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Citations for GE Life Sciences :
451 - 500 of 6498 citations for 6H Dibenzo a g quinolizinium 5 8 13 13a tetrahydro 2 9 dihydroxy 3 10 dimethoxy 7 methyl 7S 13aS since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Samples were spun to pellet insoluble fraction and loaded on a 13-cm Immobline Drystrip isoelectric-focusing gel (pH 3.0-10.0; GE Healthcare), and proteins were separated in the first dimension using the IPGphor isoelectric-focusing system (Amersham Pharmacia Biotech ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting supernatant was passed through either a 0.45 or 0.8 μm pore size filter and loaded onto a 5 mL Ni+2-NTA HisTrap HP column (GE Healthcare) pre-equilibrated with binding buffer (20 mM imidazole ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... Clear lysates were filtered through 0.45 μm and immediately applied at 2 mL/min onto a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The next day the protein was dialyzed for an additional 3 hours in fresh storage buffer and subsequently applied to a Superdex 200 10/300 GL (GE Life Sciences) equilibrated in the same buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The eluate fraction was dried using a vacuum centrifuge and resuspended in 3.6 mL ampholyte solution (pI 3-10, GE Healthcare, Chicago, IL). Offgel fractionation was performed using a 3100 OFFGEL fractionator (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... the NbaAgo samples (3 mg/ml in 33 μl) were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare, USA) operated on a ProStar335 system and connected to a tandem of Prostar 335 detector (Varian ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... and loaded on a equilibrated G-25 column (Column PD Minitrap G-25, GE Healthcare, Cat.# 28-9180-07). Once the solution fully entered the column ...
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bioRxiv - Molecular Biology 2023Quote: ... The signals of the transferred radioactively labelled methyl groups were detected by autoradiography using a Hyperfilm™ high performance autoradiography film (GE Healthcare) at −80 °C in the dark ...
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bioRxiv - Plant Biology 2020Quote: ... [8-3H]-hypoxanthine (925 GBq/mmol; GE Healthcare UK Limited); trans-[2-3H]-zeatin (592 GBq/mmol ...
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bioRxiv - Cell Biology 2020Quote: siRNA-mediated knockdown in 786-O cells was carried out using 5-10 nM Dharmacon ON-TARGETplus siRNAs (GE Healthcare), diluted in siRNA buffer and mixed with DharmaFECT transfection reagent (1:50 in optiMEM reduced serum media) ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 μg proteins were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). To visualize MLKL oligomers ...
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bioRxiv - Microbiology 2019Quote: ... fumigatus pksP mutant conidia with an MOI of 10 or 5 in HBSS with Ca2+ and Mg2+ (HyClone, GE Healthcare) on a linear shaker (100 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... to 1/10 of the starting volume and loaded on a 5 ml Ni2+ HiTrap IMAC HP column (GE Healthcare). A linear 0-75 mM histidine gradient with elution buffer was used to elute proMbxA ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatant was filtered using 0.2 μm filters and buffer exchanged in storage buffer (20 mM Tris-HCl pH7.4, 200 mM NaCl and 5% glycerol) using PD-10 desalting columns (GE healthcare). The desalted sample then was aliquoted and stored at -80°C until the one-step purification/ immobilisation.
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bioRxiv - Biochemistry 2021Quote: ... 5 mL imidazol gradient) or by size exclusion chromatography (SEC) using a Superdex® 75 10/300 GL (GE Healthcare) with a flow rate of 0.5 mL/min and dialysis buffer as running buffer ...
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bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 mM dithiothreitol (DTT), and concentrated using Vivaspin 5 kDa (HipBLp, HipSLp) and 10 kDa (HipTLp) cutoff columns (GE Healthcare). Protein concentration was quantified using the Bradford assay according to the manufacturer’s instructions (BioShop) ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was then dialyzed against 10 mM Tris HCl pH 8.0 and loaded on MonoQ 5/15 column (GE Healthcare) to be eluted using a step gradient of 240 mM and 400 mM NaCl in the same buffer.
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bioRxiv - Bioengineering 2023Quote: ... 13 fetuses at 23w to 37w GA scanned on a 1.5 T GE scanner (Signa HDxt, GE Healthcare, United States) were used for the following analysis ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) containing 5% skim milk (Meiji) and subjected to primary antibody for 2 h and HRP-conjugated secondary antibody (GE Healthcare) for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... Peak fractions were pooled, diluted ~5 fold with buffer 0 (20 mM HEPES, 2 mM DTT) and loaded onto a Q column (GE Healthcare). Rad33 was then eluted from the Q column using a linear gradient of Q buffer A (20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots were taken every 30 minutes for a period of 2 hours and quenched in 3ml of 5% trichloroacetic acid (TCA) on Whatman-glass fiber filters (GE healthcare) and washed 3 times with 1ml ethanol to dry the filter ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... diluted by half to lower NaCl concentration to 250 mM and applied at 2 mL/min onto a HiTrap Heparin HP 5 mL column (GE Healthcare) previously equilibrated in Tris 50 mM ...
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bioRxiv - Cell Biology 2019Quote: ... the primary antibody was visualized using 2~5 μg/ml secondary antibody towards specific species of primary antibody labeled with Cy3B (GE Healthcare) or Atto 488 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Samples (100 µL OXAVL02 or OXAVL06 at 0.5-1 mg/mL) were loaded onto a Superdex® 200 Increase 10/300 G column (GE Healthcare Bio-Sciences AB Uppsala, SW) pre-equilibrated with the “SECMALS-PBS buffer” ...
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bioRxiv - Biochemistry 2020Quote: ... The redissolved protein sample was desalted using a NAP10 desalting column pre-packed with Sephadex G-25 resin (NAP™-10 Columns, Sephadex™ G25, GE Healthcare Life Sciences, UK) and eluted in 20 mM sodium phosphate buffer (pH 6.0) ...
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bioRxiv - Microbiology 2021Quote: ... These were stored in a chilled compartment at 9°C within a skirted 384 well plate (GE Healthcare) before being washed over the chip surface in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 2 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of media was concentrated over 2 ml heparin affinity medium (Heparin Sepharose 6 Fast Flow, GE Healthcare) packed into a 10 ml Poly-Prep Chromatography gravity flow column (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... IEF was performed in 24 cm 4–7 NL Immobiline DryStrip (GE Healthcare) in an IPGphor unit (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and COS-7 cells were grown in high-glucose DMEM (GE Healthcare) supplemented with 10% bovine calf serum (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... Cardiac function was measured by Vivid 7 ultrasound machine (GE Healthcare, Pittsburgh, PA) and 13-MHz linear array transducer ...
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MK2 deficiency decreases mortality during the inflammatory phase after myocardial infarction in micebioRxiv - Physiology 2023Quote: ... Images were acquired using a Vivid 7 Dimension system (GE Healthcare, Horten, Norway) and a i13L probe (10-14 MHz ...
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bioRxiv - Cell Biology 2020Quote: ... the rat cardiomyocytes were purified using a Percoll gradient (0.458 g/ml to 0.720 g/ml; GE Healthcare, Chicago, USA). Cocultures with cdMiPs were performed in a 10:1 ratio (cdMiPs:cardiomyocytes ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Peroxidase-linked anti-mouse immunoglobulin G and peroxidase-linked anti-rabbit immunoglobulin G (both GE Healthcare Bio-Sciences, Pittsburgh, PA). Alexa Fluor 594-conjugated Donkey Anti-Mouse IgG (1:250 ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample (at a concentration of 3 mg·ml−1) were loaded onto either a Superdex 75 or 200 HR 10/300 GL columns (GE Healthcare, Madison, WI, USA), equilibrated in 25 mM Hepes (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were isolated and used for the separation in the first dimension with Immobiline Dry Stripes (pH range 3-10 NL, 18 cm, GE Healthcare, Uppsala, Sweden) [20] ...
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bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... 45 μl Protein G Sepharose beads (GE Healthcare) were added ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein G Sepharose beads CL-4B (GE Healthcare) were pre-equilibrated with lysis buffer ...
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bioRxiv - Genetics 2021Quote: ... GammaBind G Sepharose beads (GE Healthcare Life Sciences) were pre-washed and blocked in binding buffer and 0.1 mg/ml BSA ...
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bioRxiv - Biophysics 2021Quote: ... A PD MiniTrap G-25 column (GE Healthcare) was used to remove imidazole ...
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bioRxiv - Plant Biology 2020Quote: ... 50 □L of Protein G Sepharose (GE Healthcare) was used in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Protein G–Sepharose (GE Healthcare, 17-0618-01). Dynabeads Protein G (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... purified through MicroSpin G-25 Columns (GE Healthcare), and were used as probes ...