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Citations for GE Life Sciences :
2951 - 3000 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Two mg of proteins in buffer were mixed with 50ul 7-Methyl-GTP-Sepharose 4B (GE Healthcare) previously equilibrated with extraction buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The cleaved proteins were separated by a 16/600 Superdex75 gel filtration column (GE Healthcare, Chicago, lL), followed by depleting the peak of lgMPZ peak or residual MBP by by applying to amylose resin ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were pooled and concentrated before separation on a Superdex 200 16/60 column (GE Healthcare) equilibrated in GF2 Buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then purified using a ResourceTM Q 6ml column (17-1179-01, GE Healthcare, Uppsala, Sweden) and a Superdex 200 Increase 10/300 column (28-9909-44 ...
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bioRxiv - Genetics 2023Quote: ... Protein containing fractions were further purified by size exclusion chromatography using Superdex 200 10/300 (GE Healthcare) gel-filtration column in SEC buffer (20 mM HEPEs pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were concentrated and gel-filtrated on a SuperdexTM 75 size exclusion chromatography column (GE Healthcare) equilibrated with stock buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples with indicated concentrations were loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... The cleaved protein was applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare, USA) pre-equilibrated with gel filtration buffer (150 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... The filtered supernatant included NMnPP protein was applied to a nickel-nitrilotriacetic acid (Ni-NTA, GE Healthcare) affinity column pre-equilibrated with buffer A ...
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bioRxiv - Immunology 2023Quote: ... The secreted antibodies in the supernatant were purified by protein G Sepharose 4 Fast Flow (GE Healthcare). Purified antibodies were dialyzed in PBS and sterile filtered (0.22 μm) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS/PAGE gel and transferred to Protran nitrocellulose membrane (0.2 μm, GE Healthcare). Immunoblotting was performed by incubating with primary antibodies diluted in Tris-buffered saline (20mM Tris pH 7.6 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare) in NETN buffer (20mM Tris PH8 ...
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bioRxiv - Plant Biology 2024Quote: ... 25 mg of protein was incubated with 25 µl IgG-sepharose 6 FAST Flow beads (GE Healthcare) for 1 h on a rotator at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted protein was separated on a 5 ml HiTrap STREP column (GE Healthcare, Little Chalfont, UK), and eluted with 2 mM desthiobiotin ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the protein was purified using Ni-NTA affinity columns on an ÄKTA pure system (GE healthcare), followed by size exclusion chromatography in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted proteins were loaded onto a HiTrap Q HP 5 ml anion exchange column (GE Healthcare) pre-equilibrated with QA buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The one-step purified protein was then loaded onto a HiTrap Heparin Sepharose HP column (GE Healthcare) and eluted from it in a salt gradient of 100-1000 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... proteins were separated according to molecular weight by SDS-PAGE using the Ettan DaltTwelve system (GE Healthcare). Radioactive gels were dried on Whatman paper and exposed to storage phosphor screens (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... All proteins were further purified by SEC using a Superdex 200 10/300 GL column (GE Healthcare) with 20 mM Tis-HCl at pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was additionally purified by size-exclusion chromatography (Superdex 200 16/60 column; GE Healthcare™).
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bioRxiv - Microbiology 2024Quote: ... Collected supernatant containing the Fc fused nanobody was incubated overnight with protein-A Sepharose beads (GE Healthcare), after which proteins were purified using poly-prep chromatography columns (Bio-Rad) ...
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bioRxiv - Biophysics 2024Quote: ... The cleaved proteins were further purified by cation exchange chromatography using HiTrap SP HP column (GE Healthcare) equilibrated in buffer C ...
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bioRxiv - Microbiology 2024Quote: ... The mAbs were isolated from the culture supernatants by affinity chromatography using Protein G beads (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Immune complexes were incubated with Protein G Sepharose® 4 Fast Flow (GE Healthcare, #17-0618-01) overnight at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Dialysed protein was further purified by ion-exchange chromatography using a HiTrap-FF Q column (GE Healthcare), resulting in the elution of N-myc proteins in the flow-through and at low salt concentrations ...
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bioRxiv - Plant Biology 2024Quote: ... 100 μg of APOs200 proteins were resolved in a Superdex Increase 200 10/300 GL (GE Healthcare) on an AKTA FPLC system (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were finally purified by passing through the HiLoad 16/600 Superdex 75 column (GE Healthcare). The nsp16/nsp10 complex was eluted as a single homogenous species in a final buffer containing 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The purified proteins were taken for the size exclusion chromatography column (Superdex 75G 16/600, GE Healthcare) using the AKTA Prime purification system (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified and buffer exchanged using the Superdex 300 Increase 10/300 GL (GE Healthcare) size exclusion column into the lysis buffer for storage and characterization ...
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bioRxiv - Biochemistry 2024Quote: ... Protein purification was conducted on a nickel affinity column (HisTrap HP, 1mL, GE Healthcare, 17-5247-01) using an ÄKTA Pure 25M Chromatography System (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). The membranes were then probed with the following antibodies ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated by SDS-PAGE and transferred to a Protran nitrocellulose membrane (GE Healthcare Life Sciences) using a wet transfer apparatus (Amersham Bioscience) ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing the target protein were pooled and immediately loaded onto a size-exclusion column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins from these gels were transferred onto a previously activated PVDF membrane (Hybond-P, GE Healthcare Ltd) in an electro-blotting apparatus (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... and then add peroxidase substrate solution to detect expression of the protein of interest (GE Healthcare Amersham).
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bioRxiv - Microbiology 2023Quote: HeLa cells grown to 90% confluency were transfected with ON-TARGETplus human Skp1 SMARTpool siRNA or non-targeting siRNA (GE Healthcare Dharmacon, Inc., Lafayette, CO) at a final concentration appropriate to the vessel size ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were further purified by gel filtration on a Hiload 16/600 Superdex 75 column (GE Healthcare) in the size exclusion buffer (50 mM Tris pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples were further purified by gel filtration chromatography on an ÄKTA Pure 25 FPLC (GE Healthcare) using a Superdex75 or Superdex75 increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µl of a Protein A sepharose (nProtein A Sepharose 4 fast flow, GE Healthcare, 17-5280-01) suspension was added and incubated for 1 hour at 4°C on a rotator ...
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bioRxiv - Biochemistry 2020Quote: ... The TEV-treated proteins were directly applied to a Superdex 200 column (16/60 prep grade, GE Healthcare) equilibrated with gel filtration buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were then purified by cation exchange using a 5-mL HiTrap SP HP column (GE Healthcare) and the collected fractions analyzed by SDS-PAGE ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using an AKTA FPLC with 1 mL nickel-charged HisTrap columns (GE Healthcare Life Sciences) for affinity chromatography of the His-tagged proteins ...
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bioRxiv - Molecular Biology 2020Quote: ... 200-300 µg of the indicated proteins were injected into a Superdex 200 10/300 GL (GE Healthcare) equilibrated at a flow rate of 0.3 ml per minute in 50 mM Hepes pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were isolated from the purified GST fusion proteins by cleaving with PreScission protease (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were further purified via gel filtration chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) prior to usage ...
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bioRxiv - Cell Biology 2020Quote: Sheared chromatin was pre-cleared with 50 µl protein A Sepharose 4 Fast Flow beads (GE Healthcare, Germany) per 500 µg chromatin for 2h ...
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bioRxiv - Cell Biology 2020Quote: ... The cleaved dCas9 protein was further purified with a 5 ml SP Sepharose HiTrap column (GE Life Sciences) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Immunology 2021Quote: ... The protein was further purified by size exclusion chromatography using Superdex 200 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES at pH 7.4 ...
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bioRxiv - Genomics 2020Quote: ... The signal of resolved protein was visualized by Amersham enhanced chemiluminescence (ECL) Western Blotting Analysis System (GE Healthcare) using Amersham Hyperfilm ECL (GE Healthcare) ...