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Citations for GE Life Sciences :
2751 - 2800 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing 2F2 or 2E10.E9 IgG was purified via Protein G affinity chromatography (GE Healthcare) and size-exclusion chromatography (Superose 6 Increase 10/300 GL ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted and further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The deglycosylated proteins were then purified by batch affinity chromatography on Glutathione Sepharose 4B resin (GE Healthcare) followed by SEC ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 μL lysate (2 mg protein) was injected through a 0.75mm loop (GE Healthcare 18-1112-53). Run parameters were 0.5 mL/min and 1.5MPa for 1 column volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μL of protein was injected onto a Superdex 75 increase 10/300 GL column (GE Healthcare) pre-equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were purified by Ni-NTA IMAC using Ni-Sepharose 6 Fast Flow resin (GE Healthcare). The wash buffer consisted of 50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin was precleared for 1 hour at 4 °C with blocked protein A Sepharose beads (GE Healthcare, Eindhoven ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with 0.5 ml protein-A-sepharose (nProtein A Sepharose 4 Fast flow, GE healthcare #1705280) at 4 °C for 24 hr ...
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bioRxiv - Biophysics 2022Quote: ... ion exchange chromatography for initial protein purification was performed on a 1ml HiTrap SP FF (GE healthcare) and a 24ml Superdex 75 10 300 GL (GE healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... was used to visualize protein bands using an ImageQuant LAS4000 imager (GE Healthcare #28955810, Chicago, Illinois, USA).
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected from selected hybridoma clones and purified through a protein G-sepharose column (GE Healthcare). mAbs were isotyped with Pierce rapid isotyping kit (Cat#26178) ...
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bioRxiv - Biochemistry 2020Quote: ... Purified protein was transferred to appropriate buffers by size exclusion chromatography (Superdex 75 10/300, GE Healthcare) and stored at 4°C.
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bioRxiv - Microbiology 2020Quote: ... The buffer was filtered (0.2 µm filters) prior to protein separation on an Äkta Purifier (GE Healthcare). The fractions containing RNA polymerase were pooled and dialyzed against RNA polymerase storage buffer (50 mM Tris–HCl ...
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bioRxiv - Microbiology 2020Quote: ... HR1-L6-HR2 protein was concentrated and gel-filtered on a 10/300 Superdex 75 (GE Healthcare) column ...
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bioRxiv - Genomics 2020Quote: ... Eluted protein samples were further purified by gel filtration using Superose 6 10/300 column (GE Healthcare). These proteins were buffer-exchanged to storage buffer containing 20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the purified protein were pooled and desalted with PD midi Trap G-25 (GE Healthcare) using the spin protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Protein solution was loaded onto a HisTrap HP column connected to a GSTtrap FF column (GE Healthcare) equilibrated in buffer A to remove the GST-tagged PreScission protease ...
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bioRxiv - Bioengineering 2021Quote: ... In vitro translated proteins were visualized by autoradiography using Typhoon FLA 9500 Phosphorimager (GE Healthcare Life Sciences). Signal intensity (corrected for background signal ...
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bioRxiv - Biophysics 2020Quote: ... Protein samples were freshly prepared using size exclusion chromatography (GE Life Sciences, Superdex 75 10/300 GL) in a buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... Proteins of interest were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (GE Healthcare). Peroxidase substrate was provided by ECL Western Blotting Substrate kit (Pierce) ...
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bioRxiv - Immunology 2021Quote: ... Proteins were concentrated in centrifugal concentrators and purified by size-exclusion chromatography (Superdex 200; GE Life Sciences) in gel filtration buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... Proteins were purified as above using Ni2+ affinity and size exclusion chromatography (Superdex 75, GE Life Sciences), excluding cleavage of the His6-tags.
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bioRxiv - Immunology 2021Quote: ... The dialyzed protein was further purified on a pre-equilibrated Superdex 75 gel filtration column (GE Healthcare), and fractions containing monomeric soluble NLRP3PYD were concentrated to 1 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... and loaded on superdex 75 column on an AKTA pure 25L protein purification system (GE Healthcare, USA) in 20 mM HEPES pH 7.5 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... the proteins were further purified using a Hiload 16/600 Superdex 200 pg gelfiltration column (GE Healthcare) in SEC Buffer (20 mM HEPES/NaOH pH7.8 and 250 mM NaCl) ...
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bioRxiv - Biophysics 2021Quote: ... The protein purification was carried out using a cation exchange HiTrap SP column (GE Healthcare, Chicago, IL) with phosphate buffer (25 mM sodium phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further purified by size-exclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare) column.
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bioRxiv - Biochemistry 2021Quote: ... The protein sample (50 μL) was loaded into a 10/300 Superdex® 200 GL (GE Healthcare) column connected to the MALS detection unit for SEC ...
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bioRxiv - Biochemistry 2020Quote: ... Concentrated protein was then applied to a size exclusion column (HiLoad 16/60 Superdex 200, GE Healthcare) pre-equilibrated with Buffer GF (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The protein complex eluted as a monodisperse peak from a Superdex 200 gel filtration column (GE Healthcare) in 20 mM HEPES/NaOH pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were further purified using Superdex 75 16/60 or Superdex 200 16/60 (GE Healthcare) gel filtration chromatography and stored in their respective running buffers ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated and run over a HiLoad 16/600 superdex 75 gel filtration column (GE Healthcare) using an AKTA pure (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... 0.3 M NaCl using a Fast Protein Liquid Chromatography (FPLC) system (ÄKTA Purifier, GE Healthcare Life Sciences). Prior to loading ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were further purified using 16/600 Superdex 75 pg size-exclusion chromatography columns (GE Healthcare) equilibrated in protein buffer (20mM Tris pH 7.8 ...
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bioRxiv - Neuroscience 2020Quote: ... Immune complexes were isolated by the addition of 20 μL of protein G– Sepharose beads (GE Healthcare) followed by incubation for 2 hr at 4°C ...
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bioRxiv - Biophysics 2021Quote: Purified protein or cell lysates were injected onto a Superose 6 increase 5/150 column (GE Healthcare), running in a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was further purified by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 50 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... The reconstituted protein was further purified on the Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in a buffer comprising 20 mM Bis-Tris pH 6.8 ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing protein of interest were collected and desalted using PD-10 midi desalting columns (GE Healthcare). Protein of interest was eluted with the storage buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibodies and dilutions used were ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibody and dilution used was rat anti-HA-HRP 3F10 (Roche Applied Science) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibodies and dilutions were ...
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bioRxiv - Cell Biology 2021Quote: ... Labeled proteins were purified by final buffer exchange using size exclusion chromatography (Superdex 200 column, GE Healthcare) in the appropriate buffer (i.e ...
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bioRxiv - Microbiology 2020Quote: ... The concentrated DnaA protein was subjected to size exclusion chromatography on an Äkta pure instrument (GE Healthcare). 200 μL of concentrated DnaA-6xHis was applied to a Superdex 200 Increase 10/30 column (GE Healthcare ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were detected by Enhanced Chemiluminescence (ECL) using ECL Western Blotting reagents (Amersham, GE Healthcare, UK) or SuperSignal West Dura Extended Duration Substrate (Pierce ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... denatured proteins were then separated via SDS-PAGE and transferred to a PVDF membrane (GE Healthcare, Amersham). Blots were preincubated in a blocking solution of 5% BSA in 0.2% TBST (0.1 M Tris base ...
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bioRxiv - Plant Biology 2020Quote: ... the purified RCA protein was immobilized on a carboxy-methylated dextran sensor chip (CM5) chip (GE Healthcare) using an amine coupling method kit (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin samples derived from 1.0×106 cells were precleared with protein G sepharose (GE Healthcare, Chicago, IL) and then 1-5 μg of each antibody was added to form immune complex ...
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bioRxiv - Microbiology 2020Quote: ... supernatant containing VSG protein was loaded onto an anion-exchange column (Q-Sepharose Fast-Flow, GE Healthcare), which had been equilibrated with 10 mM phosphate buffer ...