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Citations for GE Life Sciences :
3101 - 3150 of 3622 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare).
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bioRxiv - Bioengineering 2024Quote: ... the soluble fraction of the cell lysate was passed through a 0.45-micron filter and then loaded onto a HisTrap HP 5 ml (GE Healthcare) column equilibrated with buffer A (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: The Ni-NTA elution was immediately loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare, Chicago, IL, USA) that was equilibrated with 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was filtered through an 0.8 µm membrane and loaded onto a 5 mL HisTrap HP column (GE Healthcare), pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... and the sample was loaded on a 5 ml HiTrap™ Phenyl Sepharose High-Performance column (GE Healthcare, Munich, Germany). The sample was eluted at 1 M (NH4)2SO4 at a flow rate of 1 ml.min-1 ...
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bioRxiv - Biophysics 2024Quote: ... RbmB was purified from the cleared lysate by loading the supernatant onto a 5 mL His-trap HF (GE Healthcare) equilibrated with high-salt TBS buffer (20 mM Tris pH 7.6 ...
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bioRxiv - Bioengineering 2024Quote: ... The eluate was then exchanged into 20 mM Tris HCl pH 8.0 by dialysis and loaded onto a 5 mL Q-HP column (GE Healthcare). The different forms were isolated by using 50 column volumes (250 mL ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified by salt gradient ion exchange chromatography using a Mono Q 5/50 GL column (GE healthcare) and Gradient buffers (20 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... The resultant supernatant was filtered through a 0.8 μm membrane before being loaded onto 5 mL HisTrap FF nickel column (GE Healthcare) pre-equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble material was subsequently filtered using a 0.22 mm pore size filter and was loaded onto a 5 ml HisTrap HP column (GE Healthcare), which was pre-equilibrated with lysis buffer and immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were centrifuged and filtered prior to successive FPLC purification on HisTrap HP (5 mL, GE Healthcare, 17-1154-01), HiTrap Q HP (5 mL ...
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bioRxiv - Developmental Biology 2021Quote: Single cell suspension was collected by centrifugation (3 min at 300 g) and total RNA was extracted by Illustra™ RNAspin RNA Isolation Kit (GE Healthcare GE25-0500-72) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution in 3% milk overnight at 4°C before incubation with secondary HRP-linked sheep anti mouse (NA931-1ML, GE Healthcare Life Sciences, Buckinghamshire, UK) at a 1:3000 dilution in 5% milk for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... chromosomal DNA was purified and amplified to 5.4 - 7.9 μg by multiple displacement amplification (Supplementary Table 3) using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, United Kingdom) as described by Šimková et al ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times for 10 min in TBS-T and incubated with the secondary horse radish peroxidase (HRP) coupled antibody (GE Healthcare, NXA931V; in a 1:3000 dilution) for two hours under agitation at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... Polyacrylamide gels were polymerized between two glass coverslips treated with 2% dimethyldichlorosilane (Plus One Repel Silane, GE Healthcare). For 0.6 kPa gels ...
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bioRxiv - Biochemistry 2020Quote: ... were subjected to the short column with 2 ml Sephacryl S-500 resin (GE Healthcare Life Sciences, IL) equilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... dried for 2 h at 65°C and radiolabelled products visualised using a Typhoon FLA-700 (GE Healthcare) following exposure to a phosphorimaging plate for 24-72 h.
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bioRxiv - Microbiology 2020Quote: ... concentrated to 2 mg/ml by spin filtration and then run on a Superdex 200 column (GE Healthcare). MALS was conducted using a MiniDAWN and Optilab system (Wyatt Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) equilibrated in Dynein-lysis buffer and incubated for 3-4 h with rotation along the long axis of the tube ...
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bioRxiv - Biophysics 2022Quote: ... were further subjected to Ni-NTA purification by mixing with 2 mL of Ni-Sepharose resin (GE Healthcare) for 4 hrs and the bound proteins were eluted with a gradient of 100-500 mM imidazole in 10 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... were further subjected to Ni-NTA purification by mixing with 2 mL of Ni-Sepharose resin (GE Healthcare) for 4 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM DTE (IbpA) or applied to a HiLoad™ 16/600 Superdex™ 200pg column (GE Healthcare) in 20 mM Hepes pH 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: For mass spectrometry the beads were incubated with 150 µL the elution buffer (GE Healthcare, 2-1042-025) for 10 min at 4 °C while rotating ...
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bioRxiv - Molecular Biology 2022Quote: ... 93-207 Bcl-2) with a C-terminal 6His tag and purified using Ni2+ IMAC (GE Healthcare, Fastflow). Similarly ...
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bioRxiv - Immunology 2022Quote: The binding kinetics of nanobodies to SARS-CoV-2 RBD were analyzed using SPR (Biacore 8K; GE Healthcare). The nanobodies were captured by the ProA sensor chip and serial dilutions of SARS-CoV-2 RBD were then flowed through at a rate of 30 μL/min in PBS buffer (with 0.05% Tween20 ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL Glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the plate was blocked with 200 μl of a 2% (w/v) ECL Advance Blocking Reagent (GE Healthcare) solution in PBS-T (PBS supplemented with 0.1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed with ECL 2 western blot substrate and scanned with an ImageQuant LAS 4000 (GE Healthcare), and signals analyzed with ImageQuant TL 7.0 software (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... Cell-free supernatant was applied to the column at 2 mL/min using an AKTA Pure (GE Healthcare), washed ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (1×PBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... proteins in 2% SDS were bound to paramagnetic beads (SeraMag Speed beads, GE Healthcare, CAT#45152105050250, CAT#651521050502) by addition of ethanol to a final concentration of 50% ...
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bioRxiv - Genetics 2024Quote: ... 2 M NaCl ) and purified by size exclusion chromatography on a Superdex 200 gel filtration column (GE Healthcare). To form nucleosome arrays ...
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Biophysics 2021Quote: ... the supernatant was filtered using a 0.45 µm filter and then loaded onto a 5 ml HisTrap™ HP nickel sepharose column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Cell Biology 2020Quote: GST-tagged proteins (Retromer, SNX3, WASHC2C-5 and WASHC2C-21) were purified by incubating clarified lysate with glutathione (GSH) Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μg of MCF10A cell lysate and 5 μg of individual GST-tagged recombinant proteins were mixed and incubated with glutathione Sepharose 4b beads (GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 or 10 µg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the dialyzed sample was loaded on an anion exchange HiTrap Q HP 5 mL column (GE healthcare life sciences, Boston, Massachusetts) pre-equilibrated with 20 mM bis-Tris pH 5.5 ...
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bioRxiv - Biophysics 2022Quote: All samples were pre-reduced with 5 mM DTT for one hour followed by desalting using a PD10 column (GE Healthcare) into 20 mM tris pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... Cell disruption was achieved by sonication (Branson Sonifier SFX250) and the soluble fraction was subjected to immobilized metal affinity purification using a 5 ml Ni-NTA column (GE healthcare) connected to a FPLC system (GE healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... The eluted fractions were concentrated to 5 ml and loaded onto a HiLoad 26/600 Superdex 200 prep grade (GE Healthcare) equilibrated with the SEC buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... 1.5 μM PCNA trimer and 20 μM TTP was loaded onto a Superdex 200 increase 3.2/300 column (GE Life Sciences) equilibrated with a buffer comprising [25 mM HEPES (pH 7.5) ...
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bioRxiv - Bioengineering 2020Quote: ... The homogenate was centrifuged as before and the supernatant was purified on an AKTA Pure FPLC equipped with a HiTrap Q 5 mL anion exchange column (GE Healthcare). Solvent A was 20 mM tris pH 8.5 ...
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bioRxiv - Bioengineering 2020Quote: ... and the supernatant was purified using an AKTA Pure FPLC equipped with a HisTrap FF crude 5 mL column (GE Healthcare). Solvent A was 20 mM tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Tag and TEV protease removal was achieved by re-loading the dialyzed sample onto the 5 mL HisTrap Excel column (GE Healthcare), and collecting the unbound tagless sample ...
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bioRxiv - Biochemistry 2020Quote: ... The protein of interest (NT-mini or NT*-mini) was then eluted into a 5 ml Ni-IMAC HisTrap Excel column (GE Healthcare) by further increasing the NaCl concentration to 600 mM (buffer NT.B) ...