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Citations for GE Life Sciences :
2901 - 2950 of 3622 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... the membranes were washed three times in TBST and incubated for 5 minutes with a chemiluminescent reagent (GE Healthcare) to visualize immuno-reactive bands ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through was further purified by cation-exchange chromatography on a 5 mL HiTrap S column (GE Healthcare) with a salt gradient of 0 to 1 M NaCl in 1×PBS supplemented with 1 mM DTT ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were exposed to X-ray for 5-10 min using a biomolecular imager (ImageQuant LAS4000, GE Healthcare, USA). Relative cytokine content was determined after quantification of the dot intensity using ImageJ.
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bioRxiv - Biochemistry 2021Quote: ... and those containing GsI-IIC RT were pooled and loaded onto a 5-ml Heparin HP column (GE Healthcare) pre-equilibrated in Heparin Buffer A (20 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was filtered (0.45 µm) and then loaded onto a 5 ml HisTrap FF Crude column (GE Healthcare) equilibrated with wash buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... All proteins were purified by Ni-NTA affinity chromatography using a 5 ml HisTrap column (GE Healthcare Life Sciences) equilibrated with 50 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Following centrifugation at 18000 ×g the soluble fraction was applied to a 5 ml HisTrap HP column (GE Healthcare). The column was washed and protein eluted along a gradient using 20 mM Tris pH8 ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of complement component C5 at 31.6 μM (5.96 mg/ml) was injected onto a Superdex 200 Increase 5/150 column (GE Healthcare) at a flow rate of 0.35 ml/min ...
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bioRxiv - Molecular Biology 2021Quote: ... coli BL21 SI pRARE at 18 °C overnight and purified using a HisTrap FF 5-ml column (GE Healthcare) in standard buffers (50 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... the histones were purified from inclusion bodies under denaturing conditions on a 5 mL HiTrap SP FF (GE Healthcare) cation exchange column on a Next Generation Chromatograph (NGC ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Plant Biology 2022Quote: ... The filtrate was applied to a 5 mL HisTrap HP Nickel Sepharose™ affinity column (GE Healthcare Life Sciences) and washed with five column volumes of binding buffer (50 mM sodium phosphate buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Dialyzed protein was then loaded onto a 5 ml HiTrap Q HP anion exchange column (GE Healthcare Life Sciences) and eluted with a gradient of 0-100% elution buffer (50 mM Tris-HCl (pH 8) ...
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bioRxiv - Plant Biology 2022Quote: ... The dialyzed samples were applied to a 5 ml HiTrap Q HP anion exchange column (GE Healthcare Life Sciences). The Q anion exchange column was eluted via linear gradients from 0% to 100% elution buffer (50 mM Tris ...
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bioRxiv - Synthetic Biology 2022Quote: ... The soluble fraction was loaded onto a 5 mL Ni-NTA column connected to an ÄKTA pure (GE Healthcare) chromatography system ...
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bioRxiv - Neuroscience 2022Quote: ... The column was washed with 10 column volumes of Buffer A (10 mM HEPES, pH 7.3, 0.15 M NaCl, 5 mM imidazole) on an Aktapurifier (GE Healthcare) while collecting fractions ...
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bioRxiv - Microbiology 2024Quote: ... 30 min) and loaded onto a 5 ml HighTrap MBP column (Cytiva) using an Äkta-Pure system (GE Healthcare). The column was washed with 10 column volumes of lysis buffer and protein eluted with MalE-elution buffer (100 mM Tris-HCl pH7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... supernatant filtered through 0.45 µm syringe filter and loaded onto a HiTrap Q HP column (5 ml, GE Healthcare) that was stacked on top of a SP column (5 ml ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered (0.45µm) and loaded onto a Ni2+-affinity column (HisTrap FF, GE Healthcare 5 mL column) equilibrated in low imidazole buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins in the soluble fraction were purified by Ni-affinity column chromatography (HisTrap 5 ml column, GE Healthcare). The His6-tag was removed by thrombin digestion (1 μg of thrombin per mg of TelSi3 protein ...
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bioRxiv - Bioengineering 2023Quote: ... The flowthrough sample was collected and loaded onto the pre-equilibrated HiTrap SP HP 5 mL column (GE Healthcare) with a flow rate of 2.5 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lysate was then 0.45 μm filtered and applied to a 5 mL HisTrap HP column (GE Healthcare). The column was washed with five column volumes of wash buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Biochemistry 2023Quote: Dimer formation of non-farnesylated GBP1 was analyzed by analytical size-exclusion chromatography (Superdex 200 5/150, GE Healthcare) as described (Ince et al ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µM Drp1 was injected onto a Superose 6 10/300GL column in an ÄKTApure FPLC system (GE Healthcare) connected in line with DAWN Heleos-II 18-angle MALS and Optilab T-rEX differential refractive index (dRI ...
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bioRxiv - Cell Biology 2023Quote: ... PARP14 MD1 was additionally purified by ion exchange chromatography using a HiTrap™ 5 mL SP HP (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were exposed to X-ray for 5-10 min using a biomolecular imager (ImageQuant LAS4000, GE Healthcare, USA). Relative cytokine content was determined after quantification of the dot intensity using ImageJ.
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bioRxiv - Biophysics 2023Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap CaptoS 5 ml column (GE Healthcare, USA) for cation exchange ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed 5 more times and the signal was detected by chemiluminescence using ECL Prime (GE Healthcare) on an Amersham Imager 600 detection system (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE using 4% or 5% polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with mouse monoclonal antibody generated against HA-tag peptide (YPYDVPDYA ...
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bioRxiv - Biophysics 2023Quote: ... Data collection was carried out at 20 °C using a Superdex200 Increase 5/150 analytical SEC column (GE Healthcare) equilibrated in the appropriate buffers (see Table S3 ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected after 6 days and purified using a 5-ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5-ml kappa-select column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was subjected to Ni-immobilized metal affinity chromatography (IMAC) (HisTrap FF Crude column, 5 ml, GE Healthcare). Proteins were eluted with a linear gradient of imidazole (10-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... Sortase ligated PZA was purified in a single step over a 5-ml SP anion exchange column (GE Healthcare) to minimize protein degradation ...
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bioRxiv - Microbiology 2024Quote: ... 5 ml of pooled mouse sera was then used to purify IgG using Protein A Sepharose resin (GE Healthcare) and digested for using papain-agarose resin (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... purified I53-50B.4PT1 was immobilized on Ni2+-NTA resin in a 5 mL HisTrap HP column (GE Healthcare) equilibrated with the following buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Commercial human fibrinogen (America Diagnostica Inc) was desalted on a 5-ml HiTrap desalting column (GE Healthcare, IL, USA), eluted at a constant flow rate (1 ml/min ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was supplemented with 10 mM imidazole and loaded onto a 5 ml Ni-NTA column (GE Healthcare). Beads were first washed with HBS containing 0.01% (w/v ...
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bioRxiv - Bioengineering 2024Quote: ... A second protein purification step was performed using a cation-exchange column (HiTrap SP HP, 5 ml, GE Healthcare) with a high salt elution buffer (50 mM Tris–HCl at pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were collected after 6 days and purified using a 5-ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5-ml kappa-select column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was filtered through 0.8 µm membranes and applied to a HisTrap HP 5 mL column (GE Healthcare) pre-equilibrated with Buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
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bioRxiv - Neuroscience 2021Quote: Whole-brain images will be acquired at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison HealthEmotions Research Institute (Department of Psychiatry) using a 3 Tesla GE MR scanner (Discovery MR750; GE Healthcare, Chicago, IL, USA). A high-resolution T1 image will be acquired with a fast spoiled gradient recalled echo sequence (8.2 ms TR ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in 10 ml FACS wash and filtered through a nylon filter before being overlayed onto 3 ml Ficoll (GE Healthcare, 17-1440-03). Cell/Ficoll mixture was centrifuged at 931g for 1 hour at room temperature with brakes off ...
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bioRxiv - Plant Biology 2022Quote: ... 3 mL of the centrifuged supernatant was applied to a PD-10 column filled with Sephadex G-25 resin (GE Healthcare, Chicago, IL, USA) equilibrated with the homogenizing buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed with TBS containing 0.1% Tween 20 (3 times for 15 min under shaking at RT) and probed with an anti-rabbit HRP conjugated antibody (NA934V, GE healthcare, Piscataway, NJ, USA) for 2 h at RT ...
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bioRxiv - Immunology 2024Quote: ... The cultures were started in a volume of 400 mL of basal medium at a cell density of 350,000-400,000 cells/mL and were supplemented with Cell Boost 7a (Hyclone) at 3% v/v and Cell Boost 7b (Hyclone, GE Healthcare, Chicago, IL, USA) at 0.3% v/v on days 0 ...
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bioRxiv - Genetics 2021Quote: ... DNA was transferred in 2 x SSC to positively charged nylon membranes (GE Healthcare Amersham Hybond-N+) and was then immobilized by ultraviolet cross-linking (1200 J) ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 μL lysate (2 mg protein) was injected through a 0.75mm loop (GE Healthcare 18-1112-53). Run parameters were 0.5 mL/min and 1.5MPa for 1 column volume ...