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Citations for GE Life Sciences :
151 - 200 of 6498 citations for 6H Dibenzo a g quinolizinium 5 8 13 13a tetrahydro 2 9 dihydroxy 3 10 dimethoxy 7 methyl 7S 13aS since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Germany) and differentiated into macrophages by culture for 7 days in RPMI1640 medium supplemented with 10% fetal bovine serum (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... 50 mM Bis-Tris pH 7) and further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare) and eluted against buffer D (50 mM Bis-Tris pH 7) ...
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bioRxiv - Biochemistry 2023Quote: ... Purification of His-tagged domains was achieved by nickel-affinity using purification buffer supplemented with 1 mM DTT and gel filtration chromatography in 50mM ammonium bicarbonate pH 7 using a Superdex 200 Increase 10/300 column (GE Healthcare) following published protocols67 ...
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bioRxiv - Biochemistry 2020Quote: ... The 100-µl sample (8 mg/ml) was loaded on a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with filtered and degassed SEC buffer containing 3% glycerol and 5 mM dithiothreitol instead of MgCl2 and β-mercaptoethanol ...
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bioRxiv - Biophysics 2021Quote: ... 0.5 M NaCl pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Biophysics 2022Quote: ... 180 µL of approximately 8 µM sample was injected onto a Superose6 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM TrisHCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Following centrifugation at 18000 ×g the soluble fraction was applied to a 5 ml HisTrap HP column (GE Healthcare). The column was washed and protein eluted along a gradient using 20 mM Tris pH8 ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for isoelectric focusing of samples loaded on 7 cm pH 4-7 Immobiline DryStrips (GE Healthcare, Uppsala, Sweden). We rehydrated each strip for 48 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... ectodomains of ZmFERL7/9 were cloned into pGEX-6P1 (GE Healthcare) with a N-terminal GST tag ...
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bioRxiv - Biochemistry 2022Quote: ... and 3 mM DTT and was loaded onto a MonoS 10/100 column (GE Healthcare). The protein was eluted with an increasing salt (KCl ...
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bioRxiv - Physiology 2019Quote: ... The sample was then passed through a 5 ml PD-10 (GE Healthcare) desalting column equilibrated with 10 mM Hepes ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM DTT and 5-10 ml of Q Sepharose resin (GE Healthcare) was mixed to capture PGL-3 protein molecules ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM DTT and 5-10 ml of Q Sepharose resin (GE Healthcare) was mixed to capture PGL-3 protein molecules ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM DTT and 5-10 ml of Q Sepharose resin (GE Healthcare) was mixed to capture PGL-3 protein molecules ...
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bioRxiv - Cell Biology 2023Quote: Transthoracic echocardiography was performed using a 10-14-MHz i13 probe and the Vivid 7 Ultrasound machine (GE Healthcare Ultrasound, Horten, Norway). Mice were lightly anesthetized with isoflurane ...
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bioRxiv - Immunology 2022Quote: ... 2 mM CaCl2 using a HiPrep 26/10 Desalting column (GE Healthcare) and subsequently treated with enterokinase overnight at room temperature to remove the strep-tag ...
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purification column was prepared using 2-3 mL IMAC Sepharose 6 Fast Flow beads (GE Healthcare) and charged with 0.1 N Nickel sulphate solution ...
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bioRxiv - Biophysics 2019Quote: ... This mixture was then filtered through a 0.2 μM filter and concentrated on a 10 kDa (for EC1-5/EC3-5 proteins) or a 100 kDa (for full length proteins) MWCO column (Vivaspin, GE Healthcare). The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Peak TopBP1b6-8 fractions (eluted with 50mM and 75mM imidazole) were desalted (HiTrap™ or HiPrep 26/10, GE Healthcare) in physiological buffer (10mM Na2H/NaH2 PO4 pH 7.6 ...
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bioRxiv - Genomics 2020Quote: ... Equivalent amounts of protein were resolved by 8-10% SDS-PAGE and blotted onto nitrocellulose (0.2 µm, Amersham Protran) with a semi-dry blotter (GE Healthcare). The membrane was stained with Ponceau S solution (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... 0.15 M (NH4)2SO4 pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Cancer Biology 2019Quote: ... and eluted material exchanged into PBS with Sephadex G-25 in PD-10 Desalting Columns (GE Life Sciences).
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bioRxiv - Pathology 2020Quote: ... free TPEN and free Zn were further removed by gel filtration on PD MidiTrap G-10 (GE Healthcare) equilibrated with 40 mM of the metal-free HEPES-KOH ...
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bioRxiv - Microbiology 2020Quote: ... The assembly mix was concentrated at 350 × g using Vivaspin filters (10 kDa molecular weight cutoff; GE Healthcare) and subjected to another round of size-exclusion chromatography using the same column and buffer to remove unassembled components ...
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bioRxiv - Microbiology 2022Quote: ... Buffer exchange was performed using 5E10 VPs and PD-10 MiniTrap G-25 columns (GE Healthcare, Solingen, Germany) following the companies instructions with the “gravity” protocol ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and the buffer was exchanged with ultrapure water ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and buffer was exchanged for ultrapure water ...
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bioRxiv - Systems Biology 2023Quote: ... followed by DTT removal using a PD-10 desalting column with Sephadex G-25 resin (from GE healthcare). The reduced protein was mixed with a ten fold excess of the nitroxide spin label MTSL and incubated at room temperature protected from light for one hour ...
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bioRxiv - Microbiology 2020Quote: ... The IgG anti-SARS-CoV-2 was purified from plasma of a convalescent patient using a 5/5 HiTrap rProtein-A column (GE Healthcare, USA). See detailed information about this procedure in Supplementary method No.1.
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bioRxiv - Cell Biology 2021Quote: ... Postnuclear supernatants were incubated with 3 μg of antibodies coupled to protein A– or protein G–Sepharose beads (GE Healthcare, Solingen, Germany) for 4 h at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
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bioRxiv - Plant Biology 2023Quote: ... 5-mM 2-mercaptoethanol) before being applied to a HiTrap Heparin HP (GE Healthcare), and eluted by a linear NaCl gradient up to 1.0 M ...
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bioRxiv - Biophysics 2019Quote: ... The bacterial lysate was clarified using centrifugation at 20,000 x g for 40 min and loaded onto a 5 ml His-Trap column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... the lysate spun down at 12,000 x g for 30 min and then loaded onto a 5 ml His-Trap Crude FF column (GE Healthcare) using a ÄKTA Purifier protein purification system (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysate was centrifuged at 38,000 ×g and the supernatant was loaded onto a 5 mL HisTrap Ni-NTA column (GE Healthcare). The column was washed with HisTrap Buffer and protein eluted with a linear gradient of imidazole from 25 to 300 mM in HisTrap Buffer over 10 column volumes.
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bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation for 1 hour at 38,700 x g and passed over a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 15 column volumes of 20 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2022Quote: ... The sample was exchanged into the D2O buffer using a NAP-5 Sephadex G-25 column (GE Healthcare, Chicago, IL). For dUMP and TMP (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was cleared by centrifugation at 100,000 x g for 45 min and loaded onto a 5 mL HisTrap HP column (GE Healthcare). The column was washed extensively with NiA buffer ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins were collected after a 5 min centrifuge at 17,000 g at 4 ℃ and immobilised on Glutathione-coated sepharose beads (GE Healthcare). For pull down with 35S-radiolabelled preoteins ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...