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Citations for GE Life Sciences :
251 - 300 of 324 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... the solution was loaded onto a CM-Sepharose column coupled to an Äkta Pure system (GE Healthcare Life Sciences, Brøndby, Denmark). The sample was loaded on a column equilibrated with buffer A (50 mM Tris-HCL ...
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bioRxiv - Immunology 2021Quote: ... The protein was eluted with 10 mM maltose solution in the lysis buffer and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES at pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Aliquots of solution taken at various time intervals (0 – 390 mins) were loaded to a 5mL HiTrap Q HP column (GE Healthcare) equilibrated in buffer (20 mM HEPES pH 7.0 ...
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bioRxiv - Bioengineering 2021Quote: ... we typically added 20 mL leukocyte products on top of 20 mL Ficoll-Paque solution for density gradient centrifugation from GE Healthcare in a 50 mL Falcon tube and then centrifuged at 800× g at room temperature for 20 min to separate different leukocyte cells ...
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bioRxiv - Microbiology 2022Quote: ... The infusions were stored over-night at 4°C before the leaves were removed and the aqueous solutions were sterile-filtered (200 μM filter, Whatman/GE Healthcare). Afterwards ...
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bioRxiv - Immunology 2022Quote: ... The medium of samples collected from the Histrap columns was substituted with PBS solution and then further purified with the Superdex 200 column (GE Healthcare). Lastly ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Microbiology 2022Quote: ... Erythrocyte lysis was performed using ammonium-chloride-potassium (ACK) buffer and leukocytes were isolated by gradient centrifugation using Percoll solution (GE Healthcare). Lung tissue leukocytes were filtered again and antibody staining for flow cytometry was performed.
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence solution (MoBiTec) were used for visualization on an Amersham Imager 680 (GE Healthcare) or a Cawomat 2000 IR apparatus (CAWO solutions).
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bioRxiv - Biochemistry 2021Quote: ... The buffer salt concentration was diluted to 30 mM NaCl and the solution was flown through a 5 mL HiTrap Q HP (GE Healthcare) and a NiNTA column ...
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bioRxiv - Biophysics 2020Quote: ... Fixed cells were rinsed three times in warm PBS and incubated 30 min with a blocking solution containing 1% BSA (Bovine Serum Albumine, GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Plant Biology 2020Quote: ... the membranes were incubated for 2 h in Can Get Signal Solution 2 (Toyobo) with anti-rabbit or mouse IgG conjugated to horseradish peroxidase (GE Healthcare). After washing twice with TBST ...
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bioRxiv - Cancer Biology 2020Quote: ... the reaction was quenched by addition of the same EDTA solution and the labeled construct was purified using gel-filtration chromatography (Sephadex G-25, PD10 desalting column; GE Healthcare) into 0.9% saline ...
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bioRxiv - Biophysics 2020Quote: ... 100 μL of this peptide solution was subjected to SEC analysis by passing through Superdex peptide 10/300 GL column (GE healthcare) connected to Bio-Rad (Biologic Duoflow ...
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bioRxiv - Immunology 2020Quote: ... An equal volume of buffer (2 mM EDTA in PBS) and blood was mixed and carefully layered over 5ml Ficoll Hypaque solution (GE Healthcare). After centrifugation for 20 min at 900 g (with no break ...
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bioRxiv - Immunology 2020Quote: ... the surface was saturated with two sequential injections of a 20 μM knob domain solution in HBS-EP buffer (GE healthcare), using a flow rate of 30 μL/min and contact time of 300 seconds ...
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bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
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bioRxiv - Neuroscience 2024Quote: ... and the bands were detected using an immunoreaction enhancing solution (Can Get Signal; Toyobo) and enhanced chemiluminescence (ECL Prime; GE Healthcare). Chemiluminescence signals were digitized using a ChemiDoc MP imaging system (Bio-Rad Laboratories) ...
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... and the bands were detected using an immunoreaction enhancing solution (Can Get Signal; Toyobo) and enhanced chemiluminescence (ECL Prime; GE Healthcare).
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bioRxiv - Plant Biology 2024Quote: ... the cleaved tag from the 6xHis-MBP-Pwl2 purification was removed from the solution using a MBPTrap HP dextrin sepharose column (GE Healthcare) and subsequently eluted using SEC buffer supplemented with 10mM maltose.
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bioRxiv - Molecular Biology 2024Quote: ... The final solution was then purified to greater than 95 % purity over a Sephadex S-300HR gel filtration column (GE Healthcare) and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The final solution was then purified to greater than 95 % purity over a Sephadex S-300HR gel filtration column (GE Healthcare) and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) pre-equilibrated with the HiTrap binding buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with blocking solution (2.5 % FBS, 200 mM glycine, 0.1 % Triton X-100 in PBS or 2 % bovine serum albumin (GE Healthcare or Calbiochem), 0.01 % Tween 20 (National Diagnostics or Roth ...
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bioRxiv - Biophysics 2022Quote: Working solutions for light scattering were filtered through Whatman® 0.02 μm aluminum oxide filters (GE Healthcare Bio-Sciences, Pittsburgh, PA). All polystyrene bottles (Nalgene ...
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bioRxiv - Biophysics 2023Quote: ... The fixed cells were rinsed three times in warm PBS and incubated for 30 min with a blocking solution containing 1% BSA (GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Biophysics 2023Quote: ... The solution was concentrated to ∼1 ml and went through gel filtration on Superdex 75 10/300 increase column (GE Healthcare) equilibrated with a buffer containing 20 mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were isolated from peripheral blood samples of a healthy volunteer by density gradient centrifugation using Ficoll-Paque solution (GE Healthcare) and were freshly transferred to NSG mice ...
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bioRxiv - Bioengineering 2023Quote: ... where 18 mL of blood was mixed with Hanks’ Balanced Salt Solution (HBSS, 14175095, GibcoTM) before being poured onto Ficoll-Paque Plus (GE17-1440-03, GE Healthcare). The layers were separated through centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... from 3 different healthy donors to avoid unique donor bias on type cells effects and extracted using Ficoll-Plaque Plus solution (GE Healthcare). PBMCs were re-suspended in RPMI-1640 medium (Lonza Bioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... 140∼150 uL of 6∼7 mg/mL of each complex solution was injected into the superdex200 increase 10/300 (GE Healthcare) column ...
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bioRxiv - Immunology 2024Quote: ... Samples were centrifuged at 220xg for 10 min at 4°C and then resuspended in a 22% Percoll solution (GE Healthcare) with a PBS layer floated on top ...
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bioRxiv - Systems Biology 2024Quote: ... Digests were filtered through 100 μm mesh strainer (Falcon) and subjected to density gradient centrifugation using 40% and 70% Percol solutions (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... A 25 mL aliquot was layered over a 1.073 g·mL−1 Ficoll-Paque™ PLUS solution (GE Healthcare, Chicago, Illinois, United States) and centrifuged at 2000 x g for 25 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were treated with oxygen plasma for 2 min and immediately submerged in Plus-One Repel Silane solution (GE Life Sciences) for 3 min ...
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bioRxiv - Neuroscience 2020Quote: ... 20–40 g) and placed on ice in Petri dishes containing 1X Hank’s Balanced Salt Solution (HBSS, SH30588.02, GE Healthcare Life Sciences, US). Ganglia were minced using surgical micro scissors and incubated in a papain solution (32 μL papain (27.3 U/mg ...
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bioRxiv - Cell Biology 2022Quote: ... for 2.5 hours at 37 °C and 13mm coverslips were silanized for 3 minutes using ethanol containing 0.37% Bind-Silane solution (GE Healthcare Life Science) and 3.2% acetic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... The gel was stained with ethidium bromide solution for 25 minutes followed by visualization on a Typhoon FLA 9500 fluorescence imager (GE Life Sciences) by selecting Cy3 scanning mode ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated by density gradient centrifugation using the Ficoll® Paque Plus solution (17-1440-02, GE Healthcare).
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were then washed in PBT and incubated with HRP conjugated-anti-rabbit IgG (GE Healthcare # NA9340, 1:5000 in blocking solution) at room temperature for 1 hour and washed twice in PBT ...
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bioRxiv - Cell Biology 2023Quote: ... The eluate fraction was dried using a vacuum centrifuge and resuspended in 3.6 mL ampholyte solution (pI 3-10, GE Healthcare, Chicago, IL). Offgel fractionation was performed using a 3100 OFFGEL fractionator (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved His6-SUMO tag and uncleaved His6-SUMO-GBP2 were separated from tag free GBP2 by passing the solution over a His-Trap column (GE Healthcare/Cytiva). Tag free GBP2 in the flow-through was concentrated and subjected to size exclusion chromatography on a HiLoad16/600 Superdex 200pg column (GE Healthcare/Cytiva ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were injected two times/embryo with mRNAs at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNAs or plasmid DNA at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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Improved Activity and Kinetics of Endoglucanase Biofuel Enzyme with Addition of an AzoTAB SurfactantbioRxiv - Bioengineering 2023Quote: ... The protein solution was filtered and applied to a 5 mL HiTrap Q HP anion exchange column (GE Healthcare, Cat. no. 17115301) and later washed with 30× column volume with the protein buffer ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... The resulting solutions were then filtered using a 0.22 μm filter unit and applied to a Ni2+-sepharose resin (GE Healthcare, fast flow) pre-equilibrated with binding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The gel was stained with Sybr Gold solution for 25 minutes followed by visualization on a Typhoon FLA 9500 fluorescence imager (GE Life Sciences) by selecting Cy3 scanning mode ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 20 minutes then incubated in an excess of blocking solution (1% bovine serum albumin, Sigma-Aldrich, A7906; 1% inactivated horse serum, GE Healthcare B15-023 ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were injected with mRNAs at four-cell to eight-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...