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Citations for GE Life Sciences :
3001 - 3050 of 4416 citations for Dengue Virus Serotype 3 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... thawed protein samples were purified by size-exclusion chromatography using a Superdex 200 (16/600) column (GE Healthcare) in equilibrated in buffer 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2023Quote: ... The ErCas12a protein in clarified lysate was sequentially purified using immobilized metal affinity chromatography (HisTrap HP, GE Healthcare) and heparin chromatography (HiTrap Heparin HP ...
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bioRxiv - Biochemistry 2023Quote: ... 200 µL of protein for analytical runs) was injected into the Superdex 75 size exclusion column (GE Healthcare) (volume less than 900 µL) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified over a size exclusion chromatography column (Hiload 16/60 Superdex75 column, GE Healthcare) equilibrated with buffer containing 20 mM MES pH 6.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 10 mM glutathione and further purified on a Superdex 200 10/300 (GE Healthcare) column in 50 mM Hepes pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was then loaded onto a HiLoad Superdex 75 16/60 size exclusion chromatography column (GE Healthcare) that had been pre-equilibrated in Hepes 30 mM ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified using a 20 mL NiNTA column (Cytiva) attached to an Akta M FPLC (GE Healthcare) and with a 300 mM imidazole elution buffer ...
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bioRxiv - Microbiology 2023Quote: ... The immunocomplex was captured by using protein G Sepharose 4 fast flow beads (17-0618-01/ GE Healthcare). The immunoprecipitated complex was washed two times with IP lysis buffer and one time with mili-Q ...
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bioRxiv - Biochemistry 2023Quote: ... The GST fusion protein was purified by affinity chromatography on glutathione-Sepharose (GE Healthcare Life Sciences (now Cytiva)) in 300 mM NaCl ...
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Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulationbioRxiv - Biochemistry 2023Quote: ... The spin-labeled proteins were further purified by HiLoad 26/600 Superdex 200 pg column (GE life sciences) in a buffer of 150mM NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... Protein containing fractions were pooled and buffer exchange was carried out using a PD-10 column (GE Healthcare) using TES-KCl buffer (25 mM TES/KOH pH 7.5 ...
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bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS/PAGE in 10% gel before wet transfer to 0.45μm nitrocellulose membranes (GE Healthcare) and blocked in 5% dry milk powder in TBS-T (100mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... then stored at −20°C before purification by fast protein liquid chromatography (FPLC) (AKTA Purifier; GE Healthcare, UK) using a pre-packed HiLoad Superdex 30 26/600 column equipped with a UV detector ...
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bioRxiv - Biochemistry 2023Quote: ... Aurora A protein was thawed and buffer exchanged in the experimental buffer using NAP-5 Columns (GE Healthcare). Experiments typically involved titrating 25 µM of protein in the sample cell with 300 µM of compound in the syringe ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was further purified by size exclusion chromatography (SEC) (Superdex 200 Increase 10/300 GL, GE Healthcare) in 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing purified proteins were concentrated and further purified by a Superdex 200 size-exclusion column (GE Healthcare) equilibrated in buffer containing in the 25 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: The protein was further purified by size exclusion chromatography (SEC) (Superdex 200 Increase 10/300 GL, GE Healthcare) in 50 mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2023Quote: ... S17 were prepared by blotting tau and α-synuclein proteins onto nitrocellulose membrane (GE Healthcare Life Science, 10600004). Human HT40 fibrils (Stressmarq ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrated protein sample was loaded on a pre-equilibrated Superdex-75 16/60 column (GE Healthcare, USA). A buffer consisting of 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2023Quote: ... the target protein was further purified using a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare). The size-exclusion column buffer contained 50 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a nitrocellulose membrane with a 0.2 μm pore size (GE Healthcare Life Sciences, 10600001) by wet transfer at 100 V for 120 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein eluate was then applied to an ion exchange chromatography column (2x 1ml HiTrap Q HP, GE Healthcare) equilibrated with QA buffer ...
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bioRxiv - Immunology 2024Quote: ... Proteins were separated on 12% SDS polyacrylamide gels and transferred to nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membranes were blocked in 5% dried milk/TBS-T buffer and incubated over night with anti-mouse TREX1 antibody (clone C-11 ...
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bioRxiv - Biochemistry 2024Quote: ... and the flowthrough containing phosphorylated proteins was purified over Hiload 16/600 Superdex 75 pg column (GE Healthcare) preequilibrated with buffer containing 25 mM Tris (pH 7.5) ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing target protein were pooled and incubated over night at 4°C with PreScission protease (GE Healthcare) at a ratio of 1:100 (w/w ...
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bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... the protease was removed from (un)cleaved target protein using a HisTrap FF column (GE Healthcare Life Sciences). Remaining nucleic acids were removed using anion exchange chromatography (AIEX ...
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bioRxiv - Biophysics 2024Quote: ... Protein was concentrated and inject into size-exclusion chromatography on a Superdex 16/60 75 column (GE Healthcare) pre-equilibrated in buffer 20 mM Bis-Tris pH 7.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein bands were visualized and imaged by using an ImageQuant LAS 500 scanner (GE Healthcare, Chicago, IL, USA). After transfer ...
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bioRxiv - Microbiology 2024Quote: ... thawed protein samples were purified by size-exclusion chromatography using a Superdex 200 (16/600) column (GE Healthcare) in equilibrated in a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: Proteins were purified by immobilized metal-affinity chromatography (IMAC) on a HisTrap HP nickel-Sepharose column (GE Healthcare) at 4°C following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Elution fractions containing the most protein were combined and loaded directly onto a size-exclusion column (GE Healthcare) pre-equilibrated with lysis buffer without prior concentration or dialysis of the protein ...
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bioRxiv - Microbiology 2024Quote: ... All protein lysates were separated on 10% polyacrylamide gels and blotted on polyvinyl difluoride membranes (PVDF, GE Healthcare). Blotted membranes were blocked with either 5% non-fat milk or 5% BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein solutions was loaded on to Superose 6 Increase 10/300 GL column (GE Healthcare, Madison, WI) that was equilibrated with a buffer containing 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Immunology 2024Quote: ... The concentrated protein was injected into a Superdex 200 Increase 10/300 GL (GE Healthcare 28-9909-44) size exclusion column chromatography system (Waters) ...
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bioRxiv - Microbiology 2021Quote: ... albicans Phr1 and Pga4 gene without the sequence encoding signal peptide and the GPI anchor were constructed using primers listed in Supplementary Table 3 and cloned into the bacterial expression vector pGEX6P1 (GE healthcare, Stockholm, Sweden). Plasmids were then transformed into E ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample (at a concentration of 3 mg·ml−1) were loaded onto either a Superdex 75 or 200 HR 10/300 GL columns (GE Healthcare, Madison, WI, USA), equilibrated in 25 mM Hepes (pH 7.8) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and mixed with 20 μl of home-made SPRI beads (equivalent of 1 ml of Sera-Mag Magnetic SpeedBeads, carboxylated, 1 μm, 3 EDAC/PA5 (GE Healthcare Life Sciences) in 50 ml of binding buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... SepFΔML,K125E/F131A were covalently immobilized on 3 independent flow-cells of an NTA sensorchips as previously described 64 (GE Healthcare Life Sciences). The final immobilization densities of SepF variants ranged from 3000 to 4000 resonance units (RU ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were isolated and used for the separation in the first dimension with Immobiline Dry Stripes (pH range 3-10 NL, 18 cm, GE Healthcare, Uppsala, Sweden) [20] ...
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bioRxiv - Biochemistry 2020Quote: ... The fractions were collected and concentrated to a final concentration of 700 µM using Vivaspin sample concentrators (GE Healthcare; cut-off: 3 kDa). The protein purity was analysed by SDS-PAGE using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was incubated for 4 hours at 4°C using a rotating wheel with 3 mL of a GST bead slurry (GE Healthcare Life Sciences) pre-washed 3x with wash buffer (WB ...
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bioRxiv - Immunology 2024Quote: ... 1:3 blood dilutions were made with sterile Roswell Park Memorial Institute (RPMI) 1640 medium (HyClone; GE Healthcare Life Sciences, Chicago, IL, USA) supplemented with 100 unit/mL penicillin and 100 mg/mL streptomycin (Gibco Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: All experiments were performed on a 3 Tesla whole-body GE Premier system equipped with a 21-channel receive head coil (GE Healthcare, Milwaukee, WI). We used fluoroptic probes for the gold standard temperature measurement with a precision of 0.1 °C and a temporal resolution of 1 second (STB Medical ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... nitrocellulose membranes were blocked in 3 % BSA in TBST and stained with HRP-conjugated streptavidin (GE Healthcare Life Science, Cat# RPN1231vs,1:2500) in blocking solution ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Positive RDT samples were then used to create dried blood spots (DBS) on 3 mm thick Whatman® filter paper (Whatman, GE Healthcare, UK). The DBS was dried at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of purified DNA was labelled for 3 h at 15°C with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science).The templates used in this study are ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mm × 1 mm × 1 mm) and brain FDG-PET were acquired simultaneously on a hybrid 3 T SIGNA PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the CUB Hôpital Erasme (Brussels ...