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Citations for GE Life Sciences :
201 - 250 of 324 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... the labeled protein was separated from the solution by size exclusion chromatography using PD MiniTrap G25 (GE Healthcare). PLCγ1 nSH2-cSH2-SH3 and truncations were labeled with CoA 647 (NEB ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were resuspended in 40% Percoll solution and overlayed on 70% Percoll (#17-0891-01, GE Healthcare). After a density centrifugation step (at 2100 rpm ...
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bioRxiv - Biophysics 2021Quote: ... and the final product was obtained by running the solution through an 5 mL Hi-Trap (GE Healthcare) desalting column (Figure S1).
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies were diluted in blocking solution at 1:1000 (Anti-rabbit IgG: GE Healthcare Cat#NA934, RRID:AB_772206) and 1:2000 (Anti-mouse IgG ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein solution was run at room temperature on a Superdex 75 10/300 GL (GE Healthcare, Piscataway, NJ) column with 2 mg/ml gD1 and a mobile phase composed of Tris- HCl at pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... the α-Syn solution was subjected to anion exchange column (AEC) (HiTrap Q Hp 5 ml, GE healthcare) followed by size exclusion chromatography (SEC ...
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bioRxiv - Physiology 2021Quote: ... The blots were visualized with enhanced chemiluminescence solution (ECL:Prime Western Blotting Detection Reagent, GE Healthcare, Piscataway, NJ, USA) and scanned with a densitometer (LAS-1000 ...
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bioRxiv - Microbiology 2020Quote: ... the two proteins were mixed together in solution and purified using a Superdex200 gel filtration column (GE Healthcare). The complex was concentrated to 10 mg/ml in buffer 20 mM Tris pH 7.2 and 200 mM NaCl ...
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bioRxiv - Biophysics 2020Quote: ... The solutions were then purified using a Superdex 75 Increase 10/300 GL column (GE Healthcare, Chicago, USA) at a flow rate of 0.5 ml/min and eluted in 20 mM sodium phosphate buffer (pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Then the solution was injected onto a prewashed SP Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL). The column was washed with 20 CV of Buffer D (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The dialyzed solution was loaded on a Superdex 75 pg 16/60 size-exclusion column (GE Life Sciences) using buffer C as a running buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed solution was then loaded on a 1 ml HisTrap SP FF column (GE Healthcare, Pittsburgh, PA) and eluted using a linear gradient of NaCl (20-500 mM) ...
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bioRxiv - Biochemistry 2023Quote: ... The evaporated solution was centrifuged as described above and applied to a QAE Sephadex A25 column (GE Healthcare) equilibrated with 500 ml of 50 mM Tris/HCl pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were subjected to a density gradient centrifugation using a 37% Percoll solution (GE Healthcare Life Sciences). Erythrocytes were lysed with NH4Cl.
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bioRxiv - Biophysics 2022Quote: ... The solution was immediately desalted and the buffer exchanged by passing it over a Sephadex G25 (GE Healthcare) XK 50/20 column equilibrated with 20 mM MES-NaOH pH 5.5 ...
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bioRxiv - Microbiology 2023Quote: ... the solution was applied to a size exclusion chromatography (SEC) on a HighLoad 75 Superdex column (GE Healthcare) equilibrated with buffer D ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein solutions was loaded on to Superose 6 Increase 10/300 GL column (GE Healthcare, Madison, WI) that was equilibrated with a buffer containing 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Biochemistry 2024Quote: ... The dialyzed solution was then loaded on a 5 ml HiTrap SP FF column (GE Healthcare, Pittsburgh, PA) and eluted using a linear gradient of NaCl (20-500 mM) ...
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bioRxiv - Cell Biology 2020Quote: ... Umbilical cords were flushed with Hank’s balanced salt solution with magnesium and calcium (HyClone™, GE Healthcare, Solingen, Germany) and PBS with magnesium chloride and calcium chloride (Merck KGaA ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were detected against mouse or rabbit IgGs and visualized with ECL Western blot detection solution (GE Healthcare) or Odyssey infrared imaging system (LI-COR ...
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bioRxiv - Biochemistry 2022Quote: ... The products derived from CGN12 reporter gene were detected by homemade ECL solution using the ImageQuant LAS4000 (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The solution was added to 250 μL (bed volume) Protein G Sepharose™ 4 Fast Flow resin (GE Healthcare) prewashed with DPBS ...
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bioRxiv - Microbiology 2022Quote: ... The solution containing the peptide was applied to a HiPrep SP FF 16/10 column (GE Healthcare Life Sciences) equilibrated with 20 mM sodium phosphate buffer at pH 3.9 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The filtered solution was then purified by Ni2+ affinity chromatography using a 1 mL HisTrap FF column (GE Healthcare). The column was equilibrated with 25 mL Buffer A (20 mM sodium phosphate (pH 7.4) ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunostained embryos were mounted in clearing solution Omnipaque (350 mg litre−1 concentration per 1 ml iohexol, GE Healthcare) and imaged using Leica TCS SP8 DLS microscope as described above ...
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bioRxiv - Biochemistry 2023Quote: ... 200 μL of protein solution were loaded onto a Superdex 200 HR 10/30 gel filtration column (GE Healthcare) with a flow rate of 0.5 ml/min ...
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bioRxiv - Neuroscience 2023Quote: ... and collections of the flowthrough were centrifuged prior to loading the solution onto a HisTrapTM HP column (GE Healthcare). The column was washed with buffer 1A (20 mM imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... the protein solution was concentrated and further purified utilizing size exclusion chromatography with a SuperdexTM 75 column (GE Healthcare). The protein solution was concentrated to 9.2 mg/mL and used for crystallization.
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bioRxiv - Cancer Biology 2022Quote: ... in Can Get Signal Solution 2 (Toyobo) and visualized with ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232). Chemiluminescent signals were detected using a LAS-3000 Imaging System (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... The solution was then concentrated by crossflow filtration and passed over a Superdex 200 26/60 column (GE Healthcare) equilibrated in 6 M GdmCl ...
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bioRxiv - Biophysics 2022Quote: ... The solution was then concentrated by crossflow filtration and passed over a Superdex 200 26/60 column (GE Healthcare) equilibrated in 6 M GdmCl ...
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bioRxiv - Synthetic Biology 2022Quote: ... The filtered solution was then purified by Ni2+ affinity chromatography using a 1 mL HisTrap FF column (GE Healthcare). The column was equilibrated with 25 mL Buffer A at a constant flow rate of 1 mL/min ...
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bioRxiv - Genomics 2023Quote: ... DNA was suspended in 50 µl of the hydration solution using a Tissue DNA isolation kit (GE HealthCare LifeSciences).
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bioRxiv - Biochemistry 2020Quote: ... The solution was dialyzed against Buffer C overnight at 4 °C and applied on a Resource Q column (GE Healthcare) that was equilibrated with Buffer C ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Cell Biology 2021Quote: ... 76 mm × 24 mm super frost glass slides were coated with a solution made of 14:1:1 ethanol:acetic-acid:PlusONE Bind-Silene (GE Healthcare; vol:vol:vol). In parallel ...
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bioRxiv - Biophysics 2021Quote: ... To remove aggregated protein the protein solution was filtered through a 0.2 µm filter and loaded on a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer for size-exclusion chromatography ...
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bioRxiv - Pathology 2021Quote: ... the crude protein solution was finally collected by ultracentrifugation (40,000 rpm, 30 min, 4°C) and subsequently incubated with Ni-NTA (GE healthcare) resin for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The solution was then applied to a HisTrap FF crude column on an Aekta pure system (GE Healthcare, Freiburg, Germany), extensively washed with His-A buffer ...
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bioRxiv - Neuroscience 2022Quote: ... hippocampi were subjected to enzymatic digestion in Hanks′ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with Trypsin (1x ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein solution is again filtered before being loaded onto an equilibrated Superdex 200 size exclusion column (GE Healthcare) to separate the FMRP constructs from the Ulp protease and the His-SUMO fusion tag ...
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bioRxiv - Molecular Biology 2020Quote: The state of XynA in solution was analyzed by a Superdex 200 (10/300) increase column (GE Healthcare, United States). First ...
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bioRxiv - Plant Biology 2021Quote: ... was added to the supernatant to dilute the ammonium sulfate concentration to 0.5 M and the solution was applied to pre-equilibrated hydrophobic column after passing through a 0.22 μm filter (Hitrap phenyl, GE Healthcare). Proteins were eluted by an ammonium sulfate gradient from 0.5 M to 0 M with ten column volumes ...
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bioRxiv - Biophysics 2021Quote: ... aSyn in Tris was buffer exchanged into freshly prepared NaCl and CsI solutions (pH 5.4) before experiments using PD10 dialysis columns (GE Healthcare). Protein concentration was determined from the absorbance measurement at 280 nm on a Nanovue spectrometer using the extinction coefficient for aSyn of 5960 M−1cm−1.
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bioRxiv - Biochemistry 2021Quote: ... 100 μL solution of 1.5 mg/mL protein was injected onto a Superdex 200 Increase 10/300 GL (GE Healthcare) column equilibrated in a buffer of 20 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... The solution was filtered and the model degrons were loaded on a HiTrap SP HP 5 ml column (GE Healthcare) in 50 mM ammonium acetate pH 4.5 ...
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bioRxiv - Biophysics 2022Quote: ... Excess unreacted BADAN was removed by gel filtration by passing the reaction solution through a PD-10 column (GE Healthcare). Labeling efficiency was measured by UV-visible spectroscopy based on extinction coefficients of SLO-1 (ε280 = 132 mM-1 cm-1 ...
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bioRxiv - Cell Biology 2023Quote: ... The solution was then passed over a Superose 6 Increase 10/30 size-exclusion chromatography column (GE Healthcare Life Sciences) that was preequilibrated in 50 mM phosphate buffer pH 7.5 ...
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bioRxiv - Physiology 2023Quote: ... the membranes were then washed three times with a PBS 0.3% tween solution and incubated with a horseradish peroxidase-conjugated secondary antibody (GE Healthcare) in PBS 0.3% tween 3% BSA for one hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were suspended in 30 µL of an aequous solution containing Tris (50 mM, GE Healthcare, Chicaco, IL, USA), NaCl (150 mM ...