Labshake search
Citations for GE Life Sciences :
2801 - 2850 of 3622 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... the supernatant containing complexes of Panx and LC8 was loaded onto 5 ml HisTrap Fastflow column (GE Healthcare). After extensive washing with Binding buffer ...
-
bioRxiv - Pathology 2021Quote: ... diluted five-fold with extraction buffer and applied to a 5 ml HisTrap column (GE Healthcare, Chicago, IL) equilibrated with extraction buffer ...
-
bioRxiv - Biophysics 2020Quote: ... The EcA2 used for these experiments were previously desalted in a 5-mL HiTrap Desalting (GE Healthcare, USA) and eluted in 10 mM sodium phosphate at pH 7.0 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The lysate obtained was loaded onto the pre-equilibrated nickel affinity column (HisTrap FF, 5 mL, GE Healthcare) with loading buffer (50 mM KPi ...
-
bioRxiv - Biophysics 2020Quote: ... Fractions containing Pol I were then loaded onto a 5-mM HiTrap Heparin HP column (GE Life Sciences) equilibrated in Heparin BufferA(50mMTris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: Approximately 5 μg of GST or GST-tagged protein fragments were incubated in Glutathione-Sepharose beads (GE Healthcare) in GST-lysis buffer at 4°C overnight ...
-
bioRxiv - Microbiology 2023Quote: ... proteins were separated by immobilized-metal affinity chromatography (IMAC) on a 5 mL HisTrap HP column (GE Healthcare), previously washed with ddH2O and equilibrated with buffer B (25 mM HEPES/NaOH pH 7.5 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Chemiluminescence was detected after 5 min of incubation with ECL™ Select Western Blotting Detection Reagent (GE Healthcare) in a ChemiDoc chemiluminescence detector (BioRad) ...
-
bioRxiv - Biochemistry 2024Quote: ... The Spt4/5 heterodimer was further purified by size exclusion chromatography (Superdex 75 10/300 GL, GE Healthcare) through isocratic elution in low salt buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... We then applied the supernatant onto two tandem 5 mL HiTrap Q HP anion exchange columns (GE Healthcare) pre-equilibrated with 20 mM Tris pH 8.0 ...
-
bioRxiv - Immunology 2024Quote: ... blots were incubated at room temperature for 5 min in the dark with ECL detection reagent (GE Healthcare), made up in accordance with manufacturers’ instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Then the solution was injected onto a prewashed SP Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL). The column was washed with 20 CV of Buffer D (20 mM HEPES-KOH pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Aurora A protein was thawed and buffer exchanged in the experimental buffer using NAP-5 Columns (GE Healthcare). Experiments typically involved titrating 25 µM of protein in the sample cell with 300 µM of compound in the syringe ...
-
bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated hMDH preps were loaded onto a 5 mL HiTrap Butyl HP column (GE Healthcare). An inverse linear gradient of ammonium sulfate from 1.5 M to 0 M was used to purify hMDH where it eluted at a conductivity of 135 mS/cm ...
-
bioRxiv - Biophysics 2023Quote: ... centrifuged and applied to a cation exchange chromatography column (5 mL HiTrap SP HP, GE Healthcare Life Science) equilibrated with 25 mM sodium phosphate ...
-
bioRxiv - Biochemistry 2023Quote: ... a second nickel-affinity chromatography was performed (His Trap, 5 mL, GE Healthcare; equilibrated in 10% HisB buffer). Sample was loaded and unbound protein further washed out with 10% HisB ...
-
bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... Recombinant Sau-MenD was purified at room temperature by IMAC with 5 mL HisTrap HP columns (GE Healthcare) and an imidazole gradient of 20–500 mM over 50 mL ...
-
bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was added to a 5 mL Ni-Sepharose fast-flow resin (GE Healthcare Life Sciences) pre-equilibrated with buffer A at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... Samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
-
bioRxiv - Biophysics 2022Quote: ... with a cut-off of 5 kDa and injected into HiLoad Superdex 200 prep grade chromatography (GE Healthcare) equilibrated with buffer C (20 mM Hepes-NaOH ...
-
bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was then transferred to a 5 mL tube containing 300 μL CaM-Sepharose resin (GE Healthcare) equilibrated in 50 mM HEPES (pH 7.2 ...
-
bioRxiv - Cell Biology 2024Quote: Time-lapse images were collected at 37°C with 5% CO2 using a DeltaVision ELITE microscope (Applied Precision). We used an apochromatic 100x objective lens (Olympus ...
-
bioRxiv - Cell Biology 2024Quote: ... and subsequently loaded onto a HiTrap SP (CEH-37)/Q (POT proteins) HP 5 mL column (GE Healthcare). An appropriate salt gradient was applied using 20 mM HEPES pH 7.5 buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.22 μm filter and applied to a 5 mL HiTrap Q HP column (GE Healthcare). The column was washed with 2 CV of buffer Q ...
-
bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
-
bioRxiv - Biochemistry 2024Quote: ... The eluents were pooled and directly loaded onto HiTrap Q HP 5 mL anion exchange column (GE Healthcare) pre-equilibrated with buffer D [50 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Biochemistry 2024Quote: ... The dialyzed solution was then loaded on a 5 ml HiTrap SP FF column (GE Healthcare, Pittsburgh, PA) and eluted using a linear gradient of NaCl (20-500 mM) ...
-
bioRxiv - Biochemistry 2024Quote: ... Excess HaloTag ligand was then removed by purification on desalting NAP-5 or PD-10 columns (GE Healthcare). To generate the HRP-his6Halo-hPKR-N conjugate ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 5% glycerol and further enriched with ion exchange Mono S HR 16/10 (SOURCETM 30Q GE Healthcare) to 95% purity ...
-
bioRxiv - Biochemistry 2024Quote: ... The eluate was diluted 10-fold and applied to a 5 mL HiTrap Q HP column (GE Healthcare) for ion exchange chromatography using a 50 mM HEPES pH 7.0 ...
-
bioRxiv - Immunology 2021Quote: ... 50 mM NaCl and 2 mM CaCl2) using a PD-10 desalting column (GE Healthcare, USA). Light chain of human enterokinase (10 μg/ml ...
-
Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... Total protein contents were assayed using the 2-D Quant Kit (GE Healthcare, Little Chalfont, UK), measuring OD480 in a microplate reader (Tecan Group ...
-
bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed 2 h at 4°C with Protein-A-conjugated sepharose beads (GE Healthcare) and anti-Myc antibody (9B11 ...
-
bioRxiv - Biophysics 2022Quote: ... The clarified lysates were loaded first with 2 ml of DEAE Sepharose fast flow (GE Healthcare) and the elutes obtained by a gradient of 100-500 mM NaCl in 50 mM NaH2PO4 ...
-
bioRxiv - Plant Biology 2021Quote: ... and the protein concentrations were determined using a 2-D Quant Kit (GE Healthcare; Piscataway, USA).
-
bioRxiv - Molecular Biology 2021Quote: ... 500 mM NaCl and 2 mM CaCl2 (IMAC A) using a Quixstand benchtop system (GE Healthcare) with a 10 kDa molecular weight cutoff (MWCO ...
-
bioRxiv - Microbiology 2023Quote: ... 2 mM TCEP) and applied to a HiLoad 16/600 Superdex 200 pg column (GE Healthcare), and protein purity was assessed by SDS-PAGE ...
-
bioRxiv - Systems Biology 2023Quote: ... proteins in 2% SDS were bound to paramagnetic beads (SeraMag Speed beads, GE Healthcare,#45152105050250,#651521050502) by addition of ethanol to a final concentration of 50% ...
-
bioRxiv - Molecular Biology 2022Quote: ... siGENOME Control Pool Non-Targeting #2 (cat# D-001206-14-20) was purchased from GE Healthcare. siRNA transfection was performed using Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2024Quote: ... the soluble fraction was incubated in batch for 2 h with glutathione Sepharose 4B (GE Healthcare), previously equilibrated in 50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 2 h with Glutathione Sepharose® 4 Fast Flow Beads (GE Healthcare) while rotating ...
-
bioRxiv - Biochemistry 2022Quote: ... the elution was concentrated to 2 mL and applied to a Superdex 75 column (GE Healthcare) for size exclusion chromatography ...
-
bioRxiv - Molecular Biology 2023Quote: ... the samples were concentrated using the 2-D Clean-Up Kit (80648451, GE Healthcare, Chicago, IL), reduced in 1 × Laemmli’s sample buffer (32.9 mM Tris HCl ...
-
bioRxiv - Plant Biology 2023Quote: ... The protein concentration was determined with 2-D Quant Kit (GE Healthcare Life Sciences, Westborough, USA) using Bovine Serum Albumin (BSA ...
-
bioRxiv - Physiology 2024Quote: ... and supernatants were then aliquoted into 2 tubes containing thiopropyl sepharose beads (GE Healthcare Life Sciences), with or without freshly-prepared HA (hydroxylamine 0.8 M ...
-
bioRxiv - Biochemistry 2024Quote: ... and 2 mM DTT and loaded on a Source™ 15PHE 4.6/100 PE (GE Healthcare). The sample was eluted with a 1.75 to 0.8 M linear gradient of (NH4)2SO4 for 23 CV at a flow rate of 1 ml.min-1 ...
-
bioRxiv - Microbiology 2021Quote: ... albicans Phr1 and Pga4 gene without the sequence encoding signal peptide and the GPI anchor were constructed using primers listed in Supplementary Table 3 and cloned into the bacterial expression vector pGEX6P1 (GE healthcare, Stockholm, Sweden). Plasmids were then transformed into E ...
-
bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...