography (SEC) (Superdex 200 Increase 10/300 GL, GE Healthcare) in 50 mM Tris pH 7.5 ...