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Citations for GE Life Sciences :
3051 - 3100 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The proteins were further purified by size exclusion chromatography (SEC; HiLoad 16/600 Superdex 200 pg, GE Healthcare) in 50 mM phosphate pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... GST-Bro1V fusion protein and mutants thereof were purified using the Glutathione Sepharose 4B (GE Healthcare, United Kingdom), treated with AcTEV protease (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were further purified by size exclusion chromatography (Superdex-75 10/300 GL column; GE Life Sciences) and judged greater than 95% pure by SDS-PAGE analysis.
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bioRxiv - Cell Biology 2022Quote: ... and 0.1 mg protein were loaded on a Superdex 200 10/300 GL size-exclusion column (GE Healthcare) connected to an ÄKTA pure (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... Labeled proteins were separated from free dye molecules by gel filtration using a Superdex 200 column (GE Healthcare)56.
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bioRxiv - Biochemistry 2022Quote: ... Concentrated protein for Cryo-EM was loaded over a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) using SEC Buffer (25mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: ... Then the protein was purified by gel-filtration chromatography using a Superose 6 gel filtration column (GE Healthcare) pre-equilibrated with HBS buffer (10 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Then the proteins were purified from the lysate with the Ni2+-NTA column of ÄKTA Pure (GE healthcare). Then the proteins were biotinylated using Avidity biotinylation kit and recommended protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was diluted with lysis buffer before application to a 5ml HiTrap STREP column (GE Healthcare), washed with lysis buffer and eluted using buffer supplemented with 2 mM Desthiobiotin ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was concentrated and applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) in the same buffer.
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bioRxiv - Immunology 2022Quote: IgG was purified by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences, 17040203) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins of interest were visualized with the Amersham ECL Detection Reagents (GE Healthcare by SIGMA, Cat. No. RPN2105) or LiteAblot TURBO Extra-Sensitive Chemioluminescent Substrate (EuroClone ...
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bioRxiv - Microbiology 2022Quote: ... The protein was then further purified by gel filtration using Superose 6 increase 10/300 column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein solution was run at room temperature on a Superdex 75 10/300 GL (GE Healthcare, Piscataway, NJ) column with 2 mg/ml gD1 and a mobile phase composed of Tris- HCl at pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was run over a size exclusion column (GE Healthcare HiPrep 26/60 Sephacryl S-300 HR) in sizing buffer (50 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... protein digestion was concentrated and applied to a HiLoad™ 16/600 Superdex™ 75pg column (GE Healthcare) in 20 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... 500 µl of freshly purified protein was loaded on a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... The P5CS proteins were further purified through HiLoad 16/600 Superdex 200 pg gel-filtration chromatography (GE Healthcare) in column buffer (25 mM HEPES pH 7.5 and 100 mM KCl) ...
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bioRxiv - Plant Biology 2021Quote: ... the purified protein was loaded on an analytical size exclusion column (SEC, superdex200 10/300, GE Healthcare, USA) pre-equilibrated with buffer containing 25mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: The purified protein was analyzed by Size Exclusion Chromatography (SEC) (Superdex 200 10/30 GL column; GE Healthcare) coupled with multi-angle light scattering (MALS ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare Life Sciences, Waukesha, WI), which were incubated at 4°C overnight with primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... DNA fragments encoding APC proteins were inserted into pGEX-5X-1 (GE Healthcare Life Sciences, Pittsburgh, PA, USA). For in vitro transcription and translation reaction ...
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bioRxiv - Cell Biology 2020Quote: ... Protein transfer from gel to membrane was performed with a 0.45 μm PDVF AmershamTM HybondTM membrane (GE Healthcare, formerly Amersham Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved DEPTOR protein was then collected and applied to a 5 mL HiTrap Heparin HP column (GE Healthcare) equilibrated with HEP-A buffer (50 mM HEPES pH 8 ...
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bioRxiv - Biophysics 2021Quote: ... The protein was then injected on a High load superdex 200 16/600 gel filtration column (GE Healthcare) in deuterated PBS buffer pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... The protein was further purified via gel filtration using Superose 6 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with the purification buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was divided equally and incubated with 50 μl of prewashed 50% Protein G slurry (GE Healthcare) for each condition ...
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bioRxiv - Immunology 2022Quote: ... Protein was concentrated and further purified by size-exclusion chromatography using Superose 6 16/60 (GE Life Sciences) in a PBS buffer ...
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bioRxiv - Immunology 2022Quote: ... Protein was further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... 5% glucose) and the proteins purified by affinity chromatography using a HiTrap Heparin High-Performance column (GE Healthcare) and gradient elution (0-100% NaCl ...
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bioRxiv - Cell Biology 2022Quote: All chromatography steps were performed at 4°C using ÄKTAxpress or ÄKTA pure protein purification systems (GE Healthcare). Firstly ...
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bioRxiv - Cell Biology 2022Quote: ... All proteins were further purified by gel filtration on a Superdex S200 preparative or analytical column (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... the labeled KRAS protein was purified by size-exclusion chromatography using 16/60 Superdex75 increase column (GE Healthcare) pre-equilibrated with 20 mM HEPES (pH 7.0) ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
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bioRxiv - Microbiology 2020Quote: ... the two proteins were mixed together in solution and purified using a Superdex200 gel filtration column (GE Healthcare). The complex was concentrated to 10 mg/ml in buffer 20 mM Tris pH 7.2 and 200 mM NaCl ...
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bioRxiv - Pathology 2020Quote: ... wt:wt) was determined by size exclusion chromatography in AKTA Primer Plus fast protein liquid chromatography system (GE Healthcare) with Superose™ 6 10/30 GL Column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... run at a flow rate of 0.5-1 mL/min on Äkta Start protein purification system (GE Healthcare). Antibodies were eluted using 0.1M glycine pH 2 ...
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bioRxiv - Biochemistry 2020Quote: ... This protein was applied to a 16/600 Superdex 75 preparative size exclusion column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... Target protein was further purified by passage through a HiLoad 16/600 Superdex 75 pg (GE Healthcare, USA) in buffer E (20 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... The proteins were detected using the Amersham ECL Prime Western Blotting Detection Reagent (RPN2232, GE Healthcare Life Sciences) and visualized on the ECL Chemocam Imager (INTAS) ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare), and purity was confirmed to be at least 90% by SDS polyacrylamide gel electrophoresis ...
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bioRxiv - Synthetic Biology 2021Quote: ... Concentrated protein was loaded onto a HiLoad 26/60 Superdex 200 prep grade column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris ...
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bioRxiv - Synthetic Biology 2021Quote: ... Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated and further purified using an S200 16/60 gel filtration chromatography column (GE Healthcare) previously equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein bands were detected with the enhanced chemiluminescence method (FUJIFILM Wako) and chemiluminescence film (GE Healthcare, Buckinghamshire, UK), and target protein levels were analyzed using NIH ImageJ software.
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bioRxiv - Microbiology 2020Quote: ... protein bands were visualized by chemiluminescence and the image was captured by ImageQuant LAS 4000 mini (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... Protein samples were resolved on the SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare). The membrane was blocked with 5% non-fat dry milk in tris-buffered saline ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Proteins of interest were visualized on a Bio-Rad ChemiDoc MP imager with Enhanced Chemiluminescence Plus (GE Healthcare) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: Cell lysates (10μg protein) were resolved by electrophoresis and transferred to a polyvinylidene fluoride membrane (GE Healthcare, UK). Membranes were blocked for 1 hour in 5% skimmed milk/TBS/0.1% Tween-20 and incubated with primary antibodies (1:1000 dilution ...