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Citations for GE Life Sciences :
3001 - 3050 of 4276 citations for Recombinant Human Pro NGF Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Soluble protein was manually applied at room temperature to a 5 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
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bioRxiv - Biophysics 2021Quote: ... The protein was further purified by size exclusion chromatography using a Superose 6 column (10/300 GL, GE Healthcare) equilibrated with 200 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified in one step by IMAC followed by size exclusion chromatography using ÅKTAxpress system (GE Healthcare) as previously described with some modifications (28) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 40-80 µg protein was used for western blotting (AmershamTM HybondTM - ECL nitrocellulose membranes, GE Healthcare, Chicago, IL) with a wet-transfer system (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... This mixture was then added to 30 μl of pre-equilibrated Protein G sepharose beads (GE Healthcare Life Science) and incubated ON under continuous agitation ...
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bioRxiv - Biochemistry 2021Quote: GST-MLLE protein was applied to Series S sensor chip CM5 bound with anti-GST antibodies (GE Healthcare BR100223), in Biacore T100 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then further purified by size-exclusion chromatography on a 16/600 Superdex 200 column (GE Healthcare) equilibrated in either 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... after which protein-containing fractions were pooled and loaded onto pre-equilibrated Superdex 200 HiLoad 16/60 (GE Healthcare) for size exclusion chromatography and elution was performed with 25mM Phosphate buffer pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were separated from unreacted fluorophores by passing over a Superpose 6 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then further purified by anion exchange chromatography using a 5 ml HiTrap Q HP column (GE Healthcare) across a gradient of 100 mM to 1M NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatants of the His6-tagged proteins obtained were incubated with 500μL Ni Sepharose (GE Healthcare 17-5318-06) and then washed twice with buffer A ...
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bioRxiv - Plant Biology 2020Quote: ... The secreted protein was purified from the supernatant by Ni2+ affinity chromatography on a HisTrap Excel column (GE healthcare), equilibrated in 50 mM KPi pH 7.6 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were then washed five times for 5 min and proteins were detected with Amersham ECL (GE Healthcare). Images were taken using the iBright Imaging System (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Purified analyte proteins were separated by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) in HBS-EP just prior to use in SPR experiments to remove any protein aggregates that might influence kinetic measurements ...
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bioRxiv - Microbiology 2020Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4 °C in 50 mmol L−1 Hepes at pH 8 ...
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bioRxiv - Systems Biology 2020Quote: ... Secondary horseradish peroxidase-coupled antibodies (anti-rabbit HRP, anti-goat HRP, Protein A HRP) were purchased from GE Healthcare. Immunoblots were incubated with ECL or ECL advance substrate (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The protein samples were further purified by gel filtration on a Superdex S75 HR 10/30 column (GE Healthcare). Samples were then concentrated to ~500 μM using 10 kDa cut-off centrifugal filter units (Amicon Ultra ...
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bioRxiv - Immunology 2021Quote: ... IgGs were purified from ~1 ml of plasma with equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted from the resin with 0.1 M glycine at pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... The protein was finally purified by gel filtration using a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with binding buffer ...
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bioRxiv - Microbiology 2021Quote: ... as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Plant Biology 2021Quote: ... soluble proteins were purified by affinity chromatography using a HisTrap Ni-NTA column (ÄKTA pure FPLC system, GE Healthcare) equilibrated in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare). Finally ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were then separated using a 7.5% SDS-PAGE gel and transferred to a nitrocellulose membrane (GE healthcare Lifesciences ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Individual pooled target proteins were further purified by passage through a Hiload 16/60 Superdex 200 column (GE Healthcare) with buffer E (150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... The cleaved protein was further purified through SEC in a Superose 6 increase 10/300 GL column (GE Healthcare), in buffer containing 50 mM phosphate pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by size-exclusion chromatography through a HiLoad 26/60 Superdex 200 column (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
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bioRxiv - Biophysics 2020Quote: ... SEC-MALS was performed at room temperature by passing 500 μl protein (12μM) at 0.3 ml/min flow rate through a Superdex S200 SEC column (GE Healthcare) pre-equilibrated with 10mM potassium phosphate buffer supplemented with 150mM NaCl and 2mM β-mercaptoethanol (pH 8.0) ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions (eluted at ~ 1 M NaCl) were then purified using a HiLoad 16/60 Superdex 75 (GE Healthcare) column (SEC ...
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bioRxiv - Cell Biology 2021Quote: Tag-free syndapin I and III were generated by expressing both proteins in the pGEX-6P vector (GE Healthcare) and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (Amersham™ Protran™ Premium 0.45 μm NC, GE Healthcare Life sciences, 10600003). For Msb2p-HA and Hkr1p-HA blots ...
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bioRxiv - Cancer Biology 2020Quote: ... All protein lysates were run on NuPage 4–12% gradient gels (LifeTechnology) and transferred to PVDF membrane (GE Healthcare). Membranes were blocked in 5% milk in TBST and stained with primary antibody overnight at +4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by ion-exchange chromatography using the HiTrap Heparin HP column (GE Healthcare Life Sciences) and a Mono Q anion exchange column (16/10 ...
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bioRxiv - Biochemistry 2022Quote: ... The untagged protein was concentrated and further purified on a Superdex 200 16/600 gel filtration column (GE Healthcare) in 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The histidine-tagged cleaved proteins where then loaded onto a 26/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with buffer C ...
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bioRxiv - Biochemistry 2022Quote: ... Then the interest protein was purified by gel-filtration chromatography using a Superose 6 gel filtration column (GE Healthcare) pre-equilibrated with HBS buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... fractions containing pure protein sub-complexes were subjected to size-exclusion chromatography (Superose 6 increase 10/300, GE Healthcare) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were cleaved overnight with 3C protease followed by gel filtration on Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Proteins were then transferred onto a nitrocellulose membrane (Amersham™ Protran™ 0.2 µm NC, GE Healthcare Life science) using a standard blotting procedure (80V for 2h ...
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bioRxiv - Biochemistry 2022Quote: ... after which protein-containing fractions were pooled and loaded onto pre-equilibrated Superdex 200 Increase 10/300 (GE Healthcare) for size exclusion chromatography ...
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bioRxiv - Microbiology 2022Quote: ... Proteins released in the soluble form were purified using Glutathione Sepharose 4B according to the manufacturer’s instructions (GE Healthcare). On-column thrombin digestion was performed to release the protein without the GST tag ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... C (W6/32) and rotated with 30 μl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved by SDS-PAGE using 15% gels and transferred onto nitrocellulose membranes (Amersham Protran, GE Healthcare, USA). Immunoblots were processed by enhanced chemiluminescence (ECL ...
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bioRxiv - Biophysics 2022Quote: ... All the eluted proteins were loaded into Superdex 200 pg or Superdex 75 pg size exclusion chromatography (GE Healthcare) to remove aggregates and potential contaminants.
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bioRxiv - Immunology 2022Quote: ... 5% Glucose and the proteins purified by cation-exchange chromatography using a HiTrap SP High-Performance columns (GE Healthcare), followed by size exclusion chromatography using a HiLoad 16/600 Superdex 75pg column (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were further purified by size-exclusion chromatography using an S200 Increase or a Superose S6 column (GE Healthcare), depending on protein size ...
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bioRxiv - Biochemistry 2020Quote: ... protein containing eluate was concentrated and applied to a HiLoad™ 16/600 Superdex™ 75pg column (GE Healthcare) in 20 mM Hepes pH 7.5 ...