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Citations for GE Life Sciences :
251 - 300 of 6498 citations for 6H Dibenzo a g quinolizinium 5 8 13 13a tetrahydro 2 9 dihydroxy 3 10 dimethoxy 7 methyl 7S 13aS since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Examinations were carried out with a linear array i13L probe (10-14 MHz) using a Vivid 7 Dimension ultrasound system (GE Healthcare Ultrasound, Horten, Norway). The operator was blinded to the genotype of the mice ...
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bioRxiv - Immunology 2021Quote: ... Reduced proteins were run on 8%,10% or 12% SDS-PAGE gels and transferred on to 0.22 μm nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked using 5% skim milk in 1X Tris-buffered saline and 0.1% Tween 20 (1XTBST ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples at concentrations >8 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... Radioactive signal was detected on a Typhoon 9 500 FLA scanner (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... Radioactive signal was detected on a Typhoon 9 500 FLA scanner (GE Healthcare). Quantification of the EtBr and radioactive signals was performed with Fiji software (80) ...
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bioRxiv - Biochemistry 2021Quote: ... flow rate 0.8 ml/min) or Superdex 200 Increase 5/150 (~3 ml, flow rate 0.45 ml/min) columns (GE Healthcare) were used ...
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bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was then centrifuged at 18,800 × g for 10 min and the resulting supernatant was applied to His Spin Trap (GE Healthcare). After centrifugation (100 × g ...
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bioRxiv - Biophysics 2021Quote: ... The dye-labeled protein was purified using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50000 rpm for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... for 30 minutes with or without cofactor GMPPNP (30 μM) then applying 0.5 mL to a PD-10 Sephadex G-25 gel-filtration column (GE Healthcare). Eluted fractions (0.5 mL ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane extract was centrifuged for 35 mins at 200,000 x g and the supernatant loaded onto a 10 ml Nickel column (Chelating Sepharose; GE Healthcare) equilibrated in TSB with 0.2% DDM pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated to 0.5 ml and after centrifugation at 21,700 g for 20 min directly applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) in SEC buffer (50 mM TRIS pH 7.8 ...
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bioRxiv - Neuroscience 2020Quote: Protein samples concentrated to ∼1.0 g/L were injected into an HPLC-driven SEC column (Superdex 200 10/30 column, GE Healthcare) equilibrated with HBS-C buffer ...
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bioRxiv - Biochemistry 2019Quote: ... The most concentrated IR-ECD-containing fraction was either desalted using disposable 8.3 ml Sephadex G-25 PD-10 desalting columns (GE Healthcare) equilibrated with HBS by centrifugation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The unreacted DBCO molecules were eliminated by a Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences). To make fluorescently labeled DBCO modified FBS proteins or individual proteins (BSA or APO H) ...
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bioRxiv - Plant Biology 2019Quote: ... The suspension was centrifuged for 15 minutes at 4°C and 15,000 g to remove cell debris and the clear supernatant was applied to a PD-10 desalting column (GE healthcare) to remove excess free biotin using the gravity protocol according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Labeled NBD1 was spun at 4 °C and 16,000 g for 30 minutes and passed through a gel filtration column (Superdex 200 Increase 10/300 GL - GE Healthcare) previously equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Extracts were cleared by centrifugation for 10 min at 21,000 × g at 4°C and incubated while rotating with 200 μl IgG Sepharose beads (GE Healthcare) for 4 hrs at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... with purification of labelled protein away from free label using a desalting Superdex G-25 (PD-10) column (GE Healthcare). FRET was examined using 495 excitation for FITC-HU-A ...
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bioRxiv - Microbiology 2021Quote: ... DTT was removed via two consecutive passes through a PD-10 Sephadex G-25 M buffer exchange column (GE Healthcare) as per the manufacturer’s instructions into labelling buffer (50 mM HEPES pH 7.1 ...
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bioRxiv - Biophysics 2022Quote: ... The solution was subjected to a 5 min spin at 17,000 x g in a refrigerated microcentrifuge before separation on a Superdex 200 10/300 column (GE Healthcare) equilibrated with SEC buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... the sample was clarified 400,000 x g for 30 minutes and applied to a Superdex 200 Increase 10/300 GL (GE Healthcare) gel filtration column equilibrated in G-buffer containing 0.2 M ammonium acetate ...
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bioRxiv - Biochemistry 2023Quote: ... and collected by centrifugation at 15,000 g for 10 min at 20 °C followed by size exclusion chromatography using a PD10 column (GE Healthcare). Importantly ...
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bioRxiv - Cell Biology 2024Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer and 0.150 M NaCl) using PD-10 desalting columns or Midi-Trap G-25 (GE Healthcare). The purity of the proteins was evaluated using SDS–PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Systems Biology 2021Quote: ... GTP-loaded protein was buffer exchanged into either NMR buffer or the GTPase assay buffer using NAP-5 Sephadex G-25 DNA Grade columns (GE Healthcare # 17085301). We were unable to obtain sufficient material for some mutants (H141E/I ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were obtained by centrifugation at 12,000 x g for 15 min and subjected to affinity purification on a 5 mL Excel HisTrap affinity purification column (GE Life Sciences) equilibrated with IMAC-A buffer (20 mM sodium phosphate buffer pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... for 10 min and blocked in PBS containing 2% bovine serum albumin (GE Healthcare) and 0.01% Tween 20 (National Diagnostics ...
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bioRxiv - Biophysics 2021Quote: ... The flow through containing SARS-CoV-2 Mpro was collected and further purified using size exclusion chromatography column (G-100, GE Healthcare,) equilibrated with 20 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... DksA was exchanged into a buffer containing 15 mM sodium phosphate and 100 mM NaCl (pH 7) over a PD-10 Sephadex column (GE Healthcare Bio-Sciences, Pittsburg, PA). DksA protein solutions (12.5 μM ...
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bioRxiv - Biochemistry 2022Quote: ... COS-7 cells were cultured in DMEM (GE Healthcare) supplemented with FCS (PAN-Biotech ...
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bioRxiv - Developmental Biology 2021Quote: Single cell suspension was collected by centrifugation (3 min at 300 g) and total RNA was extracted by Illustra™ RNAspin RNA Isolation Kit (GE Healthcare GE25-0500-72) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Membranes were washed with PBS-T for 3 × 10 min and were developed by ECL Prime (GE Healthcare) and imaged on a Biorad ChemiDoc MP Imaging System.
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 times for 5 min each with 1X TBST buffer and developed by chemiluminescence (GE healthcare, RPN2106). The Odyssey FC imaging system (LI-COR ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose (Cytiva #SH30243.02) supplemented with 10% fetal bovine serum (GE Life Sciences #SH30910.03), 2 mM L-Glutamine (GE Life Sciences #SH30852.01) ...
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bioRxiv - Biochemistry 2019Quote: ... dynein constructs were mixed with 20 μM Halo Alexa488 dye and incubated on ice for 10 min and a PD MiniTrap G-25 column (GE Healthcare) equilibrated with dynein gel filtration buffer was used to remove excess dye afterwards.
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bioRxiv - Biochemistry 2019Quote: ... centrifuged for 10 min at 5000 g and the lysate was passed through a Source-15Q anion exchange 55 ml column (GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Bioengineering 2021Quote: ... which exhibits a dissociation binding constant approaching attomolar concentrations (KD~10−18 M).[20] EDTA was added to formulations (4 eq with respect to zinc) to sequester zinc from the formulation and then lispro was isolated using PD MidiTrap G-10 gravity columns (GE Healthcare) to buffer exchange into water ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation at 4000 g for 10 min and then incubated with 1 ml (packed volume 600 μl) of lgG Sepharose beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... were added to the lysate (2 μg/ml cyclin B1 and 10 μg/ml cyclin A final concentration) together with protein G Sepharose (20 μl of 50% slurry bead volume; GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... Plexins and GTPases were further purified by either size exclusion chromatography or using a PD-10 Sephadex G-25 desalting column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Insoluble material was removed by ultracentrifugation at 150,000 x g for 20 min before purification by SEC on a Superpose 6 increase 10/300 GL column (GE Healthcare) equilibrated with buffer (25 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... and the excess of dye was removed by using a PD Minitrap G-25 column for NPM or a Superdex 200 10/300 GL (GE Healthcare) size exclusion chromatography column for SET/TAF-Iβ ...
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bioRxiv - Cell Biology 2022Quote: ... 500 µg of the purified protein or the complex was loaded on a Superdex 200 10/300 size exclusion column (GE Healthcare) in line with both a DAWN HELIOS II MALS detector (Wyatt Technology ...
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bioRxiv - Biochemistry 2022Quote: ... 3C protease was removed by Ni-chelated sepharose and the heterotrimetric G-protein was further purified with MonoQ 10/100 GL column (GE Healthcare). Protein was bound to the column and washed in buffer A (20 mM HEPES ...