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Citations for GE Life Sciences :
51 - 100 of 324 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Regeneration was initiated by 50 mM NaOH solution (GE Healthcare). Obtained SPR sensorgrams were fitted with a 1:1 Langmuir binding model using BIAcore evaluation software ...
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bioRxiv - Cancer Biology 2024Quote: ... Penicillin/Streptomycin Solution (cat# SV30010) was purchased from GE Healthcare. EGF (cat# PHG0311 ...
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bioRxiv - Cancer Biology 2024Quote: ... The blots were developed using enhanced chemiluminescence solution (GE Healthcare). To estimate the molecular weight of the analyzed proteins PageRuler Prestained Protein Ladder (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... the membrane was submerged in ECL developing solution (GE Healthcare) and the image was captured on X-ray film ...
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bioRxiv - Biochemistry 2021Quote: Fractions containing the dIG8-CC protein were then buffer-exchanged to buffer B (20mM Tris·HCl, 150 mM sodium chloride, pH 7.5) in a HiPrep 26/10 desalting column (GE Healthcare), and incubated overnight at 4°C with inhouse-produced His6-tagged TEV peptidase at a peptidase:substrate ratio of 1:20 (w/w ...
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bioRxiv - Immunology 2021Quote: ... Membranes were washed and coated with ECL substrate solution (GE Healthcare) before imaging on a ChemiDoc XRS+ (BioRad) ...
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bioRxiv - Immunology 2021Quote: ... Leukocytes were enriched using 37.5% Percoll solution (GE Healthcare, Uppsala, Sweden)) and red blood cells lysed with Ammonium-Chloride-Potassium (ACK ...
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bioRxiv - Plant Biology 2021Quote: ... Rinsed membranes were then treated with ECL Prime solution (GE Healthcare) and luminescence imaged with an ImageQuant LAS 4000 system (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... the solution was filtered (0,2 µm PTFE membrane, GE Healthcare, France) and directly added to fluorophore amines at 1.7 eq ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were imaged using Amersham ECL prime solution (GE Life Sciences) and a Fusion Pulse 6 imager (Vilber Lourmat) ...
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bioRxiv - Immunology 2020Quote: ... Complexes were detected with the chemiluminescent substrate solution ECL (GE Healthcare).
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bioRxiv - Pathology 2021Quote: ... signals were detected with ECL solution (GE Healthcare, Little Chalfont, UK). Protein levels on the membrane were quantified by densitometry using Gel-Doc software (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting protein solution was purified by Histrap column (GE Healthcare) and then buffer exchanged into DPBS by using desalting column (Thermo) ...
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bioRxiv - Cancer Biology 2023Quote: ... Western blots were developed with freshly mixed ECL solutions (GE Healthcare). The antibodies used are the following ...
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bioRxiv - Molecular Biology 2022Quote: ... The solutions were loaded onto a Mono Q column (GE Healthcare). Proteins were eluted with a linear gradient to 60% Buffer C (50 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... After centrifugation cells were resuspended in 37.5% Percoll-solution (GE Healthcare) and centrifuged for 5min at 1800rpm ...
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bioRxiv - Genomics 2024Quote: ... Western blots were developed with freshly mixed ECL solutions (GE Healthcare). The following antibodies were used ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein sample was dialyzed against 20 mM Tris-HCl (pH 8.0) and 2 mM CaCl2 (buffer B) and applied to a Mono Q 10/100 GL column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Immunology 2020Quote: ... running on a mobile phase buffer of buffer A (0.1% trifluoroacetic acid (TFA)) and buffer B (80% acetonitrile/0.1% TFA) on an AKTAmicro™ HPLC system (GE Healthcare). The separation was performed with a rapid gradient of buffer A to B (2 to 40% B for 4 mins ...
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bioRxiv - Biochemistry 2020Quote: ... using a linear gradient of buffer B to increase the concentration of imidazole on an ÄKTA purifier system (GE Healthcare). The protein was concentrated and further purified using an S200 16/60 gel filtration chromatography column (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: Ultrasonography images of the vastus lateralis (VL) were acquired with a B-mode imaging device (LOGIQ S7 Expert, GE Healthcare) using a multi-frequency linear array transducer (3-12 MHz ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... 50 mM HEPES pH 7.5 (Buffer B) supplemented with 30 mM imidazole before elution directly into a PD-10 column (GE Healthcare; equilibrated in Buffer B + 20 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... The heparin column containing the bound protein was washed with 25 mL of 10% buffer B and the protein was eluted using linear gradient of buffer A and B (10-100%) over 45 min with a flowrate of 1.5 mL/min using an AKTA FPLC instrument (GE Healthcare). The fractions containing pure MBP-MUTYH protein were analyzed by SDS-PAGE and concentrated using Amicon ultracentrifugation filters (30,000 MWCO) ...
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bioRxiv - Immunology 2021Quote: ... The column was washed with Buffer A and the protein eluted with a gradient to Buffer B (500 mM NaCl, 20 mM NaH2PO4, 500 mM imidazole) on an ÄKTA Pure chromatography system (GE Healthcare). Fractions containing the protein were pooled ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions were collected and diluted with buffer B to a conductivity of 18 mS/cm and loaded onto HiTrap SP columns (GE healthcare). A linear gradient of buffer B containing 1 M NaCl was used for elution ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was additionally diluted with buffer B to a conductivity of 18 mS/cm and loaded onto a Heparin column (GE healthcare). The protein was eluted with a linear gradient of buffer B containing 1 M NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... Purification of the unprocessed form of cardosin B was performed by IMAC using a 1 mL HisTrap column (GE Healthcare, USA) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... followed by 2 CV of 100% IEX buffer B using an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare). To determine the point of elution of aSyn from the chromatography column protein fractions which were collected and monitored on absorption at 280 nm were ran on a 4-12% Bis-Tris gel (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellet was dissolved in 10 mL of buffer B and loaded onto a 1 mL HiTrap Heparin HP sepharose column (GE Healthcare) equilibrated with buffer B supplemented with 0.1 M NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... Conjugation of inhibitors to beads was performed by carbodiimide coupling to ECH Sepharose 4B (CTx-0294885, VI16832 and PP58) or EAH Sepharose 4B (purvalanol B) (GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... T cells were depleted by erythrocyte rosetting using sheep red blood cells and B cells were separated by Ficoll density gradient centrifugation as recommended by the manufacturer (GE Healthcare). The remaining erythrocytes were lysed in red blood cell lysis buffer (155 mM NH4Cl ...
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bioRxiv - Biochemistry 2021Quote: ... The RBD/RA of B-RAF (residues 150 -233) and RASSF5 (residues 199 - 367) were subcloned into pGEX-4T2 (GE Healthcare) to produce N-terminal GST fusion proteins ...
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bioRxiv - Biophysics 2023Quote: ... we repeated the dilution in Buffer B to 18 mS/cm conductivity and loaded it onto HiTrap SP columns (GE Healthcare). For elution ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM BME) with elution buffer B (buffer A with 500mM Imidazole) on a 5mL His-Trap FF Crude Nickel Column (GE Healthcare). Fractions containing Pol θ were pooled and were separated further on a 5mL HiTrap Heparin HP (GE Healthcare ...
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bioRxiv - Systems Biology 2024Quote: ... SP3 magnetic beads were prepared as follows: per 10 samples 20 µL of each Sera-Mag Speed Beads A and B (GE Healthcare) were mixed 1:1 ...
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bioRxiv - Microbiology 2023Quote: ... Peak fractions were collected and diluted with buffer B to a conductivity of 18 mS/cm and loaded onto HiTrap SP columns (GE healthcare). A linear gradient of buffer B containing 1 M NaCl was used for elution ...
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bioRxiv - Microbiology 2023Quote: ... The sample was additionally diluted with buffer B to a conductivity of 18 mS/cm and loaded onto a Heparin column (GE healthcare). The protein was eluted with a linear gradient of buffer B containing 1 M NaCl ...
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bioRxiv - Bioengineering 2024Quote: ... The LV myocardial wall was segmented from the LV B-mode series using speckle-tracking software to delineate the endocardium (EchoPac, version 214, GE Healthcare). The spatial and temporal resolutions of the acquired Doppler images (e.g. ...
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bioRxiv - Developmental Biology 2024Quote: ... The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3) and purified using glutathione Sepharose 4 B (GE Healthcare). An antibody against Ccrk was obtained by immunizing guinea pigs with purified GST-fused Ccrk.
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bioRxiv - Biophysics 2024Quote: ... we repeated the dilution in buffer B to 18 mS/cm conductivity and loaded it onto HiTrap SP columns (GE Healthcare). For elution ...
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bioRxiv - Cancer Biology 2022Quote: ... Live cells were enriched using Ficoll solution (GE Healthcare, 17-5446-02). For density gradient based enrichment of cells ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was applied to a Phenyl-sepharose HP (GE Healthcare) column equilibrated with 20 mM MES-NaOH pH 6.0 ...
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bioRxiv - Cell Biology 2020Quote: ... The solution was added to glutathione Sepharose 4B (GE Healthcare, Chicago, IL) and incubated 4 h at 4°C under agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein bands were detected using enhanced chemiluminescence (ECL) Select Solution (GE healthcare) and an ECL Pico System (ECL-PS100 ...
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bioRxiv - Neuroscience 2020Quote: ... IPG strips were rehydrated overnight with DeStreak™ Rehydration Solution (GE Healthcare) and 1% (v/v ...
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bioRxiv - Biophysics 2020Quote: ... we load the solution on a 1 mL MonoQ column (GE Healthcare) using an FPLC system (Äkta Explorer) ...
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bioRxiv - Biochemistry 2021Quote: ... and the filtered solution was loaded into a HisTrap column (GE Healthcare). The proteins were eluted with the imidazole gradient ...